FastQCFastQC Report
Sat 18 Jun 2016
SRR3548217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462913
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG58171.256607613093605No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC55971.209082484181693No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC49321.0654269808797765No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31920.6895464158491985No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC24510.5294731407413488TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT16370.35363016376727374Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGTCCCT16370.35363016376727374No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT15880.3430450214187115No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC15400.33267590238338524TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT13700.2959519391332713No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTC13370.2888231697964845No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTA9290.20068565799621096No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC7360.15899315854166982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6390.13803889715778128No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC5800.12529352167685937No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC5660.12226919529155586TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT5440.11751668240036464No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAC207.028915E-445.00000419
TTAGGTC207.028915E-445.00000421
TTAGGTA207.028915E-445.00000429
AACCGAA207.028915E-445.00000410
TTGGCGA207.028915E-445.00000424
CTATCGT207.028915E-445.00000413
ACGTTAG207.028915E-445.00000418
CGTGCCT207.028915E-445.0000045
TAGACGT207.028915E-445.00000438
CGGCCCA207.028915E-445.00000424
ACACCGA207.028915E-445.00000430
TAGGTCA207.028915E-445.00000422
TTATGCA207.028915E-445.00000425
GCTATCA207.028915E-445.0000049
ATAGCCG207.028915E-445.0000041
ACCGAAC207.028915E-445.00000411
ATTCGTA207.028915E-445.00000410
TTGTACG650.045.0000041
ATAGACT207.028915E-445.00000424
TACGGTA207.028915E-445.00000422
Produced by FastQC (version 0.11.5)