##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548229_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	881344
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.398545857236222	30.0	25.0	31.0	16.0	33.0
2	27.791377714036745	30.0	25.0	31.0	16.0	33.0
3	27.72699082310653	30.0	25.0	31.0	16.0	34.0
4	32.09916105402658	35.0	32.0	35.0	25.0	37.0
5	33.157829405998115	35.0	32.0	35.0	28.0	37.0
6	31.915411008641346	35.0	32.0	35.0	25.0	37.0
7	33.61815023418779	35.0	33.0	35.0	30.0	37.0
8	32.943718911117564	35.0	33.0	36.0	27.0	37.0
9	35.56118269370416	37.0	34.0	39.0	30.0	39.0
10	35.22208467976182	37.0	34.0	39.0	30.0	39.0
11	35.725377378186046	37.0	35.0	39.0	31.0	39.0
12	35.73693359233171	37.0	35.0	39.0	31.0	39.0
13	35.655368391910535	37.0	35.0	39.0	31.0	39.0
14	36.327106101590296	38.0	35.0	40.0	30.0	41.0
15	36.60619122612737	38.0	35.0	40.0	31.0	41.0
16	36.31054730048653	38.0	34.0	40.0	31.0	41.0
17	35.761624291990415	37.0	34.0	39.0	30.0	41.0
18	35.90047359487328	37.0	34.0	39.0	30.0	40.0
19	35.48498996986421	37.0	34.0	39.0	29.0	40.0
20	35.30061701219955	37.0	34.0	39.0	29.0	40.0
21	35.75715157759059	37.0	34.0	39.0	30.0	40.0
22	36.26807466778012	38.0	35.0	40.0	31.0	40.0
23	36.3320145141965	38.0	35.0	40.0	31.0	41.0
24	36.34847687168688	38.0	35.0	40.0	31.0	41.0
25	35.615517890857596	37.0	34.0	40.0	29.0	41.0
26	35.88367311742066	38.0	34.0	40.0	30.0	41.0
27	35.96461880945465	38.0	35.0	40.0	30.0	41.0
28	35.81650184264033	38.0	35.0	40.0	30.0	41.0
29	35.964946717740176	38.0	35.0	40.0	30.0	41.0
30	35.429840107835304	38.0	34.0	40.0	27.0	41.0
31	35.41341292389804	38.0	34.0	40.0	28.0	41.0
32	35.18418914748384	38.0	34.0	40.0	27.0	41.0
33	34.99698869000073	38.0	34.0	40.0	25.0	41.0
34	34.701463900588195	38.0	34.0	40.0	23.0	41.0
35	34.69449272928618	38.0	34.0	40.0	23.0	41.0
36	34.40476703761528	38.0	34.0	40.0	21.0	41.0
37	34.506620570401566	38.0	34.0	40.0	21.0	41.0
38	34.359663196209425	38.0	34.0	40.0	21.0	41.0
39	34.16238267918089	38.0	33.0	40.0	20.0	41.0
40	34.18161467032169	38.0	33.0	40.0	21.0	41.0
41	33.393659002614186	37.0	33.0	40.0	18.0	41.0
42	33.915435970517755	37.0	33.0	40.0	19.0	41.0
43	33.838563602861086	37.0	33.0	40.0	18.0	41.0
44	34.04248284438312	37.0	34.0	40.0	19.0	41.0
45	34.128308583254665	38.0	34.0	40.0	20.0	41.0
46	33.87869095381599	37.0	33.0	40.0	20.0	41.0
47	33.72047010021059	37.0	33.0	40.0	20.0	41.0
48	33.785103206012636	37.0	33.0	40.0	20.0	41.0
49	33.94283957228959	37.0	33.0	40.0	20.0	41.0
50	33.73495025778811	37.0	33.0	40.0	20.0	41.0
51	31.760371659647085	35.0	30.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	11.0
10	18.0
11	20.0
12	20.0
13	34.0
14	44.0
15	80.0
16	195.0
17	483.0
18	1106.0
19	2240.0
20	3981.0
21	5999.0
22	7679.0
23	8903.0
24	10687.0
25	13776.0
26	16926.0
27	17698.0
28	17615.0
29	19103.0
30	23253.0
31	31689.0
32	44724.0
33	63410.0
34	83410.0
35	102030.0
36	137097.0
37	157674.0
38	97682.0
39	13756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.864420702926445	19.039557766320527	22.751048398809093	15.34497313194394
2	30.182199005155763	35.60244354077409	21.767663931450148	12.447693522619998
3	22.289934463728127	34.99745842712947	31.144933192941686	11.567673916200711
4	21.131703398446007	22.706003558202017	43.263470336213786	12.898822707138189
5	29.129148210006534	25.10586104858035	29.734246786725727	16.030743954687388
6	17.09990650642655	41.80535636482463	29.13470790066081	11.960029228088011
7	61.370021240287564	6.047468411880038	27.94935916055479	4.633151187277612
8	54.94778429307966	15.332832582964201	18.631090697843295	11.088292426112845
9	50.39462457337884	6.8195846343765885	21.98086104858035	20.80492974366422
10	35.87781842277249	21.592817333526977	26.135424987292133	16.393939256408395
11	27.146267518698714	21.673943431849537	32.221470844528355	18.958318204923387
12	21.141801612083363	14.852316462130563	38.391819766175296	25.61406215961078
13	21.359991104494952	21.23903946699586	43.99542063031007	13.405548798199113
14	16.23815445501416	31.162973821799433	31.154464091206158	21.444407631980248
15	10.785799869290539	21.13975927674098	49.73778683465253	18.336654019315954
16	10.538563829787234	24.784760547527412	35.630241994045456	29.046433628639896
17	10.7159066153511	25.660241630963622	37.27829315227652	26.345558601408758
18	13.51572144361339	22.8166300559146	38.15161843729577	25.516030063176242
19	15.122131653474694	28.43929271657832	33.34259948442379	23.0959761455232
20	17.554326120107472	24.54819003703435	40.21267518698715	17.684808655871034
21	15.94564664875463	33.77704959697916	33.849212112410136	16.428091641856074
22	12.622199731319439	22.505400842349864	35.260579297073555	29.611820129257133
23	13.638488490305715	32.094165274852955	32.860381417471494	21.406964817369836
24	17.902771222133467	22.797114407087356	31.66448061142982	27.63563375934936
25	13.789394379493139	32.73443740469102	29.594119889623123	23.882048326192724
26	15.610930578752452	23.69619581003558	34.68781769660881	26.005055914603155
27	19.75290011618619	27.28344437586232	28.33411244644543	24.629543061506062
28	13.837729649262945	24.199631471933774	40.05439419795222	21.908244680851062
29	22.389214653983007	19.923548580350012	30.765966523854477	26.921270241812508
30	17.634771439982572	25.5335033766611	31.237632524871106	25.594092658485224
31	20.742978904945176	25.0296138624646	28.24141311451601	25.985994118074213
32	24.226181831384793	25.831230484351174	28.36281860431341	21.57976907995062
33	15.627382724566116	21.420693849393654	26.915597088083654	36.036326337956574
34	23.598504102824776	23.088260656451965	31.29016592840026	22.023069312323
35	20.615559872195195	18.342554099193958	30.19592803717958	30.845957991431266
36	23.31223676566698	18.178032641057296	34.54042916273328	23.969301430542444
37	17.99422246024254	21.275007261636773	37.199322852370926	23.531447425749764
38	20.784619853314936	20.688743555297364	31.844092658485224	26.682543932902476
39	21.25390312976545	22.13074577009658	35.60516665456394	21.010184445574033
40	24.899698642073922	18.50684863118147	31.050191525669884	25.543261201074724
41	19.27941870597633	24.91002378186043	30.104703725219668	25.705853786943578
42	28.20238181686152	17.424183973567644	34.248602134921214	20.124832074649625
43	23.16167126570329	16.33108071309273	35.4898881707937	25.01735985041028
44	20.76884848594873	18.982485839808295	33.41498892600392	26.833676748239053
45	19.244585542081186	16.749078679834433	32.540529010238906	31.46580676784547
46	28.16607363299688	20.660604712802268	28.877373647520148	22.295948006680703
47	15.940767736547818	18.822389441580132	41.41311451601191	23.82372830586014
48	20.155013252487112	20.550205141238838	30.410600174279285	28.884181431994772
49	20.410872485658267	14.405158485222568	40.372431195991574	24.811537833127588
50	21.356814138406797	14.054330658630453	36.90534002614189	27.683515176820855
51	18.4139223731029	15.735286108488852	28.98902131290393	36.86177020550432
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1309.0
1	1283.5
2	1258.0
3	9162.0
4	17066.0
5	11651.0
6	6236.0
7	6229.5
8	6223.0
9	6390.5
10	6558.0
11	6659.0
12	6760.0
13	6625.0
14	6490.0
15	6313.0
16	6136.0
17	5948.0
18	5760.0
19	5862.5
20	5965.0
21	5537.0
22	5109.0
23	4895.5
24	4682.0
25	4841.5
26	5912.5
27	6824.0
28	6886.0
29	6948.0
30	8071.5
31	9195.0
32	9441.0
33	9687.0
34	10256.0
35	10825.0
36	11937.5
37	13050.0
38	14783.5
39	16517.0
40	19765.0
41	23013.0
42	29989.5
43	36966.0
44	48393.5
45	59821.0
46	125525.0
47	191229.0
48	164053.0
49	136877.0
50	121768.5
51	106660.0
52	83172.5
53	59685.0
54	47774.0
55	35863.0
56	29127.0
57	22391.0
58	19614.0
59	16837.0
60	14878.5
61	12920.0
62	10745.0
63	8570.0
64	7076.0
65	5582.0
66	4318.5
67	3055.0
68	2438.5
69	1822.0
70	1448.5
71	1075.0
72	850.5
73	626.0
74	450.5
75	257.0
76	239.0
77	186.5
78	134.0
79	94.0
80	54.0
81	44.5
82	35.0
83	22.5
84	10.0
85	5.5
86	1.0
87	0.5
88	0.0
89	1.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	881344.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.286477963761175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.39276694472903	21.787021297900274
2	10.347962412744396	6.061107463413353
3	4.404051876569108	3.869375047032068
4	2.277370280785664	2.667846181742158
5	1.5011253104485038	2.198133666264713
6	1.0243179427098297	1.7999198914233967
7	0.7380823315454954	1.5131082356774037
8	0.5528093778521439	1.295187173010187
9	0.41076652252713347	1.0826914239217533
>10	3.7670723051595862	24.197914044594455
>50	0.4608990371020171	8.81168074735207
>100	0.10826224140668265	5.729401785328077
>500	0.005099308472053893	1.0801933870842506
>1k	0.005491562969904194	3.358904014139786
>5k	0.0019612724892514974	3.539950996809655
>10k+	0.0019612724892514974	11.007564644306381
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGC	28311	3.21225310434972	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCG	23900	2.711767482390531	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTC	22397	2.541232481301285	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10953	1.2427610558419868	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	10259	1.1640176820855421	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTCAGTT	6640	0.7533948151913441	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCT	6541	0.7421619708082201	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTC	6524	0.740233098540411	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCT	6082	0.6900824195773728	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGCT	5028	0.5704923389732046	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTA	4833	0.5483670394306877	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3322	0.3769243337448261	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGC	2977	0.33777957301575773	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	2944	0.3340352915547164	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	2323	0.2635747222423934	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCCCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCG	1774	0.20128349429961515	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGT	1745	0.19799306513688186	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC	1664	0.18880255609614408	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTT	1580	0.17927165783167526	No Hit
TGCAAGGGGATCTCAGCAGTTCTAAAAAACCAAATTTGATTGGCTCTAAAC	1378	0.15635211676711933	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTAT	1244	0.1411480647738	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATG	1217	0.13808456176022077	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCCGTTCGTATGC	1158	0.13139024036017719	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1080	0.12254012054317043	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCC	996	0.11300922227870161	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1346307457700966E-4	0.0	0.0	0.6212103333091279	0.0
2	1.1346307457700966E-4	0.0	0.0	2.738204378766974	0.0
3	1.1346307457700966E-4	0.0	0.0	3.478210551158231	0.0
4	1.1346307457700966E-4	0.0	0.0	5.337416491177112	0.0
5	1.1346307457700966E-4	0.0	0.0	10.529827173044804	0.0
6	1.1346307457700966E-4	0.0	0.0	12.585324232081911	0.0
7	1.1346307457700966E-4	0.0	0.0	14.674746750417544	0.0
8	1.1346307457700966E-4	0.0	0.0	18.921442705685863	0.0
9	1.1346307457700966E-4	0.0	0.0	20.216283312758694	0.0
10	1.1346307457700966E-4	0.0	0.0	25.779491322344057	0.0
11	1.1346307457700966E-4	0.0	0.0	28.829492230048654	0.0
12	1.1346307457700966E-4	0.0	0.0	35.31356655290102	0.0
13	1.1346307457700966E-4	0.0	0.0	36.72050867765594	0.0
14	1.1346307457700966E-4	0.0	0.0	37.21543460896086	0.0
15	1.1346307457700966E-4	0.0	0.0	38.42597215162298	0.0
16	1.1346307457700966E-4	0.0	0.0	39.86718012490015	0.0
17	1.1346307457700966E-4	0.0	0.0	41.22442542299034	0.0
18	3.4038922373102895E-4	0.0	0.0	43.53181050758841	0.0
19	3.4038922373102895E-4	0.0	0.0	45.36707573887154	0.0
20	3.4038922373102895E-4	0.0	0.0	46.48026196354658	0.0
21	3.4038922373102895E-4	0.0	0.0	47.27824776704669	0.0
22	3.4038922373102895E-4	0.0	0.0	48.076233570546805	0.0
23	3.4038922373102895E-4	0.0	0.0	48.8258841042771	0.0
24	3.4038922373102895E-4	0.0	0.0	49.41600555515213	0.0
25	3.4038922373102895E-4	0.0	0.0	49.90332946046039	0.0
26	3.4038922373102895E-4	0.0	0.0	50.33006408394452	0.0
27	3.4038922373102895E-4	0.0	0.0	50.77937785926948	0.0
28	3.4038922373102895E-4	0.0	0.0	51.1760447679907	0.0
29	3.4038922373102895E-4	0.0	0.0	51.589617674823906	0.0
30	5.673153728850482E-4	0.0	0.0	52.03552755791155	0.0
31	5.673153728850482E-4	0.0	0.0	52.46543914748384	0.0
32	5.673153728850482E-4	0.0	0.0	52.85053282259821	0.0
33	5.673153728850482E-4	0.0	0.0	53.234038014668506	0.0
34	5.673153728850482E-4	0.0	0.0	53.5953044441217	0.0
35	5.673153728850482E-4	0.0	0.0	53.99355983588701	0.0
36	5.673153728850482E-4	0.0	0.0	54.349606963909665	0.0
37	5.673153728850482E-4	0.0	0.0	54.68228069856946	0.0
38	5.673153728850482E-4	0.0	0.0	55.00224656887662	0.0
39	5.673153728850482E-4	0.0	0.0	55.353187858543315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATA	20	7.032389E-4	45.000004	37
CTAGACG	30	2.164923E-6	45.000004	1
CGGGTAT	40	6.8121153E-9	45.000004	6
CTGTAGC	20	7.032389E-4	45.000004	2
ACTTGCG	20	7.032389E-4	45.000004	1
GAACTTA	20	7.032389E-4	45.000004	41
CGTGAGT	20	7.032389E-4	45.000004	32
TTAGACG	20	7.032389E-4	45.000004	1
GAGTACA	20	7.032389E-4	45.000004	43
GCTATCC	30	2.164923E-6	45.000004	8
TTCACGG	30	2.164923E-6	45.000004	2
CGATTCG	20	7.032389E-4	45.000004	10
TCGATAG	30	2.164923E-6	45.000004	1
ATCGTAG	20	7.032389E-4	45.000004	1
ATACTAT	60	0.0	45.000004	45
ACGGCTT	20	7.032389E-4	45.000004	22
GAGCGTT	20	7.032389E-4	45.000004	9
CGTACTC	30	2.164923E-6	45.000004	10
TCGTACA	20	7.032389E-4	45.000004	17
GTTCACG	20	7.032389E-4	45.000004	1
>>END_MODULE