##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453896
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.62867925692229	30.0	25.0	31.0	16.0	33.0
2	27.93167377549042	31.0	26.0	33.0	16.0	33.0
3	27.906709907115285	31.0	25.0	33.0	16.0	34.0
4	31.965699191004106	35.0	32.0	35.0	19.0	37.0
5	33.073730546204416	35.0	32.0	35.0	28.0	37.0
6	32.001641345153956	35.0	32.0	35.0	25.0	37.0
7	33.64722755873592	35.0	33.0	35.0	30.0	37.0
8	33.194090716816184	35.0	33.0	37.0	28.0	37.0
9	35.87216675185505	37.0	35.0	39.0	31.0	39.0
10	35.2564728484058	37.0	34.0	39.0	30.0	39.0
11	35.54167694802334	37.0	35.0	39.0	30.0	39.0
12	35.46419444101733	37.0	34.0	39.0	30.0	39.0
13	35.21465269577172	37.0	34.0	39.0	30.0	39.0
14	35.89011800059926	38.0	34.0	40.0	27.0	41.0
15	36.35479933729312	38.0	34.0	40.0	30.0	41.0
16	36.18619683804219	37.0	34.0	40.0	31.0	41.0
17	35.61101441739958	37.0	34.0	39.0	30.0	41.0
18	35.68810256093907	37.0	34.0	39.0	30.0	40.0
19	35.173229109752015	37.0	34.0	39.0	28.0	40.0
20	34.93023511993937	36.0	34.0	39.0	27.0	40.0
21	35.47956360047235	37.0	34.0	39.0	30.0	40.0
22	35.910770308616954	37.0	34.0	40.0	30.0	41.0
23	36.036766131448616	37.0	34.0	40.0	31.0	41.0
24	35.990618996422086	37.0	35.0	40.0	30.0	41.0
25	35.39162054743818	37.0	34.0	40.0	29.0	41.0
26	35.5078828630347	37.0	34.0	40.0	29.0	41.0
27	35.59401713167774	37.0	34.0	40.0	30.0	41.0
28	35.403220120908756	37.0	34.0	40.0	29.0	41.0
29	35.47833644711564	37.0	34.0	40.0	29.0	41.0
30	34.90896813366939	37.0	34.0	40.0	26.0	41.0
31	34.98638234309181	37.0	34.0	40.0	27.0	41.0
32	34.5709788145302	37.0	34.0	40.0	25.0	41.0
33	34.44262342034298	37.0	33.0	40.0	24.0	41.0
34	34.184835292666165	37.0	34.0	40.0	22.0	41.0
35	34.07245492359483	37.0	33.0	40.0	21.0	41.0
36	33.7181711229004	37.0	33.0	40.0	18.0	41.0
37	33.846057246593936	37.0	33.0	40.0	18.0	41.0
38	33.52030200750833	37.0	33.0	40.0	18.0	41.0
39	33.53358919223787	37.0	33.0	40.0	18.0	41.0
40	33.29601274300721	36.0	33.0	40.0	18.0	41.0
41	32.90055651514884	36.0	32.0	40.0	15.0	41.0
42	33.137577771119375	36.0	33.0	40.0	15.0	41.0
43	33.200277156000496	36.0	33.0	40.0	15.0	41.0
44	33.34643398487759	36.0	33.0	40.0	17.0	41.0
45	33.41690607540053	36.0	33.0	40.0	18.0	41.0
46	33.143466785342895	36.0	33.0	40.0	17.0	40.0
47	32.977464000564005	35.0	32.0	39.0	18.0	41.0
48	33.08709263796112	36.0	33.0	39.0	18.0	40.0
49	33.156159120150875	36.0	33.0	40.0	18.0	40.0
50	33.00725276274741	35.0	32.0	40.0	17.0	40.0
51	30.991760227012357	34.0	29.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	12.0
10	17.0
11	20.0
12	15.0
13	17.0
14	35.0
15	49.0
16	125.0
17	301.0
18	682.0
19	1482.0
20	2568.0
21	3425.0
22	4506.0
23	5130.0
24	6382.0
25	8108.0
26	9989.0
27	10436.0
28	10236.0
29	11179.0
30	13781.0
31	18217.0
32	25884.0
33	38631.0
34	48875.0
35	49526.0
36	59347.0
37	67962.0
38	48374.0
39	8584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.16690166910482	20.02397024869133	23.143847929922273	17.66528015228158
2	31.496642402664925	34.10627104006204	22.631836367802315	11.765250189470716
3	23.06695806968997	32.997426723302254	31.852230466891097	12.08338474011668
4	21.64130109099882	21.61155859492042	43.63224174700812	13.114898567072633
5	29.621543261011336	24.744875478083085	30.44463930063274	15.188941960272837
6	17.487045490596962	40.70051289282126	30.084204311119727	11.728237305462045
7	64.00452967199534	5.485838165570968	26.743350899765584	3.7662812626681004
8	60.358099652783906	13.840615471385515	17.656247246065178	8.145037629765408
9	56.309154519978144	5.974936989971271	19.488825634065954	18.22708285598463
10	41.13783774256658	19.162980065918184	24.589994183689655	15.10918800782558
11	32.91568993778311	20.648562666337664	29.542890882492905	16.892856513386327
12	26.55960836843682	15.49914517863123	35.103195445652744	22.8380510072792
13	22.3482912385216	25.20268960290463	38.930503903978	13.518515254595767
14	14.572501189699844	34.73549006820946	31.15449354037048	19.53751520172022
15	10.962863739711299	21.349383999858997	49.39259213564341	18.295160124786296
16	11.75379382061089	22.430909283183816	38.469164742584205	27.346132153621095
17	12.259856883515166	24.928177379840317	35.65993090928318	27.152034827361334
18	15.223311066852318	23.25246311930486	36.29135308528826	25.232872728554558
19	17.72168073743765	26.85945679186422	31.314662389622296	24.10420008107584
20	20.437721416359693	24.6245836050549	36.782214427974694	18.15548055061071
21	17.356398822637782	31.655709677987907	33.68436822532034	17.30352327405397
22	14.696538414086046	22.52696652977775	34.778671766219574	27.997823289916635
23	15.922810511659058	30.796482013500892	31.052267479775104	22.22843999506495
24	18.796816891975254	24.256657912825847	33.19593034527733	23.75059484992157
25	14.415857377020286	31.758376368154817	30.872049985018595	22.9537162698063
26	16.562824964309005	25.710515184095033	34.31557008653965	23.41108976505631
27	21.80367308810829	27.325642878544866	29.29063045279095	21.580053580555898
28	14.73619507552391	26.394592593898164	38.635502405837464	20.23370992474047
29	22.67832278759892	19.860496677653032	33.59139538572713	23.869785149020924
30	21.892239631986183	27.06523080176957	33.00139238944604	18.04113717679821
31	24.04846044027707	23.85854909494686	28.044750339284768	24.0482401254913
32	25.789608192184993	27.699737384775368	25.716463683310714	20.794190739728926
33	22.915822126654565	23.61223716446058	28.243914905617146	25.228025803267705
34	26.398337945256184	23.507147011650247	28.839866401113913	21.25464864197966
35	22.856998078855067	25.805470856760138	26.473024657630823	24.864506406753968
36	26.468838676701274	24.59902708990606	30.4287766360576	18.50335759733507
37	23.694634894337028	23.819994007437828	31.906868533761042	20.578502564464106
38	19.706937271974198	26.276503868727634	30.887031390450677	23.12952746884749
39	25.048248938082736	24.12028306043675	27.513791705588947	23.31767629589157
40	22.68052593545658	24.55099846660909	32.21024199376068	20.558233604173644
41	21.230854645116942	27.566006309815467	26.644870190528223	24.558268854539367
42	23.096920880554137	26.579436699155757	29.95664205016127	20.367000370128842
43	24.252251617110527	24.864065777182436	28.000907696917356	22.88277490878968
44	22.807206937271975	23.12996809841902	30.557881100516415	23.504943863792587
45	20.455126284435202	22.96649452738072	28.607874931702415	27.97050425648166
46	27.13991751414421	25.494827008830217	27.588478417963586	19.77677705906199
47	18.36742337451751	23.20906110650898	36.91220015157658	21.511315367396936
48	22.0504256481661	23.277799319667942	29.331168373371874	25.340606658794083
49	19.972196274036342	19.31543789766819	36.02961911979837	24.6827467084971
50	20.267638401748417	21.23856566261875	33.407652854398364	25.086143081234468
51	19.573646826585826	21.817112290040008	27.412887533708165	31.196353349666
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	657.0
1	648.0
2	639.0
3	5925.5
4	11212.0
5	7357.5
6	3503.0
7	3580.0
8	3657.0
9	4119.5
10	4582.0
11	4858.5
12	5135.0
13	4932.5
14	4730.0
15	4457.5
16	4185.0
17	3990.0
18	3795.0
19	3502.0
20	3209.0
21	3108.0
22	3007.0
23	2870.5
24	2734.0
25	2674.5
26	2923.0
27	3231.0
28	3394.5
29	3558.0
30	3858.0
31	4158.0
32	4409.0
33	4660.0
34	5044.0
35	5428.0
36	5929.5
37	6431.0
38	7407.0
39	8383.0
40	11121.5
41	13860.0
42	18181.0
43	22502.0
44	27546.5
45	32591.0
46	58309.0
47	84027.0
48	70432.5
49	56838.0
50	51292.0
51	45746.0
52	37400.0
53	29054.0
54	24686.0
55	20318.0
56	18069.0
57	15820.0
58	14652.5
59	13485.0
60	11658.5
61	9832.0
62	8476.5
63	7121.0
64	6045.0
65	4969.0
66	3853.0
67	2737.0
68	2339.5
69	1942.0
70	1632.5
71	1323.0
72	1163.0
73	1003.0
74	804.5
75	466.5
76	327.0
77	249.0
78	171.0
79	117.5
80	64.0
81	51.5
82	39.0
83	22.0
84	5.0
85	3.0
86	1.0
87	1.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	453896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.688746324728704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.60438785785348	24.908745068381037
2	9.194488100006069	5.827236019736583
3	3.5332520660647604	3.358929852685493
4	1.7758835067684424	2.251020877930192
5	1.1253767065876177	1.783088848740683
6	0.7600892276787399	1.445176483204233
7	0.5536391057503592	1.228089042430095
8	0.37403303082885686	0.9482110264804061
9	0.29272587333101585	0.8348504348404105
>10	2.903842292876946	22.12913199571009
>50	0.7849510971082458	16.778379089898337
>100	0.08262643127455219	3.7914721088288426
>500	0.004201343963112824	0.8742552881597494
>1k	0.00770246393237351	5.197381120448175
>5k	7.002239938521372E-4	1.7447384088325149
>10k+	0.002100671981556412	6.899294333693169
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	10673	2.3514197084794755	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	10277	2.264175053316178	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	10143	2.2346528720235472	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7863	1.7323351604772899	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	3101	0.6831961506600631	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT	3072	0.6768070218728519	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTCTGAAT	2908	0.640675397007244	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTC	2743	0.60432345735587	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT	2637	0.5809700900646845	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTA	1945	0.42851225831468004	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1756	0.38687276380492447	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	1566	0.34501295450940306	No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT	1411	0.3108641627156882	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTT	1282	0.28244355535188675	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	1002	0.22075541533743415	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGT	800	0.17625182861272184	No Hit
TCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	772	0.1700830146112766	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	705	0.15532192396496114	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	591	0.13020603838764827	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCC	552	0.12161376174277808	No Hit
ACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	520	0.11456368859826922	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTAT	465	0.10244637538114458	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33642067786453284	0.0
2	0.0	0.0	0.0	1.8138516312106738	0.0
3	0.0	0.0	0.0	2.3201350089007176	0.0
4	0.0	0.0	0.0	3.7803814089571177	0.0
5	0.0	0.0	0.0	7.907317975924	0.0
6	0.0	0.0	0.0	9.474196732291098	0.0
7	0.0	0.0	0.0	11.049227135731533	0.0
8	0.0	0.0	0.0	13.836649805241729	0.0
9	0.0	0.0	0.0	14.714824541304615	0.0
10	0.0	0.0	0.0	19.027927102243687	0.0
11	0.0	0.0	0.0	21.115630012161375	0.0
12	2.2031478576590231E-4	0.0	0.0	25.492623860972557	0.0
13	2.2031478576590231E-4	0.0	0.0	26.487785748277137	0.0
14	2.2031478576590231E-4	0.0	0.0	26.90484163773199	0.0
15	2.2031478576590231E-4	0.0	0.0	27.78830392865326	0.0
16	2.2031478576590231E-4	0.0	0.0	28.75989213388089	0.0
17	2.2031478576590231E-4	0.0	0.0	29.5829881735023	0.0
18	2.2031478576590231E-4	0.0	0.0	30.89430177838095	0.0
19	2.2031478576590231E-4	0.0	0.0	32.20231066147311	0.0
20	2.2031478576590231E-4	0.0	0.0	32.84342668805189	0.0
21	2.2031478576590231E-4	0.0	0.0	33.32833953152264	0.0
22	2.2031478576590231E-4	0.0	0.0	33.87207642279289	0.0
23	2.2031478576590231E-4	0.0	0.0	34.31645134568271	0.0
24	2.2031478576590231E-4	0.0	0.0	34.64934698697499	0.0
25	2.2031478576590231E-4	0.0	0.0	34.92474046918237	0.0
26	2.2031478576590231E-4	0.0	0.0	35.21026843153498	0.0
27	2.2031478576590231E-4	0.0	0.0	35.52465763082292	0.0
28	2.2031478576590231E-4	0.0	0.0	35.794763558171915	0.0
29	2.2031478576590231E-4	0.0	0.0	36.06244602287749	0.0
30	2.2031478576590231E-4	0.0	0.0	36.35722720623227	0.0
31	4.4062957153180463E-4	0.0	0.0	36.623808097009004	0.0
32	4.4062957153180463E-4	0.0	0.0	36.86218869520771	0.0
33	4.4062957153180463E-4	0.0	0.0	37.10277244126408	0.0
34	4.4062957153180463E-4	0.0	0.0	37.356354759680634	0.0
35	4.4062957153180463E-4	0.0	0.0	37.6136824294552	0.0
36	4.4062957153180463E-4	0.0	0.0	37.8353191039357	0.0
37	4.4062957153180463E-4	0.0	0.0	38.06907309163333	0.0
38	4.4062957153180463E-4	0.0	0.0	38.291591025256885	0.0
39	4.4062957153180463E-4	0.0	0.0	38.51344801452315	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTATG	30	2.1625874E-6	45.000004	1
TCGTTGA	35	1.2097553E-7	45.000004	24
GTACCAC	20	7.0287695E-4	45.000004	33
TCGCAGA	30	2.1625874E-6	45.000004	26
AGATCCA	30	2.1625874E-6	45.000004	17
CGAACCC	20	7.0287695E-4	45.000004	34
ATTAGTG	20	7.0287695E-4	45.000004	1
CTACTTG	20	7.0287695E-4	45.000004	22
GGTAATC	20	7.0287695E-4	45.000004	8
GTACATC	20	7.0287695E-4	45.000004	36
TACTTAA	30	2.1625874E-6	45.000004	18
ATCTAAC	20	7.0287695E-4	45.000004	21
GTCGAAA	40	6.7993824E-9	45.000004	27
TACCAGG	20	7.0287695E-4	45.000004	2
AAATAGG	20	7.0287695E-4	45.000004	2
CGCATGT	20	7.0287695E-4	45.000004	13
GAGTAGT	20	7.0287695E-4	45.000004	31
CGTTGAT	35	1.2097553E-7	45.000004	25
CCTACTC	20	7.0287695E-4	45.000004	10
CGCGCCT	30	2.1625874E-6	45.000004	17
>>END_MODULE