FastQCFastQC Report
Sat 18 Jun 2016
SRR3548245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561650
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGC119012.1189352799786345No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCG111881.9919878928158103No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTC99921.7790438885426867No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64561.149470310691712No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC44650.7949790794979079No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGCATT33660.5993056173773702No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT30540.5437550075669901No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTC26610.47378260482506895No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCT26000.4629217484198344No Hit
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT24940.4440487848304104No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTA20500.3649959939464079No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC12590.22416095433098904No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGC11640.20724650583103357No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTT10580.18837354224160954No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC9580.17056885961007745No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGT8660.15418855158906794No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCG8080.1438618356627793No Hit
ACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC7940.1413691800943648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6120.10896465770497642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA302.1635115E-645.00000419
GCGAACC302.1635115E-645.00000433
CCATCGA302.1635115E-645.00000441
CTAGTAG302.1635115E-645.0000041
CATCGAA302.1635115E-645.00000442
ACGTAGG302.1635115E-645.0000042
TTCATCG302.1635115E-645.00000415
TCGACAA302.1635115E-645.00000419
ACCTTAC600.045.00000443
AACACGC302.1635115E-645.00000421
CTTCGCG207.0302025E-445.028
AGCGTCC253.8882503E-545.019
CAGAACG406.8048394E-945.012
TCGCAGG207.0302025E-445.02
ACGCCCT207.0302025E-445.031
AGTTTCG207.0302025E-445.01
GTATTCC207.0302025E-445.035
TACCAGA207.0302025E-445.024
CAACGAC207.0302025E-445.012
CCGATCT207.0302025E-445.011
Produced by FastQC (version 0.11.5)