##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	654949
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.691313369437925	28.0	25.0	31.0	16.0	33.0
2	27.52459046429569	30.0	25.0	31.0	16.0	33.0
3	27.584216480977908	30.0	25.0	31.0	16.0	33.0
4	30.48311700605696	33.0	28.0	35.0	19.0	37.0
5	31.845833797746085	33.0	32.0	35.0	26.0	37.0
6	31.21097673253948	35.0	30.0	35.0	17.0	37.0
7	31.889438719656034	33.0	30.0	35.0	26.0	35.0
8	33.197053511036735	35.0	33.0	35.0	28.0	37.0
9	34.390212062313246	37.0	33.0	38.0	28.0	39.0
10	33.80381373206158	35.0	32.0	39.0	27.0	39.0
11	33.85094869982243	37.0	32.0	39.0	26.0	39.0
12	33.35461539753477	35.0	31.0	38.0	25.0	39.0
13	33.43240160684267	35.0	32.0	39.0	24.0	39.0
14	34.204270866891925	37.0	32.0	39.0	24.0	40.0
15	35.16862228967447	37.0	33.0	39.0	27.0	41.0
16	35.25860028796135	37.0	33.0	39.0	27.0	41.0
17	34.801708224609854	36.0	33.0	39.0	27.0	40.0
18	34.56001459655637	36.0	33.0	39.0	27.0	40.0
19	33.90029299991297	36.0	32.0	38.0	25.0	40.0
20	33.857990469486936	35.0	32.0	38.0	27.0	40.0
21	34.33731328698876	35.0	33.0	39.0	27.0	40.0
22	34.5869021862771	35.0	33.0	39.0	27.0	40.0
23	34.9567157137426	35.0	33.0	39.0	29.0	40.0
24	34.66322568627481	35.0	33.0	39.0	27.0	40.0
25	33.94481097001446	35.0	33.0	39.0	24.0	40.0
26	33.812770154622726	35.0	32.0	39.0	24.0	40.0
27	34.29146391551098	35.0	33.0	39.0	25.0	40.0
28	34.184971654281476	35.0	33.0	39.0	25.0	40.0
29	34.24099433696364	35.0	33.0	39.0	25.0	40.0
30	33.751838692783714	35.0	33.0	39.0	23.0	40.0
31	33.6292184582311	35.0	33.0	39.0	23.0	40.0
32	33.15445477434121	35.0	32.0	39.0	21.0	40.0
33	32.999992365817796	35.0	32.0	39.0	18.0	40.0
34	32.673838726374115	35.0	32.0	39.0	15.0	40.0
35	32.658152008782366	36.0	32.0	39.0	15.0	40.0
36	32.13341954869769	35.0	31.0	39.0	12.0	40.0
37	32.27281513522427	35.0	31.0	39.0	10.0	40.0
38	32.14101861366305	35.0	31.0	39.0	10.0	40.0
39	32.02592720959952	35.0	31.0	39.0	10.0	40.0
40	31.971358075208908	35.0	31.0	39.0	10.0	40.0
41	31.599993281919662	35.0	30.0	39.0	10.0	40.0
42	31.79221435562158	35.0	30.0	39.0	10.0	40.0
43	31.58539825238301	35.0	30.0	39.0	10.0	40.0
44	31.64961699307885	35.0	30.0	39.0	10.0	40.0
45	31.744048773263263	35.0	30.0	39.0	10.0	40.0
46	31.526164632666056	35.0	30.0	39.0	10.0	40.0
47	31.621132332441153	35.0	30.0	39.0	10.0	40.0
48	31.506804346597978	35.0	30.0	39.0	10.0	40.0
49	31.567670154470044	35.0	30.0	39.0	10.0	40.0
50	31.377246167258825	35.0	30.0	39.0	10.0	40.0
51	29.353488592241533	33.0	25.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	41.0
10	36.0
11	35.0
12	25.0
13	56.0
14	91.0
15	183.0
16	470.0
17	996.0
18	1937.0
19	3262.0
20	4856.0
21	6809.0
22	9250.0
23	12081.0
24	15910.0
25	19473.0
26	21535.0
27	21423.0
28	21610.0
29	24432.0
30	29807.0
31	37437.0
32	48394.0
33	58852.0
34	66320.0
35	63529.0
36	70899.0
37	69554.0
38	41255.0
39	4377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.1881612156061	19.33112349205816	20.93918763140336	23.541527660932378
2	34.738430015161484	29.902633640176564	23.05553562185758	12.303400722804371
3	24.920871701460726	27.790102740824096	35.604757011614645	11.684268546100537
4	21.81864542124654	22.368459223542597	42.016248593401926	13.796646761808933
5	25.79849728757506	25.277082643076028	34.790647821433424	14.133772247915486
6	17.70214169347537	37.03570812383865	34.75675205244988	10.505398130236095
7	67.46372618326008	4.217580300145507	25.94644773867889	2.3722457779155324
8	65.55792893797837	10.637316798712572	19.468996822653367	4.3357574406556845
9	61.89245269478998	4.490120604810451	20.235010664952537	13.382416035447035
10	40.7676017521975	20.61504025504276	26.540539797755248	12.076818195004495
11	35.54643185957991	19.386394971211498	31.270984458331867	13.796188710876725
12	30.647882506882212	17.24271660846875	35.19983998754101	16.90956089710802
13	20.737950588519105	26.78010043530107	38.90318177445877	13.578767201721051
14	15.776648258108647	33.273430450309874	32.82438785310001	18.12553343848147
15	11.56838166025141	21.584886762175376	52.55874884914703	14.287982728426183
16	13.93772644892961	19.875746050455838	43.666758785798585	22.519768714815964
17	14.699617832838893	19.462278742314286	39.370393725312965	26.46770969953386
18	17.437082887369858	21.504727849038627	39.408717319974535	21.649471943616984
19	20.17561672740931	23.83284805381793	35.04181241592857	20.949722802844192
20	24.3037244121298	20.98819908114983	39.21740471395483	15.490671792765543
21	20.353798540038994	26.81277473513205	36.173045534843176	16.660381189985785
22	19.046063128579476	21.00697916937044	35.87592316348296	24.071034538567122
23	16.55777778117075	29.348392012202478	34.746216881009055	19.34761332561772
24	19.69756423782615	20.277914768936206	38.30573067521288	21.718790318024762
25	16.105070776503208	27.004850759372108	36.39596365518536	20.494114808939322
26	16.251799758454474	25.665051782657887	35.906612575941026	22.17653588294661
27	19.249132375192573	27.625051721584427	33.77530158836795	19.35051431485505
28	13.546550952822281	26.78819266843678	42.115187594759284	17.550068783981654
29	18.519151872893918	24.082485811872374	37.62995286655907	19.76840944867463
30	20.417009568683973	25.77864841384596	35.996695925942326	17.807646091527737
31	23.658483332289997	25.692534838590486	30.661318667560373	19.987663161559144
32	26.039584761561585	26.484199533093417	32.14998419724284	15.32623150810216
33	24.497327272810555	24.19501365755196	28.903624557026582	22.404034512610906
34	23.643825702459274	27.565963151329342	30.958746406208725	17.831464740002655
35	23.86674382280147	23.14225993168934	30.92958383019136	22.061412415317832
36	24.953698684935773	28.18830168455865	29.893930672464574	16.964068958041008
37	23.545039384745987	25.907055358508828	34.63613197363459	15.911773283110595
38	24.957668459681592	28.179446033202588	26.037141823256466	20.825743683859354
39	23.816358220258373	24.21852693873874	33.32503752200553	18.64007731899736
40	28.316250578289303	21.605957105057037	31.38015326384192	18.697639052811745
41	21.39220000335904	23.806586467037892	32.1176152647	22.68359826490307
42	25.316169655957943	24.22936747746771	30.656127423661992	19.79833544291235
43	24.433505509589295	23.548551108559597	29.952561191787453	22.065382190063655
44	22.488010516849403	25.754677081726975	31.304116809095063	20.453195592328562
45	19.414946812652587	26.692154656316752	29.029435879740255	24.863462651290405
46	25.756051234523603	28.010119871928957	27.99729444582708	18.236534447720356
47	20.394107022073474	24.205548828992793	36.1270877579781	19.27325639095563
48	23.560460432797058	24.224939651789683	28.381751861595326	23.83284805381793
49	20.859944820131034	21.04576081496422	35.8334771104315	22.26081725447325
50	21.285779503442253	23.72505340110451	31.76003017028807	23.229136925165164
51	19.03995578281668	24.946980604596693	28.30327246854335	27.709791144043276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	210.0
2	364.0
3	7268.0
4	14172.0
5	11772.5
6	9373.0
7	9456.0
8	9539.0
9	9921.5
10	10304.0
11	10059.0
12	9814.0
13	9193.5
14	8573.0
15	8159.0
16	7745.0
17	7177.0
18	6609.0
19	6156.5
20	5704.0
21	5314.5
22	4925.0
23	4548.5
24	4172.0
25	4870.5
26	5536.5
27	5504.0
28	5909.0
29	6314.0
30	6643.0
31	6972.0
32	7732.0
33	8492.0
34	9991.0
35	11490.0
36	12225.5
37	12961.0
38	14978.0
39	16995.0
40	22277.0
41	27559.0
42	32593.5
43	37628.0
44	42664.5
45	47701.0
46	76204.5
47	104708.0
48	82033.5
49	59359.0
50	55279.5
51	51200.0
52	43668.5
53	36137.0
54	32316.0
55	28495.0
56	26596.5
57	24698.0
58	21836.5
59	18975.0
60	18329.0
61	17683.0
62	16163.5
63	14644.0
64	12124.0
65	9604.0
66	6867.0
67	4130.0
68	3500.0
69	2870.0
70	2327.5
71	1785.0
72	1357.0
73	929.0
74	798.5
75	460.5
76	253.0
77	200.0
78	147.0
79	97.0
80	47.0
81	35.0
82	23.0
83	28.5
84	34.0
85	22.0
86	10.0
87	9.5
88	9.0
89	6.0
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	654949.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.055437454173806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.94983211196602	26.070987604415524
2	7.860118111898595	4.881988128129711
3	2.530101441623157	2.357202212191288
4	1.1444668764367683	1.4216767799822287
5	0.6686397472717259	1.0382449925385833
6	0.39620808265341384	0.7382649197808733
7	0.27197411078307693	0.5912392490604869
8	0.20967693112952998	0.5209287056220981
9	0.17148601377709466	0.4793015857608136
>10	1.5877577801886038	11.742711615733691
>50	0.6547943276296148	14.709707683505362
>100	0.5469163349550002	24.23019200603502
>500	0.0020070324218532123	0.4677811701894374
>1k	0.0040140648437064245	3.2593533433885615
>5k	5.017581054633031E-4	0.8437824905315799
>10k+	0.001505274316389909	6.646637513134728
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	15987	2.4409534177470307	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	14265	2.178032182658497	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	12403	1.893735237400164	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5415	0.82678193263903	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	4184	0.6388283667888645	No Hit
GAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	3744	0.5716475633980661	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC	2969	0.45331773924381896	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	2607	0.39804626009048033	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2371	0.3620129200899612	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATGGGTT	1958	0.29895457508905277	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTA	1947	0.2972750550042828	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	1137	0.17360130330758577	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGT	851	0.12993378110356682	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT	755	0.11527615127284721	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	734	0.11206979474737727	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTAT	662	0.10107657237433754	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20490145034193502	0.0
2	0.0	0.0	0.0	1.2120027666276305	0.0
3	0.0	0.0	0.0	1.397818761460816	0.0
4	0.0	0.0	0.0	2.2435334659645254	0.0
5	0.0	0.0	0.0	4.92725387778285	0.0
6	0.0	0.0	0.0	5.421185466349288	0.0
7	0.0	0.0	0.0	6.140478113563041	0.0
8	0.0	0.0	0.0	7.364542888072201	0.0
9	0.0	0.0	0.0	7.713883065704352	0.0
10	0.0	0.0	0.0	10.68281652464543	0.0
11	0.0	0.0	0.0	11.341035714231184	0.0
12	0.0	0.0	0.0	14.703129556652502	0.0
13	0.0	0.0	0.0	15.461509216748174	0.0
14	0.0	0.0	0.0	15.740462234464058	0.0
15	0.0	0.0	0.0	16.280351599895564	0.0
16	0.0	0.0	0.0	16.783902258038413	0.0
17	0.0	0.0	0.0	17.194621260586704	0.0
18	3.0536728813999257E-4	0.0	0.0	17.903836787291834	0.0
19	3.0536728813999257E-4	0.0	0.0	18.48693562399515	0.0
20	3.0536728813999257E-4	0.0	0.0	18.813373255016803	0.0
21	3.0536728813999257E-4	0.0	0.0	19.039192364596328	0.0
22	3.0536728813999257E-4	0.0	0.0	19.2902042754474	0.0
23	3.0536728813999257E-4	0.0	0.0	19.494494991213056	0.0
24	3.0536728813999257E-4	0.0	0.0	19.68290660799543	0.0
25	3.0536728813999257E-4	0.0	0.0	19.840934179607878	0.0
26	3.0536728813999257E-4	0.0	0.0	20.003084209610215	0.0
27	3.0536728813999257E-4	0.0	0.0	20.189205571731538	0.0
28	3.0536728813999257E-4	0.0	0.0	20.333033564445476	0.0
29	3.0536728813999257E-4	0.0	0.0	20.482968902922213	0.0
30	3.0536728813999257E-4	0.0	0.0	20.67565566173855	0.0
31	3.0536728813999257E-4	0.0	0.0	20.855058943520792	0.0
32	3.0536728813999257E-4	0.0	0.0	21.0265226758114	0.0
33	3.0536728813999257E-4	0.0	0.0	21.183939512847566	0.0
34	3.0536728813999257E-4	0.0	0.0	21.35754081615515	0.0
35	3.0536728813999257E-4	0.0	0.0	21.535417261496697	0.0
36	7.634182203499814E-4	0.0	0.0	21.706728310143234	0.0
37	7.634182203499814E-4	0.0	0.0	21.878039358789767	0.0
38	7.634182203499814E-4	0.0	0.0	22.030723002859766	0.0
39	7.634182203499814E-4	0.0	0.0	22.20615650989619	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.88896E-5	45.000004	25
AGCCGTG	25	3.88896E-5	45.000004	31
CGACGGT	25	3.88896E-5	45.000004	28
CGTGCAT	35	1.2108649E-7	45.000004	9
ACCGCAT	35	1.2108649E-7	45.000004	33
CGTTGGA	25	3.88896E-5	45.000004	35
TTCGTCG	35	1.2108649E-7	45.000004	1
GTTAGCG	35	1.2108649E-7	45.000004	1
ATAGACT	35	1.2108649E-7	45.000004	34
CTGACTA	35	1.2108649E-7	45.000004	11
CGACACC	25	3.88896E-5	45.000004	38
TCGATAG	25	3.88896E-5	45.000004	1
ACGGGCC	35	1.2108649E-7	45.000004	5
AAACGTC	25	3.88896E-5	45.000004	12
ACGATAG	50	2.1827873E-11	45.000004	1
CTAACGG	25	3.88896E-5	45.000004	2
TCGGAGT	25	3.88896E-5	45.000004	41
CCGTAGA	25	3.88896E-5	45.000004	42
TCGGACC	35	1.2108649E-7	45.000004	17
TCTACCG	25	3.88896E-5	45.000004	38
>>END_MODULE