##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548254_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695369
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.830721530583045	28.0	25.0	31.0	16.0	33.0
2	27.742788361287317	30.0	26.0	31.0	16.0	33.0
3	27.709211943586787	30.0	26.0	31.0	16.0	33.0
4	30.803062834264974	33.0	28.0	35.0	19.0	37.0
5	32.02530598861899	33.0	32.0	35.0	26.0	37.0
6	31.34299487034941	35.0	30.0	35.0	18.0	37.0
7	32.0466629947553	35.0	31.0	35.0	26.0	35.0
8	32.849639543896835	35.0	32.0	35.0	28.0	37.0
9	34.17285786395425	35.0	33.0	37.0	28.0	39.0
10	33.69042623412893	35.0	32.0	37.0	27.0	39.0
11	33.84612342511674	35.0	32.0	39.0	27.0	39.0
12	33.31812462160378	35.0	31.0	37.0	25.0	39.0
13	33.256620585617135	35.0	31.0	38.0	24.0	39.0
14	34.15021377139332	36.0	32.0	39.0	24.0	40.0
15	34.830974633611795	37.0	32.0	39.0	27.0	40.0
16	35.077230937818626	36.0	33.0	39.0	27.0	41.0
17	34.4837690492386	36.0	32.0	39.0	27.0	40.0
18	34.39742496429953	36.0	32.0	39.0	27.0	40.0
19	33.7263150931376	36.0	32.0	38.0	25.0	40.0
20	33.29328313456596	35.0	31.0	38.0	25.0	40.0
21	34.23551093016801	35.0	33.0	38.0	27.0	40.0
22	34.56133793712403	36.0	33.0	39.0	27.0	40.0
23	34.79394105863218	35.0	33.0	39.0	28.0	40.0
24	34.61603120070063	36.0	33.0	39.0	27.0	40.0
25	33.92255622554356	35.0	33.0	39.0	24.0	40.0
26	33.83599355162511	35.0	32.0	39.0	24.0	40.0
27	34.1741391980373	35.0	33.0	39.0	25.0	40.0
28	33.979294446545644	35.0	33.0	39.0	24.0	40.0
29	33.91688873101907	35.0	33.0	39.0	24.0	40.0
30	33.606578665427996	35.0	32.0	39.0	23.0	40.0
31	33.48827169459668	35.0	32.0	39.0	23.0	40.0
32	33.084553668627734	35.0	32.0	39.0	20.0	40.0
33	32.882800354919475	35.0	31.0	39.0	18.0	40.0
34	32.75290097775426	35.0	32.0	39.0	15.0	40.0
35	32.602743291691176	36.0	31.0	39.0	15.0	40.0
36	32.386840655824464	36.0	31.0	39.0	12.0	40.0
37	32.39219752390458	36.0	31.0	40.0	12.0	40.0
38	32.078408729753555	35.0	31.0	39.0	10.0	40.0
39	32.04552546921131	35.0	31.0	39.0	10.0	40.0
40	31.830872529549058	35.0	30.0	39.0	10.0	40.0
41	31.40744554330147	35.0	30.0	39.0	10.0	40.0
42	31.658318101612238	35.0	30.0	39.0	10.0	40.0
43	31.63454079776349	35.0	30.0	39.0	10.0	40.0
44	31.757702744873583	35.0	30.0	39.0	10.0	40.0
45	31.675227109635316	35.0	30.0	39.0	10.0	40.0
46	31.413222332315648	35.0	30.0	39.0	10.0	40.0
47	31.47483134853581	35.0	30.0	39.0	10.0	40.0
48	31.368077955732854	35.0	30.0	38.0	10.0	40.0
49	31.522079644045103	35.0	30.0	39.0	10.0	40.0
50	31.31272748713273	35.0	29.0	38.0	10.0	40.0
51	28.989251749790398	33.0	24.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	28.0
10	29.0
11	33.0
12	29.0
13	54.0
14	90.0
15	218.0
16	531.0
17	1136.0
18	2272.0
19	3769.0
20	5479.0
21	7532.0
22	10094.0
23	12884.0
24	16211.0
25	19967.0
26	22232.0
27	22578.0
28	23780.0
29	26380.0
30	32548.0
31	41172.0
32	52344.0
33	64019.0
34	70987.0
35	69248.0
36	76300.0
37	71107.0
38	38961.0
39	3343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53932947830576	16.682078148436297	19.060815193084537	20.717777180173407
2	30.90920072652074	37.03314355399795	21.361751818099457	10.695903901381856
3	21.092110807355517	36.23543758781309	32.73916438610292	9.933287218728474
4	20.41966207869491	19.910436041871293	47.745729245911164	11.924172633522634
5	30.21000360959433	23.003613908586665	31.984313364558957	14.802069117260045
6	16.166812152972017	42.66152215586257	31.700866734064938	9.470798957100476
7	60.188763088374664	5.204431028705622	32.06355186958291	2.543254013336804
8	56.31053440691202	16.826893347273174	20.63968914346196	6.222883102352852
9	53.524675388175204	4.38486616458312	20.4853825810469	21.605075866194785
10	41.27132500873637	17.675219919208363	27.324916698903746	13.728538373151522
11	35.3576302653699	18.77851903090302	30.895251298231585	14.9685994054955
12	29.480175273847408	13.818850135683356	35.83449938090424	20.866475209564996
13	19.311904902289285	26.162943703271214	42.09923076812455	12.42592062631495
14	15.804270825993106	34.14144145051045	31.257648816671434	18.79663890682501
15	10.47947205009139	19.241870143765397	55.80576643479937	14.472891371343849
16	11.76612704909192	19.790787337370517	40.547536631630116	27.895548981907446
17	12.037206145226492	19.76188182101877	40.52970437278625	27.671207660968495
18	14.343607494725822	21.880037792884067	39.747385920281175	24.028968792108937
19	18.25131692669647	24.92518360755225	33.607480344967925	23.216019120783354
20	20.850224844650825	21.233043175637683	41.9948257687645	15.921906210946993
21	17.810256137388926	30.303622968524625	36.030078994030504	15.856041900055942
22	15.246149885887924	20.22753387050616	36.45201324764262	28.074302995963297
23	15.024253310113048	31.470485454485313	34.34838193822273	19.156879297178907
24	18.958567321810435	20.030084746372072	36.19215121755499	24.8191967142625
25	13.454151680618493	31.765292959565354	33.854399606539836	20.92615575327632
26	15.803407974758727	22.66652669302198	36.445398054845704	25.084667277373597
27	19.299537367929833	28.654139025467057	29.902397144537645	22.143926462065462
28	14.171181056388765	25.797094779893843	42.88183683770775	17.149887326009644
29	24.60463437397986	18.341772497767373	33.70383206614042	23.349761062112346
30	15.439284753850114	26.812383065681672	34.87198882895269	22.876343351515526
31	24.73578776160571	25.640487280853762	27.390061967099484	22.233662990441047
32	29.611328661473262	27.25171815252046	26.106139330341154	17.030813855665123
33	18.824969189020504	22.07374789500251	27.104170591441378	31.997112324535603
34	25.750500813237288	24.62519899506593	31.601207416493978	18.023092775202805
35	17.18281948145517	23.725964200302286	28.43037293868435	30.660843379558187
36	27.261209516098646	23.101259906610736	29.725081215872436	19.912449361418183
37	21.990626559423845	22.168805339323438	33.13693880515237	22.703629296100345
38	18.774492391809243	30.48395887651017	23.908601044912846	26.832947686767746
39	27.17463677558246	21.27273433241919	33.52809803140491	18.02453086059344
40	19.916763617590085	24.3818749469706	29.976314733616256	25.72504670182306
41	15.939882278329923	22.870015775796734	36.92571857531757	24.264383370555777
42	21.107929746652495	25.884961797261596	32.677039097227514	20.330069358858392
43	23.74408407622428	20.54175552835976	31.66548983345533	24.048670561960627
44	20.293829607014406	22.91042597527356	32.80186490913458	23.99387950857746
45	18.03330318147631	24.286961311188733	29.57609556940272	28.103639937932236
46	28.40664452973889	26.724516048313916	26.54533060864088	18.323508813306315
47	16.482184279138128	21.10836117226969	40.950488158085854	21.458966390506333
48	21.554886686061643	23.685697809364527	29.662380692840777	25.09703481173305
49	17.98800349167133	18.157122333609927	40.33153620595684	23.523337968761908
50	22.441753946465834	20.844041077471097	32.81940955090031	23.894795425162755
51	19.031478251115594	22.08884779160417	26.392318323077387	32.48735563420285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105.0
1	211.0
2	317.0
3	6757.5
4	13198.0
5	11139.0
6	9080.0
7	9114.5
8	9149.0
9	9534.5
10	9920.0
11	9740.0
12	9560.0
13	8989.0
14	8418.0
15	7927.0
16	7436.0
17	7057.0
18	6678.0
19	6301.0
20	5924.0
21	5450.5
22	4977.0
23	4769.0
24	4561.0
25	4513.0
26	4801.5
27	5138.0
28	4871.0
29	4604.0
30	5209.5
31	5815.0
32	6715.0
33	7615.0
34	8174.0
35	8733.0
36	9181.5
37	9630.0
38	11948.5
39	14267.0
40	19825.0
41	25383.0
42	30803.5
43	36224.0
44	43637.0
45	51050.0
46	107100.0
47	163150.0
48	115936.0
49	68722.0
50	62117.0
51	55512.0
52	44312.0
53	33112.0
54	29908.5
55	26705.0
56	24810.0
57	22915.0
58	20507.5
59	18100.0
60	16606.0
61	15112.0
62	13676.0
63	12240.0
64	9389.0
65	6538.0
66	5488.5
67	4439.0
68	3419.0
69	2399.0
70	1966.0
71	1533.0
72	1446.0
73	1359.0
74	1014.5
75	458.5
76	247.0
77	218.5
78	190.0
79	145.5
80	101.0
81	81.0
82	61.0
83	36.5
84	12.0
85	7.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695369.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.72502926603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.95142511278823	24.95458568402556
2	7.750625777016615	4.607751561037308
3	2.5774857557607134	2.29847518568288
4	1.2914630804355476	1.5355511144777565
5	0.6683630348763735	0.9933555386016417
6	0.46780061983376275	0.8343232269135343
7	0.31269544004032634	0.650641677458698
8	0.21141607779786323	0.5027479279880609
9	0.17520453751165435	0.46871640045676594
>10	1.5268508175529711	10.31306454821278
>50	0.5811919339697692	12.706180555440227
>100	0.4741302619226993	20.491502588751416
>500	0.004440345845269817	0.9459241064472369
>1k	0.0029602305635132112	2.1219497513465226
>5k	0.002466858802927676	4.7112886697081375
>10k+	0.0014801152817566056	11.863941463451491
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC	30343	4.363582500801733	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG	26571	3.8211366914544653	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	23983	3.4489601923583018	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	8116	1.1671501030388183	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	7564	1.0877677894758035	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTC	6004	0.8634264685368489	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	5227	0.7516872336845617	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5214	0.7498177226767372	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGCTATT	4016	0.5775350928787449	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTA	3605	0.5184297833236742	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2412	0.34686619622099923	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGT	1575	0.2264984490249062	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	1516	0.21801374522016367	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTAT	1345	0.19342248504031673	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGCAGCTATT	984	0.1415076024384176	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	936	0.13460479256337282	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATG	871	0.12525723752424972	No Hit
CCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	759	0.10915068114914526	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.438085390634325E-4	0.0	0.0	0.25698585930635387	0.0
2	1.438085390634325E-4	0.0	0.0	1.879577605559063	0.0
3	1.438085390634325E-4	0.0	0.0	2.0919828177557527	0.0
4	1.438085390634325E-4	0.0	0.0	3.532225336476029	0.0
5	1.438085390634325E-4	0.0	0.0	8.020202223567631	0.0
6	1.438085390634325E-4	0.0	0.0	8.60924199957145	0.0
7	1.438085390634325E-4	0.0	0.0	9.813638514227698	0.0
8	1.438085390634325E-4	0.0	0.0	11.726435892310414	0.0
9	1.438085390634325E-4	0.0	0.0	12.247310420798167	0.0
10	1.438085390634325E-4	0.0	0.0	17.229557256650786	0.0
11	1.438085390634325E-4	0.0	0.0	18.00482909074175	0.0
12	1.438085390634325E-4	0.0	0.0	23.799737980841826	0.0
13	1.438085390634325E-4	0.0	0.0	24.781375068488817	0.0
14	1.438085390634325E-4	0.0	0.0	25.244294755734007	0.0
15	1.438085390634325E-4	0.0	0.0	26.092621327669196	0.0
16	2.87617078126865E-4	0.0	0.0	26.881554972971184	0.0
17	2.87617078126865E-4	0.0	0.0	27.32793667822408	0.0
18	8.628512343805951E-4	0.0	0.0	28.203011638425068	0.0
19	8.628512343805951E-4	0.0	0.0	29.168973595314142	0.0
20	8.628512343805951E-4	0.0	0.0	29.603419191824774	0.0
21	0.0010066597734440276	0.0	0.0	29.830205257927805	0.0
22	0.0010066597734440276	0.0	0.0	30.10272243945301	0.0
23	0.0010066597734440276	0.0	0.0	30.34992931810305	0.0
24	0.0010066597734440276	0.0	0.0	30.515021520947872	0.0
25	0.0010066597734440276	0.0	0.0	30.653221526987828	0.0
26	0.0010066597734440276	0.0	0.0	30.790558681793406	0.0
27	0.0010066597734440276	0.0	0.0	30.9805297618962	0.0
28	0.0010066597734440276	0.0	0.0	31.12160593871743	0.0
29	0.0012942768515708926	0.0	0.0	31.25894309352301	0.0
30	0.0012942768515708926	0.0	0.0	31.428637169617858	0.0
31	0.0012942768515708926	0.0	0.0	31.58495705157981	0.0
32	0.0012942768515708926	0.0	0.0	31.72574561132291	0.0
33	0.0012942768515708926	0.0	0.0	31.871423661394168	0.0
34	0.0012942768515708926	0.0	0.0	32.02242262741077	0.0
35	0.0012942768515708926	0.0	0.0	32.18061202038054	0.0
36	0.0012942768515708926	0.0	0.0	32.31277206777984	0.0
37	0.0012942768515708926	0.0	0.0	32.46031962885892	0.0
38	0.0012942768515708926	0.0	0.0	32.59434918726604	0.0
39	0.0012942768515708926	0.0	0.0	32.7309672993763	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGA	30	2.164259E-6	45.000004	15
TTGTGCA	30	2.164259E-6	45.000004	32
CAAGTAA	30	2.164259E-6	45.000004	13
GTCTCAC	85	0.0	45.000004	22
ACCGAGT	30	2.164259E-6	45.000004	30
GTCTAGC	30	2.164259E-6	45.000004	33
ATGAGTT	30	2.164259E-6	45.000004	28
CTAAACT	30	2.164259E-6	45.000004	44
ACGTGTG	30	2.164259E-6	45.000004	3
TTTATCG	30	2.164259E-6	45.000004	23
CTACATC	30	2.164259E-6	45.000004	45
CGTAAGA	30	2.164259E-6	45.000004	14
CCAACCG	30	2.164259E-6	45.000004	4
TGAGCGA	30	2.164259E-6	45.000004	14
CTGATAG	60	0.0	45.000004	1
TATCGCC	30	2.164259E-6	45.000004	25
TTCGAAA	30	2.164259E-6	45.000004	37
TAATGTA	30	2.164259E-6	45.000004	14
GCGTAAG	65	0.0	45.000004	1
CTAATGT	30	2.164259E-6	45.000004	13
>>END_MODULE