FastQCFastQC Report
Sat 18 Jun 2016
SRR3548252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700092
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC311124.443987361661039No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTC274403.919484867703102No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCG264143.772932700273678No Hit
GAACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT86831.2402655650971586No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC78621.122995263479657No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTC64460.9207361318226748No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58950.8420321900550214No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCT55110.7871822560463482No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGGATAATCGTA44040.6290601806619701No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGGATAAT41710.5957788404952492No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTT29710.42437279671814565No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGGATAATCGT17850.2549664901184416No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT15880.22682733126503374No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTAT14310.204401707204196No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATG9380.1339823908857693No Hit
TTGTTGGGGACTCTTAAAATGTAGTTTCAGTTTGTAAAGACCAAATTCCAA8280.11827017020620147No Hit
TTGATGCGGGATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTAT7990.11412785748158814No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCCCGGATAAT7880.11255663541363135No Hit
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC7730.11041405986641756No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTC253.8892365E-545.00000418
AGTCCGT302.1642809E-645.00000429
GTACCGG253.8892365E-545.0000042
AGTCATA253.8892365E-545.00000442
CTCGTAG253.8892365E-545.0000041
CGAGTAC351.2110104E-745.0000049
ACTGAGT351.2110104E-745.00000413
TCATTAC253.8892365E-545.00000441
GCGCGCC253.8892365E-545.00000416
CATTGTC302.1642809E-645.00000414
TTTCGAG253.8892365E-545.0000041
CGGTTGA253.8892365E-545.00000445
CACATAG302.1642809E-645.00000426
CGCATCG302.1642809E-645.00000421
TGTGGCC253.8892365E-545.00000415
AGGACGG600.045.0000042
GTCTATC253.8892365E-545.00000433
ATAGAGT302.1642809E-645.00000421
CCTGTTA253.8892365E-545.00000430
GTCTAAT253.8892365E-545.00000416
Produced by FastQC (version 0.11.5)