SRR3548229_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548229_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	881344
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGC	28311	3.21225310434972	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCG	23900	2.711767482390531	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTC	22397	2.541232481301285	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10953	1.2427610558419868	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	10259	1.1640176820855421	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTCAGTT	6640	0.7533948151913441	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCT	6541	0.7421619708082201	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTC	6524	0.740233098540411	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCT	6082	0.6900824195773728	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGCT	5028	0.5704923389732046	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTA	4833	0.5483670394306877	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3322	0.3769243337448261	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGC	2977	0.33777957301575773	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	2944	0.3340352915547164	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	2323	0.2635747222423934	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCCCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCG	1774	0.20128349429961515	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGT	1745	0.19799306513688186	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	1664	0.18880255609614408	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTT	1580	0.17927165783167526	No Hit
TGCAAGGGGATCTCAGCAGTTCTAAAAAACCAAATTTGATTGGCTCTAAAC	1378	0.15635211676711933	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTAT	1244	0.1411480647738	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATG	1217	0.13808456176022077	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCCGTTCGTATGC	1158	0.13139024036017719	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1080	0.12254012054317043	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCC	996	0.11300922227870161	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATA	20	7.032389E-4	45.000004	37
CTAGACG	30	2.164923E-6	45.000004	1
CGGGTAT	40	6.8121153E-9	45.000004	6
CTGTAGC	20	7.032389E-4	45.000004	2
ACTTGCG	20	7.032389E-4	45.000004	1
GAACTTA	20	7.032389E-4	45.000004	41
CGTGAGT	20	7.032389E-4	45.000004	32
TTAGACG	20	7.032389E-4	45.000004	1
GAGTACA	20	7.032389E-4	45.000004	43
GCTATCC	30	2.164923E-6	45.000004	8
TTCACGG	30	2.164923E-6	45.000004	2
CGATTCG	20	7.032389E-4	45.000004	10
TCGATAG	30	2.164923E-6	45.000004	1
ATCGTAG	20	7.032389E-4	45.000004	1
ATACTAT	60	0.0	45.000004	45
ACGGCTT	20	7.032389E-4	45.000004	22
GAGCGTT	20	7.032389E-4	45.000004	9
CGTACTC	30	2.164923E-6	45.000004	10
TCGTACA	20	7.032389E-4	45.000004	17
GTTCACG	20	7.032389E-4	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content