SRR3548251_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504650
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

$Per Sequence quality graph$

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTC	16126	3.195482017239671	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC	14748	2.9224214802338255	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCG	14072	2.7884672545328444	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6133	1.215297731100763	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT	5323	1.054790448825919	No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	5263	1.0429010205092637	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTC	4386	0.8691172099474884	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGGATAAT	4148	0.8219558109580898	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCT	3588	0.7109878133359755	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGGATAATCGTA	3051	0.6045774299019122	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTT	3030	0.6004161299910828	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	2305	0.45675220449816706	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC	1407	0.27880709402556225	No Hit
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	1363	0.2700881799266819	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGGATAATCGT	1231	0.24393143763004063	No Hit
TCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	1104	0.21876548102645396	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCG	949	0.18805112454176162	No Hit
ACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	828	0.1640741107698405	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTAT	773	0.15317546814623997	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATG	663	0.13137818289903894	No Hit
TTGATGCGGGATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTAT	594	0.11770534033488556	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCGGATAA	568	0.11255325473100168	No Hit
TTGTTGGGGACTCTTAAAATGTAGTTTCAGTTTGTAAAGACCAAATTCCAA	567	0.11235509759239078	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	533	0.10561775487961952	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.0295203E-4	45.000004	25
AGACTGC	20	7.0295203E-4	45.000004	13
CGGCGAA	40	6.8012014E-9	45.000004	31
CTCACGA	20	7.0295203E-4	45.000004	24
AGATCCG	20	7.0295203E-4	45.000004	17
CTGTACG	20	7.0295203E-4	45.000004	1
CGCGGTT	20	7.0295203E-4	45.000004	18
TAAAGCG	20	7.0295203E-4	45.000004	1
AACCCTA	20	7.0295203E-4	45.000004	18
AGCCCTT	20	7.0295203E-4	45.000004	17
TCCGAGT	20	7.0295203E-4	45.000004	15
TGGACCG	20	7.0295203E-4	45.000004	45
CGTGATT	20	7.0295203E-4	45.000004	23
GATCCAT	20	7.0295203E-4	45.000004	11
GCAAACA	40	6.8012014E-9	45.000004	33
TTCGTAC	40	6.8012014E-9	45.000004	33
GTTAGAA	20	7.0295203E-4	45.000004	9
TTACTAG	40	6.8012014E-9	45.000004	1
ATGTCGC	20	7.0295203E-4	45.000004	34
GTAGCAA	20	7.0295203E-4	45.000004	26

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content