##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	822751
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.101265145833917	28.0	25.0	31.0	16.0	33.0
2	27.60027031264623	30.0	25.0	33.0	16.0	33.0
3	27.556238764826784	31.0	25.0	33.0	16.0	34.0
4	31.516573057948275	33.0	28.0	35.0	19.0	37.0
5	32.9464686156565	35.0	32.0	35.0	28.0	37.0
6	31.613589044559046	35.0	31.0	35.0	19.0	37.0
7	33.5999859009591	35.0	33.0	35.0	30.0	37.0
8	33.477792187429735	35.0	33.0	37.0	28.0	37.0
9	35.984307524390736	38.0	35.0	39.0	31.0	39.0
10	35.378295498881194	37.0	34.0	39.0	31.0	39.0
11	35.8687233440008	37.0	35.0	39.0	31.0	39.0
12	35.94152665873393	37.0	35.0	39.0	31.0	39.0
13	36.02060404666783	37.0	35.0	39.0	32.0	39.0
14	36.60523414739089	38.0	36.0	40.0	31.0	41.0
15	37.07768814623136	38.0	36.0	40.0	32.0	41.0
16	36.50547735584642	38.0	34.0	40.0	32.0	41.0
17	36.28753900025646	38.0	35.0	40.0	31.0	41.0
18	36.25037465770324	38.0	35.0	39.0	31.0	40.0
19	35.804714913746686	37.0	34.0	39.0	30.0	40.0
20	36.10254135212233	37.0	34.0	39.0	31.0	40.0
21	35.99027166177859	37.0	34.0	39.0	30.0	40.0
22	36.48424249864175	38.0	35.0	40.0	31.0	41.0
23	36.68103107744628	38.0	35.0	40.0	32.0	41.0
24	36.69339447779462	38.0	35.0	40.0	32.0	41.0
25	35.818591530122724	38.0	34.0	40.0	29.0	41.0
26	36.11768202044118	38.0	35.0	40.0	30.0	41.0
27	36.359334719739024	38.0	35.0	40.0	31.0	41.0
28	36.21726257397439	38.0	35.0	40.0	31.0	41.0
29	36.32131835755897	38.0	35.0	40.0	31.0	41.0
30	35.57479480426034	38.0	35.0	40.0	29.0	41.0
31	35.41651726950195	38.0	34.0	40.0	29.0	41.0
32	35.128517619547104	38.0	34.0	40.0	26.0	41.0
33	34.870499093893535	38.0	34.0	40.0	24.0	41.0
34	34.49599939714446	38.0	34.0	40.0	21.0	41.0
35	34.28184347390644	38.0	34.0	40.0	20.0	41.0
36	33.87398617564731	38.0	33.0	40.0	17.0	41.0
37	33.68237291720095	38.0	33.0	40.0	16.0	41.0
38	33.56673039595211	37.0	33.0	40.0	16.0	41.0
39	33.48177273561503	37.0	33.0	40.0	15.0	41.0
40	33.36094152422786	37.0	33.0	40.0	15.0	41.0
41	33.080030288629246	37.0	32.0	40.0	15.0	40.0
42	33.181888566528634	37.0	33.0	40.0	15.0	41.0
43	33.08462341583298	37.0	33.0	40.0	15.0	41.0
44	33.08140859142073	37.0	32.0	40.0	15.0	41.0
45	33.237076588177956	37.0	33.0	40.0	15.0	41.0
46	32.94888611499712	37.0	32.0	40.0	14.0	40.0
47	32.868506996649046	37.0	31.0	40.0	15.0	41.0
48	32.932599291887826	37.0	32.0	40.0	15.0	40.0
49	32.999251292310795	37.0	32.0	40.0	13.0	41.0
50	32.777705526945574	36.0	31.0	40.0	12.0	40.0
51	30.540480655751253	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	14.0
10	20.0
11	29.0
12	26.0
13	32.0
14	53.0
15	103.0
16	252.0
17	490.0
18	1239.0
19	2450.0
20	4142.0
21	6367.0
22	8085.0
23	9495.0
24	11231.0
25	14793.0
26	17913.0
27	18769.0
28	17755.0
29	17525.0
30	20898.0
31	27579.0
32	38142.0
33	54790.0
34	75475.0
35	101969.0
36	136181.0
37	142224.0
38	81745.0
39	12960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.40214840212896	23.921939930793158	25.795532305642897	17.88037936143499
2	35.7565047019086	24.182286013629884	26.585686313356042	13.475522971105475
3	26.282678477449434	23.30863165161756	37.058113572636195	13.350576298296811
4	23.662201565236625	27.3356094371201	34.75656668907118	14.245622308572095
5	19.92425411819615	29.211511137634595	34.489778803064354	16.374455941104905
6	17.506754777569398	35.59789049177698	33.28236611076741	13.61298861988621
7	69.71489551516802	6.733872094959471	18.784054957089083	4.76717743278343
8	64.02374472957189	6.756722264694908	18.72984657569544	10.489686430037763
9	57.66775123943939	8.673948740262851	24.365695088793572	9.292604931504185
10	29.227919504199935	29.323209573734943	26.280976869064883	15.16789405300024
11	20.53646850626739	25.723578579667482	35.64602170036864	18.09393121369649
12	20.66177980944417	18.128571098667763	41.10162126816011	20.108027823727955
13	21.023250047705808	23.46505807954047	41.288312016636866	14.223379856116857
14	13.885732135238973	31.107102878027497	34.494275910937816	20.512889075795716
15	10.131740952001273	24.208417856678388	48.37247235190233	17.287368839418
16	10.748938621770135	27.1558466656376	40.169869134160884	21.925345578431386
17	10.891144465336414	26.1172578337796	35.39649298511943	27.595104715764553
18	13.566194389310981	24.15080625851564	40.49086540156135	21.792133950612033
19	15.193904352592705	28.92004993005174	37.02590455678571	18.860141160569842
20	17.838082238733225	27.140532190176614	35.913052673287545	19.108332897802615
21	14.514111802963473	31.978934088199225	36.89305755933447	16.613896549502826
22	12.275524429626946	26.337859206491395	33.12800592159718	28.258610442284482
23	12.361334109590873	30.287292267040694	34.48455243445465	22.86682118891378
24	15.231096650140808	24.455637246262842	35.04705554900571	25.266210554590636
25	13.632435572852538	28.909718736288383	33.08522262507125	24.372623065787828
26	14.179867298854695	27.582950370160596	37.130188842067646	21.106993488917063
27	17.97870801737099	27.996076577239045	33.635692937474396	20.389522467915565
28	13.61335325025433	27.43138568047927	37.60773308084706	21.34752798841934
29	18.398154484163495	24.436554923664634	32.13292964700134	25.032360945170534
30	21.678976993039207	25.82482427854843	31.84633017766007	20.64986855075229
31	18.00338133894702	33.04632871913859	26.49173322183747	22.458556720076913
32	22.378338039090806	28.781976563990806	25.06031594005963	23.77936945685876
33	20.953241017027022	26.66444647286968	29.13724808599443	23.245064424108875
34	20.985206945965427	28.22919692592291	29.110386982209686	21.67520914590198
35	20.201616284878416	25.922788304116313	27.23181132566232	26.643784085342954
36	27.03770642636715	26.236248877242325	29.650647644305504	17.075397052085016
37	21.067734952616284	29.880243232764226	29.569699702583165	19.482322112036325
38	21.656612997127926	32.01211545169802	24.61473763021862	21.716533920955428
39	28.482736575221423	24.056853166997065	24.429262316302257	23.031147941479258
40	27.75712214266528	25.46906658272065	29.009992087521013	17.76381918709306
41	22.158344383659212	27.288815206544868	26.01795683019528	24.534883579600635
42	28.372739747505626	24.127591458412084	28.587020860503358	18.91264793357893
43	24.775539622558952	20.610974644819635	33.0404946332487	21.572991099372715
44	22.33385313418033	24.75135247480708	27.577116284270698	25.337678106741894
45	20.58581514941945	20.709667931123754	29.883281819165212	28.82123510029158
46	27.071981680970307	23.62269994202377	28.73141448627835	20.57390389072757
47	18.695571321092284	24.695928658853042	32.87081996861748	23.73768005143719
48	19.20848470557921	22.733974191462544	31.598989244619574	26.45855185833867
49	24.766545406812025	17.486092390042675	33.08595188580749	24.661410317337808
50	19.900310057356357	17.147593864972514	35.692208213663676	27.25988786400746
51	18.586182210656688	17.293932186044138	28.92284542954065	35.19704017375852
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1630.0
1	1537.0
2	1444.0
3	9751.5
4	18059.0
5	12379.0
6	6699.0
7	6850.5
8	7002.0
9	7356.5
10	7711.0
11	7724.0
12	7737.0
13	7821.0
14	7905.0
15	7714.5
16	7524.0
17	7457.0
18	7390.0
19	7253.5
20	7117.0
21	6971.0
22	6825.0
23	6457.5
24	6090.0
25	6367.0
26	7691.0
27	8738.0
28	10074.5
29	11411.0
30	11804.0
31	12197.0
32	12572.0
33	12947.0
34	14518.5
35	16090.0
36	15452.0
37	14814.0
38	16365.5
39	17917.0
40	22852.5
41	27788.0
42	35466.0
43	43144.0
44	54658.0
45	66172.0
46	87605.5
47	109039.0
48	118693.5
49	128348.0
50	117930.5
51	107513.0
52	84368.0
53	61223.0
54	47013.5
55	32804.0
56	26211.0
57	19618.0
58	15704.0
59	11790.0
60	9869.5
61	7949.0
62	6835.0
63	5721.0
64	4498.0
65	3275.0
66	2546.5
67	1818.0
68	1386.5
69	955.0
70	890.5
71	826.0
72	659.5
73	493.0
74	337.0
75	132.5
76	84.0
77	61.0
78	38.0
79	44.0
80	50.0
81	32.5
82	15.0
83	10.0
84	5.0
85	5.5
86	6.0
87	4.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	822751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.54770938217997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52977195589511	26.49362673808306
2	10.322760366646055	7.339009710708389
3	4.346249005915276	4.634975896945944
4	2.359537155546932	3.3550456432735167
5	1.461343119684057	2.597370026308855
6	1.0187510255780199	2.1728559234027145
7	0.7232761452171822	1.799756714927065
8	0.5658500799868199	1.609173935780381
9	0.4364996497539658	1.396490642538959
>10	3.8294921670453426	28.823939412459744
>50	0.30727146546117123	7.11357874658425
>100	0.08952002295106669	6.123400248743192
>500	0.005184557313768222	1.1559241851634343
>1k	0.003456371542512148	2.4954103104452967
>5k	6.912743085024295E-4	1.492823006987216
>10k+	3.4563715425121476E-4	1.3966188576480398
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11367	1.381584464801623	No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT	6112	0.7428736033137608	No Hit
GCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC	6038	0.7338793875668337	No Hit
CCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC	3301	0.40121494838657135	No Hit
TCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC	3027	0.3679120414317333	No Hit
ACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC	2287	0.2779698839624625	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC	2251	0.27359431954503854	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCG	2223	0.270191102775931	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC	1966	0.23895443457376533	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC	1538	0.18693383538883576	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1412	0.1716193599278518	No Hit
AGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTG	1194	0.1451228865112288	No Hit
AAAACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC	1111	0.13503477965994573	No Hit
ACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTG	912	0.11084763190807424	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.215434560395551E-4	0.0	0.0	0.8607707556721292	0.0
2	1.215434560395551E-4	0.0	0.0	2.882524603434089	0.0
3	1.215434560395551E-4	0.0	0.0	3.811602781400448	0.0
4	1.215434560395551E-4	0.0	0.0	5.312421376576874	0.0
5	1.215434560395551E-4	0.0	0.0	8.907555262770876	0.0
6	1.215434560395551E-4	0.0	0.0	11.459117035409255	0.0
7	1.215434560395551E-4	0.0	0.0	13.347051538071664	0.0
8	1.215434560395551E-4	0.0	0.0	18.187884305215064	0.0
9	1.215434560395551E-4	0.0	0.0	20.03279242443947	0.0
10	1.215434560395551E-4	0.0	0.0	24.127713001868123	0.0
11	1.215434560395551E-4	0.0	0.0	28.247428444328843	0.0
12	1.215434560395551E-4	0.0	0.0	32.05040164035048	0.0
13	1.215434560395551E-4	0.0	0.0	33.309834931832356	0.0
14	1.215434560395551E-4	0.0	0.0	33.71165759749912	0.0
15	1.215434560395551E-4	0.0	0.0	34.513115146623946	0.0
16	1.215434560395551E-4	0.0	0.0	36.17898975510209	0.0
17	1.215434560395551E-4	0.0	0.0	38.08333262432984	0.0
18	2.430869120791102E-4	0.0	0.0	41.82298167975487	0.0
19	2.430869120791102E-4	0.0	0.0	42.969865730944115	0.0
20	2.430869120791102E-4	0.0	0.0	44.27402701424854	0.0
21	2.430869120791102E-4	0.0	0.0	45.25731357360854	0.0
22	2.430869120791102E-4	0.0	0.0	46.14616086762581	0.0
23	2.430869120791102E-4	0.0	0.0	47.103072497025224	0.0
24	2.430869120791102E-4	0.0	0.0	47.79392550115406	0.0
25	2.430869120791102E-4	0.0	0.0	48.33187683758513	0.0
26	2.430869120791102E-4	0.0	0.0	48.83044809425938	0.0
27	2.430869120791102E-4	0.0	0.0	49.31443413620889	0.0
28	2.430869120791102E-4	0.0	0.0	49.8426619961568	0.0
29	2.430869120791102E-4	0.0	0.0	50.33357601510056	0.0
30	2.430869120791102E-4	0.0	0.0	50.91601225644211	0.0
31	2.430869120791102E-4	0.0	0.0	51.43840603050011	0.0
32	2.430869120791102E-4	0.0	0.0	51.92178435516943	0.0
33	2.430869120791102E-4	0.0	0.0	52.40042248505319	0.0
34	3.646303681186653E-4	0.0	0.0	52.83980207863618	0.0
35	3.646303681186653E-4	0.0	0.0	53.3376440745742	0.0
36	3.646303681186653E-4	0.0	0.0	53.7478532387077	0.0
37	3.646303681186653E-4	0.0	0.0	54.18577431081822	0.0
38	3.646303681186653E-4	0.0	0.0	54.59379569274301	0.0
39	3.646303681186653E-4	0.0	0.0	54.98674568611889	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	30	2.1647465E-6	45.000004	25
GTCGATG	30	2.1647465E-6	45.000004	1
GTTAGCG	30	2.1647465E-6	45.000004	1
ATCCGAG	30	2.1647465E-6	45.000004	31
GTCCCGA	30	2.1647465E-6	45.000004	38
CGACTGG	30	2.1647465E-6	45.000004	2
GTCGACC	20	7.032116E-4	45.0	10
CTCCGCC	25	3.889836E-5	45.0	45
GCACGAG	20	7.032116E-4	45.0	1
CACGATA	20	7.032116E-4	45.0	19
CACGACA	25	3.889836E-5	45.0	34
AGTATCG	20	7.032116E-4	45.0	1
ATATCCG	20	7.032116E-4	45.0	12
GCATTAT	20	7.032116E-4	45.0	9
TACGCGG	20	7.032116E-4	45.0	2
CACTAGG	25	3.889836E-5	45.0	2
GGAACGA	20	7.032116E-4	45.0	8
ACTACGG	25	3.889836E-5	45.0	2
CGAATTA	20	7.032116E-4	45.0	15
AGCGATC	20	7.032116E-4	45.0	38
>>END_MODULE