FastQCFastQC Report
Sat 18 Jun 2016
SRR3548237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502302
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC64511.2842871419982402No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG63211.2584062974067394No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC63151.2572117968871317Illumina PCR Primer Index 5 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC54021.0754486344868226No Hit
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT41180.8198255232907693Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31030.617755852057129No Hit
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC23000.4578918658496283Illumina PCR Primer Index 5 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC22840.4547065311306744Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTGAACT19850.3951805885702227No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT17690.35217856986434454No Hit
GAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT17610.3505859025048676Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC17330.3450115667466982No Hit
ACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC15880.31614447085617814Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTA12690.25263685989703405No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCC10990.21879267850814849No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9320.18554574737906676No Hit
TGAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATG7030.13995564421403855No Hit
AGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG6920.13776572659475772Illumina PCR Primer Index 5 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC6770.13477947529573842No Hit
TGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG5810.1156674669820148Illumina PCR Primer Index 5 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5770.11487113330227632No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCC5460.10869954728430306No Hit
ACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG5300.10551421256534912Illumina PCR Primer Index 5 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG5300.10551421256534912Illumina PCR Primer Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG302.1630512E-645.0000041
CCGGGAC302.1630512E-645.0000045
ACGCATG302.1630512E-645.0000041
CGCATGG453.8380676E-1045.0000042
GAGTAAG302.1630512E-645.0000041
TCGGGTA302.1630512E-645.00000411
ACGGGAT700.045.0000045
CTGCGCG302.1630512E-645.0000041
CACTCGA302.1630512E-645.00000441
GTCAATG302.1630512E-645.0000041
CGGGACG302.1630512E-645.0000046
CGTAAGG700.045.0000042
CAATGAG302.1630512E-645.0000041
TGATCCC502.1827873E-1145.04
TCACGAG207.0294907E-445.01
ACTATGC253.887659E-545.032
ACCGGTC253.887659E-545.017
CGAACCC207.0294907E-445.034
TCCGCAG207.0294907E-445.041
CTGTAGT253.887659E-545.030
Produced by FastQC (version 0.11.5)