##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	925979
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.13479247369541	30.0	25.0	31.0	16.0	33.0
2	27.9822620167412	30.0	27.0	31.0	16.0	33.0
3	27.986506173466136	30.0	27.0	31.0	16.0	33.0
4	31.06178217864552	35.0	28.0	35.0	19.0	37.0
5	32.197527157743316	35.0	32.0	35.0	28.0	37.0
6	31.649320340958056	35.0	31.0	35.0	23.0	37.0
7	32.28205715248402	35.0	32.0	35.0	26.0	35.0
8	32.74638949695404	35.0	32.0	35.0	28.0	37.0
9	34.214173323585094	35.0	33.0	37.0	28.0	39.0
10	33.91663525846698	35.0	32.0	37.0	27.0	39.0
11	34.15866882510294	37.0	32.0	39.0	27.0	39.0
12	33.65126422953436	35.0	32.0	37.0	26.0	39.0
13	33.57053237708415	35.0	32.0	38.0	25.0	39.0
14	34.500843971623546	37.0	32.0	39.0	25.0	40.0
15	34.964290766853246	37.0	33.0	39.0	27.0	41.0
16	35.23696541714229	36.0	33.0	39.0	29.0	41.0
17	34.526234396244405	36.0	32.0	39.0	27.0	40.0
18	34.505617298016475	36.0	32.0	39.0	27.0	40.0
19	33.81900561459817	36.0	32.0	38.0	25.0	40.0
20	33.138209397837315	35.0	31.0	38.0	25.0	40.0
21	34.35683746607644	35.0	33.0	38.0	27.0	40.0
22	34.73562683386988	36.0	33.0	39.0	27.0	40.0
23	34.9378916800489	36.0	33.0	39.0	29.0	40.0
24	34.820073673377046	36.0	33.0	39.0	27.0	40.0
25	34.25318932718776	35.0	33.0	39.0	25.0	40.0
26	34.033594714350976	35.0	33.0	39.0	25.0	40.0
27	34.49080270718883	36.0	33.0	39.0	27.0	40.0
28	34.285255929130145	36.0	33.0	39.0	25.0	40.0
29	34.36203952789426	36.0	33.0	39.0	25.0	40.0
30	33.829572808886596	36.0	33.0	39.0	24.0	40.0
31	33.816451560996526	36.0	33.0	39.0	23.0	40.0
32	33.39880062074842	36.0	32.0	39.0	21.0	40.0
33	33.082486751859385	36.0	32.0	39.0	18.0	40.0
34	32.75005372692037	36.0	32.0	39.0	16.0	40.0
35	32.84052554107598	36.0	32.0	39.0	15.0	40.0
36	32.602710212650614	36.0	32.0	39.0	13.0	40.0
37	32.60486252927982	36.0	32.0	40.0	12.0	40.0
38	32.30873162350334	36.0	31.0	39.0	10.0	40.0
39	32.30487948430796	36.0	31.0	39.0	10.0	40.0
40	31.83158149374878	35.0	30.0	39.0	10.0	40.0
41	31.634629943011667	35.0	30.0	39.0	10.0	40.0
42	31.89732596527567	35.0	31.0	39.0	10.0	40.0
43	31.998717033539638	35.0	31.0	39.0	10.0	40.0
44	32.042826025212236	35.0	31.0	39.0	10.0	40.0
45	32.09502483317656	35.0	31.0	39.0	10.0	40.0
46	31.794569855255897	35.0	30.0	39.0	10.0	40.0
47	31.735384927735943	35.0	30.0	39.0	10.0	40.0
48	31.63655439270221	35.0	30.0	39.0	10.0	40.0
49	31.731772534798306	35.0	30.0	39.0	10.0	40.0
50	31.526367228630455	35.0	30.0	39.0	10.0	40.0
51	29.00985767495807	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	13.0
9	39.0
10	44.0
11	28.0
12	41.0
13	29.0
14	91.0
15	241.0
16	560.0
17	1185.0
18	2569.0
19	4410.0
20	6749.0
21	9443.0
22	12662.0
23	16773.0
24	21102.0
25	25532.0
26	27708.0
27	27406.0
28	28212.0
29	32318.0
30	40556.0
31	52968.0
32	68843.0
33	85752.0
34	97348.0
35	97577.0
36	106737.0
37	99977.0
38	54236.0
39	4824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.17566921064085	15.410392676291796	18.606361483359773	19.807576629707587
2	28.93208161308194	40.92986989985734	19.225382001103696	10.912666485957025
3	19.326356213261857	39.67379389813376	32.01130911176172	8.98854077684267
4	18.47752486827455	18.028594600957472	51.491556503981194	12.002324026786784
5	33.507779334088575	20.56731308161416	31.259888183209338	14.665019401087932
6	15.178745954281899	43.09687368720025	32.481406165798575	9.242974192719274
7	56.45452002691206	4.172880810471944	36.237970839511476	3.1346283231045198
8	51.07524036722215	20.326378891961912	21.65783457292228	6.940546167893656
9	48.842576343523994	4.448589006878126	21.51517475018332	25.193659899414566
10	39.21104042316295	17.35611714736511	28.307877392467862	15.12496503700408
11	34.37561758959976	17.35158140735373	32.84728919338343	15.42551180966307
12	26.79132032151917	13.96521951361748	37.059479750620696	22.183980414242654
13	19.091901652197297	24.42722783130071	44.87024003784103	11.610630478660964
14	16.28989426326083	33.71761130651991	30.694108613694265	19.298385816524995
15	9.67667733285528	18.23572672814394	58.26914001289446	13.818455926106315
16	10.185544164608483	20.97693360216592	38.87669158803818	29.96083064518742
17	11.559117431388833	21.66928191676053	41.4334450349306	25.338155616920037
18	13.654197341408391	21.71183147781969	40.781810386628635	23.85216079414328
19	15.632103967800564	25.619803472864934	33.894721154583415	24.85337140475108
20	18.100842459710208	21.300375062501416	44.495501517853	16.103280959935375
21	16.98343050976318	31.525660949114396	35.593679770275564	15.897228770846855
22	14.210581449471317	19.624095146866182	38.05907045408157	28.106252949580927
23	14.69342177306397	31.758603596841827	34.92811392051008	18.619860709584128
24	19.632194682600794	18.965224913307967	35.28308957330566	26.11949083078558
25	11.366348480905074	34.36913796101207	33.71728732509053	20.547226232992326
26	13.56326655356115	21.214951958953712	40.07779874057619	25.14398274690895
27	20.28328936185378	28.272671410474754	30.315482316553616	21.128556911117855
28	10.79203740041621	23.54427044241824	46.856462187587404	18.80722996957814
29	21.120565369193038	16.9809466521379	39.17669839164819	22.721789587020872
30	18.93099087560301	28.39934814936408	36.71130770784219	15.958353267190725
31	25.899615434043323	18.561328064675333	33.08455159350266	22.454504907778684
32	29.37885200420312	28.624515242786288	28.372565684534962	13.624067068475634
33	20.741615090623007	23.881103135168292	25.78471002042163	29.592571753787073
34	26.863244198842523	21.884945554920794	35.21904924409733	16.032761002139356
35	23.125794429463305	25.986982426167334	26.708921044645724	24.178302099723645
36	21.58882652846339	20.106071519980475	37.17784096615582	21.127260985400316
37	30.64227158499275	21.06484056333891	33.581431112368634	14.711456739299702
38	18.84189598252228	29.64462477010818	29.227336689060984	22.28614255830856
39	33.43607144438481	20.93600394825369	28.59751679033758	17.030407817023928
40	19.809304530664303	31.340019590077095	30.075088095950342	18.77558778330826
41	22.605372260062055	23.819222682155857	25.8666773220559	27.708727735726193
42	20.258342791791176	25.61861554095719	34.589553326803305	19.533488340448326
43	23.462627122213355	20.608134741716604	26.988085042965338	28.941153093104706
44	22.78453399051166	21.76064467984695	32.54242266833265	22.912398661308732
45	17.672107034824762	22.677728112624585	30.847999792651887	28.802165059898766
46	30.062020844965165	25.846266492004677	26.527383450380626	17.564329212649533
47	16.80275686597644	20.303916179524588	42.81036610981459	20.082960844684383
48	23.787040526837004	23.47904218130217	27.089167248933293	25.644750042927537
49	17.775565104608205	18.226655248121176	40.463444635353504	23.53433501191712
50	21.593470262284566	19.70422655373394	32.56974510221074	26.132558081770753
51	19.68057590938887	19.944188799098036	27.72492680719541	32.650308484317684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	96.0
1	326.5
2	557.0
3	9040.5
4	17524.0
5	14615.0
6	11706.0
7	11923.5
8	12141.0
9	12652.0
10	13163.0
11	12926.0
12	12689.0
13	12283.0
14	11877.0
15	11193.5
16	10510.0
17	9799.0
18	9088.0
19	8227.5
20	7367.0
21	7040.5
22	6714.0
23	6597.0
24	6480.0
25	6424.0
26	6287.5
27	6207.0
28	6329.5
29	6452.0
30	8132.5
31	9813.0
32	10867.5
33	11922.0
34	13376.0
35	14830.0
36	15140.0
37	15450.0
38	18275.5
39	21101.0
40	27041.5
41	32982.0
42	38815.0
43	44648.0
44	54877.0
45	65106.0
46	157068.5
47	249031.0
48	169256.5
49	89482.0
50	81112.5
51	72743.0
52	57403.5
53	42064.0
54	36092.0
55	30120.0
56	26630.5
57	23141.0
58	20379.0
59	17617.0
60	16785.5
61	15954.0
62	13955.0
63	11956.0
64	9925.0
65	7894.0
66	6375.0
67	4856.0
68	3757.5
69	2659.0
70	2167.5
71	1676.0
72	1333.0
73	990.0
74	782.0
75	348.0
76	122.0
77	170.5
78	219.0
79	131.5
80	44.0
81	35.0
82	26.0
83	15.5
84	5.0
85	4.5
86	4.0
87	4.0
88	4.0
89	4.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	925979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.943651566463267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.64078993137585	23.37229090586116
2	7.77075136886527	4.342863373223771
3	2.646146820715899	2.218290142553689
4	1.2734890916956767	1.4234374180814313
5	0.7590754123740401	1.06056694180248
6	0.4702613920801314	0.7884492291268296
7	0.3224042831757701	0.6306407066819352
8	0.2428276868797945	0.5428393818287378
9	0.16963072989420938	0.42660918100157696
>10	1.607400747297846	10.53053122223748
>50	0.6908729289914207	13.93401164321448
>100	0.3945599662289718	15.389172875546056
>500	0.0035368921275505437	0.6792070205245749
>1k	0.004322868155895109	2.368164817722305
>5k	0.001178964042516848	2.304472000922911
>10k+	0.0027509160992059785	19.988453139670593
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	50672	5.472262329923249	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	47615	5.142125253380477	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	40078	4.328175908956899	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	12492	1.3490586719569235	TruSeq Adapter, Index 15 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	10682	1.1535898762282946	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	10245	1.1063965813479573	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	10235	1.105316643250009	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	7646	0.8257206696912134	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7206	0.7782033933814914	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	6133	0.6623260354716468	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCTGTGAGACT	2778	0.3000068036100171	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGT	2580	0.2786240292706422	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT	2549	0.27527622116700273	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	2179	0.23531851154291833	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTAT	2165	0.23380659820579083	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2037	0.21998339055205357	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTT	1831	0.1977366657343201	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1477	0.15950685706695292	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	1451	0.15669901801228753	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1357	0.1465475998915742	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGAGAC	1161	0.125380813171789	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27052449353603053	0.0
2	0.0	0.0	0.0	2.1962701097973065	0.0
3	0.0	0.0	0.0	2.471006361915335	0.0
4	1.0799380979482256E-4	0.0	0.0	3.9943670428811022	0.0
5	1.0799380979482256E-4	0.0	0.0	9.510798841010434	0.0
6	1.0799380979482256E-4	0.0	0.0	10.290730135348642	0.0
7	1.0799380979482256E-4	0.0	0.0	11.916036972760722	0.0
8	1.0799380979482256E-4	0.0	0.0	14.080988877717528	0.0
9	1.0799380979482256E-4	0.0	0.0	14.559401455108594	0.0
10	1.0799380979482256E-4	0.0	0.0	20.80327955601585	0.0
11	1.0799380979482256E-4	0.0	0.0	21.55837227410125	0.0
12	1.0799380979482256E-4	0.0	0.0	28.338331646830003	0.0
13	1.0799380979482256E-4	0.0	0.0	29.39634700138988	0.0
14	1.0799380979482256E-4	0.0	0.0	29.880807232129456	0.0
15	1.0799380979482256E-4	0.0	0.0	30.84810778646168	0.0
16	1.0799380979482256E-4	0.0	0.0	31.55579122204715	0.0
17	1.0799380979482256E-4	0.0	0.0	32.00731334079931	0.0
18	2.159876195896451E-4	0.0	0.0	32.769857631760544	0.0
19	2.159876195896451E-4	0.0	0.0	33.98414002909353	0.0
20	2.159876195896451E-4	0.0	0.0	34.42875054401882	0.0
21	2.159876195896451E-4	0.0	0.0	34.677136306546906	0.0
22	2.159876195896451E-4	0.0	0.0	35.0090012840464	0.0
23	2.159876195896451E-4	0.0	0.0	35.21440551027615	0.0
24	2.159876195896451E-4	0.0	0.0	35.396051098351045	0.0
25	2.159876195896451E-4	0.0	0.0	35.54108678490549	0.0
26	2.159876195896451E-4	0.0	0.0	35.705669351032796	0.0
27	2.159876195896451E-4	0.0	0.0	35.92144098300285	0.0
28	2.159876195896451E-4	0.0	0.0	36.06194092954592	0.0
29	2.159876195896451E-4	0.0	0.0	36.211188374682365	0.0
30	2.159876195896451E-4	0.0	0.0	36.40384933135633	0.0
31	2.159876195896451E-4	0.0	0.0	36.563464182233076	0.0
32	2.159876195896451E-4	0.0	0.0	36.70936381926588	0.0
33	2.159876195896451E-4	0.0	0.0	36.851699660575456	0.0
34	2.159876195896451E-4	0.0	0.0	37.0385289515205	0.0
35	2.159876195896451E-4	0.0	0.0	37.209915127664885	0.0
36	2.159876195896451E-4	0.0	0.0	37.3576506594642	0.0
37	2.159876195896451E-4	0.0	0.0	37.5036582903068	0.0
38	2.159876195896451E-4	0.0	0.0	37.65214977877468	0.0
39	2.159876195896451E-4	0.0	0.0	37.80701290202045	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	20	7.032575E-4	45.000004	26
TCGCCAC	20	7.032575E-4	45.000004	29
CTTCGGA	20	7.032575E-4	45.000004	12
CAGCGAA	40	6.8121153E-9	45.000004	14
TCGTTGC	40	6.8121153E-9	45.000004	17
CCTTACG	40	6.8121153E-9	45.000004	14
TCGTTAG	25	3.890215E-5	45.000004	1
GTACCGT	20	7.032575E-4	45.000004	43
ACACGTC	25	3.890215E-5	45.000004	10
CGAACTA	20	7.032575E-4	45.000004	34
GATAATC	20	7.032575E-4	45.000004	38
GTATTGA	20	7.032575E-4	45.000004	35
TACCAGC	20	7.032575E-4	45.000004	31
ACGTTGC	20	7.032575E-4	45.000004	28
GCGAAGT	40	6.8121153E-9	45.000004	16
TCACACG	25	3.890215E-5	45.000004	18
AGCTTAC	40	6.8121153E-9	45.000004	16
ATAGCGG	75	0.0	45.000004	2
ATTGTAC	20	7.032575E-4	45.000004	40
ATTCGCA	20	7.032575E-4	45.000004	33
>>END_MODULE