##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	613414
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.746595284750594	30.0	25.0	31.0	16.0	33.0
2	28.080122070901545	31.0	27.0	31.0	16.0	33.0
3	28.019750119821197	31.0	26.0	31.0	16.0	34.0
4	32.240009194442905	35.0	32.0	35.0	26.0	37.0
5	33.30475828722527	35.0	32.0	35.0	28.0	37.0
6	32.18358889754717	35.0	32.0	35.0	25.0	37.0
7	33.67891016507612	35.0	33.0	35.0	30.0	37.0
8	32.857332894260644	35.0	33.0	36.0	26.0	37.0
9	35.65915189415305	37.0	34.0	39.0	30.0	39.0
10	35.31442223359754	37.0	34.0	39.0	30.0	39.0
11	35.67920197452292	37.0	35.0	39.0	31.0	39.0
12	35.64735072887153	37.0	34.0	39.0	31.0	39.0
13	35.36282999735904	37.0	34.0	39.0	30.0	39.0
14	36.04174505309628	38.0	34.0	40.0	30.0	41.0
15	36.385623738616985	38.0	34.0	40.0	30.0	41.0
16	36.292772907041574	37.0	34.0	40.0	31.0	41.0
17	35.53566433110428	37.0	34.0	39.0	29.0	41.0
18	35.74981333976727	37.0	34.0	39.0	30.0	40.0
19	35.23397574884173	37.0	34.0	39.0	29.0	40.0
20	34.77209519182803	36.0	33.0	39.0	27.0	40.0
21	35.489025356447684	37.0	34.0	39.0	30.0	40.0
22	35.937484309128905	37.0	34.0	40.0	30.0	40.0
23	36.03627403352385	37.0	34.0	40.0	31.0	41.0
24	36.01706514686656	37.0	35.0	40.0	30.0	41.0
25	35.517153178766705	37.0	34.0	40.0	29.0	41.0
26	35.60868353183984	37.0	34.0	40.0	30.0	41.0
27	35.68289931432931	37.0	34.0	40.0	30.0	41.0
28	35.529919434509154	37.0	34.0	40.0	29.0	41.0
29	35.682749334054975	37.0	34.0	40.0	30.0	41.0
30	35.008061439745426	37.0	34.0	40.0	27.0	41.0
31	35.18269390656229	37.0	34.0	40.0	27.0	41.0
32	34.70480784592461	37.0	34.0	40.0	25.0	41.0
33	34.37254122012214	37.0	33.0	40.0	23.0	41.0
34	34.0097047018816	37.0	33.0	40.0	20.0	41.0
35	33.95189219678716	37.0	34.0	40.0	18.0	41.0
36	33.698125572614906	37.0	33.0	40.0	17.0	41.0
37	33.82557457117053	37.0	34.0	40.0	17.0	41.0
38	33.433976074885805	37.0	33.0	40.0	16.0	41.0
39	33.487965713205114	37.0	33.0	40.0	15.0	41.0
40	33.03077693042545	37.0	32.0	40.0	15.0	41.0
41	32.832579628114125	36.0	32.0	40.0	15.0	41.0
42	33.11465339884646	37.0	33.0	40.0	15.0	41.0
43	33.261431920366995	37.0	33.0	40.0	15.0	41.0
44	33.41242782199298	37.0	33.0	40.0	15.0	41.0
45	33.572698047322035	37.0	33.0	40.0	15.0	41.0
46	33.25133596559583	37.0	33.0	40.0	15.0	41.0
47	33.09329588173729	36.0	33.0	40.0	15.0	41.0
48	33.19264966238136	36.0	33.0	40.0	15.0	41.0
49	33.2978119182151	37.0	33.0	40.0	15.0	41.0
50	33.089887090936955	36.0	33.0	40.0	13.0	41.0
51	30.973629555243278	35.0	29.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	10.0
11	16.0
12	18.0
13	25.0
14	31.0
15	67.0
16	135.0
17	388.0
18	944.0
19	2121.0
20	3723.0
21	5048.0
22	6351.0
23	7425.0
24	8761.0
25	11687.0
26	13741.0
27	14023.0
28	13408.0
29	13970.0
30	17184.0
31	23460.0
32	33833.0
33	48856.0
34	63411.0
35	65409.0
36	82332.0
37	96384.0
38	68634.0
39	12008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.250864831907975	18.3662257463964	20.77357217148614	16.60933725020948
2	29.257402015604466	38.19296592513376	20.34645443371035	12.203177625551422
3	20.992184723530926	37.59353389391178	30.79192845288826	10.622352929669033
4	19.683606829971275	20.746510513291184	46.80101856168917	12.768864095048368
5	31.762561663085613	23.579018411708894	29.688595304313235	14.969824620892252
6	16.03028297365238	42.558043996387426	30.10364941132742	11.308023618632767
7	58.731460318805894	5.808964255788098	31.29990512117428	4.159670304231726
8	53.50823424310498	17.77461877296573	19.062818911860504	9.654328072068783
9	50.070588542159136	6.333732193917974	21.31888088631821	22.276798377604685
10	38.819785658625335	19.484067856292814	26.136019067057486	15.560127418024368
11	32.511158858454486	19.652306598806028	31.34098667457867	16.495547868160816
12	24.588939932900132	16.112609102498475	35.042075987832035	24.25637497676936
13	21.78936248602086	24.556335525436328	41.15116381432442	12.503138174218389
14	15.72363852145533	34.98387712050915	29.62746204031861	19.66502231771691
15	9.94304010016074	20.76020436442598	51.93490856093926	17.36184697447401
16	10.46405853143228	23.01952677963007	37.180110007270784	29.33630468166687
17	11.905662407444238	26.31876677089209	36.486614260515736	25.288956561147934
18	14.180960982305587	22.779884384771133	37.9908185988582	25.048336034065084
19	15.550998183934503	27.722223490171398	31.580792091474923	25.14598623441917
20	19.092162878577927	23.472075955227627	39.73401324391031	17.701747922284135
21	17.21137763402857	33.13455512916236	32.9573501745966	16.69671706221247
22	14.273394477465464	22.27810255390324	36.064550205896836	27.383952762734467
23	15.649463494475183	31.53710218547343	31.85662537861868	20.956808941432705
24	18.8869181335933	22.350158294398238	33.51879807112326	25.24412550088521
25	12.283221445875053	33.69388373920387	31.077543062271157	22.945351752649923
26	13.59913533111406	23.87311016703238	38.87733243779894	23.65042206405462
27	21.222697884299997	27.589197507719092	30.11750628449954	21.070598323481367
28	12.144815736191218	26.389844379163176	41.83341104050445	19.63192884414115
29	20.13664507168079	19.384135347416265	37.67765326516839	22.80156631573456
30	20.142187820949637	29.559481850756587	33.56346610934866	16.734864218945116
31	24.46748851509747	21.69660294678635	30.078544017580295	23.757364520535887
32	27.77324938785225	28.998196976267256	27.5549628798821	15.673590755998395
33	22.65892203308043	24.53628381484609	24.89705158343305	27.90774256864043
34	24.48069330012031	23.09989664402834	33.85234115947794	18.567068896373414
35	24.22181430485773	25.63277003785372	26.834894541044058	23.310521116244495
36	21.81707623236509	23.29992468381876	33.98797549452735	20.8950235892888
37	30.136742884903185	21.297361977392104	32.28586240287962	16.28003273482509
38	20.73591408086545	29.03422484651475	27.495622858297985	22.734238214321813
39	31.53710218547343	22.336953509375398	26.671383437613095	19.454560867538074
40	22.20376450488577	28.764912440863753	28.849194834157682	20.182128220092793
41	23.20488283606178	22.84982083878099	25.305095742842514	28.64020058231472
42	22.304186079874277	24.026024838037603	31.82695536782662	21.842833714261495
43	23.890716547062834	19.71360288483797	28.48043898574209	27.915241582357105
44	23.57624703707447	21.45663450785277	31.077543062271157	23.8895753928016
45	19.49124082593485	21.14086082156586	30.30905717834937	29.058841174149926
46	29.68566090764149	24.24806085286609	26.357239971699375	19.70903826779304
47	17.572308424652842	21.515159419250292	38.76191283537709	22.150619320719773
48	22.861395403430638	22.211589562677084	27.79819175956206	27.128823274330223
49	19.77098664197426	17.648276694043503	37.32992725956695	25.250809404415286
50	20.940180693626164	18.543756745036795	32.761886751851115	27.754175809485925
51	19.07080699168914	19.00429400046298	28.347576025327104	33.577322982520776
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1112.0
1	1122.0
2	1132.0
3	6866.5
4	12601.0
5	8537.5
6	4474.0
7	4709.0
8	4944.0
9	5178.0
10	5412.0
11	5602.0
12	5792.0
13	5636.5
14	5481.0
15	5310.5
16	5140.0
17	4915.5
18	4691.0
19	4281.5
20	3872.0
21	3887.0
22	3902.0
23	4066.5
24	4231.0
25	4289.0
26	4756.5
27	5166.0
28	5564.5
29	5963.0
30	7025.0
31	8087.0
32	8421.0
33	8755.0
34	9473.5
35	10192.0
36	9969.0
37	9746.0
38	11409.0
39	13072.0
40	16711.0
41	20350.0
42	24562.0
43	28774.0
44	35191.5
45	41609.0
46	86823.5
47	132038.0
48	106028.0
49	80018.0
50	70664.0
51	61310.0
52	48804.0
53	36298.0
54	30410.0
55	24522.0
56	21218.0
57	17914.0
58	15269.0
59	12624.0
60	11741.5
61	10859.0
62	9243.0
63	7627.0
64	6325.5
65	5024.0
66	3905.0
67	2786.0
68	2123.0
69	1460.0
70	1256.0
71	1052.0
72	781.5
73	511.0
74	383.5
75	163.5
76	71.0
77	107.0
78	143.0
79	84.5
80	26.0
81	22.5
82	19.0
83	10.0
84	1.0
85	2.5
86	4.0
87	4.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	613414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.231659765348756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.19494560397943	22.8576804526548
2	9.37029323972419	5.478184477703301
3	3.6852926914263993	3.2318166627450866
4	1.8080231119261831	2.11406065822853
5	1.1058039480443411	1.6162242388205783
6	0.7356876825564858	1.2903223218029454
7	0.5147459098428562	1.053281411149188
8	0.35814458075194067	0.8375328425075361
9	0.30073234646638475	0.7911815069106307
>10	3.041731295009042	21.084255624645568
>50	0.778605502974262	15.208611200989065
>100	0.09020773387102238	4.2983473167065345
>500	0.005074185030245008	1.1365132892146874
>1k	0.006201781703632787	3.843972731676823
>5k	0.0028189916834694493	5.399097354215946
>10k+	0.0016913950100816695	9.758917910028785
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	20792	3.389554199936747	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	20784	3.388250023638195	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	17638	2.8753826942326066	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8208	1.3380848823143914	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	7304	1.190712960578011	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	6680	1.0889872092909518	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	5369	0.8752653183657366	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	5199	0.8475515720215059	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	4768	0.7772890739370147	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	3161	0.5153126599653741	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	2952	0.48124105416570206	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	2717	0.442930875395736	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	2034	0.33158682390685573	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT	1988	0.3240878101901815	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGT	1299	0.2117656264773872	No Hit
ACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1228	0.20019106182773788	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	1127	0.1837258360585184	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1026	0.16726061028929892	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1024	0.1669345662146609	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGAGAC	997	0.16253297120704777	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	964	0.1571532439755206	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTAT	831	0.13547131301209298	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCC	791	0.12895043151933278	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	759	0.12373372632512464	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	758	0.12357070428780563	No Hit
GAATGATCCGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	646	0.10531223610807708	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.260440746380096E-4	0.0	0.0	0.5221595855327723	0.0
2	3.260440746380096E-4	0.0	0.0	2.6409570045678774	0.0
3	3.260440746380096E-4	0.0	0.0	3.2491922258050843	0.0
4	3.260440746380096E-4	0.0	0.0	4.798879712559544	0.0
5	3.260440746380096E-4	0.0	0.0	9.610149099955333	0.0
6	3.260440746380096E-4	0.0	0.0	11.237598098510956	0.0
7	3.260440746380096E-4	0.0	0.0	13.08806124411898	0.0
8	3.260440746380096E-4	0.0	0.0	16.250036679958395	0.0
9	3.260440746380096E-4	0.0	0.0	17.09481687734548	0.0
10	3.260440746380096E-4	0.0	0.0	22.448297560864276	0.0
11	3.260440746380096E-4	0.0	0.0	24.23029145079832	0.0
12	3.260440746380096E-4	0.0	0.0	29.82569683769852	0.0
13	3.260440746380096E-4	0.0	0.0	30.825674014613295	0.0
14	3.260440746380096E-4	0.0	0.0	31.263225162777506	0.0
15	3.260440746380096E-4	0.0	0.0	32.28798168936477	0.0
16	3.260440746380096E-4	0.0	0.0	33.30768453279514	0.0
17	3.260440746380096E-4	0.0	0.0	34.17903732226522	0.0
18	4.890661119570143E-4	0.0	0.0	35.435285141845476	0.0
19	4.890661119570143E-4	0.0	0.0	37.51577238211061	0.0
20	4.890661119570143E-4	0.0	0.0	38.26958628267369	0.0
21	4.890661119570143E-4	0.0	0.0	38.804787631191985	0.0
22	4.890661119570143E-4	0.0	0.0	39.413022852429194	0.0
23	4.890661119570143E-4	0.0	0.0	39.882526319907925	0.0
24	4.890661119570143E-4	0.0	0.0	40.260411402413375	0.0
25	4.890661119570143E-4	0.0	0.0	40.56738189868506	0.0
26	4.890661119570143E-4	0.0	0.0	40.88918740035278	0.0
27	4.890661119570143E-4	0.0	0.0	41.259084403029604	0.0
28	6.520881492760192E-4	0.0	0.0	41.548448519270835	0.0
29	6.520881492760192E-4	0.0	0.0	41.84074703218381	0.0
30	6.520881492760192E-4	0.0	0.0	42.198906448173666	0.0
31	6.520881492760192E-4	0.0	0.0	42.499845129064546	0.0
32	6.520881492760192E-4	0.0	0.0	42.76410385155865	0.0
33	6.520881492760192E-4	0.0	0.0	43.03162301479914	0.0
34	6.520881492760192E-4	0.0	0.0	43.32571477012262	0.0
35	6.520881492760192E-4	0.0	0.0	43.61067729135625	0.0
36	6.520881492760192E-4	0.0	0.0	43.86059007456628	0.0
37	6.520881492760192E-4	0.0	0.0	44.111970056112185	0.0
38	6.520881492760192E-4	0.0	0.0	44.363187015620774	0.0
39	6.520881492760192E-4	0.0	0.0	44.64766047074244	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	30	2.1638389E-6	45.000004	26
TAGAATG	30	2.1638389E-6	45.000004	1
TTAGACG	30	2.1638389E-6	45.000004	1
TTACTCG	30	2.1638389E-6	45.000004	15
TTGCATC	30	2.1638389E-6	45.000004	17
TGTACGT	30	2.1638389E-6	45.000004	6
TATCCGA	30	2.1638389E-6	45.000004	30
CGGGTTA	20	7.0307107E-4	45.0	6
TATCAAG	20	7.0307107E-4	45.0	1
AAGTAGG	85	0.0	45.0	2
AGCGTCA	20	7.0307107E-4	45.0	34
CGGCGAT	45	3.8380676E-10	45.0	31
TCGCATC	25	3.8886712E-5	45.0	10
ATCTCAA	20	7.0307107E-4	45.0	10
CCGGGCT	45	3.8380676E-10	45.0	5
GATGTCG	20	7.0307107E-4	45.0	4
ATGATCC	530	0.0	45.0	3
ATTAGCG	25	3.8886712E-5	45.0	1
GAACTTC	20	7.0307107E-4	45.0	20
TAGGTCA	20	7.0307107E-4	45.0	15
>>END_MODULE