SRR3548243_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453896
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	10673	2.3514197084794755	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	10277	2.264175053316178	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	10143	2.2346528720235472	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7863	1.7323351604772899	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	3101	0.6831961506600631	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT	3072	0.6768070218728519	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTCTGAAT	2908	0.640675397007244	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTC	2743	0.60432345735587	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT	2637	0.5809700900646845	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTA	1945	0.42851225831468004	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1756	0.38687276380492447	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	1566	0.34501295450940306	No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT	1411	0.3108641627156882	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTT	1282	0.28244355535188675	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	1002	0.22075541533743415	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGT	800	0.17625182861272184	No Hit
TCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	772	0.1700830146112766	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	705	0.15532192396496114	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	591	0.13020603838764827	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCC	552	0.12161376174277808	No Hit
ACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	520	0.11456368859826922	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTAT	465	0.10244637538114458	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTATG	30	2.1625874E-6	45.000004	1
TCGTTGA	35	1.2097553E-7	45.000004	24
GTACCAC	20	7.0287695E-4	45.000004	33
TCGCAGA	30	2.1625874E-6	45.000004	26
AGATCCA	30	2.1625874E-6	45.000004	17
CGAACCC	20	7.0287695E-4	45.000004	34
ATTAGTG	20	7.0287695E-4	45.000004	1
CTACTTG	20	7.0287695E-4	45.000004	22
GGTAATC	20	7.0287695E-4	45.000004	8
GTACATC	20	7.0287695E-4	45.000004	36
TACTTAA	30	2.1625874E-6	45.000004	18
ATCTAAC	20	7.0287695E-4	45.000004	21
GTCGAAA	40	6.7993824E-9	45.000004	27
TACCAGG	20	7.0287695E-4	45.000004	2
AAATAGG	20	7.0287695E-4	45.000004	2
CGCATGT	20	7.0287695E-4	45.000004	13
GAGTAGT	20	7.0287695E-4	45.000004	31
CGTTGAT	35	1.2097553E-7	45.000004	25
CCTACTC	20	7.0287695E-4	45.000004	10
CGCGCCT	30	2.1625874E-6	45.000004	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content