SRR3548241_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548241_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	783075
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10454	1.3349934552884462	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	6151	0.7854930881460908	No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	5216	0.66609200906682	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	3163	0.4039204418478434	No Hit
TCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	3061	0.39089486958465025	No Hit
ACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	2388	0.3049516329853462	TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1725	0.22028541327459056	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC	1723	0.2200300098968809	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC	1694	0.21632666092009067	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG	1644	0.20994157647734893	No Hit
AAAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC	1523	0.19448967212591386	No Hit
AGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTG	1411	0.18018708297417232	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCC	1400	0.17878236439676914	No Hit
ACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTG	1101	0.14059955942917346	TruSeq Adapter, Index 13 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTG	884	0.11288829294767425	TruSeq Adapter, Index 13 (95% over 21bp)
GAAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC	826	0.10548159499409379	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT	822	0.10497078823867446	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	810	0.10343836797241644	No Hit
GGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTG	784	0.10011812406219073	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGG	45	3.8380676E-10	45.000004	2
AATTACG	45	3.8380676E-10	45.000004	1
GGCATAG	45	3.8380676E-10	45.000004	1
CGGTCTA	45	3.8380676E-10	45.000004	31
TAAGAAG	45	3.8380676E-10	45.000004	1
TACGTAG	45	3.8380676E-10	45.000004	1
ATCCTAG	20	7.0319074E-4	45.0	14
TATCAAG	20	7.0319074E-4	45.0	1
TAATAGT	25	3.8896633E-5	45.0	31
ATCTCGC	20	7.0319074E-4	45.0	32
CGAACTC	35	1.211265E-7	45.0	13
CATATGG	25	3.8896633E-5	45.0	2
CCCTCGT	200	0.0	45.0	14
CATATCC	20	7.0319074E-4	45.0	29
ATGATCC	75	0.0	45.0	3
GTCGACT	20	7.0319074E-4	45.0	35
GTACACT	25	3.8896633E-5	45.0	18
GTTTCGG	25	3.8896633E-5	45.0	2
CGAAACG	20	7.0319074E-4	45.0	18
TGCCAAA	20	7.0319074E-4	45.0	14

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content