##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1178109
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.806172433959844	28.0	25.0	31.0	16.0	33.0
2	27.69070179414638	30.0	25.0	31.0	16.0	33.0
3	27.67584578336979	30.0	26.0	31.0	16.0	33.0
4	30.772465875398627	33.0	28.0	35.0	19.0	37.0
5	31.950001230785947	33.0	32.0	35.0	26.0	37.0
6	31.148914913645513	35.0	30.0	35.0	17.0	37.0
7	31.9140537929852	33.0	31.0	35.0	26.0	35.0
8	33.018468579732435	35.0	32.0	35.0	28.0	37.0
9	34.239325902781495	35.0	33.0	37.0	28.0	39.0
10	33.8213060081877	35.0	32.0	38.0	27.0	39.0
11	34.05098424678871	37.0	32.0	39.0	27.0	39.0
12	33.64711754175548	35.0	32.0	37.0	26.0	39.0
13	33.633995665935835	35.0	32.0	38.0	25.0	39.0
14	34.547593643712084	37.0	33.0	39.0	25.0	40.0
15	35.17805228548462	37.0	33.0	39.0	27.0	41.0
16	35.39507380047177	37.0	34.0	39.0	29.0	41.0
17	34.712055505899706	36.0	32.0	39.0	27.0	40.0
18	34.64633323402164	36.0	33.0	39.0	27.0	40.0
19	34.08288282323622	36.0	32.0	39.0	25.0	40.0
20	33.7534515057605	35.0	32.0	38.0	25.0	40.0
21	34.49416225493567	36.0	33.0	39.0	27.0	40.0
22	34.757136224237314	36.0	33.0	39.0	27.0	40.0
23	35.02516405527842	36.0	33.0	39.0	28.0	40.0
24	34.82093422595023	36.0	33.0	39.0	27.0	40.0
25	34.142224531006896	35.0	33.0	39.0	25.0	40.0
26	33.99332998899083	36.0	32.0	39.0	24.0	40.0
27	34.4959260985189	36.0	33.0	39.0	26.0	40.0
28	34.27796154685178	36.0	33.0	39.0	25.0	40.0
29	34.32175291080876	36.0	33.0	39.0	25.0	40.0
30	33.91956007466202	36.0	33.0	39.0	24.0	40.0
31	34.03524716303839	36.0	33.0	39.0	24.0	40.0
32	33.513600184702774	36.0	32.0	39.0	21.0	40.0
33	33.40455000343771	36.0	32.0	39.0	19.0	40.0
34	33.25035374485722	36.0	33.0	39.0	18.0	40.0
35	33.15366829385057	37.0	32.0	40.0	16.0	40.0
36	33.044913501212534	37.0	32.0	40.0	15.0	40.0
37	32.94072195357136	37.0	32.0	40.0	15.0	40.0
38	33.0367020369083	37.0	32.0	40.0	15.0	40.0
39	32.67597225723596	36.0	31.0	40.0	13.0	40.0
40	32.5707171407739	36.0	31.0	40.0	12.0	40.0
41	32.1889375261542	36.0	30.0	39.0	10.0	40.0
42	32.41318757432462	36.0	31.0	39.0	11.0	40.0
43	32.5911363040262	36.0	31.0	40.0	12.0	40.0
44	32.505168876564056	36.0	31.0	40.0	10.0	40.0
45	32.59287213661894	36.0	31.0	39.0	11.0	40.0
46	32.22871907438106	36.0	31.0	39.0	10.0	40.0
47	32.359104293405785	36.0	31.0	39.0	11.0	40.0
48	32.2140498035411	36.0	31.0	39.0	10.0	40.0
49	32.32941264348205	36.0	31.0	39.0	10.0	40.0
50	32.19811749167522	35.0	31.0	39.0	10.0	40.0
51	30.218735278314654	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	55.0
10	63.0
11	84.0
12	58.0
13	70.0
14	155.0
15	337.0
16	820.0
17	1641.0
18	3190.0
19	5205.0
20	7887.0
21	11265.0
22	14978.0
23	19748.0
24	25346.0
25	31386.0
26	34457.0
27	34884.0
28	36152.0
29	40725.0
30	50468.0
31	64175.0
32	80892.0
33	101112.0
34	115995.0
35	123193.0
36	140588.0
37	139213.0
38	84800.0
39	9148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.763036357416844	17.428523167211182	20.359915763312223	21.44852471205975
2	32.2033020713703	33.89796699626266	21.888551908185068	12.010179024181973
3	23.20209759877906	32.909009268242585	33.6078410401754	10.281052092802959
4	20.359745999733473	21.288013248349685	45.00933275274189	13.34290799917495
5	29.169032746545525	24.258366585774322	32.326974838491175	14.24562582918898
6	16.283382946739223	41.333526863813105	32.36160660855659	10.021483580891072
7	65.50336174326824	2.961101222382649	29.080161513068823	2.455375521280289
8	62.43794080174246	13.844729137966011	18.75887545210163	4.958454608189904
9	59.098097035163974	4.0701666823697975	19.02328222600795	17.808454056458274
10	36.095556523207954	24.82028403144361	25.828425043862662	13.255734401485771
11	31.436395104357917	20.2254630089406	33.90492730299149	14.433214583709997
12	26.244091166437062	17.739614925274317	35.7084106818639	20.30788322642472
13	19.654632975386825	24.962800555805956	42.04212004152417	13.340446427283045
14	17.29491923073332	30.371807702003807	33.560731647071705	18.77254142019117
15	12.785574170131966	20.192189347505195	52.14500525842685	14.877231223935986
16	14.37524032156617	22.813593648804993	38.40179474055457	24.409371289074272
17	14.81671050811088	21.338517913028422	40.117001058475914	23.727770520384787
18	15.520720069195635	23.043368652645892	40.112502323638985	21.323408954519486
19	18.37189937433633	25.079767661566123	35.01238000898049	21.535952955117057
20	20.6995278026057	22.697899769885467	41.71031712685328	14.89225530065554
21	18.32326210902387	29.61865158487033	35.40470363947648	16.65338266662932
22	16.003188160008964	20.423746868922997	38.84852759804059	24.724537373027452
23	15.937574536821295	30.3010162896642	36.64516610941772	17.116243064096786
24	19.958509781352998	20.492586000106954	36.193679871726644	23.35522434681341
25	14.684634443841786	31.63060463845026	33.55063071413596	20.134130203571996
26	15.031546317021599	24.6312522865032	36.04768319399987	24.28951820247532
27	18.62985513225007	27.562899527972366	33.47219994075251	20.33504539902505
28	12.91408519924727	24.46148870775115	42.18854112819781	20.435884964803765
29	18.232693239759648	20.966226384825173	36.070771040710156	24.730309334705023
30	14.956680578791945	24.950832223503937	40.76965713698817	19.322830060715944
31	21.365340558471246	22.003906259947083	30.997556253283864	25.633196928297806
32	21.653938642349733	30.565677708938647	30.32452854532136	17.455855103390263
33	18.06420288784824	21.559719856142344	29.937552467556056	30.438524788453357
34	19.091272539298146	24.43542999841271	33.807398126998436	22.665899335290707
35	15.66434005681987	27.100208894083654	33.01137670623007	24.22407434286641
36	19.213926724946504	22.217553723806542	28.445160846746774	30.12335870450018
37	16.512988187001373	27.802351055802138	37.88588322472709	17.798777532469405
38	18.064881942163247	26.398236495943923	24.958811111705284	30.578070450187546
39	19.72253840688765	27.363512204728085	33.050422329343036	19.863527059041225
40	18.11903652378515	26.008459319129212	31.506507462382512	24.36599669470312
41	15.766792376596733	25.151917182535744	32.22010866566676	26.861181775200766
42	18.88017152911997	26.870264126664	33.07469852110459	21.174865823111443
43	20.793407061655586	23.64526542111129	27.311734313208706	28.249593204024414
44	20.490803482530055	24.211342074460003	32.8027372679438	22.495117175066145
45	17.118789517778065	25.020435290792275	30.006476480529393	27.854298710900267
46	24.72445249123808	27.881376001711217	27.93782239164627	19.456349115404432
47	16.30655567523888	22.97953754703512	39.506955638230416	21.20695113949558
48	21.169433388591376	25.0635552397953	29.15095292540843	24.616058446204892
49	18.20120209590114	21.834736853720667	37.40146285275811	22.562598197620083
50	20.737554844246162	22.572020076240822	32.23368975196692	24.45673532754609
51	19.17878566414483	23.016206480045565	28.643359824939797	29.16164803086981
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	64.0
1	311.5
2	559.0
3	12147.5
4	23736.0
5	19662.0
6	15588.0
7	15730.5
8	15873.0
9	15865.5
10	15858.0
11	15491.5
12	15125.0
13	14530.5
14	13936.0
15	13269.5
16	12603.0
17	11484.5
18	10366.0
19	9794.5
20	9223.0
21	8603.0
22	7983.0
23	8311.5
24	8640.0
25	8995.5
26	10098.0
27	10845.0
28	13443.5
29	16042.0
30	16583.0
31	17124.0
32	19918.0
33	22712.0
34	25528.5
35	28345.0
36	29870.0
37	31395.0
38	34754.0
39	38113.0
40	46176.0
41	54239.0
42	61101.5
43	67964.0
44	74433.5
45	80903.0
46	159788.5
47	238674.0
48	168873.5
49	99073.0
50	91431.0
51	83789.0
52	71984.0
53	60179.0
54	51211.0
55	42243.0
56	38207.5
57	34172.0
58	31321.5
59	28471.0
60	26876.5
61	25282.0
62	21333.0
63	17384.0
64	13621.5
65	9859.0
66	7197.0
67	4535.0
68	3694.0
69	2853.0
70	2520.5
71	2188.0
72	1665.0
73	1142.0
74	930.5
75	626.5
76	534.0
77	384.0
78	234.0
79	176.5
80	119.0
81	81.5
82	44.0
83	26.5
84	9.0
85	8.5
86	8.0
87	6.0
88	4.0
89	4.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1178109.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.449796759022977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.39612104150785	22.892065730802702
2	8.15011412172092	4.4743795240816455
3	2.623239503010891	2.1602217362366822
4	1.1739824117019313	1.289023143995426
5	0.6605497689220262	0.9065978453064607
6	0.40681223175223663	0.670014784841007
7	0.2900804426571199	0.5573854436293753
8	0.21429381130361683	0.47058572536005616
9	0.17091124213340092	0.4222330974354618
>10	1.598050954105923	10.262031339931868
>50	0.6987150105184924	14.05878386280409
>100	0.6088756715826584	24.987486850386908
>500	0.003174534262683292	0.6402199333558569
>1k	0.0019047205576099753	0.8719260450740035
>5k	0.0019047205576099753	3.9525106488573507
>10k+	0.0012698137050733169	11.384534287901085
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	44329	3.7627248412498338	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	39840	3.381690488740855	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	35358	3.001250308757509	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	11119	0.9438006160720273	TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	9847	0.8358309799857229	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9224	0.782949625204459	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTC	8234	0.6989166537221938	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	7523	0.6385657014758397	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTA	5337	0.453014109899848	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACTACT	5193	0.4407911322297003	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCACTACTCGT	2230	0.18928639030853683	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	1823	0.15473950203249445	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTAT	1787	0.15168375761495753	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1557	0.13216094605847167	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	1468	0.12460646680400542	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.395271575041019E-4	0.0	0.0	0.17901569379403773	0.0
2	3.395271575041019E-4	0.0	0.0	1.4350964129804629	0.0
3	3.395271575041019E-4	0.0	0.0	1.5998519661593282	0.0
4	3.395271575041019E-4	0.0	0.0	2.6902434324837516	0.0
5	3.395271575041019E-4	0.0	0.0	6.3887976409653096	0.0
6	3.395271575041019E-4	0.0	0.0	6.834342153400067	0.0
7	3.395271575041019E-4	0.0	0.0	7.792148264719139	0.0
8	3.395271575041019E-4	0.0	0.0	9.128866683812788	0.0
9	3.395271575041019E-4	0.0	0.0	9.45107795628418	0.0
10	3.395271575041019E-4	0.0	0.0	13.618009878542647	0.0
11	3.395271575041019E-4	0.0	0.0	14.15123727940284	0.0
12	3.395271575041019E-4	0.0	0.0	18.890102698476966	0.0
13	3.395271575041019E-4	0.0	0.0	19.554727109291246	0.0
14	3.395271575041019E-4	0.0	0.0	19.856566752312393	0.0
15	4.244089468801274E-4	0.0	0.0	20.550135853303896	0.0
16	4.244089468801274E-4	0.0	0.0	21.06545319660575	0.0
17	4.244089468801274E-4	0.0	0.0	21.40447106337359	0.0
18	5.092907362561529E-4	0.0	0.0	21.900605122276463	0.0
19	5.092907362561529E-4	0.0	0.0	22.636869763324107	0.0
20	5.092907362561529E-4	0.0	0.0	22.931918863195172	0.0
21	6.790543150082038E-4	0.0	0.0	23.13572003948701	0.0
22	6.790543150082038E-4	0.0	0.0	23.40097563128709	0.0
23	6.790543150082038E-4	0.0	0.0	23.600278072741997	0.0
24	6.790543150082038E-4	0.0	0.0	23.761808117924573	0.0
25	6.790543150082038E-4	0.0	0.0	23.882849549574786	0.0
26	6.790543150082038E-4	0.0	0.0	24.021631275204587	0.0
27	6.790543150082038E-4	0.0	0.0	24.188593754907227	0.0
28	6.790543150082038E-4	0.0	0.0	24.318717538020675	0.0
29	6.790543150082038E-4	0.0	0.0	24.460555008068013	0.0
30	6.790543150082038E-4	0.0	0.0	24.635496375972004	0.0
31	8.488178937602548E-4	0.0	0.0	24.79142422305576	0.0
32	8.488178937602548E-4	0.0	0.0	24.931054766579322	0.0
33	8.488178937602548E-4	0.0	0.0	25.073231763784165	0.0
34	8.488178937602548E-4	0.0	0.0	25.252586984735707	0.0
35	8.488178937602548E-4	0.0	0.0	25.404780033086922	0.0
36	8.488178937602548E-4	0.0	0.0	25.555699854597496	0.0
37	8.488178937602548E-4	0.0	0.0	25.712476519575013	0.0
38	8.488178937602548E-4	0.0	0.0	25.86526374045186	0.0
39	8.488178937602548E-4	0.0	0.0	26.021955523640003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	25	3.890865E-5	45.0	16
CTTAACG	20	7.033359E-4	45.0	16
CGTGGTA	20	7.033359E-4	45.0	45
CGGGTGA	75	0.0	45.0	6
GTACCGA	25	3.890865E-5	45.0	14
ACCGGTA	20	7.033359E-4	45.0	42
TACCCGA	20	7.033359E-4	45.0	29
GCGACTA	25	3.890865E-5	45.0	11
TCCGCGA	20	7.033359E-4	45.0	21
GTCGATG	35	1.2119563E-7	45.0	1
CACGGTC	20	7.033359E-4	45.0	4
CGACGTG	20	7.033359E-4	45.0	11
CGTGCGC	25	3.890865E-5	45.0	32
GCGAAGT	20	7.033359E-4	45.0	45
CCGGCAT	25	3.890865E-5	45.0	12
ACACCGA	25	3.890865E-5	45.0	27
CGTGACG	20	7.033359E-4	45.0	24
GACGTAC	20	7.033359E-4	45.0	41
TAGCGCG	45	3.8562575E-10	45.0	1
GGTCCGT	25	3.890865E-5	45.0	29
>>END_MODULE