SRR3548230_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548230_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	973461
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGC	41617	4.2751584295621505	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCG	33844	3.4766672727515533	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTC	32273	3.3152843308566036	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	10683	1.0974245501360609	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCT	8990	0.9235090054968818	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8525	0.8757412983160086	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTC	7744	0.795512095502542	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCT	6930	0.7118929263730134	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	6153	0.6320746285675544	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTA	5314	0.5458873031379788	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTCAGTT	4780	0.49103148456897605	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGCT	2826	0.29030438815730675	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGT	2102	0.2159305817079472	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTAT	1657	0.17021739956711157	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	1587	0.16302656192698015	Illumina Single End Adapter 2 (95% over 21bp)
TGCAAGGGGATCTCAGCAGTTCTAAAAAACCAAATTTGATTGGCTCTAAAC	1476	0.1516239479547717	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTT	1284	0.13190050757041114	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCCGTTCGTATGC	1220	0.12532602744229096	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATG	1216	0.12491512243428345	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGCTTCAGTT	1119	0.1149506759901013	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	1070	0.10991708964200929	Illumina Single End Adapter 2 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGT	45	3.8380676E-10	45.000004	35
TCATACG	95	0.0	45.000004	31
TGATCGC	20	7.032755E-4	45.0	15
TCCGGAA	25	3.8903636E-5	45.0	26
CTATGTA	20	7.032755E-4	45.0	18
CTTCGAA	25	3.8903636E-5	45.0	23
CTATGCG	25	3.8903636E-5	45.0	1
CATGCGA	20	7.032755E-4	45.0	10
ACGTTAG	25	3.8903636E-5	45.0	1
CCCTAGA	20	7.032755E-4	45.0	23
GGCGTAA	40	6.8139343E-9	45.0	8
ACTCTCA	100	0.0	45.0	27
GTCTCGC	20	7.032755E-4	45.0	31
GCACGTC	20	7.032755E-4	45.0	9
CCTACGT	20	7.032755E-4	45.0	29
ATTCGTG	20	7.032755E-4	45.0	1
AAGGTCG	20	7.032755E-4	45.0	40
GCGTTAG	70	0.0	45.0	1
CGTTCCG	20	7.032755E-4	45.0	20
TACATGA	20	7.032755E-4	45.0	26

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content