##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	430154
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.346622372452657	30.0	25.0	31.0	16.0	33.0
2	27.861830879173507	31.0	25.0	33.0	16.0	33.0
3	27.827891871283306	31.0	25.0	33.0	16.0	34.0
4	31.953181883697468	35.0	32.0	35.0	19.0	37.0
5	33.16399475536668	35.0	32.0	35.0	28.0	37.0
6	32.07447332815689	35.0	32.0	35.0	25.0	37.0
7	33.787252937320126	35.0	33.0	35.0	31.0	37.0
8	33.570665389604656	35.0	33.0	37.0	28.0	37.0
9	36.21947023624097	39.0	35.0	39.0	31.0	39.0
10	35.43331225561078	37.0	34.0	39.0	31.0	39.0
11	35.620410364660096	37.0	35.0	39.0	31.0	39.0
12	35.604292881154194	37.0	34.0	39.0	30.0	39.0
13	35.44037251774946	37.0	34.0	39.0	30.0	39.0
14	36.105469204052504	38.0	35.0	40.0	30.0	41.0
15	36.615391231977384	38.0	35.0	40.0	31.0	41.0
16	36.39109249245619	38.0	34.0	40.0	32.0	41.0
17	35.907905075856554	37.0	34.0	40.0	30.0	41.0
18	35.91944977845144	37.0	34.0	39.0	30.0	40.0
19	35.42374591425396	37.0	34.0	39.0	29.0	40.0
20	35.50051144473839	37.0	34.0	39.0	30.0	40.0
21	35.8116674493321	37.0	34.0	39.0	30.0	40.0
22	36.2061773225403	38.0	35.0	40.0	31.0	41.0
23	36.36894461053483	38.0	35.0	40.0	31.0	41.0
24	36.35526811328036	38.0	35.0	40.0	31.0	41.0
25	35.67471649688251	37.0	34.0	40.0	30.0	41.0
26	35.80391673679659	37.0	34.0	40.0	30.0	41.0
27	36.00566773760095	38.0	35.0	40.0	31.0	41.0
28	35.83586575970467	38.0	35.0	40.0	30.0	41.0
29	35.88438094263915	38.0	35.0	40.0	30.0	41.0
30	35.351206777107734	37.0	34.0	40.0	29.0	41.0
31	35.36394872534023	37.0	34.0	40.0	29.0	41.0
32	35.01481562417181	37.0	34.0	40.0	27.0	41.0
33	34.875453907205326	38.0	34.0	40.0	25.0	41.0
34	34.54381454083886	38.0	34.0	40.0	23.0	41.0
35	34.539774127405536	38.0	34.0	40.0	23.0	41.0
36	34.044077237454495	37.0	34.0	40.0	20.0	41.0
37	34.20499402539556	37.0	34.0	40.0	21.0	41.0
38	34.03823746844154	37.0	33.0	40.0	20.0	41.0
39	33.93592294852541	37.0	33.0	40.0	19.0	41.0
40	33.9808742915328	37.0	33.0	40.0	20.0	41.0
41	33.5757775122398	37.0	33.0	40.0	18.0	41.0
42	33.76905945312609	37.0	33.0	40.0	18.0	41.0
43	33.607066306485585	37.0	33.0	40.0	18.0	41.0
44	33.77681481515922	37.0	33.0	40.0	18.0	41.0
45	33.87400326394733	37.0	34.0	40.0	20.0	41.0
46	33.64226532823128	37.0	33.0	40.0	18.0	41.0
47	33.557656095258906	36.0	33.0	40.0	19.0	41.0
48	33.62761011172743	36.0	33.0	40.0	19.0	41.0
49	33.676141567903585	36.0	33.0	40.0	19.0	41.0
50	33.452286855405276	36.0	33.0	40.0	18.0	41.0
51	31.42268117929858	34.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	14.0
11	9.0
12	17.0
13	22.0
14	28.0
15	53.0
16	93.0
17	228.0
18	521.0
19	1042.0
20	1918.0
21	2689.0
22	3381.0
23	4167.0
24	5363.0
25	7353.0
26	9009.0
27	9430.0
28	9098.0
29	9740.0
30	11791.0
31	15946.0
32	22585.0
33	34825.0
34	44263.0
35	44464.0
36	57822.0
37	70349.0
38	53252.0
39	10673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.40531530568122	21.227513867126657	22.67838960000372	17.688781227188404
2	33.40547803809798	29.712149602235478	23.393714809114876	13.488657550551665
3	25.651975804014377	28.99078004621601	32.304941951022194	13.052302198747427
4	22.64747044081887	23.79752367756664	38.90211412656863	14.652891755045866
5	25.928620912510404	27.276975222827172	31.607284832873805	15.187119031788615
6	18.525690799109157	37.54376339636502	31.349005239983818	12.581540564542001
7	68.825350920833	5.638213291053901	21.82660163569326	3.709834152419831
8	65.9614928606964	10.204949855168149	16.545004812230037	7.28855247190541
9	61.3257112568987	6.260083597967239	18.941588361377555	13.472616783756516
10	39.68462457631453	22.350135067905914	24.11787406370742	13.847366292072142
11	33.36340008462086	21.484166135849023	29.118176281052833	16.034257498477288
12	28.214546418259506	18.70283665849905	33.24832501848176	19.83429190475969
13	22.7448774159952	26.175741711108113	36.586199361159025	14.493181511737657
14	15.959633061647688	34.253778879192105	30.038079385522394	19.74850867363781
15	11.55818613798779	23.360005951356953	47.73383485914347	17.347973051511783
16	13.819701781222538	23.14240946265756	39.26152029273237	23.77636846338753
17	14.48434746625627	23.51832134537863	35.071625510863555	26.925705677501544
18	17.20755822333397	23.128693444673303	36.316528499095675	23.347219832897057
19	19.210096849035462	26.516084937022555	32.09966663102052	22.174151582921464
20	24.10950496798821	23.659898547961895	34.927026134826136	17.303570349223765
21	20.03608010154503	29.707732579494785	32.75710559474049	17.499081724219696
22	18.077246753488286	23.545520906466056	32.66830019016445	25.708932149881203
23	16.83071644108854	30.310772421039907	31.453618936473916	21.404892201397637
24	19.688530154316826	22.764637780887774	34.0340901165629	23.51274194823249
25	16.363441930099455	29.020304356114323	31.682374219465586	22.93387949432064
26	16.143055742826988	27.556177555015182	33.610985832980745	22.689780869177085
27	20.588672893893815	28.430050633029104	30.52929880926366	20.451977663813427
28	14.0849556205452	28.802010442771657	37.31617048777879	19.796863448904347
29	18.775368821398846	26.09739767618109	34.08174746718617	21.045486035233893
30	22.506358187997787	27.513402176894786	31.413400782045496	18.566838853061927
31	23.70546362465536	27.782143139433785	26.74646754418185	21.76592569172901
32	25.66243717366339	28.56395616453642	28.834789401005224	16.938817260794973
33	25.62570614245131	24.96175788206084	27.047290040311147	22.365245935176702
34	23.705696099536443	27.90651720081645	29.32461397545995	19.06317272418715
35	24.815531181855803	23.96513806683188	29.174435202276396	22.044895549035925
36	25.944661679305554	28.47561570972256	28.650901770063747	16.92882084090814
37	25.172612599208655	25.370448723015475	33.219033183464525	16.237905494311338
38	26.231303207688413	27.09146026771807	25.749615254071795	20.92762127052172
39	25.672433593550213	22.89761341287074	31.819999349070333	19.60995364450871
40	30.638097053613357	20.52869437457283	30.20476387526328	18.628444696550538
41	23.399759156023194	22.682341672982233	30.0806222887617	23.837276882232874
42	26.60768004017166	23.456483027008932	30.09666305555685	19.83917387726256
43	25.268857199979543	22.197166596149287	30.240565006950998	22.293411196920175
44	23.34884715706468	24.870162778911737	30.012739623483682	21.768250440539898
45	21.15963120184864	23.942355528485145	28.92777935344086	25.97023391622535
46	27.444822086973502	25.619894270424084	27.577565244075373	19.357718398527037
47	21.274241318225563	23.274687669997256	34.895642025879106	20.555428985898075
48	23.76102512123565	22.480321001315808	28.795501146101167	24.963152731347378
49	22.615388907228574	18.801173533199737	35.171124759969686	23.412312799602002
50	21.51090074717427	21.12917699242597	32.54950552592793	24.81041673447184
51	19.61530056677376	21.766158166610097	28.432375381839993	30.186165884776152
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	646.0
1	667.5
2	689.0
3	5002.0
4	9315.0
5	6362.5
6	3410.0
7	3483.5
8	3557.0
9	3743.5
10	3930.0
11	3836.5
12	3743.0
13	3656.5
14	3570.0
15	3432.5
16	3295.0
17	3131.0
18	2967.0
19	2795.0
20	2623.0
21	2629.0
22	2635.0
23	2508.0
24	2381.0
25	2843.0
26	3451.5
27	3598.0
28	4076.0
29	4554.0
30	4788.0
31	5022.0
32	5334.0
33	5646.0
34	6174.5
35	6703.0
36	7342.0
37	7981.0
38	8999.5
39	10018.0
40	13077.0
41	16136.0
42	19451.5
43	22767.0
44	26621.5
45	30476.0
46	46755.0
47	63034.0
48	57791.0
49	52548.0
50	48670.0
51	44792.0
52	37153.0
53	29514.0
54	25514.0
55	21514.0
56	19094.5
57	16675.0
58	14272.5
59	11870.0
60	11455.5
61	11041.0
62	9895.5
63	8750.0
64	7107.0
65	5464.0
66	3851.5
67	2239.0
68	1898.0
69	1557.0
70	1262.0
71	967.0
72	753.0
73	539.0
74	463.0
75	284.5
76	182.0
77	119.5
78	57.0
79	40.5
80	24.0
81	16.0
82	8.0
83	11.0
84	14.0
85	9.0
86	4.0
87	3.5
88	3.0
89	3.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	430154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.881254802883014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.96775190817381	24.857097649924576
2	9.48255436776547	6.046314639618443
3	3.687366150548658	3.5267357939150292
4	1.9187559430526773	2.44689388500034
5	1.070969694873226	1.7071928864209596
6	0.7530951734137148	1.440577146865441
7	0.4764491322123655	1.0632857329272447
8	0.3669664057525541	0.9359479588716267
9	0.27593477833090635	0.7917432279250202
>10	2.9903950589490162	22.95375304926855
>50	0.9033548454272542	19.83602999098526
>100	0.09246361537273276	4.316737505026468
>500	0.004403029303463465	0.8363956739726638
>1k	0.006604543955195197	3.543042261997768
>5k	0.002935352868975643	5.698252597280615
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	6663	1.5489801326966621	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	6511	1.5136439507711192	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5627	1.3081361558883562	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	5555	1.2913979644499411	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	2856	0.6639482603904648	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATGGGTT	1888	0.4389125754962176	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	1874	0.4356579271609703	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	1704	0.39613719737582354	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1567	0.3642881386666171	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	1450	0.3370885775791926	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC	1412	0.32825453209780686	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1211	0.28152708099889806	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTA	1182	0.2747853094473142	No Hit
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	663	0.15413084616207218	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	656	0.1525035219944485	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT	611	0.14204215234543907	No Hit
ACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	574	0.13344058174514242	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	561	0.13041840829098417	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGT	510	0.11856218935544015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4874998256438392	0.0
2	0.0	0.0	0.0	1.96464522008397	0.0
3	0.0	0.0	0.0	2.53699837732533	0.0
4	0.0	0.0	0.0	3.6254457705844882	0.0
5	0.0	0.0	0.0	6.725033360145436	0.0
6	0.0	0.0	0.0	8.062693825932108	0.0
7	0.0	0.0	0.0	9.256452340324628	0.0
8	0.0	0.0	0.0	11.609563086708482	0.0
9	0.0	0.0	0.0	12.373475545967258	0.0
10	0.0	0.0	0.0	15.730412828893838	0.0
11	0.0	0.0	0.0	17.50675339529564	0.0
12	0.0	0.0	0.0	20.946916685652116	0.0
13	0.0	0.0	0.0	21.80405157222762	0.0
14	0.0	0.0	0.0	22.163457738391365	0.0
15	0.0	0.0	0.0	22.9176062526444	0.0
16	0.0	0.0	0.0	23.876100187374753	0.0
17	0.0	0.0	0.0	24.7920512188658	0.0
18	0.0	0.0	0.0	26.28314510617128	0.0
19	0.0	0.0	0.0	27.410880754334496	0.0
20	0.0	0.0	0.0	28.076223864011492	0.0
21	0.0	0.0	0.0	28.654388893280082	0.0
22	0.0	0.0	0.0	29.225347201234907	0.0
23	0.0	0.0	0.0	29.732142442009142	0.0
24	0.0	0.0	0.0	30.143855456417935	0.0
25	0.0	0.0	0.0	30.51116576853871	0.0
26	0.0	0.0	0.0	30.860343039934534	0.0
27	0.0	0.0	0.0	31.231140475271648	0.0
28	0.0	0.0	0.0	31.563347080347967	0.0
29	0.0	0.0	0.0	31.894158836137755	0.0
30	0.0	0.0	0.0	32.278672289459124	0.0
31	0.0	0.0	0.0	32.65202694848821	0.0
32	0.0	0.0	0.0	32.98795315166196	0.0
33	0.0	0.0	0.0	33.309233437327094	0.0
34	0.0	0.0	0.0	33.61400800643491	0.0
35	0.0	0.0	0.0	33.960395579257664	0.0
36	0.0	0.0	0.0	34.28493051325804	0.0
37	0.0	0.0	0.0	34.647591327757034	0.0
38	0.0	0.0	0.0	34.942369476978016	0.0
39	0.0	0.0	0.0	35.29805604504433	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	160	0.0	45.000004	4
TTTAGCG	20	7.028358E-4	45.000004	1
AAGTAAC	20	7.028358E-4	45.000004	21
TAGAGAT	20	7.028358E-4	45.000004	28
CTCACTA	20	7.028358E-4	45.000004	16
ACCGGGA	20	7.028358E-4	45.000004	4
CAAAAGT	20	7.028358E-4	45.000004	42
CGACGGT	20	7.028358E-4	45.000004	28
ACCATGC	20	7.028358E-4	45.000004	23
TTTCGTC	20	7.028358E-4	45.000004	15
CGTTGTG	20	7.028358E-4	45.000004	18
GACAATT	20	7.028358E-4	45.000004	25
TAGGTAA	20	7.028358E-4	45.000004	41
CGCATGT	20	7.028358E-4	45.000004	38
GACGTAA	20	7.028358E-4	45.000004	25
ATCAGGC	20	7.028358E-4	45.000004	17
GAACTAA	20	7.028358E-4	45.000004	24
TGGAAGT	20	7.028358E-4	45.000004	25
GGACTAA	20	7.028358E-4	45.000004	8
GGGTACG	20	7.028358E-4	45.000004	7
>>END_MODULE