FastQCFastQC Report
Sat 18 Jun 2016
SRR3548226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564210
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGC115292.043388100175467No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG111001.967352581485617No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTC75131.3315963914145441No Hit
GCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC47860.8482657166657805No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31690.5616703000655784No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT25640.45444072242604705No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT25490.45178213785647187No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTC24470.4337037627833608No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGTTCTT21480.38070931036316263No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT20340.3605040676343914No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTA16140.28606369968628703No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC13630.2415767178887294No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGGTCTTCGTATGCCGTCTTC8050.1426773719005335No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGT7540.13363818436397795No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTAT6350.11254674677868169No Hit
ACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC6140.10882472838127648No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6130.10864748940997146No Hit
TCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC5700.10102621364385601No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATA302.1635296E-645.00000416
CCCTAAT302.1635296E-645.00000440
CGGTTGA302.1635296E-645.00000430
GCCGATC302.1635296E-645.0000049
CGCTATT302.1635296E-645.00000430
AGCGAAG302.1635296E-645.0000041
TGAGGGT750.045.0000044
GACGCAC302.1635296E-645.0000049
TCCCCGT302.1635296E-645.00000424
GTAAGGT302.1635296E-645.00000424
ATCGGCC302.1635296E-645.00000415
AGGTGTC302.1635296E-645.00000422
AATCGAG302.1635296E-645.0000041
TGATCCA207.0302305E-445.028
AAGTATC207.0302305E-445.021
GTGCAGT207.0302305E-445.03
CTATGGT207.0302305E-445.028
AGACTGC351.2104647E-745.037
GTCGCGG207.0302305E-445.036
CGAACGT207.0302305E-445.017