SRR3548231_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	613414
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	20792	3.389554199936747	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	20784	3.388250023638195	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	17638	2.8753826942326066	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8208	1.3380848823143914	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	7304	1.190712960578011	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	6680	1.0889872092909518	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	5369	0.8752653183657366	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	5199	0.8475515720215059	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	4768	0.7772890739370147	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	3161	0.5153126599653741	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	2952	0.48124105416570206	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	2717	0.442930875395736	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	2034	0.33158682390685573	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT	1988	0.3240878101901815	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGT	1299	0.2117656264773872	No Hit
ACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1228	0.20019106182773788	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	1127	0.1837258360585184	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1026	0.16726061028929892	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1024	0.1669345662146609	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGAGAC	997	0.16253297120704777	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	964	0.1571532439755206	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTAT	831	0.13547131301209298	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCC	791	0.12895043151933278	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	759	0.12373372632512464	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	758	0.12357070428780563	No Hit
GAATGATCCGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	646	0.10531223610807708	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	30	2.1638389E-6	45.000004	26
TAGAATG	30	2.1638389E-6	45.000004	1
TTAGACG	30	2.1638389E-6	45.000004	1
TTACTCG	30	2.1638389E-6	45.000004	15
TTGCATC	30	2.1638389E-6	45.000004	17
TGTACGT	30	2.1638389E-6	45.000004	6
TATCCGA	30	2.1638389E-6	45.000004	30
CGGGTTA	20	7.0307107E-4	45.0	6
TATCAAG	20	7.0307107E-4	45.0	1
AAGTAGG	85	0.0	45.0	2
AGCGTCA	20	7.0307107E-4	45.0	34
CGGCGAT	45	3.8380676E-10	45.0	31
TCGCATC	25	3.8886712E-5	45.0	10
ATCTCAA	20	7.0307107E-4	45.0	10
CCGGGCT	45	3.8380676E-10	45.0	5
GATGTCG	20	7.0307107E-4	45.0	4
ATGATCC	530	0.0	45.0	3
ATTAGCG	25	3.8886712E-5	45.0	1
GAACTTC	20	7.0307107E-4	45.0	20
TAGGTCA	20	7.0307107E-4	45.0	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content