FastQCFastQC Report
Sat 18 Jun 2016
SRR3548254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695369
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC303434.363582500801733No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG265713.8211366914544653No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC239833.4489601923583018No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC81161.1671501030388183No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT75641.0877677894758035No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTC60040.8634264685368489No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT52270.7516872336845617No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52140.7498177226767372No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGCTATT40160.5775350928787449No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTA36050.5184297833236742No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC24120.34686619622099923No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGT15750.2264984490249062No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT15160.21801374522016367No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTAT13450.19342248504031673No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGCAGCTATT9840.1415076024384176No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT9360.13460479256337282No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATG8710.12525723752424972No Hit
CCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC7590.10915068114914526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGA302.164259E-645.00000415
TTGTGCA302.164259E-645.00000432
CAAGTAA302.164259E-645.00000413
GTCTCAC850.045.00000422
ACCGAGT302.164259E-645.00000430
GTCTAGC302.164259E-645.00000433
ATGAGTT302.164259E-645.00000428
CTAAACT302.164259E-645.00000444
ACGTGTG302.164259E-645.0000043
TTTATCG302.164259E-645.00000423
CTACATC302.164259E-645.00000445
CGTAAGA302.164259E-645.00000414
CCAACCG302.164259E-645.0000044
TGAGCGA302.164259E-645.00000414
CTGATAG600.045.0000041
TATCGCC302.164259E-645.00000425
TTCGAAA302.164259E-645.00000437
TAATGTA302.164259E-645.00000414
GCGTAAG650.045.0000041
CTAATGT302.164259E-645.00000413
Produced by FastQC (version 0.11.5)