SRR3548232_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	925979
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	50672	5.472262329923249	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	47615	5.142125253380477	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	40078	4.328175908956899	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	12492	1.3490586719569235	TruSeq Adapter, Index 15 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	10682	1.1535898762282946	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	10245	1.1063965813479573	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	10235	1.105316643250009	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	7646	0.8257206696912134	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7206	0.7782033933814914	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	6133	0.6623260354716468	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCTGTGAGACT	2778	0.3000068036100171	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGT	2580	0.2786240292706422	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT	2549	0.27527622116700273	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	2179	0.23531851154291833	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTAT	2165	0.23380659820579083	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2037	0.21998339055205357	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTT	1831	0.1977366657343201	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1477	0.15950685706695292	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	1451	0.15669901801228753	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1357	0.1465475998915742	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGAGAC	1161	0.125380813171789	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	20	7.032575E-4	45.000004	26
TCGCCAC	20	7.032575E-4	45.000004	29
CTTCGGA	20	7.032575E-4	45.000004	12
CAGCGAA	40	6.8121153E-9	45.000004	14
TCGTTGC	40	6.8121153E-9	45.000004	17
CCTTACG	40	6.8121153E-9	45.000004	14
TCGTTAG	25	3.890215E-5	45.000004	1
GTACCGT	20	7.032575E-4	45.000004	43
ACACGTC	25	3.890215E-5	45.000004	10
CGAACTA	20	7.032575E-4	45.000004	34
GATAATC	20	7.032575E-4	45.000004	38
GTATTGA	20	7.032575E-4	45.000004	35
TACCAGC	20	7.032575E-4	45.000004	31
ACGTTGC	20	7.032575E-4	45.000004	28
GCGAAGT	40	6.8121153E-9	45.000004	16
TCACACG	25	3.890215E-5	45.000004	18
AGCTTAC	40	6.8121153E-9	45.000004	16
ATAGCGG	75	0.0	45.000004	2
ATTGTAC	20	7.032575E-4	45.000004	40
ATTCGCA	20	7.032575E-4	45.000004	33

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content