FastQCFastQC Report
Sat 18 Jun 2016
SRR3548228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982202
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC194351.9787172088837124No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG189711.9314764172746544No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC158801.616775367999658No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102911.0477478156224482No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC53920.5489705783535362Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT46170.4700662389203036No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC40270.40999712890016515No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT37100.3777227087707009No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA28920.29444045114955986No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT26240.2671548215132936No Hit
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT15670.15953948373145238Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC14690.1495619027450565Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT10810.11005882700299938No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGT10660.10853164623977553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAC650.045.00000413
GCGAGAC750.045.021
GTGCATC207.0327864E-445.025
ACTATCG207.0327864E-445.026
TCGCATC453.8380676E-1045.022
GGTACGT207.0327864E-445.037
ACACGAT207.0327864E-445.023
CGAACAG207.0327864E-445.015
GGTAAGT406.8139343E-945.08
TACTTCG207.0327864E-445.029
AGTCAAT253.8903905E-545.032
CAACGTA351.2116834E-745.038
TACCAGG406.8139343E-945.02
CGAAACG207.0327864E-445.034
ACGTTCA253.8903905E-545.020
CTATCCC253.8903905E-545.038
ACGCAAT253.8903905E-545.016
TAGGTGA351.2116834E-745.032
CGGCATA207.0327864E-445.06
CTATACG453.8380676E-1045.01