##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637924
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.601519930273827	28.0	25.0	31.0	16.0	33.0
2	27.33491920667666	30.0	25.0	31.0	16.0	33.0
3	27.097074259629675	30.0	25.0	31.0	16.0	33.0
4	29.47160947072065	32.0	28.0	35.0	19.0	37.0
5	31.624011637749952	33.0	30.0	35.0	26.0	37.0
6	30.81839842990701	33.0	30.0	35.0	17.0	36.0
7	32.06201052162954	35.0	31.0	35.0	26.0	35.0
8	33.0335823703137	35.0	32.0	35.0	28.0	37.0
9	34.21103454329983	37.0	32.0	39.0	27.0	39.0
10	34.146959512418405	37.0	32.0	39.0	27.0	39.0
11	34.48600617001398	37.0	33.0	39.0	27.0	39.0
12	33.89480878599959	37.0	32.0	39.0	25.0	39.0
13	34.103996714342145	37.0	32.0	39.0	25.0	39.0
14	34.656993623064814	37.0	33.0	39.0	25.0	41.0
15	35.771333262269486	38.0	34.0	40.0	29.0	41.0
16	35.56089753638364	37.0	34.0	40.0	27.0	41.0
17	35.121244850483755	37.0	33.0	39.0	27.0	41.0
18	34.832285664123	37.0	33.0	39.0	27.0	40.0
19	34.26224127011995	36.0	32.0	38.0	27.0	40.0
20	34.2271477480076	35.0	32.0	38.0	27.0	40.0
21	34.074087195339885	35.0	33.0	38.0	27.0	40.0
22	34.32738696145622	35.0	33.0	39.0	27.0	40.0
23	34.77393702071093	35.0	33.0	39.0	28.0	40.0
24	34.27214997397809	35.0	33.0	39.0	25.0	40.0
25	33.392462111474096	35.0	32.0	38.0	23.0	40.0
26	33.295394122183836	35.0	31.0	38.0	22.0	40.0
27	33.869677265630386	35.0	33.0	39.0	24.0	40.0
28	33.743720255077406	35.0	33.0	39.0	23.0	40.0
29	33.74452285852233	35.0	33.0	39.0	23.0	40.0
30	32.96019588540328	35.0	31.0	39.0	21.0	40.0
31	32.69308883189847	35.0	31.0	39.0	18.0	40.0
32	32.03237532997661	35.0	31.0	39.0	15.0	40.0
33	31.67704146575454	35.0	30.0	39.0	12.0	40.0
34	31.078473297759608	35.0	29.0	39.0	9.0	40.0
35	30.85802854258501	35.0	27.0	39.0	8.0	40.0
36	30.33531580564456	35.0	25.0	39.0	7.0	40.0
37	30.29927389469592	35.0	24.0	39.0	7.0	40.0
38	30.13644728839172	35.0	24.0	39.0	7.0	40.0
39	29.911666593512706	35.0	23.0	39.0	7.0	40.0
40	29.837153015092706	35.0	23.0	39.0	7.0	40.0
41	29.659882681949576	35.0	23.0	39.0	7.0	40.0
42	29.677626174904848	35.0	23.0	39.0	7.0	40.0
43	29.606559715577404	35.0	23.0	39.0	7.0	40.0
44	29.396122422106707	35.0	23.0	38.0	7.0	40.0
45	29.45927571309435	35.0	23.0	39.0	7.0	40.0
46	29.191308055505043	34.0	22.0	38.0	7.0	40.0
47	29.36258551175375	34.0	23.0	38.0	7.0	40.0
48	29.23804246273851	34.0	23.0	38.0	7.0	40.0
49	29.29485173782457	35.0	23.0	38.0	7.0	40.0
50	29.2018516312288	34.0	22.0	38.0	7.0	40.0
51	27.315410613176493	32.0	19.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	30.0
10	34.0
11	45.0
12	22.0
13	54.0
14	138.0
15	339.0
16	816.0
17	1874.0
18	3525.0
19	5737.0
20	8493.0
21	11276.0
22	14402.0
23	17974.0
24	21693.0
25	25222.0
26	25829.0
27	24016.0
28	22545.0
29	24118.0
30	26760.0
31	32634.0
32	40247.0
33	49160.0
34	55423.0
35	58238.0
36	65732.0
37	64285.0
38	34277.0
39	2969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.74867852596861	21.85871671233564	23.234429179651496	29.158175582044255
2	41.021187476878126	22.551118942068335	24.747618838607735	11.68007474244581
3	23.869144286780244	21.497545162119625	43.11548084097792	11.517829710122209
4	21.608686928223424	25.974724261824296	39.77182234874373	12.644766461208546
5	17.90511095365592	28.681002752679003	40.68776217856673	12.72612411509835
6	15.990462813752107	34.59644095534891	39.53166834920774	9.881427881691236
7	63.471824229845566	7.686495570005205	25.953718624789158	2.887961575360074
8	60.163906672268176	8.300675315554832	25.429204732852188	6.10621327932481
9	55.44923846727824	8.367924705764324	28.680062201766983	7.502774625190462
10	31.27378810014986	24.548692320715322	32.21042005003731	11.96709952909751
11	25.39581517547545	24.014302644202132	37.44944538847888	13.140436791843543
12	22.984085878568607	17.920786802189603	43.22082254312426	15.874304776117532
13	19.48178779917357	25.366187821746788	42.79679084028818	12.355233538791454
14	12.28375166947787	31.826205002476783	39.613182761582884	16.27686056646246
15	10.477737159912467	21.308337670318096	54.777998633066005	13.435926536703432
16	12.02854885534954	21.981772123325037	48.00665910045711	17.98301992086832
17	11.743248412036543	21.55131332259015	41.411045829910776	25.29439243546253
18	15.325806835924027	21.84335438077263	44.31922924987929	18.511609533424046
19	16.823477404831923	26.402361409823115	41.18876229770317	15.585398887641787
20	19.106351226791908	23.27189445764699	40.44729466206006	17.174459653501046
21	14.911180642208164	27.54748214520852	42.1048588860115	15.436478326571818
22	14.114534019726488	22.95351797392793	39.084749907512496	23.84719809883309
23	13.412569522388246	27.340247427593255	39.1708103159624	20.076372734056093
24	15.858628927583851	22.32914892683141	42.44486804070704	19.367354104877695
25	13.32290366877559	24.5026053260263	41.914867601783286	20.259623403414828
26	13.152507195214477	25.86091760146977	41.95123557038142	19.03533963293433
27	16.69979495990118	27.291652297138846	39.550165850477484	16.45838689248249
28	13.24138925640045	25.755262382352758	44.47802559552548	16.52532276572131
29	15.456543412694929	23.108552115926035	39.6171017237163	21.817802747662732
30	18.05371799775522	25.02069212006446	37.01208921438918	19.913500667791148
31	19.699211818335726	27.858804497087426	32.87444899392404	19.567534690652806
32	19.8136455126316	25.582671289996927	33.20426884707269	21.39941435029878
33	20.573297132573785	27.014503295063363	31.497325700240154	20.914873872122698
34	16.811250242975653	26.476508173387426	33.563245778493986	23.14899580514293
35	20.087502586515008	22.49766429856848	32.92226033195177	24.492572782964743
36	21.772029269944383	24.199277656899568	34.65271725158483	19.375975821571224
37	21.924241759206424	24.625817495501032	33.87237351157818	19.577567233714362
38	19.94328478000514	28.646202368934233	32.062440039879355	19.348072811181268
39	20.831791874894186	22.573221888500825	34.561797330089476	22.033188906515512
40	20.859381368313468	23.267661978542897	35.459082900157384	20.41387375298625
41	16.40383493958528	21.74663439531982	34.47605043861024	27.37348022648466
42	21.38467905267712	22.711953148023902	34.32038926267079	21.58297853662819
43	22.672920285175035	21.71308807945774	35.22441544760818	20.389576187759044
44	19.425354744452317	27.467065042230736	30.81088029295026	22.296699920366688
45	19.939209059386386	24.952502178942947	31.964779503514524	23.143509258156143
46	22.24512637869088	25.889604404286402	32.63398147741737	19.231287739605346
47	17.526852728538195	22.821840846245006	36.68571804791793	22.96558837729886
48	18.160940801725598	21.970799029351458	35.69390711119193	24.174353057731015
49	19.280353145515765	19.296185752534782	37.5764197616017	23.84704134034775
50	17.74584433255372	20.756704560417855	37.97113762767979	23.526313479348637
51	16.295985101673555	22.009361616744314	32.166841191113676	29.52781209046846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	353.0
2	590.0
3	10077.5
4	19565.0
5	16383.5
6	13202.0
7	13299.0
8	13396.0
9	13884.0
10	14372.0
11	14311.0
12	14250.0
13	13945.5
14	13641.0
15	13057.0
16	12473.0
17	11991.5
18	11510.0
19	10832.0
20	10154.0
21	9620.5
22	9087.0
23	8725.0
24	8363.0
25	7792.5
26	7037.0
27	6852.0
28	6482.0
29	6112.0
30	6835.0
31	7558.0
32	7993.0
33	8428.0
34	9047.0
35	9666.0
36	10016.5
37	10367.0
38	11924.5
39	13482.0
40	18704.5
41	23927.0
42	30325.0
43	36723.0
44	43798.5
45	50874.0
46	60837.5
47	70801.0
48	70806.5
49	70812.0
50	64012.5
51	57213.0
52	46978.5
53	36744.0
54	30228.5
55	23713.0
56	20350.5
57	16988.0
58	14960.5
59	12933.0
60	11150.0
61	9367.0
62	7690.0
63	6013.0
64	5324.0
65	4635.0
66	3518.0
67	2401.0
68	2093.5
69	1786.0
70	1395.0
71	1004.0
72	871.5
73	739.0
74	561.0
75	328.0
76	273.0
77	183.5
78	94.0
79	73.5
80	53.0
81	38.5
82	24.0
83	18.0
84	12.0
85	7.5
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	637924.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.79444111153959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.69209913522434	36.214542659671764
2	7.826638197359016	6.855264912711316
3	2.9134603937252592	3.827801089314114
4	1.4381760860243178	2.5193647162966593
5	0.8773046272471297	1.9210532917427807
6	0.5786541293428987	1.5205100514874073
7	0.40050965136256883	1.2278067438840892
8	0.3084957963440176	1.080832078891647
9	0.24045988460173606	0.9477725630290501
>10	2.239751563814297	22.417507674055287
>50	0.4125855084300095	12.073925978188305
>100	0.06602827310112588	4.657028669635195
>500	0.0025535796226954766	0.7422488919082372
>1k	0.0029183767116519733	2.358549373921934
>5k	0.0	0.0
>10k+	3.6479708895649667E-4	1.6357913052622557
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10239	1.6050501313636107	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC	2434	0.38155015330979863	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG	2268	0.35552824474388794	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	2231	0.349728180786426	No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	2069	0.3243333061618625	TruSeq Adapter, Index 13 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC	1903	0.2983113975959519	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1423	0.22306732463428244	No Hit
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	1409	0.22087270583956709	No Hit
TCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	1026	0.16083420595556838	No Hit
ACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	842	0.1319906446535951	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCC	730	0.11443369429587223	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	728	0.11412017732519862	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.37042030085088506	0.0
2	0.0	0.0	0.0	1.3934261761589155	0.0
3	0.0	0.0	0.0	1.7989603777252463	0.0
4	0.0	0.0	0.0	2.579147359246556	0.0
5	0.0	0.0	0.0	4.537217599588666	0.0
6	0.0	0.0	0.0	5.806177538390153	0.0
7	0.0	0.0	0.0	6.859751318338862	0.0
8	0.0	0.0	0.0	9.881114364720561	0.0
9	0.0	0.0	0.0	11.02074855311918	0.0
10	0.0	0.0	0.0	13.429812955775295	0.0
11	0.0	0.0	0.0	15.552479605721057	0.0
12	0.0	0.0	0.0	17.90464067819991	0.0
13	0.0	0.0	0.0	18.699249440372206	0.0
14	0.0	0.0	0.0	18.946614330233697	0.0
15	0.0	0.0	0.0	19.40811131106527	0.0
16	0.0	0.0	0.0	20.45463095917382	0.0
17	0.0	0.0	0.0	21.524037346141547	0.0
18	0.0012540678826944902	0.0	0.0	24.002859274772543	0.0
19	0.0012540678826944902	0.0	0.0	24.622368808823623	0.0
20	0.0012540678826944902	0.0	0.0	25.538465397131947	0.0
21	0.0012540678826944902	0.0	0.0	26.159228999065718	0.0
22	0.0012540678826944902	0.0	0.0	26.66555890670362	0.0
23	0.0012540678826944902	0.0	0.0	27.20433782080624	0.0
24	0.0012540678826944902	0.0	0.0	27.602974649017753	0.0
25	0.0012540678826944902	0.0	0.0	27.926367404267594	0.0
26	0.0012540678826944902	0.0	0.0	28.226559903687587	0.0
27	0.0014108263680313016	0.0	0.0	28.54901210802541	0.0
28	0.0014108263680313016	0.0	0.0	28.829453038292964	0.0
29	0.0014108263680313016	0.0	0.0	29.148299797468038	0.0
30	0.0014108263680313016	0.0	0.0	29.553363723578357	0.0
31	0.0018811018240417353	0.0	0.0	29.91453527379437	0.0
32	0.0018811018240417353	0.0	0.0	30.236203685705508	0.0
33	0.0018811018240417353	0.0	0.0	30.55959644095535	0.0
34	0.0018811018240417353	0.0	0.0	30.87562154739436	0.0
35	0.0018811018240417353	0.0	0.0	31.239771508831772	0.0
36	0.0018811018240417353	0.0	0.0	31.542628902502493	0.0
37	0.0018811018240417353	0.0	0.0	31.871978480195132	0.0
38	0.0018811018240417353	0.0	0.0	32.18283055661803	0.0
39	0.0018811018240417353	0.0	0.0	32.48678525968611	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	25	3.8888462E-5	45.000004	1
CGTATGG	35	1.2107921E-7	45.000004	2
ATTAGCG	25	3.8888462E-5	45.000004	1
GTACACT	50	2.1827873E-11	45.000004	18
GACACCC	35	1.2107921E-7	45.000004	9
CCGGATA	35	1.2107921E-7	45.000004	44
GTCTCGA	35	1.2107921E-7	45.000004	21
ACATTAC	25	3.8888462E-5	45.000004	15
CACCTAA	25	3.8888462E-5	45.000004	41
ACGGCAT	25	3.8888462E-5	45.000004	40
TATCTAG	50	2.1827873E-11	45.000004	1
CTTGCGG	35	1.2107921E-7	45.000004	2
TCTCGAC	35	1.2107921E-7	45.000004	22
CCATACC	25	3.8888462E-5	45.000004	32
GGAACGA	25	3.8888462E-5	45.000004	8
GTCCGAC	25	3.8888462E-5	45.000004	26
AGTTATG	35	1.2107921E-7	45.000004	1
TATAGTG	35	1.2107921E-7	45.000004	18
CCGAACT	25	3.8888462E-5	45.000004	12
GGACCGT	25	3.8888462E-5	45.000004	8
>>END_MODULE