##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	564210
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.639471118909626	28.0	25.0	31.0	16.0	33.0
2	27.21930486875454	30.0	25.0	31.0	16.0	33.0
3	27.17519895074529	30.0	25.0	31.0	16.0	33.0
4	30.5494904379575	33.0	28.0	35.0	19.0	37.0
5	31.91391148685773	33.0	32.0	35.0	26.0	37.0
6	30.873224508604952	33.0	30.0	35.0	17.0	37.0
7	31.608534056468336	33.0	30.0	35.0	25.0	35.0
8	32.65761152762269	35.0	32.0	35.0	27.0	37.0
9	33.99661650803779	35.0	32.0	37.0	27.0	39.0
10	33.57289838889775	35.0	32.0	37.0	27.0	39.0
11	33.71659842966272	35.0	32.0	39.0	26.0	39.0
12	33.260582052781764	35.0	31.0	37.0	25.0	39.0
13	33.32700767444746	35.0	32.0	38.0	24.0	39.0
14	33.91638574289715	36.0	32.0	39.0	24.0	40.0
15	34.93031495365201	37.0	33.0	39.0	27.0	40.0
16	35.05105900285355	36.0	33.0	39.0	27.0	40.0
17	34.527472040552276	36.0	32.0	39.0	27.0	40.0
18	34.42634302830506	36.0	32.0	39.0	27.0	40.0
19	33.72502082557913	36.0	32.0	38.0	25.0	40.0
20	33.692598500558304	35.0	32.0	38.0	25.0	40.0
21	33.980532071391856	35.0	32.0	38.0	26.0	40.0
22	34.43685861647259	35.0	33.0	39.0	27.0	40.0
23	34.679931231279134	35.0	33.0	39.0	27.0	40.0
24	34.41363499406249	35.0	33.0	39.0	26.0	40.0
25	33.46997749065064	35.0	32.0	39.0	23.0	40.0
26	33.52163910600663	35.0	32.0	39.0	23.0	40.0
27	33.87940305914464	35.0	33.0	39.0	24.0	40.0
28	33.75769128515978	35.0	33.0	39.0	24.0	40.0
29	33.66461778415838	35.0	33.0	39.0	23.0	40.0
30	32.863720954963576	35.0	31.0	39.0	20.0	40.0
31	32.915586395136565	35.0	31.0	39.0	21.0	40.0
32	32.370535793410255	35.0	31.0	39.0	16.0	40.0
33	32.059437089027135	35.0	30.0	39.0	15.0	40.0
34	31.989300083302318	35.0	30.0	39.0	12.0	40.0
35	31.648669821520357	35.0	30.0	39.0	10.0	40.0
36	31.408450754151822	35.0	30.0	39.0	10.0	40.0
37	31.240328955530742	35.0	30.0	39.0	9.0	40.0
38	30.926332393966785	35.0	29.0	39.0	9.0	40.0
39	30.99435316637422	35.0	29.0	39.0	8.0	40.0
40	30.56168802396271	35.0	27.0	39.0	8.0	40.0
41	30.416603746831854	35.0	26.0	38.0	8.0	40.0
42	30.69284131794899	35.0	28.0	38.0	8.0	40.0
43	30.5304496552702	35.0	27.0	38.0	8.0	40.0
44	30.52471951932791	35.0	27.0	38.0	8.0	40.0
45	30.531493592811188	35.0	27.0	38.0	8.0	40.0
46	30.284940004608213	35.0	27.0	38.0	8.0	40.0
47	30.360631679693732	35.0	27.0	38.0	8.0	40.0
48	30.176799418656174	35.0	26.0	38.0	8.0	40.0
49	30.309026780808566	35.0	27.0	38.0	8.0	40.0
50	30.2010049449673	35.0	27.0	38.0	8.0	40.0
51	27.91940943974761	32.0	22.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	43.0
10	48.0
11	54.0
12	43.0
13	74.0
14	100.0
15	320.0
16	753.0
17	1739.0
18	3179.0
19	5132.0
20	7242.0
21	9594.0
22	11684.0
23	13311.0
24	15069.0
25	17277.0
26	18241.0
27	18685.0
28	19661.0
29	22427.0
30	26709.0
31	32972.0
32	41303.0
33	49608.0
34	54277.0
35	51667.0
36	56177.0
37	53576.0
38	30198.0
39	3036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.79801846830081	21.36225873345031	22.326793215292177	20.5129295829567
2	30.0070895588522	32.57935874940182	24.988036369436912	12.425515322309069
3	23.70571241204516	31.896634231934918	32.9678665745024	11.429786781517521
4	20.70328423813828	26.751741372893072	39.4766133177363	13.068361071232342
5	24.904379574980947	29.4092625086404	31.538611509898796	14.147746406479857
6	16.282767054820013	41.761932613743106	30.77612945534464	11.179170876092236
7	65.4511618014569	8.297265202672763	23.30657024866628	2.945002747204055
8	63.21954591375552	15.19168394746637	16.17234717569699	5.416422963081122
9	59.7561191754843	9.398450931390794	17.376154268800622	13.469275624324275
10	38.4826571666578	26.652842026904878	22.541075131599936	12.323425674837383
11	33.6115985182822	25.645238475035892	27.800641605076127	12.942521401605786
12	29.57161340635579	20.295634604136758	32.598855036245375	17.533896953262083
13	21.856755463391288	30.43370376278336	34.47971499973414	13.229825774091209
14	14.80087201573882	39.024476701937225	28.72086634409174	17.453784938232218
15	10.629021109161481	24.478474326935007	49.99680969851651	14.895694865387002
16	11.469133833147232	25.86926853476542	39.476436078765	23.185161553322345
17	12.588043458995765	24.201095336842666	35.28615231917194	27.92470888498963
18	16.333102922670637	24.6939969160419	37.57572535049006	21.3971748107974
19	19.17956080182911	27.21132202548696	32.4478474326935	21.16126973999043
20	22.839191081334963	24.70427677637759	35.65091012211765	16.805622020169793
21	18.464933269527304	32.74649509934244	32.78495595611563	16.003615675014622
22	17.446518140408713	25.324613175945128	31.97142907782563	25.257439605820526
23	16.970808741426065	32.49339784831889	31.484730862622072	19.051062547632974
24	18.468832526896016	24.965527020081176	33.97440669254356	22.591233760479255
25	14.751245103773417	32.03310823983978	32.741709647117204	20.473937009269598
26	16.515127346200885	29.29476613317736	32.940749011892734	21.24935750872902
27	17.76732067847078	32.69066482338137	30.198862125804222	19.34315237234363
28	15.75654454901544	28.58545577001471	38.60335690611652	17.054642774853335
29	20.660924123996384	26.07982843267578	32.603286010527995	20.655961432799845
30	17.51617305613158	28.646071498201024	35.67767320678471	18.160082238882687
31	25.516385742897146	25.997766788961556	27.51989507452899	20.96595239361231
32	27.816593112493575	27.029829318870636	27.109055139043974	18.04452242959182
33	21.779833749844915	25.048652097623226	31.351801634143317	21.819712518388542
34	25.521880151007604	23.01199907835735	31.720458694457736	19.745662076177307
35	20.214990872192978	22.291345421031174	31.627053756579997	25.86660995019585
36	29.40961698658301	21.28019708973609	31.8287517059251	17.4814342177558
37	24.79874514808316	23.687102320058134	34.26029315325854	17.25385937860017
38	25.12592828911221	24.876907534428668	28.815689193739917	21.1814749827192
39	26.660640541642294	21.44467485510714	32.812959713581826	19.08172488966874
40	24.003651122808883	19.87752787082824	32.98594494957551	23.132876056787367
41	18.198011378741956	22.007231350029244	34.59031211782847	25.20444515340033
42	23.477960333918222	20.777724606086387	31.299870615550944	24.444444444444443
43	23.95544212261392	20.949646408252246	32.70395774622924	22.39095372290459
44	22.977791956895484	22.40123358324028	32.79665372822176	21.824320731642473
45	19.377536732776804	24.740433526523812	30.444692579004272	25.437337161695112
46	24.258343524574183	25.72641392389359	31.42695095797664	18.588291593555592
47	18.711472678612573	21.72187660622818	39.634887719111674	19.93176299604757
48	21.002995338615055	22.575636731004415	31.76370500345616	24.657662926924374
49	19.631165700714273	19.32471951932791	37.728328104783685	23.315786675174138
50	20.03296644866273	23.04709239467574	32.733733893408484	24.186207263253042
51	18.6770883181794	23.800358022722037	27.831835664025807	29.690717995072756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	271.0
2	422.0
3	4214.5
4	8007.0
5	6848.0
6	5689.0
7	6034.0
8	6379.0
9	7046.0
10	7713.0
11	7921.5
12	8130.0
13	8245.5
14	8361.0
15	8343.0
16	8325.0
17	8206.5
18	8088.0
19	7825.0
20	7562.0
21	7260.5
22	6959.0
23	6310.5
24	5662.0
25	5211.0
26	4560.0
27	4360.0
28	5085.5
29	5811.0
30	6008.5
31	6206.0
32	6894.5
33	7583.0
34	8269.0
35	8955.0
36	10046.5
37	11138.0
38	12969.0
39	14800.0
40	19000.0
41	23200.0
42	29030.0
43	34860.0
44	39734.5
45	44609.0
46	66633.0
47	88657.0
48	71896.0
49	55135.0
50	51007.0
51	46879.0
52	39737.0
53	32595.0
54	27860.0
55	23125.0
56	20184.5
57	17244.0
58	16775.0
59	16306.0
60	15127.0
61	13948.0
62	12033.0
63	10118.0
64	7742.0
65	5366.0
66	4141.5
67	2917.0
68	2245.5
69	1574.0
70	1267.5
71	961.0
72	858.5
73	756.0
74	599.5
75	315.0
76	187.0
77	191.0
78	195.0
79	128.0
80	61.0
81	44.5
82	28.0
83	17.0
84	6.0
85	4.5
86	3.0
87	3.0
88	3.0
89	1.5
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	564210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.07500126655139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.66024349074254	31.758536359123806
2	6.685756050694389	4.957488280946962
3	2.0903636534756207	2.3250070530048492
4	1.0383153440778816	1.5398217078706882
5	0.6037567408939234	1.1192140966665574
6	0.43867785117112706	0.9758389132666547
7	0.2638245062981255	0.6846905733605209
8	0.23159491949861963	0.6869105546990553
9	0.1800013502050633	0.6006195258150314
>10	1.8899824000178662	17.206743532366286
>50	0.7327194423305321	19.501737396465852
>100	0.1759891674526395	8.375961689994332
>500	0.0029250277138942854	0.721342693555897
>1k	0.004387541570841428	4.0981519001970454
>5k	4.8750461898238085E-4	1.3579172279342155
>10k+	9.750092379647617E-4	4.090018494732246
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGC	11529	2.043388100175467	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG	11100	1.967352581485617	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTC	7513	1.3315963914145441	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	4786	0.8482657166657805	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3169	0.5616703000655784	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT	2564	0.45444072242604705	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT	2549	0.45178213785647187	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTC	2447	0.4337037627833608	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGTTCTT	2148	0.38070931036316263	No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT	2034	0.3605040676343914	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTA	1614	0.28606369968628703	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	1363	0.2415767178887294	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGGTCTTCGTATGCCGTCTTC	805	0.1426773719005335	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGT	754	0.13363818436397795	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTAT	635	0.11254674677868169	No Hit
ACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	614	0.10882472838127648	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	613	0.10864748940997146	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	570	0.10102621364385601	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7723897130501054E-4	0.0	0.0	0.4730508144130732	0.0
2	1.7723897130501054E-4	0.0	0.0	2.1426419241062726	0.0
3	1.7723897130501054E-4	0.0	0.0	2.448379149607416	0.0
4	1.7723897130501054E-4	0.0	0.0	3.396962124031832	0.0
5	1.7723897130501054E-4	0.0	0.0	6.168270679356977	0.0
6	1.7723897130501054E-4	0.0	0.0	6.817851509189841	0.0
7	1.7723897130501054E-4	0.0	0.0	7.803477428617004	0.0
8	1.7723897130501054E-4	0.0	0.0	9.358394923875862	0.0
9	1.7723897130501054E-4	0.0	0.0	9.866184576664716	0.0
10	1.7723897130501054E-4	0.0	0.0	13.315254958260223	0.0
11	1.7723897130501054E-4	0.0	0.0	14.292727885007356	0.0
12	1.7723897130501054E-4	0.0	0.0	17.9730951241559	0.0
13	1.7723897130501054E-4	0.0	0.0	18.803814182662485	0.0
14	1.7723897130501054E-4	0.0	0.0	19.137732404601124	0.0
15	1.7723897130501054E-4	0.0	0.0	19.848992396448132	0.0
16	1.7723897130501054E-4	0.0	0.0	20.67598943655731	0.0
17	1.7723897130501054E-4	0.0	0.0	21.42411513443576	0.0
18	1.7723897130501054E-4	0.0	0.0	23.145814501692634	0.0
19	1.7723897130501054E-4	0.0	0.0	23.931160383545134	0.0
20	1.7723897130501054E-4	0.0	0.0	24.60307332376243	0.0
21	1.7723897130501054E-4	0.0	0.0	24.873539993973875	0.0
22	1.7723897130501054E-4	0.0	0.0	25.126814483968737	0.0
23	1.7723897130501054E-4	0.0	0.0	25.295900462593714	0.0
24	1.7723897130501054E-4	0.0	0.0	25.44602187128906	0.0
25	1.7723897130501054E-4	0.0	0.0	25.569380195317347	0.0
26	1.7723897130501054E-4	0.0	0.0	25.69362471420216	0.0
27	1.7723897130501054E-4	0.0	0.0	25.870686446535863	0.0
28	1.7723897130501054E-4	0.0	0.0	26.071143723081832	0.0
29	3.544779426100211E-4	0.0	0.0	26.229063646514597	0.0
30	3.544779426100211E-4	0.0	0.0	26.395668279541304	0.0
31	3.544779426100211E-4	0.0	0.0	26.54649864412187	0.0
32	3.544779426100211E-4	0.0	0.0	26.68332712996934	0.0
33	3.544779426100211E-4	0.0	0.0	26.793569770121053	0.0
34	3.544779426100211E-4	0.0	0.0	26.928625866255473	0.0
35	3.544779426100211E-4	0.0	0.0	27.042236046861984	0.0
36	3.544779426100211E-4	0.0	0.0	27.146629800960635	0.0
37	3.544779426100211E-4	0.0	0.0	27.25013736020276	0.0
38	3.544779426100211E-4	0.0	0.0	27.34992290104748	0.0
39	3.544779426100211E-4	0.0	0.0	27.46052001914181	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTATA	30	2.1635296E-6	45.000004	16
CCCTAAT	30	2.1635296E-6	45.000004	40
CGGTTGA	30	2.1635296E-6	45.000004	30
GCCGATC	30	2.1635296E-6	45.000004	9
CGCTATT	30	2.1635296E-6	45.000004	30
AGCGAAG	30	2.1635296E-6	45.000004	1
TGAGGGT	75	0.0	45.000004	4
GACGCAC	30	2.1635296E-6	45.000004	9
TCCCCGT	30	2.1635296E-6	45.000004	24
GTAAGGT	30	2.1635296E-6	45.000004	24
ATCGGCC	30	2.1635296E-6	45.000004	15
AGGTGTC	30	2.1635296E-6	45.000004	22
AATCGAG	30	2.1635296E-6	45.000004	1
TGATCCA	20	7.0302305E-4	45.0	28
AAGTATC	20	7.0302305E-4	45.0	21
GTGCAGT	20	7.0302305E-4	45.0	3
CTATGGT	20	7.0302305E-4	45.0	28
AGACTGC	35	1.2104647E-7	45.0	37
GTCGCGG	20	7.0302305E-4	45.0	36
CGAACGT	20	7.0302305E-4	45.0	17
>>END_MODULE