##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548236_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1015172
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.486947039516455	28.0	25.0	31.0	16.0	33.0
2	27.38280311119692	30.0	25.0	31.0	16.0	33.0
3	27.015748070277745	30.0	25.0	31.0	16.0	33.0
4	29.60106563222784	32.0	28.0	35.0	19.0	37.0
5	31.68747956011395	33.0	32.0	35.0	26.0	37.0
6	31.009347184516514	33.0	30.0	35.0	17.0	36.0
7	32.18417765659415	35.0	32.0	35.0	26.0	35.0
8	32.9287805416225	35.0	32.0	35.0	28.0	37.0
9	34.1197186289614	37.0	32.0	38.0	27.0	39.0
10	34.2265428912539	37.0	32.0	39.0	27.0	39.0
11	34.666415149354	37.0	34.0	39.0	27.0	39.0
12	34.257683427044874	37.0	32.0	39.0	27.0	39.0
13	34.44660806247611	37.0	33.0	39.0	27.0	39.0
14	34.99441079935223	37.0	33.0	39.0	27.0	41.0
15	35.81880607424161	38.0	34.0	40.0	29.0	41.0
16	35.66758046912248	38.0	34.0	40.0	27.0	41.0
17	35.1152188988664	37.0	33.0	39.0	27.0	41.0
18	34.91153814329	37.0	33.0	39.0	27.0	40.0
19	34.29048969041699	36.0	32.0	38.0	27.0	40.0
20	34.107956090199494	35.0	32.0	38.0	27.0	40.0
21	34.14312254475104	35.0	32.0	38.0	27.0	40.0
22	34.42538111768252	35.0	33.0	39.0	27.0	40.0
23	34.79396397851793	35.0	33.0	39.0	28.0	40.0
24	34.3805049784667	35.0	33.0	39.0	25.0	40.0
25	33.41720417820822	35.0	32.0	39.0	23.0	40.0
26	33.332566304035176	35.0	31.0	38.0	22.0	40.0
27	33.908075675846064	35.0	33.0	39.0	24.0	40.0
28	33.78783890808651	35.0	33.0	39.0	23.0	40.0
29	33.7467000665897	35.0	33.0	39.0	23.0	40.0
30	32.98894965582187	35.0	31.0	39.0	20.0	40.0
31	32.79050939151198	35.0	31.0	39.0	18.0	40.0
32	32.19035789009153	35.0	31.0	39.0	15.0	40.0
33	31.807567584606353	35.0	30.0	39.0	12.0	40.0
34	31.32555074411036	35.0	29.0	39.0	10.0	40.0
35	31.032193559318028	35.0	27.0	39.0	8.0	40.0
36	30.5786359355853	35.0	25.0	39.0	7.0	40.0
37	30.44965582187058	35.0	25.0	39.0	7.0	40.0
38	30.33030264822119	35.0	24.0	39.0	7.0	40.0
39	30.09797551547915	35.0	24.0	39.0	7.0	40.0
40	29.97905576591947	35.0	23.0	39.0	7.0	40.0
41	29.68214154842726	35.0	23.0	39.0	7.0	40.0
42	29.75474993400133	35.0	23.0	39.0	7.0	40.0
43	29.64632692785065	35.0	23.0	39.0	7.0	40.0
44	29.49668430571371	35.0	22.0	39.0	7.0	40.0
45	29.631973695097972	35.0	23.0	39.0	7.0	40.0
46	29.3611683537371	35.0	22.0	39.0	7.0	40.0
47	29.412104549770877	35.0	22.0	39.0	7.0	40.0
48	29.24802102500857	35.0	22.0	38.0	7.0	40.0
49	29.281495155500743	35.0	22.0	39.0	7.0	40.0
50	29.128296485718675	35.0	21.0	39.0	7.0	40.0
51	27.0387569791129	32.0	18.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	13.0
9	49.0
10	56.0
11	56.0
12	47.0
13	81.0
14	206.0
15	556.0
16	1402.0
17	3113.0
18	5937.0
19	9567.0
20	13957.0
21	18341.0
22	23228.0
23	28279.0
24	33524.0
25	38510.0
26	40139.0
27	37435.0
28	35575.0
29	36556.0
30	41931.0
31	50079.0
32	61082.0
33	74493.0
34	86440.0
35	95079.0
36	109344.0
37	106757.0
38	58315.0
39	5021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.669187093418653	21.05574227815582	20.9241389636436	27.350931664781932
2	41.013936554593705	25.3167936073887	21.757396776112817	11.91187306190478
3	22.432257784887685	26.321155429818788	40.97758803434295	10.268998750950578
4	20.529624536531742	25.567687052046352	41.7115523280784	12.191136083343512
5	19.572742353019983	26.765021099872733	40.28401098533057	13.378225561776723
6	16.254880946282995	34.76543876308645	39.02609607041959	9.95358422021096
7	59.00468098016888	7.90732998940081	29.60266831630502	3.485320714125291
8	54.81839530641113	10.835503737297719	27.446186459043393	6.899914497247757
9	50.09082204788942	9.439188630104061	29.908921837875752	10.561067484130767
10	28.090116748688892	25.848821677508838	33.417982371460205	12.643079202342067
11	22.36054579913552	24.097689849601842	39.01407840247761	14.527685948785033
12	19.562497783626814	19.387453554668568	44.36154661476085	16.688502046943768
13	18.115255345892127	24.905730260487875	45.11373442135914	11.86527997226086
14	13.244356621341014	31.062913476731037	39.64599102418113	16.046738877746826
15	9.866111358469304	23.73981945916554	52.60822796531032	13.785841217054845
16	11.007297285583133	25.91570689498922	44.206991524588936	18.870004294838708
17	11.201944104053304	24.445808198019645	41.327380975834636	23.024866722092412
18	13.81716595808395	23.378501377106538	44.18876801172609	18.615564653083418
19	14.590729452742984	26.347554897101183	41.51562493843407	17.546090711721757
20	17.503142324650405	24.90986749043512	42.29903898058654	15.287951204327937
21	14.170111074773537	29.57193460812552	42.10872640301348	14.149227914087465
22	12.742372721075837	24.07523060131682	40.11320249179449	23.069194185812847
23	11.860453203989078	28.60007959242375	40.8232299551209	18.716237248466268
24	14.928997253667358	23.462034019850822	41.15539041659935	20.453578309882463
25	11.653394695677186	28.506696402186034	39.441493658217524	20.398415243919256
26	11.860354698514143	26.902534742881006	41.298814388103686	19.938296170501157
27	15.432852757956287	28.801917310564125	38.21165280366283	17.553577127816762
28	10.684297833273574	25.463566765040802	45.05049390645132	18.801641495234307
29	12.737053425429384	23.858124534561632	39.95992797279673	23.444894067212253
30	14.330576493441505	26.734188886218295	39.05535219647508	19.87988242386512
31	15.80382437655885	26.59992592388285	37.66691752727617	19.929332172282134
32	15.771810097205202	25.776420153432127	36.42377843360534	22.02799131575733
33	15.382516460264862	25.931861792878447	34.5763082512126	24.109313495644088
34	15.124826137836742	21.41755288758949	40.403695137375735	23.05392583719803
35	14.932641956239928	23.453168527106737	34.916447656160734	26.697741860492606
36	19.374253821027374	22.085420007644025	35.512208768563355	23.028117402765247
37	17.364052594043176	24.461175052109397	40.074095818245574	18.10067653560185
38	17.48491881178756	24.10428971642244	34.618074572584746	23.792716899205256
39	21.234529715161568	20.012569298601615	37.139420709003005	21.61348027723381
40	19.652827304141564	20.212239896293436	35.987005157746665	24.14792764181833
41	18.025812374651785	22.052223662591167	33.133006032475286	26.788957930281764
42	20.990728664699184	21.186459043393633	34.30256153637019	23.520250755536992
43	22.4644690751912	21.485915687193895	34.49435169606726	21.55526354154764
44	18.21395783177629	23.124554262725923	34.60418530061901	24.05730260487878
45	16.1836614879055	21.530834183763933	36.352361964277975	25.933142364052593
46	21.85550822914738	21.05485572888141	35.22565634197949	21.863979699991727
47	16.037971890477674	20.667138179540018	40.926069670952316	22.36882025903
48	16.434554932563152	21.479709842273035	35.40188263663694	26.683852588526868
49	19.408041198929837	18.414219462317718	39.1358311694964	23.041908169256047
50	17.97754469193398	18.533214076038345	38.52273309350534	24.96650813852234
51	16.262859889752672	19.332881521554967	32.81266622798895	31.59159236070341
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	269.0
1	688.5
2	1108.0
3	17222.5
4	33337.0
5	27202.5
6	21068.0
7	20875.5
8	20683.0
9	21154.5
10	21626.0
11	21655.5
12	21685.0
13	21239.0
14	20793.0
15	20236.5
16	19680.0
17	19248.0
18	18816.0
19	17905.5
20	16995.0
21	15844.0
22	14693.0
23	13799.0
24	12905.0
25	12450.5
26	11883.0
27	11770.0
28	12363.0
29	12956.0
30	13332.0
31	13708.0
32	14737.0
33	15766.0
34	17091.0
35	18416.0
36	20371.5
37	22327.0
38	26531.5
39	30736.0
40	33613.5
41	36491.0
42	46347.0
43	56203.0
44	69440.0
45	82677.0
46	114178.5
47	145680.0
48	127657.5
49	109635.0
50	97919.0
51	86203.0
52	68391.0
53	50579.0
54	40168.0
55	29757.0
56	24423.0
57	19089.0
58	16365.0
59	13641.0
60	11470.0
61	9299.0
62	7669.0
63	6039.0
64	4700.5
65	3362.0
66	2742.0
67	2122.0
68	1750.5
69	1379.0
70	1060.5
71	742.0
72	571.0
73	400.0
74	325.5
75	196.5
76	142.0
77	115.0
78	88.0
79	60.5
80	33.0
81	21.5
82	10.0
83	9.0
84	8.0
85	6.0
86	4.0
87	3.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1015172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.937939947498734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.05338985625617	35.66141466084128
2	7.703944248478803	6.615829910001223
3	2.8635419715583024	3.6886377963573755
4	1.4813034528962092	2.5441647481791985
5	0.8610039294190044	1.8484867507976823
6	0.5772950415530538	1.4872715895716286
7	0.4101375110064587	1.2327321872468344
8	0.2865049463314125	0.984154574419163
9	0.22957987804248045	0.8871918314887898
>10	2.1040058276988707	20.43946861571979
>50	0.3537273955280718	10.075231120137712
>100	0.06768483079872205	4.8538309397068895
>500	0.0034769604849327175	1.0401777218433421
>1k	0.0032451631192705367	3.0448911151424136
>5k	2.3179736566218117E-4	0.6016528876225244
>10k+	9.271894626487247E-4	4.9948635509241335
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16949	1.6695692946613974	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	12289	1.2105337814675738	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	10691	1.0531220325225676	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG	10256	1.0102721509261483	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	6045	0.5954655959778244	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	4731	0.46602940191415837	No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	3922	0.38633847269231225	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTC	2729	0.2688214410956961	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2691	0.26507823304819284	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	2360	0.23247292084494056	No Hit
AGAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	1956	0.19267670897148462	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTA	1925	0.18962303924852142	No Hit
AGAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	1796	0.17691583298199712	No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	1676	0.16509517598988152	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	1664	0.16391311029066996	No Hit
ACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	1553	0.152979002572963	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATTCTCGT	1413	0.13918823608216144	No Hit
AAAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	1103	0.10865153885252943	No Hit
AGAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATG	1074	0.10579488007943481	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5890627401070951	0.0
2	0.0	0.0	0.0	2.0987576489501287	0.0
3	0.0	0.0	0.0	2.607538426985772	0.0
4	0.0	0.0	0.0	3.8332420515932277	0.0
5	0.0	0.0	0.0	7.130318803119077	0.0
6	0.0	0.0	0.0	8.571158384983038	0.0
7	0.0	0.0	0.0	9.844144637558955	0.0
8	0.0	0.0	0.0	12.823245715996896	0.0
9	0.0	0.0	0.0	13.761116342846336	0.0
10	0.0	0.0	0.0	16.672544160004414	0.0
11	0.0	0.0	0.0	19.062976520234994	0.0
12	0.0	0.0	0.0	22.40211510955779	0.0
13	0.0	0.0	0.0	23.51286284491692	0.0
14	0.0	0.0	0.0	23.849554558242346	0.0
15	0.0	0.0	0.0	24.54165402513072	0.0
16	0.0	0.0	0.0	25.6680641310044	0.0
17	0.0	0.0	0.0	26.794769753302887	0.0
18	1.970109498685937E-4	0.0	0.0	29.036458846382683	0.0
19	1.970109498685937E-4	0.0	0.0	29.863116792031303	0.0
20	1.970109498685937E-4	0.0	0.0	30.800888913405807	0.0
21	1.970109498685937E-4	0.0	0.0	31.42915683253675	0.0
22	1.970109498685937E-4	0.0	0.0	32.0160524521953	0.0
23	1.970109498685937E-4	0.0	0.0	32.56738759540255	0.0
24	1.970109498685937E-4	0.0	0.0	33.01736060490242	0.0
25	1.970109498685937E-4	0.0	0.0	33.381141323834775	0.0
26	1.970109498685937E-4	0.0	0.0	33.71862108095968	0.0
27	1.970109498685937E-4	0.0	0.0	34.03866536902121	0.0
28	1.970109498685937E-4	0.0	0.0	34.359497700882216	0.0
29	1.970109498685937E-4	0.0	0.0	34.71756510226838	0.0
30	1.970109498685937E-4	0.0	0.0	35.16182479422206	0.0
31	1.970109498685937E-4	0.0	0.0	35.552990035186156	0.0
32	1.970109498685937E-4	0.0	0.0	35.89539506605777	0.0
33	2.9551642480289057E-4	0.0	0.0	36.23977020642808	0.0
34	2.9551642480289057E-4	0.0	0.0	36.61566709877735	0.0
35	2.9551642480289057E-4	0.0	0.0	36.96762716071759	0.0
36	2.9551642480289057E-4	0.0	0.0	37.284519273581225	0.0
37	2.9551642480289057E-4	0.0	0.0	37.613035032487105	0.0
38	2.9551642480289057E-4	0.0	0.0	37.927760024902184	0.0
39	2.9551642480289057E-4	0.0	0.0	38.27499182404558	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	30	2.1652504E-6	45.000004	25
CAGGTCA	30	2.1652504E-6	45.000004	15
ATCGTCC	30	2.1652504E-6	45.000004	10
CTAACGG	60	0.0	45.000004	2
ATGCGAG	60	0.0	45.000004	1
CACGTGT	30	2.1652504E-6	45.000004	41
CGTCCAG	30	2.1652504E-6	45.000004	12
CTCACGT	25	3.8904836E-5	45.0	45
GTACCGC	25	3.8904836E-5	45.0	18
GCGACGC	20	7.0328976E-4	45.0	9
TCCGCAA	20	7.0328976E-4	45.0	32
CGACGGT	25	3.8904836E-5	45.0	28
ACGCATT	20	7.0328976E-4	45.0	38
CGCGGAC	55	1.8189894E-12	45.0	4
TTCCGAT	25	3.8904836E-5	45.0	28
TCTAGCG	20	7.0328976E-4	45.0	1
CTGCGTA	20	7.0328976E-4	45.0	5
GTTCGCA	20	7.0328976E-4	45.0	32
ACGTGTG	25	3.8904836E-5	45.0	42
CCGAGTA	20	7.0328976E-4	45.0	17
>>END_MODULE