##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548230_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	973461
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.8478151667093	28.0	25.0	31.0	16.0	33.0
2	27.678709265188846	30.0	26.0	31.0	16.0	33.0
3	27.701671664298825	30.0	26.0	31.0	16.0	33.0
4	30.64661655680094	33.0	28.0	35.0	19.0	37.0
5	31.96052641040576	33.0	32.0	35.0	26.0	37.0
6	31.31464742809419	35.0	30.0	35.0	18.0	36.0
7	32.16464758218357	35.0	32.0	35.0	26.0	35.0
8	32.768153012806884	35.0	32.0	35.0	27.0	37.0
9	34.06919023977334	35.0	32.0	37.0	27.0	39.0
10	33.926431567366336	35.0	32.0	38.0	27.0	39.0
11	34.29867555043294	37.0	33.0	39.0	27.0	39.0
12	33.80356480639697	35.0	32.0	38.0	26.0	39.0
13	33.85132635000272	35.0	32.0	39.0	26.0	39.0
14	34.75738113802196	37.0	33.0	39.0	26.0	40.0
15	35.21286317582317	37.0	33.0	39.0	27.0	41.0
16	35.316636208332945	37.0	33.0	39.0	27.0	41.0
17	34.67573431293087	36.0	32.0	39.0	27.0	40.0
18	34.58254722068989	36.0	33.0	39.0	27.0	40.0
19	34.00620261109587	36.0	32.0	38.0	26.0	40.0
20	33.48489872732446	35.0	32.0	38.0	25.0	40.0
21	34.35653919366056	35.0	33.0	39.0	27.0	40.0
22	34.64911588651215	36.0	33.0	39.0	27.0	40.0
23	34.86808408349179	36.0	33.0	39.0	28.0	40.0
24	34.70273796279461	36.0	33.0	39.0	27.0	40.0
25	33.96342123618717	35.0	32.0	39.0	24.0	40.0
26	33.909078021615656	35.0	32.0	39.0	24.0	40.0
27	34.26370650698898	36.0	33.0	39.0	25.0	40.0
28	34.06771406353208	36.0	33.0	39.0	24.0	40.0
29	34.09366785110035	36.0	33.0	39.0	24.0	40.0
30	33.65086428732122	36.0	32.0	39.0	23.0	40.0
31	33.54408137562778	36.0	32.0	39.0	22.0	40.0
32	33.12138339389046	36.0	32.0	39.0	19.0	40.0
33	32.85967902155299	36.0	31.0	39.0	17.0	40.0
34	32.568928801462	36.0	31.0	39.0	15.0	40.0
35	32.474095007401424	36.0	31.0	39.0	12.0	40.0
36	32.14384037984059	36.0	31.0	39.0	10.0	40.0
37	32.190696905166206	36.0	31.0	40.0	10.0	40.0
38	32.01429949427866	36.0	30.0	39.0	10.0	40.0
39	31.852357721572822	36.0	30.0	39.0	10.0	40.0
40	31.811736679743717	36.0	30.0	39.0	10.0	40.0
41	31.07931493917065	35.0	29.0	39.0	9.0	40.0
42	31.524583932997828	35.0	30.0	39.0	10.0	40.0
43	31.40196679682083	35.0	29.0	39.0	10.0	40.0
44	31.491192764784618	35.0	30.0	39.0	10.0	40.0
45	31.5361437181356	35.0	30.0	39.0	10.0	40.0
46	31.349888696105957	35.0	29.0	39.0	10.0	40.0
47	31.37356709719239	35.0	29.0	39.0	10.0	40.0
48	31.20193515713521	35.0	29.0	39.0	10.0	40.0
49	31.348869651686098	35.0	29.0	39.0	10.0	40.0
50	31.16146409563403	35.0	29.0	39.0	10.0	40.0
51	28.885434547454906	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	44.0
10	68.0
11	52.0
12	33.0
13	58.0
14	127.0
15	325.0
16	785.0
17	1729.0
18	3416.0
19	5605.0
20	8320.0
21	11550.0
22	15145.0
23	19150.0
24	23915.0
25	28705.0
26	31403.0
27	31426.0
28	31895.0
29	35220.0
30	42217.0
31	53377.0
32	67237.0
33	83151.0
34	95664.0
35	102073.0
36	113320.0
37	106167.0
38	56690.0
39	4581.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.967537477104884	16.30132075142199	20.819734945724587	21.911406825748543
2	32.337915951435136	35.870569031527715	20.564357483247917	11.227157533789232
3	20.725124067630855	34.1248391050078	35.065708847093	10.084327980268341
4	20.091097640275265	20.2188890977656	47.48182002155197	12.208193240407166
5	29.064954836403306	23.048278256653322	33.37021205780201	14.516554849141365
6	16.268859255789394	41.292563338438825	32.9919739979311	9.446603407840684
7	58.67774877473263	5.34135419908964	32.93146823550199	3.0494287906757434
8	53.774213861674994	16.96996592570221	22.371723160968955	6.884097051653841
9	50.17026876269311	5.490409990744364	23.348341638750806	20.990979607811717
10	35.80492695649851	20.511556189718952	29.57909972767271	14.104417126109828
11	29.047285921058986	20.72296681633882	33.86566077120706	16.364086491395135
12	22.930656698111175	14.311718702649618	40.80687361897395	21.95075098026526
13	19.353317698397777	21.945101036405156	46.38634727020394	12.315233994993124
14	16.719108418313624	30.237780455508744	33.78070616080151	19.262404965376117
15	11.889433680445338	19.262815870384124	53.70795542913378	15.139795020036756
16	11.67771487506947	22.248657111070706	38.92667502858358	27.146952985276247
17	11.690042025309694	21.615349767479128	41.88046567864557	24.814142528565604
18	14.596064968190817	21.303575592653427	40.89665636322359	23.203703075932165
19	16.882032253988605	25.477137758985723	35.86892541149568	21.77190457552999
20	18.56746187058341	21.730608622225237	44.86517693056014	14.836752576631214
21	17.13833425273329	30.42186589909611	37.51028546598169	14.929514382188911
22	13.79058842624409	19.85164274685889	39.39336039142811	26.96440843546891
23	14.217929634571904	30.582016125967037	37.175192431951565	18.024861807509495
24	18.842870952200446	20.493168190610614	35.40624637247923	25.25771448470971
25	14.039699587348645	31.409784264598173	34.671548218161796	19.878967929891385
26	16.3293650182185	21.81340598133875	36.90522784169063	24.95200115875212
27	18.813696696631915	26.87904292005535	31.628385728858166	22.67887465445457
28	13.49401773671467	22.77584823634434	45.079052987228046	18.651081039712942
29	23.00770138711258	18.962649762034637	34.627581382304996	23.40206746854779
30	15.254437517270853	24.90361709405924	36.548767747244106	23.2931776414258
31	21.681813652524344	23.6049518162515	32.83850097744029	21.87473355378387
32	24.10019507715255	25.709607267265973	31.90307572671119	18.287121928870288
33	15.543714642908139	21.69475716027658	29.165421110861146	33.59610708595414
34	24.328966440360734	24.439499887514753	32.38753273115205	18.844000940972467
35	19.619275964830642	18.674810803925375	32.18177204839228	29.5241411828517
36	24.070199011568004	18.443676736921148	36.26123696789085	21.224887283619992
37	17.79876132685336	21.970885325657626	37.424303593056116	22.806049754432895
38	19.73515117708876	22.591557340252972	31.713134886759715	25.960156595898553
39	20.51248072598697	23.91343875101314	36.85715195575375	18.716928567246146
40	23.04591555285728	19.082839476876835	32.87517424940496	24.996070720860928
41	16.42551679009226	27.383736996140573	32.41095431660847	23.779791897158695
42	25.52747362246664	20.073634177434947	34.8305684562607	19.56832374383771
43	22.58364741884883	19.13790074794984	34.596660780452424	23.681791052748906
44	20.532409618875334	20.834116621004846	34.651824777777435	23.981648982342385
45	18.41778972141668	20.237174370621936	32.639622953564654	28.705412954396735
46	27.227490366845718	23.786263651034815	29.47113443681873	19.515111545300737
47	15.948456075795539	20.09592577411935	43.27466637081506	20.68095177927005
48	20.161670575400557	23.078479774741876	30.18097283815171	26.57887681170586
49	18.216446267492998	17.59803423044169	41.72658175314676	22.45893774891855
50	21.554330373790012	17.603067816789782	36.36848317498082	24.47411863443939
51	18.44963485953726	19.94964359126868	28.861762309943593	32.73895923925047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	383.5
2	648.0
3	11602.5
4	22557.0
5	18955.5
6	15354.0
7	15072.5
8	14791.0
9	14971.5
10	15152.0
11	14825.0
12	14498.0
13	13902.0
14	13306.0
15	12596.5
16	11887.0
17	11207.5
18	10528.0
19	10086.0
20	9644.0
21	8969.5
22	8295.0
23	7802.0
24	7309.0
25	6985.0
26	7010.5
27	7360.0
28	7001.0
29	6642.0
30	8167.0
31	9692.0
32	10244.0
33	10796.0
34	12271.5
35	13747.0
36	15266.5
37	16786.0
38	19125.0
39	21464.0
40	25920.0
41	30376.0
42	37877.5
43	45379.0
44	55156.0
45	64933.0
46	143421.5
47	221910.0
48	165036.0
49	108162.0
50	97283.5
51	86405.0
52	68689.5
53	50974.0
54	42809.5
55	34645.0
56	29432.0
57	24219.0
58	22664.0
59	21109.0
60	19211.5
61	17314.0
62	14431.5
63	11549.0
64	9788.0
65	8027.0
66	6261.0
67	4495.0
68	3617.5
69	2740.0
70	2213.0
71	1686.0
72	1350.0
73	1014.0
74	764.0
75	431.5
76	349.0
77	289.0
78	229.0
79	167.0
80	105.0
81	81.5
82	58.0
83	42.5
84	27.0
85	15.5
86	4.0
87	2.0
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	973461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.01899115582245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.06834618209687	27.098139967596317
2	8.294951280520689	5.477818459389814
3	2.9233162765014993	2.895748628384245
4	1.500431517583561	1.9817094003603546
5	0.8705936741744974	1.437306241394135
6	0.554410699548016	1.0983649191041567
7	0.4043023723162714	0.93447595190523
8	0.28938404599058515	0.7644135404159399
9	0.2260924451473986	0.6718810002048203
>10	2.053744074273727	15.845573453326454
>50	0.647040251294728	14.90917329730315
>100	0.15882760881937483	7.4175306320220455
>500	0.0019021270517289959	0.37369758197482755
>1k	0.0034872329281698256	2.1288200909305512
>5k	0.0019021270517289959	4.569787573863605
>10k+	0.0012680847011526638	12.395559261824413
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGC	41617	4.2751584295621505	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCG	33844	3.4766672727515533	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTC	32273	3.3152843308566036	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	10683	1.0974245501360609	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCT	8990	0.9235090054968818	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8525	0.8757412983160086	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTC	7744	0.795512095502542	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCT	6930	0.7118929263730134	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	6153	0.6320746285675544	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTA	5314	0.5458873031379788	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTCAGTT	4780	0.49103148456897605	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGCT	2826	0.29030438815730675	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGT	2102	0.2159305817079472	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTAT	1657	0.17021739956711157	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	1587	0.16302656192698015	Illumina Single End Adapter 2 (95% over 21bp)
TGCAAGGGGATCTCAGCAGTTCTAAAAAACCAAATTTGATTGGCTCTAAAC	1476	0.1516239479547717	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTT	1284	0.13190050757041114	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCCGTTCGTATGC	1220	0.12532602744229096	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATG	1216	0.12491512243428345	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGCTTCAGTT	1119	0.1149506759901013	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	1070	0.10991708964200929	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0272625200187784E-4	0.0	0.0	0.33160034146206163	0.0
2	1.0272625200187784E-4	0.0	0.0	1.996279255152492	0.0
3	1.0272625200187784E-4	0.0	0.0	2.308977966246208	0.0
4	1.0272625200187784E-4	0.0	0.0	3.715197629899914	0.0
5	1.0272625200187784E-4	0.0	0.0	8.328839059808251	0.0
6	1.0272625200187784E-4	0.0	0.0	9.186397811519928	0.0
7	1.0272625200187784E-4	0.0	0.0	10.522866350064358	0.0
8	1.0272625200187784E-4	0.0	0.0	12.886494682375565	0.0
9	1.0272625200187784E-4	0.0	0.0	13.523911076047217	0.0
10	1.0272625200187784E-4	0.0	0.0	18.35944121027961	0.0
11	1.0272625200187784E-4	0.0	0.0	19.661188275647408	0.0
12	1.0272625200187784E-4	0.0	0.0	25.683001167997485	0.0
13	1.0272625200187784E-4	0.0	0.0	26.921674314636128	0.0
14	1.0272625200187784E-4	0.0	0.0	27.34850189170393	0.0
15	1.0272625200187784E-4	0.0	0.0	28.293583410121208	0.0
16	1.0272625200187784E-4	0.0	0.0	29.181754584929443	0.0
17	1.0272625200187784E-4	0.0	0.0	29.890257544986394	0.0
18	4.1090500800751136E-4	0.0	0.0	31.2252879160028	0.0
19	4.1090500800751136E-4	0.0	0.0	32.225841610501085	0.0
20	4.1090500800751136E-4	0.0	0.0	32.85678625029662	0.0
21	4.1090500800751136E-4	0.0	0.0	33.26748580580013	0.0
22	4.1090500800751136E-4	0.0	0.0	33.71526953827632	0.0
23	4.1090500800751136E-4	0.0	0.0	34.104910212119435	0.0
24	4.1090500800751136E-4	0.0	0.0	34.43620237482549	0.0
25	4.1090500800751136E-4	0.0	0.0	34.70534515507041	0.0
26	4.1090500800751136E-4	0.0	0.0	34.951477254866916	0.0
27	4.1090500800751136E-4	0.0	0.0	35.234898984140095	0.0
28	4.1090500800751136E-4	0.0	0.0	35.484318324000654	0.0
29	4.1090500800751136E-4	0.0	0.0	35.72942316127713	0.0
30	7.190837640131449E-4	0.0	0.0	36.03000017463463	0.0
31	8.218100160150227E-4	0.0	0.0	36.28661035213532	0.0
32	8.218100160150227E-4	0.0	0.0	36.53109883189979	0.0
33	8.218100160150227E-4	0.0	0.0	36.7726082503562	0.0
34	8.218100160150227E-4	0.0	0.0	37.02798571283287	0.0
35	8.218100160150227E-4	0.0	0.0	37.2976421243378	0.0
36	8.218100160150227E-4	0.0	0.0	37.533501598934116	0.0
37	8.218100160150227E-4	0.0	0.0	37.76987470479043	0.0
38	8.218100160150227E-4	0.0	0.0	37.987243454026405	0.0
39	8.218100160150227E-4	0.0	0.0	38.23789550891099	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGT	45	3.8380676E-10	45.000004	35
TCATACG	95	0.0	45.000004	31
TGATCGC	20	7.032755E-4	45.0	15
TCCGGAA	25	3.8903636E-5	45.0	26
CTATGTA	20	7.032755E-4	45.0	18
CTTCGAA	25	3.8903636E-5	45.0	23
CTATGCG	25	3.8903636E-5	45.0	1
CATGCGA	20	7.032755E-4	45.0	10
ACGTTAG	25	3.8903636E-5	45.0	1
CCCTAGA	20	7.032755E-4	45.0	23
GGCGTAA	40	6.8139343E-9	45.0	8
ACTCTCA	100	0.0	45.0	27
GTCTCGC	20	7.032755E-4	45.0	31
GCACGTC	20	7.032755E-4	45.0	9
CCTACGT	20	7.032755E-4	45.0	29
ATTCGTG	20	7.032755E-4	45.0	1
AAGGTCG	20	7.032755E-4	45.0	40
GCGTTAG	70	0.0	45.0	1
CGTTCCG	20	7.032755E-4	45.0	20
TACATGA	20	7.032755E-4	45.0	26
>>END_MODULE