##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548252_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	700092
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.83691000611348	28.0	25.0	31.0	16.0	33.0
2	27.745560583466172	30.0	26.0	31.0	16.0	33.0
3	27.615320557869538	30.0	26.0	31.0	16.0	33.0
4	30.61659467612828	33.0	28.0	35.0	19.0	37.0
5	31.9054138598927	33.0	32.0	35.0	26.0	37.0
6	31.27215423115819	35.0	30.0	35.0	18.0	36.0
7	32.085397347777146	35.0	32.0	35.0	26.0	35.0
8	32.74489924181393	35.0	32.0	35.0	28.0	37.0
9	34.06031921518886	35.0	32.0	37.0	27.0	39.0
10	33.643396867840224	35.0	32.0	37.0	27.0	39.0
11	33.86975283248487	35.0	32.0	39.0	27.0	39.0
12	33.40355981785251	35.0	31.0	37.0	25.0	39.0
13	33.3030630260023	35.0	32.0	38.0	25.0	39.0
14	34.17483987818744	36.0	32.0	39.0	25.0	40.0
15	34.81941801934603	37.0	32.0	39.0	27.0	41.0
16	35.092866366134736	36.0	33.0	39.0	27.0	41.0
17	34.425959731006785	36.0	32.0	39.0	27.0	40.0
18	34.34503893773961	36.0	32.0	39.0	27.0	40.0
19	33.79667386572051	36.0	32.0	38.0	25.0	40.0
20	33.14239271410043	35.0	31.0	38.0	25.0	40.0
21	34.07822686161247	35.0	32.0	38.0	27.0	40.0
22	34.40276849328374	35.0	33.0	39.0	27.0	40.0
23	34.64064151568651	35.0	33.0	39.0	27.0	40.0
24	34.47679162167258	35.0	33.0	39.0	27.0	40.0
25	33.84808425178405	35.0	32.0	39.0	24.0	40.0
26	33.57821971969398	35.0	32.0	38.0	24.0	40.0
27	34.056741114025016	35.0	33.0	39.0	25.0	40.0
28	33.692754666529545	35.0	32.0	39.0	24.0	40.0
29	33.58846837272816	35.0	32.0	39.0	23.0	40.0
30	33.142698388211834	35.0	32.0	39.0	21.0	40.0
31	33.07964953177582	35.0	32.0	39.0	21.0	40.0
32	32.55887226250264	35.0	31.0	38.0	18.0	40.0
33	32.10833718996932	35.0	30.0	39.0	15.0	40.0
34	32.010831433583014	35.0	31.0	39.0	15.0	40.0
35	32.03709655302446	35.0	31.0	39.0	12.0	40.0
36	31.738455802951613	35.0	30.0	39.0	10.0	40.0
37	31.843116333281912	35.0	30.0	39.0	10.0	40.0
38	31.327018449003845	35.0	30.0	39.0	10.0	40.0
39	31.36682750267108	35.0	30.0	39.0	10.0	40.0
40	30.90627660364638	35.0	28.0	38.0	10.0	40.0
41	30.752659650445942	35.0	28.0	38.0	9.0	40.0
42	31.039100575352954	35.0	29.0	38.0	10.0	40.0
43	31.172604457699844	35.0	29.0	39.0	10.0	40.0
44	31.262298383641006	35.0	30.0	39.0	10.0	40.0
45	31.208404038326393	35.0	29.0	39.0	10.0	40.0
46	30.9541674522777	35.0	29.0	38.0	10.0	40.0
47	30.800024853876348	35.0	28.0	38.0	10.0	40.0
48	30.837122835284504	35.0	28.0	38.0	10.0	40.0
49	30.926665352553666	35.0	29.0	38.0	10.0	40.0
50	30.80129897213509	35.0	28.0	38.0	8.0	40.0
51	28.497651737200254	33.0	23.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	43.0
10	35.0
11	37.0
12	35.0
13	49.0
14	116.0
15	259.0
16	562.0
17	1241.0
18	2470.0
19	4207.0
20	6281.0
21	8818.0
22	11369.0
23	14620.0
24	18201.0
25	21732.0
26	23797.0
27	23866.0
28	24629.0
29	27905.0
30	34316.0
31	43476.0
32	54269.0
33	65182.0
34	71629.0
35	68598.0
36	72061.0
37	64169.0
38	32912.0
39	3201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59841278003462	15.314415819635133	19.204190306416873	21.88298109391337
2	30.697822571890555	38.012432651708636	20.350182547436624	10.939562228964194
3	20.5193031772967	37.84731149620335	32.40017026333682	9.233215063163128
4	18.29530975928878	19.285608177210996	50.04527976323112	12.373802300269107
5	31.237180256309173	22.094096204498836	31.589562514640935	15.079161024551057
6	15.081589276837901	41.93748821583449	33.31990652657079	9.661015980756815
7	58.77256132051216	3.8143558275198117	34.44547288070711	2.9676099712609196
8	54.257583289053436	17.160316072744724	22.026819332316325	6.5552813058855115
9	51.30140038737766	4.415705364437817	22.239648503339563	22.043245744844963
10	39.50723619181479	17.73281225896025	28.272427052444538	14.487524496780424
11	34.36805448426778	18.592842083611867	32.37503071024951	14.664072721870841
12	27.225850316815503	14.764345257480446	37.25667483702142	20.753129588682633
13	19.06506573421779	25.154265439399392	43.71482605143324	12.065842774949578
14	14.857047359489894	35.04225158979106	32.44587854167738	17.65482250904167
15	9.654588254115174	18.99521777137862	57.04864503522394	14.301548939282267
16	10.476337395656572	21.266490689795056	40.91019466013039	27.346977254417993
17	10.695879970061076	21.73785731018209	40.614519234614875	26.951743485141954
18	14.156853670660427	22.098952709072524	40.342126463379095	23.402067156887952
19	16.962770607291613	25.10112956582849	35.173805728389986	22.762294098489914
20	20.141781365877627	21.802705930077764	42.523839723921995	15.531672980122613
21	16.330139467384285	30.733246487604486	36.66975197545466	16.266862069556574
22	14.317689675071277	18.78267427709501	39.29812081840672	27.601515229426987
23	14.317689675071277	31.223610611176817	35.29507550436228	19.163624209389624
24	18.23088965450255	20.05222170800409	37.2935271364335	24.423361501059862
25	12.406512286956572	31.944801540368978	35.03653805499848	20.612148117675964
26	12.984293492855226	23.196379904355428	36.99785171091799	26.821474891871354
27	18.253886632042647	28.506824817309724	30.66596961542197	22.573318935225657
28	12.780320300760472	23.881004210875144	43.3265913622781	20.012084126086286
29	17.813087422795864	18.757963239117146	34.96026236551767	28.46868697256932
30	15.612662335807295	27.137290527530666	35.457339892471275	21.792707244190765
31	28.50139695925678	20.732418025059562	28.28842494986373	22.477760065819922
32	28.23128960193803	28.860064105860374	26.472806431154762	16.435839861046833
33	19.409591882209767	27.225421801706062	26.896322197654023	26.46866411843015
34	22.900133125360668	23.04997057529582	32.413025716620105	21.636870582723414
35	16.274289664786913	27.564377253275286	27.668220748130246	28.49311233380756
36	24.703324705895795	26.604217731383876	26.432811687606772	22.259645875113556
37	20.12678333704713	23.881147049244955	33.75384949406649	22.23822011964142
38	22.991406843671975	30.425286962284957	22.900704478839923	23.682601715203145
39	27.077726927318125	23.196951257834687	31.65198288224976	18.07333893259743
40	19.740405546699577	30.100043994217902	30.160036109539888	19.999514349542633
41	15.404403992618112	29.028756220611008	32.146489318546706	23.42035046822418
42	20.52044588425521	26.348679887786176	32.91967341435126	20.211200813607356
43	22.99483496454752	27.669934808568016	25.3748078823926	23.960422344491867
44	20.315901338681204	23.756449152397114	31.861241094027644	24.066408414894042
45	17.41399701753484	24.389080292304442	28.80092902075727	29.39599366940345
46	28.451260691451978	27.49010130097187	26.350536786593764	17.708101220982385
47	16.392559834993115	22.24436216954343	40.519817395428035	20.843260600035425
48	21.49903155585266	24.117116036178103	29.333001948315363	25.050850459653873
49	18.34330345154637	20.097644309605023	39.18513566788365	22.37391657096496
50	21.09565599949721	22.476188843751967	32.32832256332025	24.099832593430577
51	18.31616416128166	22.13209121086943	27.498528764790915	32.053215863058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	95.0
1	221.0
2	347.0
3	7106.5
4	13866.0
5	11710.5
6	9555.0
7	9779.0
8	10003.0
9	10454.0
10	10905.0
11	10809.5
12	10714.0
13	10282.0
14	9850.0
15	9303.0
16	8756.0
17	8221.5
18	7687.0
19	6878.0
20	6069.0
21	5701.0
22	5333.0
23	5187.5
24	5042.0
25	4684.0
26	4719.0
27	5112.0
28	5342.5
29	5573.0
30	7011.0
31	8449.0
32	9482.0
33	10515.0
34	10939.0
35	11363.0
36	11801.5
37	12240.0
38	13952.5
39	15665.0
40	20991.0
41	26317.0
42	32636.0
43	38955.0
44	43672.5
45	48390.0
46	107794.0
47	167198.0
48	115995.0
49	64792.0
50	58555.5
51	52319.0
52	42790.5
53	33262.0
54	28687.0
55	24112.0
56	21740.5
57	19369.0
58	17857.5
59	16346.0
60	14497.0
61	12648.0
62	11147.5
63	9647.0
64	8098.5
65	6550.0
66	4774.5
67	2999.0
68	2611.0
69	2223.0
70	1788.0
71	1353.0
72	1090.5
73	828.0
74	708.5
75	459.5
76	330.0
77	264.0
78	198.0
79	162.5
80	127.0
81	83.0
82	39.0
83	29.0
84	19.0
85	14.0
86	9.0
87	5.5
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	700092.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.366949796829992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.64433589228473	26.5503463451931
2	7.632124325564495	4.787929211262929
3	2.4604370012033643	2.3152921168502654
4	1.1209799346587002	1.4064688533477283
5	0.6620462517250433	1.038318577051945
6	0.41969077860587256	0.7898651749633736
7	0.294888784213121	0.6474833183042839
8	0.21964002365565122	0.551155007630509
9	0.1564385353524792	0.44162997162217454
>10	1.4383785272622938	10.252511254523624
>50	0.5295932541660396	12.106322366294469
>100	0.41123542786242734	18.51937999326026
>500	0.003713186707561421	0.8349539260972427
>1k	0.0027848900306710654	2.380383032552732
>5k	0.002320741692225888	5.007830897291519
>10k+	0.0013924450153355327	12.37012995375385
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC	31112	4.443987361661039	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTC	27440	3.919484867703102	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCG	26414	3.772932700273678	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT	8683	1.2402655650971586	No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	7862	1.122995263479657	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTC	6446	0.9207361318226748	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5895	0.8420321900550214	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCT	5511	0.7871822560463482	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGGATAATCGTA	4404	0.6290601806619701	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGGATAAT	4171	0.5957788404952492	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTT	2971	0.42437279671814565	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGGATAATCGT	1785	0.2549664901184416	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	1588	0.22682733126503374	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTAT	1431	0.204401707204196	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATG	938	0.1339823908857693	No Hit
TTGTTGGGGACTCTTAAAATGTAGTTTCAGTTTGTAAAGACCAAATTCCAA	828	0.11827017020620147	No Hit
TTGATGCGGGATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTAT	799	0.11412785748158814	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCCCGGATAAT	788	0.11255663541363135	No Hit
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	773	0.11041405986641756	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4283836981425298E-4	0.0	0.0	0.26753626666209585	0.0
2	1.4283836981425298E-4	0.0	0.0	1.8561846157362176	0.0
3	1.4283836981425298E-4	0.0	0.0	2.099295521160076	0.0
4	1.4283836981425298E-4	0.0	0.0	3.7855024768173324	0.0
5	1.4283836981425298E-4	0.0	0.0	8.870976957314182	0.0
6	1.4283836981425298E-4	0.0	0.0	9.80928220862401	0.0
7	1.4283836981425298E-4	0.0	0.0	11.067116893208322	0.0
8	1.4283836981425298E-4	0.0	0.0	12.957011364220703	0.0
9	1.4283836981425298E-4	0.0	0.0	13.402667078041171	0.0
10	1.4283836981425298E-4	0.0	0.0	18.26817046902407	0.0
11	1.4283836981425298E-4	0.0	0.0	19.039640504390853	0.0
12	1.4283836981425298E-4	0.0	0.0	24.868588699770886	0.0
13	1.4283836981425298E-4	0.0	0.0	25.887883306765396	0.0
14	1.4283836981425298E-4	0.0	0.0	26.38838895459454	0.0
15	1.4283836981425298E-4	0.0	0.0	27.350119698553904	0.0
16	1.4283836981425298E-4	0.0	0.0	28.0575981442439	0.0
17	1.4283836981425298E-4	0.0	0.0	28.498683030230314	0.0
18	7.141918490712649E-4	0.0	0.0	29.276866468978362	0.0
19	7.141918490712649E-4	0.0	0.0	30.227455820092217	0.0
20	7.141918490712649E-4	0.0	0.0	30.64668643549705	0.0
21	8.570302188855179E-4	0.0	0.0	30.90393833953252	0.0
22	8.570302188855179E-4	0.0	0.0	31.216183015946477	0.0
23	8.570302188855179E-4	0.0	0.0	31.45943675974015	0.0
24	8.570302188855179E-4	0.0	0.0	31.647412054415707	0.0
25	8.570302188855179E-4	0.0	0.0	31.797535181090485	0.0
26	8.570302188855179E-4	0.0	0.0	31.9880815664227	0.0
27	8.570302188855179E-4	0.0	0.0	32.18805528416265	0.0
28	8.570302188855179E-4	0.0	0.0	32.33346474463356	0.0
29	8.570302188855179E-4	0.0	0.0	32.478302851625216	0.0
30	8.570302188855179E-4	0.0	0.0	32.66584963119133	0.0
31	8.570302188855179E-4	0.0	0.0	32.83011375647772	0.0
32	8.570302188855179E-4	0.0	0.0	32.98352216565823	0.0
33	8.570302188855179E-4	0.0	0.0	33.153071310627745	0.0
34	8.570302188855179E-4	0.0	0.0	33.337761322797576	0.0
35	8.570302188855179E-4	0.0	0.0	33.50488221548025	0.0
36	8.570302188855179E-4	0.0	0.0	33.66057603857779	0.0
37	8.570302188855179E-4	0.0	0.0	33.818412437222534	0.0
38	8.570302188855179E-4	0.0	0.0	33.9553944338744	0.0
39	8.570302188855179E-4	0.0	0.0	34.12037275100987	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTC	25	3.8892365E-5	45.000004	18
AGTCCGT	30	2.1642809E-6	45.000004	29
GTACCGG	25	3.8892365E-5	45.000004	2
AGTCATA	25	3.8892365E-5	45.000004	42
CTCGTAG	25	3.8892365E-5	45.000004	1
CGAGTAC	35	1.2110104E-7	45.000004	9
ACTGAGT	35	1.2110104E-7	45.000004	13
TCATTAC	25	3.8892365E-5	45.000004	41
GCGCGCC	25	3.8892365E-5	45.000004	16
CATTGTC	30	2.1642809E-6	45.000004	14
TTTCGAG	25	3.8892365E-5	45.000004	1
CGGTTGA	25	3.8892365E-5	45.000004	45
CACATAG	30	2.1642809E-6	45.000004	26
CGCATCG	30	2.1642809E-6	45.000004	21
TGTGGCC	25	3.8892365E-5	45.000004	15
AGGACGG	60	0.0	45.000004	2
GTCTATC	25	3.8892365E-5	45.000004	33
ATAGAGT	30	2.1642809E-6	45.000004	21
CCTGTTA	25	3.8892365E-5	45.000004	30
GTCTAAT	25	3.8892365E-5	45.000004	16
>>END_MODULE