##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548228_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	982202
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.714839717288296	28.0	25.0	31.0	16.0	33.0
2	27.63032553385149	30.0	25.0	31.0	16.0	33.0
3	27.463617463617464	30.0	25.0	31.0	16.0	33.0
4	30.197214015039677	33.0	28.0	35.0	19.0	37.0
5	31.822689222787165	33.0	32.0	35.0	26.0	37.0
6	31.215142099079415	35.0	30.0	35.0	17.0	37.0
7	32.04906424544035	35.0	31.0	35.0	26.0	35.0
8	33.200129912176926	35.0	33.0	35.0	28.0	37.0
9	34.43645502656276	37.0	33.0	39.0	28.0	39.0
10	34.06682943019867	37.0	32.0	39.0	27.0	39.0
11	34.25516136191944	37.0	32.0	39.0	27.0	39.0
12	33.67287991675847	35.0	32.0	38.0	25.0	39.0
13	33.84343139191327	35.0	32.0	39.0	25.0	39.0
14	34.55168183326851	37.0	33.0	39.0	25.0	40.0
15	35.5465179260478	37.0	33.0	40.0	27.0	41.0
16	35.58261844304939	37.0	34.0	40.0	29.0	41.0
17	35.08202182443123	37.0	33.0	39.0	27.0	41.0
18	34.89873264359063	37.0	33.0	39.0	27.0	40.0
19	34.177856489805556	36.0	32.0	39.0	26.0	40.0
20	34.086381416449974	35.0	32.0	38.0	27.0	40.0
21	34.467869134862276	35.0	33.0	39.0	27.0	40.0
22	34.69977356999884	36.0	33.0	39.0	27.0	40.0
23	35.107029918489275	36.0	33.0	39.0	29.0	40.0
24	34.74754480239299	35.0	33.0	39.0	27.0	40.0
25	33.98413259187011	35.0	33.0	39.0	24.0	40.0
26	33.8791664036522	35.0	32.0	39.0	24.0	40.0
27	34.407480335002376	35.0	33.0	39.0	25.0	40.0
28	34.282817587420915	35.0	33.0	39.0	25.0	40.0
29	34.38321445079525	36.0	33.0	39.0	25.0	40.0
30	33.834622613270994	35.0	33.0	39.0	23.0	40.0
31	33.73277798253312	35.0	33.0	39.0	23.0	40.0
32	33.16832484560202	35.0	32.0	39.0	20.0	40.0
33	32.947354006609636	36.0	32.0	39.0	17.0	40.0
34	32.53113921576213	36.0	32.0	39.0	15.0	40.0
35	32.42725325340409	36.0	32.0	40.0	10.0	40.0
36	32.04175414018705	36.0	31.0	40.0	10.0	40.0
37	31.962640067929	35.0	31.0	40.0	10.0	40.0
38	31.929677398335578	35.0	31.0	39.0	10.0	40.0
39	31.69743189282856	35.0	30.0	39.0	10.0	40.0
40	31.56741077700921	35.0	30.0	39.0	10.0	40.0
41	31.25371766703794	35.0	29.0	39.0	9.0	40.0
42	31.332016224768427	35.0	30.0	39.0	9.0	40.0
43	31.33209869252964	35.0	30.0	39.0	9.0	40.0
44	31.247319797760543	35.0	30.0	39.0	8.0	40.0
45	31.348975058083774	35.0	30.0	39.0	9.0	40.0
46	31.1350964465558	35.0	29.0	39.0	8.0	40.0
47	31.208908147203935	35.0	30.0	39.0	8.0	40.0
48	31.034654785879077	35.0	29.0	39.0	8.0	40.0
49	31.129529363613596	35.0	29.0	39.0	8.0	40.0
50	31.035989541866133	35.0	29.0	39.0	8.0	40.0
51	28.950248523216203	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	21.0
9	54.0
10	82.0
11	65.0
12	51.0
13	66.0
14	112.0
15	309.0
16	734.0
17	1591.0
18	3224.0
19	5354.0
20	8156.0
21	11596.0
22	15530.0
23	20228.0
24	25628.0
25	31455.0
26	33326.0
27	32778.0
28	31320.0
29	34391.0
30	40719.0
31	51941.0
32	66899.0
33	83368.0
34	94356.0
35	94752.0
36	109190.0
37	110211.0
38	67480.0
39	7213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.16181192870713	18.876361481650413	21.958110449785277	25.00371613985718
2	37.722790220341636	27.802733042693866	23.136992186943214	11.337484550021278
3	23.88816149834759	27.52834956556798	37.09277724948636	11.490711686598072
4	21.49160763264583	22.136688787031588	42.86389154165844	13.507812038664143
5	24.299482183909216	25.790926917273637	36.05745050407146	13.852140394745685
6	17.39733781849355	36.769625799988184	35.99575240123722	9.837283980281041
7	65.9989492996349	4.461913129885706	27.073453322229028	2.46568424825036
8	63.65034890989837	10.27690841598775	21.59606679685034	4.476675877263537
9	59.36548693649575	5.510780878067852	23.087715154316527	12.03601703111987
10	36.45288850969556	22.13546704242101	28.68513808768461	12.726506360198819
11	31.793867249303098	20.742983622513496	33.865742484743464	13.597406643439944
12	27.628328999533704	17.210818141278477	38.02089590532294	17.13995695386489
13	19.099024436928453	27.129551762264786	40.858906823647274	12.912516977159486
14	15.080299164530311	33.35036988318085	35.43680424189729	16.13252671039155
15	11.926976324625688	21.852531353021067	52.64151366012286	13.578978662230377
16	12.572464727214971	21.844182764848778	45.237741320013605	20.345611187922646
17	13.328317392959901	20.367908027065713	40.16383595227865	26.13993862769573
18	15.807848080130157	22.278716598011407	42.7933357903975	19.12009953146094
19	18.721912600463043	23.861486741016613	37.72594639391897	19.690654264601378
20	21.253571057684674	21.995068224255295	40.549092752814595	16.202267965245436
21	17.952213495798215	26.104711658090697	39.70934695714324	16.23372788896785
22	16.856410392159656	21.30844775310985	38.38232868595258	23.452813168777908
23	15.814262239335697	27.946084410335143	36.50745976896809	19.732193581361066
24	17.651969757748407	21.789204257372717	39.58992142145913	20.968904563419745
25	14.44132673319745	27.355370891120156	38.68684853013942	19.516453845542976
26	14.269569803360206	25.39732152856541	39.488312994679305	20.844795673395087
27	17.51014557087035	27.514604938698966	36.2552713189344	18.71997817149629
28	13.741368883386512	25.50839847607722	44.38801794335585	16.362214697180416
29	15.45079321768842	21.692890057238735	42.810236590843836	20.04608013422901
30	15.95720635877345	25.117643824793678	40.82866864453544	18.09648117189743
31	22.84153361528484	24.865048126556452	33.49260131826243	18.800816939896272
32	22.73595451852063	25.109702484824915	36.65020026430408	15.50414273235037
33	19.860782201624513	24.573865661035104	32.498813889607234	23.066538247733153
34	20.106047432198267	26.33409420872692	35.12210319262229	18.437755166452522
35	19.157566366185367	22.79673631289694	35.77431119056976	22.271386130347935
36	27.506867222831964	21.600241090936485	33.21068374937131	17.682207936860237
37	19.56237108049057	25.066126927047595	39.432010930541786	15.939491061920052
38	23.057782411357337	24.889483018768033	29.29081797837919	22.761916591495435
39	22.59433395574434	25.907399903482176	33.426423485189396	18.071842655584085
40	22.3948841480673	23.076108580516024	33.6260769169682	20.902930354448472
41	20.80132192766865	26.23869631705087	30.358622768025313	22.601358987255168
42	24.561953651081957	22.6686567528879	33.21882871344184	19.550560882588304
43	24.805691700892485	23.224041490446975	31.11427180966848	20.85599499899206
44	21.23555032467863	25.768324641977923	31.753549677153988	21.242575356189462
45	18.50362756337291	26.10827507987155	31.52661061573892	23.861486741016613
46	24.421045772661834	26.87614156762051	30.540764527052477	18.162048132665177
47	18.901610870269046	24.28634842934549	36.35891598673185	20.453124713653605
48	21.918912810195863	24.334200093259838	31.09502933205186	22.651857764492437
49	20.02378329508594	21.333289893524956	36.358305114426564	22.28462169696254
50	20.625085267592613	23.050961003948274	33.91756481864219	22.406388909816922
51	18.859562493254952	23.82534346295365	29.85149694258411	27.463597101207288
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	465.0
2	812.0
3	12778.0
4	24744.0
5	20403.0
6	16062.0
7	16387.5
8	16713.0
9	17191.0
10	17669.0
11	17341.5
12	17014.0
13	16307.5
14	15601.0
15	14748.5
16	13896.0
17	13100.5
18	12305.0
19	11447.0
20	10589.0
21	10318.0
22	10047.0
23	10120.0
24	10193.0
25	10149.5
26	10860.0
27	11614.0
28	11726.0
29	11838.0
30	13388.0
31	14938.0
32	15953.0
33	16968.0
34	20392.5
35	23817.0
36	23834.5
37	23852.0
38	25132.0
39	26412.0
40	33642.5
41	40873.0
42	51205.0
43	61537.0
44	66128.5
45	70720.0
46	108998.5
47	147277.0
48	115889.0
49	84501.0
50	78867.5
51	73234.0
52	62466.0
53	51698.0
54	44029.0
55	36360.0
56	31957.0
57	27554.0
58	26134.0
59	24714.0
60	21982.0
61	19250.0
62	17033.5
63	14817.0
64	12230.5
65	9644.0
66	7915.5
67	6187.0
68	4828.0
69	3469.0
70	2691.5
71	1914.0
72	1760.0
73	1606.0
74	1176.5
75	607.0
76	467.0
77	348.0
78	229.0
79	141.5
80	54.0
81	36.5
82	19.0
83	16.0
84	13.0
85	9.0
86	5.0
87	3.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	982202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.404715425953704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.94831120095051	26.879164195302497
2	8.1838553118273	5.303910049338866
3	2.772750332892582	2.695505564538076
4	1.373906928781798	1.7808425219568083
5	0.7434076646222056	1.2044956908777704
6	0.4895308049472176	0.9517863855931582
7	0.35453741781742865	0.8042078892558356
8	0.23105551230187754	0.598983049899223
9	0.18246071340141046	0.5321328744770282
>10	1.5767575903744269	11.909877650823544
>50	0.673701508091163	15.900009087640651
>100	0.46168177745109706	20.997686553253374
>500	0.003539024078022325	0.7433418325407182
>1k	0.0028955651547455394	2.403402421537332
>5k	3.2172946163839324E-4	0.5621457448891378
>10k+	0.001286917846553573	6.732508488076011
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	19435	1.9787172088837124	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	18971	1.9314764172746544	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	15880	1.616775367999658	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10291	1.0477478156224482	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	5392	0.5489705783535362	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	4617	0.4700662389203036	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC	4027	0.40999712890016515	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	3710	0.3777227087707009	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA	2892	0.29444045114955986	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT	2624	0.2671548215132936	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	1567	0.15953948373145238	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1469	0.1495619027450565	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT	1081	0.11005882700299938	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGT	1066	0.10853164623977553	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1800037059586521	0.0
2	0.0	0.0	0.0	1.1076133015408236	0.0
3	0.0	0.0	0.0	1.339235717296442	0.0
4	0.0	0.0	0.0	2.140191121581915	0.0
5	0.0	0.0	0.0	4.683456152604047	0.0
6	0.0	0.0	0.0	5.356535620982242	0.0
7	0.0	0.0	0.0	6.176428066731691	0.0
8	0.0	0.0	0.0	7.635292943814002	0.0
9	0.0	0.0	0.0	8.092632676374107	0.0
10	0.0	0.0	0.0	10.980022439376015	0.0
11	0.0	0.0	0.0	11.9213766618272	0.0
12	0.0	0.0	0.0	14.99060274770363	0.0
13	0.0	0.0	0.0	15.751036955738229	0.0
14	0.0	0.0	0.0	16.030001975153787	0.0
15	0.0	0.0	0.0	16.589459194748127	0.0
16	0.0	0.0	0.0	17.202978613360592	0.0
17	0.0	0.0	0.0	17.707660949580635	0.0
18	2.036241017631811E-4	0.0	0.0	18.678744290889245	0.0
19	2.036241017631811E-4	0.0	0.0	19.307739141235714	0.0
20	2.036241017631811E-4	0.0	0.0	19.718245330390285	0.0
21	2.036241017631811E-4	0.0	0.0	20.038036982209363	0.0
22	2.036241017631811E-4	0.0	0.0	20.362206552216346	0.0
23	2.036241017631811E-4	0.0	0.0	20.629972246034928	0.0
24	2.036241017631811E-4	0.0	0.0	20.865056271520523	0.0
25	2.036241017631811E-4	0.0	0.0	21.049539707717965	0.0
26	2.036241017631811E-4	0.0	0.0	21.23147784264337	0.0
27	2.036241017631811E-4	0.0	0.0	21.4359164408136	0.0
28	2.036241017631811E-4	0.0	0.0	21.61256034909316	0.0
29	2.036241017631811E-4	0.0	0.0	21.81852612802662	0.0
30	2.036241017631811E-4	0.0	0.0	22.060940621175686	0.0
31	2.036241017631811E-4	0.0	0.0	22.269655325482944	0.0
32	2.036241017631811E-4	0.0	0.0	22.460451108835045	0.0
33	2.036241017631811E-4	0.0	0.0	22.642796491963974	0.0
34	2.036241017631811E-4	0.0	0.0	22.8660703195473	0.0
35	2.036241017631811E-4	0.0	0.0	23.09443474967471	0.0
36	2.036241017631811E-4	0.0	0.0	23.288081270451496	0.0
37	2.036241017631811E-4	0.0	0.0	23.484680340703846	0.0
38	2.036241017631811E-4	0.0	0.0	23.66661847562925	0.0
39	2.036241017631811E-4	0.0	0.0	23.856497950523416	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGAC	65	0.0	45.000004	13
GCGAGAC	75	0.0	45.0	21
GTGCATC	20	7.0327864E-4	45.0	25
ACTATCG	20	7.0327864E-4	45.0	26
TCGCATC	45	3.8380676E-10	45.0	22
GGTACGT	20	7.0327864E-4	45.0	37
ACACGAT	20	7.0327864E-4	45.0	23
CGAACAG	20	7.0327864E-4	45.0	15
GGTAAGT	40	6.8139343E-9	45.0	8
TACTTCG	20	7.0327864E-4	45.0	29
AGTCAAT	25	3.8903905E-5	45.0	32
CAACGTA	35	1.2116834E-7	45.0	38
TACCAGG	40	6.8139343E-9	45.0	2
CGAAACG	20	7.0327864E-4	45.0	34
ACGTTCA	25	3.8903905E-5	45.0	20
CTATCCC	25	3.8903905E-5	45.0	38
ACGCAAT	25	3.8903905E-5	45.0	16
TAGGTGA	35	1.2116834E-7	45.0	32
CGGCATA	20	7.0327864E-4	45.0	6
CTATACG	45	3.8380676E-10	45.0	1
>>END_MODULE