FastQCFastQC Report
Sat 18 Jun 2016
SRR3548212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438420
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGC148273.3819168833538615No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG133893.0539208977692622No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC124982.850691118105926No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC40250.9180694311390905No Hit
GAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT40130.9153323297294831No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTC33090.7547557136991926No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29160.6651156425345559No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT25200.5747912960175174No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTA19940.4548150175630674No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTGTATT19800.4516217325851923No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT12960.2956069522375804No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGT8430.19228137402490764No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT8070.18407006979608595No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC7860.1792801423292733No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC6520.14871584325532594No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTAT6400.1459787418457187No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTT5270.120204370238584No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC4910.11199306600976233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC453.8380676E-1045.00000425
TATCATC253.886844E-545.00000415
CTTCGGA253.886844E-545.00000413
AAATCTC253.886844E-545.00000427
CGCATTG253.886844E-545.00000427
GACGTAG253.886844E-545.0000041
GGGTACG253.886844E-545.0000047
ATAGCGG502.1827873E-1145.0000042
CGCGCAT253.886844E-545.00000425
GCCGGAT253.886844E-545.0000049
GTTAGTA253.886844E-545.00000442
TTCGTAG502.1827873E-1145.0000041
ATAGACC253.886844E-545.00000415
CTAAGAG253.886844E-545.0000041
CACGACG453.8380676E-1045.00000426
ACGGGTA253.886844E-545.0000045
AGAACCG253.886844E-545.0000041
AGTATGA253.886844E-545.00000433
ATACTCG302.1624182E-645.00000444
CGTTAGT253.886844E-545.00000441