##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504650
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.404973744179134	30.0	25.0	31.0	16.0	33.0
2	27.795392846527296	30.0	25.0	31.0	16.0	33.0
3	27.590205092638463	30.0	25.0	31.0	16.0	34.0
4	31.955357178242345	35.0	32.0	35.0	19.0	37.0
5	33.061967700386404	35.0	32.0	35.0	28.0	37.0
6	31.960537005845637	35.0	32.0	35.0	25.0	37.0
7	33.54581194887546	35.0	33.0	35.0	30.0	37.0
8	32.923130882790055	35.0	33.0	35.0	27.0	37.0
9	35.57520261567423	37.0	34.0	39.0	30.0	39.0
10	35.0696720499356	37.0	34.0	39.0	30.0	39.0
11	35.48384821163182	37.0	35.0	39.0	30.0	39.0
12	35.50673734271277	37.0	34.0	39.0	30.0	39.0
13	35.2330526107203	37.0	34.0	39.0	30.0	39.0
14	35.898628752600814	38.0	34.0	40.0	29.0	41.0
15	36.300126820568714	38.0	34.0	40.0	30.0	41.0
16	36.15234518973546	37.0	34.0	40.0	31.0	41.0
17	35.522451203804614	37.0	34.0	39.0	29.0	41.0
18	35.62489051818092	37.0	34.0	39.0	30.0	40.0
19	35.16836223124938	37.0	34.0	39.0	28.0	40.0
20	34.81567621123551	36.0	33.0	39.0	27.0	40.0
21	35.339710690577625	37.0	34.0	39.0	30.0	40.0
22	35.83078767462598	37.0	34.0	40.0	30.0	40.0
23	35.941464381254335	37.0	34.0	40.0	30.0	41.0
24	35.90467056375706	37.0	34.0	40.0	30.0	41.0
25	35.322706826513425	37.0	34.0	40.0	29.0	41.0
26	35.4088873476667	37.0	34.0	40.0	29.0	41.0
27	35.50327950064401	37.0	34.0	40.0	29.0	41.0
28	35.22466263747152	37.0	34.0	40.0	27.0	41.0
29	35.21008421678391	37.0	34.0	40.0	27.0	41.0
30	34.595535519667095	37.0	33.0	40.0	25.0	41.0
31	34.78942435351234	37.0	34.0	40.0	25.0	41.0
32	34.26624987615179	37.0	33.0	40.0	24.0	40.0
33	33.8512553254731	37.0	33.0	40.0	21.0	41.0
34	33.71947092043991	37.0	33.0	40.0	19.0	41.0
35	33.6144516001189	37.0	33.0	40.0	18.0	41.0
36	33.330383434063215	37.0	33.0	40.0	15.0	41.0
37	33.614320816407414	37.0	33.0	40.0	17.0	41.0
38	33.16291489150897	37.0	32.0	40.0	15.0	41.0
39	33.12432577033588	37.0	32.0	40.0	15.0	41.0
40	32.79505796096304	36.0	31.0	40.0	15.0	41.0
41	32.45193302288715	36.0	31.0	39.0	14.0	40.0
42	32.78776974140494	36.0	32.0	40.0	14.0	41.0
43	32.9199147924304	36.0	33.0	40.0	14.0	40.0
44	33.17054592291687	37.0	33.0	40.0	15.0	41.0
45	33.24009907856931	37.0	33.0	40.0	15.0	41.0
46	32.91573962151987	36.0	32.0	40.0	15.0	40.0
47	32.69414247498266	36.0	32.0	39.0	15.0	40.0
48	32.860998711978596	36.0	32.0	40.0	15.0	40.0
49	32.927942138115526	36.0	32.0	40.0	13.0	40.0
50	32.79528782324383	36.0	32.0	40.0	12.0	40.0
51	30.835254136530267	34.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	16.0
11	22.0
12	22.0
13	24.0
14	36.0
15	60.0
16	150.0
17	333.0
18	788.0
19	1697.0
20	3082.0
21	4397.0
22	5748.0
23	6548.0
24	7905.0
25	9880.0
26	11674.0
27	12220.0
28	11947.0
29	12766.0
30	15833.0
31	21207.0
32	29424.0
33	42163.0
34	53327.0
35	56151.0
36	69042.0
37	72430.0
38	47375.0
39	8377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.367977806400475	18.311106707619142	20.719706727434854	16.60120875854553
2	29.08431586247894	37.80838204696324	20.823739225205586	12.283562865352224
3	21.6569899930645	38.26870107995641	29.117011790349746	10.957297136629347
4	19.583275537501237	21.659764193005053	45.70771822054889	13.049242048944812
5	31.21291984543743	24.206479738432577	28.584365401763602	15.996235014366391
6	16.117903497473495	41.79887050430992	30.07351629842465	12.009709699791934
7	60.1906271673437	5.31814128603983	30.17655801050233	4.314673536114139
8	54.923412265926885	16.012285742593875	19.11522837610225	9.949073615376994
9	50.815416625383925	6.401466362825721	21.467749925691074	21.315367086099275
10	38.31705142177747	20.345982364014663	25.59873179431289	15.738234419894978
11	31.50757951055187	20.9238085802041	30.781729911820072	16.786881997423958
12	24.240364609135042	15.815912018230458	35.83374616070544	24.10997721192906
13	21.54701278113544	24.48568314673536	40.926582780144656	13.040721291984545
14	14.45932824730011	36.2631526800753	30.389775091647675	18.887743980976914
15	9.637966907757852	21.450510254631926	51.32705835727732	17.584464480332905
16	10.23124938075894	24.53918557416031	36.98761517883682	28.24194986624393
17	10.382245120380462	26.449618547508173	35.69384722084613	27.474289111265232
18	13.980976914693352	23.74259387694442	36.61309818686218	25.663331021500053
19	15.84444664619043	27.806796789854353	31.734865748538592	24.613890815416624
20	19.905677202021202	24.232240166451994	37.8438521747746	18.018230456752203
21	16.035866442088576	33.14633904686416	33.60507282274844	17.21272168829882
22	13.483206182502725	21.766174576439116	36.239968294857825	28.51065094620034
23	14.767264440701478	31.787179233131873	32.04795402754384	21.397602298622807
24	17.90230853066482	23.48181908253245	33.39780045576142	25.218071931041315
25	12.936094322797977	33.04290102050926	30.335876349945508	23.68512830674725
26	12.97057366491628	25.0325968493015	34.54968790250669	27.447141583275535
27	19.992866343010007	28.027147527989694	27.352026156742298	24.627959972258
28	14.4101852769246	25.997423957198055	37.038145249182605	22.554245516694742
29	17.85732686020014	20.305360150599423	31.49291588229466	30.344397106905774
30	17.416030912513623	28.15178836817596	30.19439215297731	24.2377885663331
31	26.483899732487863	22.940651936986033	24.529277717229764	26.046170613296344
32	27.77945110472605	29.549985138214602	22.681264242544337	19.98929951451501
33	20.223124938075895	28.107203011988506	24.29505597939166	27.37461607054394
34	22.503120974933122	24.065590012880214	29.683741206776975	23.747547805409692
35	17.784008718914098	27.985138214604184	25.331814128603984	28.899038937877737
36	22.37947092043991	29.38690181313782	24.773803626275637	23.459823640146638
37	20.98860596452987	24.216783909640345	31.794114732983253	23.000495392846528
38	23.879520459724564	30.236599623501437	21.435846626374715	24.448033290399287
39	27.277915386901814	24.362033092242147	28.69057762805905	19.669473892796987
40	21.02229267809373	28.508273060537004	29.525611810165458	20.943822451203804
41	16.67928267115823	28.244525909045876	29.328445457247597	25.7477459625483
42	22.30179332210443	25.754879619538297	30.19637372436342	21.746953333993858
43	23.539680967006838	26.149014168235414	25.208758545526607	25.102546319231152
44	21.379966313286435	22.510452789061727	29.54106806697711	26.568512830674724
45	19.628257207965916	21.2650351728921	27.87575547409095	31.230952145051027
46	28.559793916575842	25.51451501040325	25.371049242048944	20.554641830971963
47	16.449816704646786	22.08778361240464	38.10086198355296	23.361537699395623
48	20.26117110868919	22.886555038145246	29.457049440206085	27.39522441295948
49	19.65441395026256	18.82690973942336	37.32071732884177	24.197958981472308
50	20.097493312196573	20.423065490934313	33.569206380659864	25.910234816209254
51	17.727137620132766	19.81650648964629	28.104825126325178	34.351530763895774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	655.0
1	703.5
2	752.0
3	5151.5
4	9551.0
5	6643.5
6	3736.0
7	3850.0
8	3964.0
9	4177.0
10	4390.0
11	4475.5
12	4561.0
13	4476.5
14	4392.0
15	4270.5
16	4149.0
17	3920.5
18	3692.0
19	3534.0
20	3376.0
21	3257.5
22	3139.0
23	3120.0
24	3101.0
25	2995.0
26	3442.0
27	3995.0
28	4346.0
29	4697.0
30	5788.5
31	6880.0
32	7198.5
33	7517.0
34	7600.0
35	7683.0
36	7778.5
37	7874.0
38	9029.5
39	10185.0
40	13237.5
41	16290.0
42	20762.0
43	25234.0
44	29541.0
45	33848.0
46	71541.5
47	109235.0
48	87027.0
49	64819.0
50	57389.0
51	49959.0
52	40450.0
53	30941.0
54	25922.0
55	20903.0
56	18069.5
57	15236.0
58	13686.5
59	12137.0
60	10602.0
61	9067.0
62	7687.5
63	6308.0
64	5256.5
65	4205.0
66	3084.0
67	1963.0
68	1633.0
69	1303.0
70	1056.0
71	809.0
72	649.5
73	490.0
74	407.5
75	238.5
76	152.0
77	136.5
78	121.0
79	102.5
80	84.0
81	56.0
82	28.0
83	18.5
84	9.0
85	5.0
86	1.0
87	1.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.951066271516115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.12186619499685	25.28027990322375
2	9.179536742734014	5.865919736178233
3	3.607913874186338	3.4583008588815045
4	1.7809141963302855	2.276084300433313
5	1.0948454373217986	1.7490739562467918
6	0.7358661394664511	1.4107024673434378
7	0.5171712022097014	1.1566919948865282
8	0.3625233925526636	0.9266407152340004
9	0.2769898707953416	0.796510954648858
>10	2.5082459344405135	18.31277392399162
>50	0.698270478220862	15.354616942847638
>100	0.10082649954579265	4.853081034335804
>500	0.0050100123997909385	1.0955149114780458
>1k	0.006262515499738673	5.12500884523967
>5k	0.001878754649921602	3.345372384475151
>10k+	0.001878754649921602	8.993427070555663
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTC	16126	3.195482017239671	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC	14748	2.9224214802338255	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCG	14072	2.7884672545328444	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6133	1.215297731100763	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCT	5323	1.054790448825919	No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	5263	1.0429010205092637	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTC	4386	0.8691172099474884	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGGATAAT	4148	0.8219558109580898	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCT	3588	0.7109878133359755	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGGATAATCGTA	3051	0.6045774299019122	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTT	3030	0.6004161299910828	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	2305	0.45675220449816706	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC	1407	0.27880709402556225	No Hit
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	1363	0.2700881799266819	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGGATAATCGT	1231	0.24393143763004063	No Hit
TCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	1104	0.21876548102645396	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCG	949	0.18805112454176162	No Hit
ACTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	828	0.1640741107698405	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTAT	773	0.15317546814623997	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATG	663	0.13137818289903894	No Hit
TTGATGCGGGATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTAT	594	0.11770534033488556	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCGGATAA	568	0.11255325473100168	No Hit
TTGTTGGGGACTCTTAAAATGTAGTTTCAGTTTGTAAAGACCAAATTCCAA	567	0.11235509759239078	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	533	0.10561775487961952	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5027246606559002	0.0
2	0.0	0.0	0.0	2.4288120479540276	0.0
3	0.0	0.0	0.0	3.0468641632814824	0.0
4	0.0	0.0	0.0	5.112454176161696	0.0
5	0.0	0.0	0.0	10.594075101555534	0.0
6	0.0	0.0	0.0	12.72287724165263	0.0
7	0.0	0.0	0.0	14.492024175170911	0.0
8	0.0	0.0	0.0	17.662934707222828	0.0
9	0.0	0.0	0.0	18.50906568909145	0.0
10	0.0	0.0	0.0	23.242247101951847	0.0
11	0.0	0.0	0.0	25.140592489844447	0.0
12	0.0	0.0	0.0	30.58218567323888	0.0
13	0.0	0.0	0.0	31.818488061032397	0.0
14	0.0	0.0	0.0	32.40245714851878	0.0
15	1.9815713861091845E-4	0.0	0.0	33.57198058060042	0.0
16	3.963142772218369E-4	0.0	0.0	34.6642227286238	0.0
17	3.963142772218369E-4	0.0	0.0	35.56504508074904	0.0
18	0.0011889428316655107	0.0	0.0	37.0325968493015	0.0
19	0.0011889428316655107	0.0	0.0	38.596849301496086	0.0
20	0.0011889428316655107	0.0	0.0	39.36748241355395	0.0
21	0.0013870999702764292	0.0	0.0	39.94392152977311	0.0
22	0.0013870999702764292	0.0	0.0	40.55979391657584	0.0
23	0.0013870999702764292	0.0	0.0	41.127315961557514	0.0
24	0.0013870999702764292	0.0	0.0	41.518478153175465	0.0
25	0.0013870999702764292	0.0	0.0	41.83790746061627	0.0
26	0.0013870999702764292	0.0	0.0	42.20093133855147	0.0
27	0.0013870999702764292	0.0	0.0	42.55028237392252	0.0
28	0.0013870999702764292	0.0	0.0	42.845338353314176	0.0
29	0.0013870999702764292	0.0	0.0	43.12275834736946	0.0
30	0.0013870999702764292	0.0	0.0	43.47765778262162	0.0
31	0.0023778856633310213	0.0	0.0	43.79906866144853	0.0
32	0.0023778856633310213	0.0	0.0	44.08837808382047	0.0
33	0.0023778856633310213	0.0	0.0	44.39472902011295	0.0
34	0.0023778856633310213	0.0	0.0	44.69255919944516	0.0
35	0.0023778856633310213	0.0	0.0	44.99217279302487	0.0
36	0.0023778856633310213	0.0	0.0	45.259288615872386	0.0
37	0.00257604280194194	0.0	0.0	45.52580996730407	0.0
38	0.00257604280194194	0.0	0.0	45.76082433369662	0.0
39	0.00257604280194194	0.0	0.0	46.053502427424945	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.0295203E-4	45.000004	25
AGACTGC	20	7.0295203E-4	45.000004	13
CGGCGAA	40	6.8012014E-9	45.000004	31
CTCACGA	20	7.0295203E-4	45.000004	24
AGATCCG	20	7.0295203E-4	45.000004	17
CTGTACG	20	7.0295203E-4	45.000004	1
CGCGGTT	20	7.0295203E-4	45.000004	18
TAAAGCG	20	7.0295203E-4	45.000004	1
AACCCTA	20	7.0295203E-4	45.000004	18
AGCCCTT	20	7.0295203E-4	45.000004	17
TCCGAGT	20	7.0295203E-4	45.000004	15
TGGACCG	20	7.0295203E-4	45.000004	45
CGTGATT	20	7.0295203E-4	45.000004	23
GATCCAT	20	7.0295203E-4	45.000004	11
GCAAACA	40	6.8012014E-9	45.000004	33
TTCGTAC	40	6.8012014E-9	45.000004	33
GTTAGAA	20	7.0295203E-4	45.000004	9
TTACTAG	40	6.8012014E-9	45.000004	1
ATGTCGC	20	7.0295203E-4	45.000004	34
GTAGCAA	20	7.0295203E-4	45.000004	26
>>END_MODULE