##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438420
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.782966105560877	28.0	25.0	31.0	16.0	33.0
2	27.632993476574974	30.0	25.0	31.0	16.0	33.0
3	27.55689521463437	30.0	25.0	31.0	16.0	33.0
4	30.81117193558688	33.0	28.0	35.0	19.0	37.0
5	32.02063090187492	33.0	32.0	35.0	26.0	37.0
6	31.201596642488937	35.0	30.0	35.0	17.0	37.0
7	32.02157976369691	35.0	31.0	35.0	26.0	35.0
8	32.847185347383785	35.0	32.0	35.0	28.0	37.0
9	34.10087359153323	35.0	32.0	37.0	27.0	39.0
10	33.76522512659094	35.0	32.0	37.0	27.0	39.0
11	33.92383787235984	35.0	32.0	39.0	27.0	39.0
12	33.35054970119976	35.0	31.0	37.0	25.0	39.0
13	33.507615984672235	35.0	32.0	38.0	25.0	39.0
14	34.26970484923133	37.0	32.0	39.0	25.0	40.0
15	35.030721682405	37.0	33.0	39.0	27.0	40.0
16	35.11346197709958	36.0	33.0	39.0	27.0	41.0
17	34.5373044112951	36.0	32.0	39.0	27.0	40.0
18	34.444861092103466	36.0	32.0	39.0	27.0	40.0
19	33.798045253409974	36.0	32.0	38.0	25.0	40.0
20	33.47491674649879	35.0	32.0	38.0	25.0	40.0
21	34.1779024679531	35.0	32.0	39.0	27.0	40.0
22	34.69361799187993	36.0	33.0	39.0	27.0	40.0
23	34.871664157657044	36.0	33.0	39.0	28.0	40.0
24	34.65310204826422	36.0	33.0	39.0	27.0	40.0
25	33.95844395784864	35.0	32.0	39.0	24.0	40.0
26	33.87274303179599	35.0	32.0	39.0	24.0	40.0
27	34.24239085808129	35.0	33.0	39.0	25.0	40.0
28	33.938841749920165	35.0	33.0	39.0	24.0	40.0
29	34.05147347292551	36.0	33.0	39.0	24.0	40.0
30	33.41950869029697	35.0	32.0	39.0	23.0	40.0
31	33.55593951005885	35.0	32.0	39.0	23.0	40.0
32	33.032658181652295	35.0	31.0	39.0	20.0	40.0
33	32.84576433556863	35.0	31.0	39.0	18.0	40.0
34	32.605558596779346	35.0	31.0	39.0	15.0	40.0
35	32.273767163906754	35.0	31.0	39.0	14.0	40.0
36	32.143588339947996	35.0	31.0	39.0	12.0	40.0
37	31.902650426531636	35.0	30.0	39.0	10.0	40.0
38	31.823675927193104	35.0	30.0	39.0	10.0	40.0
39	31.674645317275672	35.0	30.0	39.0	10.0	40.0
40	31.24853565074586	35.0	29.0	39.0	10.0	40.0
41	30.924513024040873	35.0	28.0	38.0	10.0	40.0
42	31.321880844851968	35.0	30.0	39.0	10.0	40.0
43	31.162795949089915	35.0	29.0	39.0	10.0	40.0
44	31.351881757219104	35.0	30.0	39.0	10.0	40.0
45	31.3693832398157	35.0	30.0	39.0	10.0	40.0
46	31.103343825555402	35.0	29.0	38.0	10.0	40.0
47	31.111178778340403	35.0	29.0	38.0	10.0	40.0
48	30.90967337256512	35.0	28.0	38.0	9.0	40.0
49	31.042892660006387	35.0	29.0	38.0	10.0	40.0
50	30.806256557638793	35.0	28.0	38.0	8.0	40.0
51	28.36634505725104	32.0	23.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	14.0
10	23.0
11	13.0
12	24.0
13	21.0
14	66.0
15	124.0
16	313.0
17	797.0
18	1573.0
19	2606.0
20	3868.0
21	5118.0
22	6917.0
23	8408.0
24	10671.0
25	13084.0
26	14139.0
27	14465.0
28	15405.0
29	17681.0
30	21122.0
31	26080.0
32	32842.0
33	39539.0
34	43589.0
35	43002.0
36	46519.0
37	43648.0
38	24429.0
39	2311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.592354363395835	18.53656311299667	20.972127183978834	19.898955339628664
2	31.96250171068838	34.476529355412616	22.346380183385794	11.214588750513206
3	22.49327129236805	34.425436795766615	32.413667259705306	10.667624652160029
4	19.552940103097484	22.75808585374755	44.19894165412162	13.490032389033345
5	28.24734273071484	23.89238629624561	33.13854294968295	14.721728023356597
6	17.75762967017928	41.8571233064185	30.47579946170339	9.909447561698828
7	62.568085397563976	5.504767118288399	28.910633638976325	3.016513845171297
8	58.537247388349066	15.49952100725332	19.482459741800103	6.480771862597509
9	55.16855982847498	6.138178002828338	20.088955795812236	18.604306372884448
10	39.22699694357009	21.013867980475343	25.13799552940103	14.621139546553533
11	33.75484695041285	20.658044797226406	30.274394416313122	15.312713836047626
12	27.559417909766893	16.525705944071895	35.17380593951006	20.74107020665116
13	19.577117832215684	26.77067652023174	40.00182473427307	13.650380913279502
14	15.756808539756397	34.56274804981525	31.519775557684408	18.160667852743945
15	10.679713516719127	20.674239313899914	54.01555585967793	14.630491309703025
16	11.829524200538296	21.738515578668856	40.68974955522102	25.742210665571825
17	12.024770767756946	21.955202773596096	38.637835865152134	27.38219059349482
18	15.01094840563843	22.90611742165047	39.68956708179371	22.393367090917383
19	17.540714383467908	26.492176451804205	33.2181469823457	22.74896218238219
20	21.286893846083665	23.133753022216137	38.999589434788554	16.579763696911638
21	16.854158113224763	31.483508964007118	35.10857168924775	16.55376123352037
22	15.834587838146069	22.600930614479267	35.2016331371744	26.36284841020026
23	15.700241777291183	32.39633228411113	33.219971716618765	18.683454221978923
24	18.39514620683363	21.72528625518909	35.18452625336435	24.695041284612927
25	13.282012681903199	32.14885269832581	33.46357374207381	21.105560877697187
26	15.43702385840062	25.612654532183747	35.357876009306146	23.592445600109485
27	19.681355777564892	28.418411568815294	30.922859358605905	20.977373295013912
28	13.238447151133617	25.698645134802245	40.3400848501437	20.72282286392044
29	21.472560558368688	19.666985995164453	35.78691665526208	23.07353679120478
30	17.145431321563798	24.940239952556908	38.92226631996715	18.99206240591214
31	23.52561470735824	22.14953697367821	29.319830299712606	25.005018019250947
32	27.05830026002463	25.832991195657133	31.451576114228363	15.657132430089868
33	22.74873409059806	21.896583185073673	30.405319100406004	24.949363623922267
34	24.08717667989599	22.602527256968205	33.0299712604352	20.280324802700605
35	17.734136216413486	24.095616075908946	34.500935176314954	23.66931253136262
36	26.94904429542448	20.20277359609507	32.145659413347936	20.702522695132522
37	18.3579672460198	22.26221431504037	42.467496920760915	16.912321518178917
38	22.414807718625976	25.87381962501711	26.7052141781853	25.006158478171614
39	22.906345513434605	20.658729072578804	38.17686236941745	18.258063044569134
40	24.93111628119155	23.101592080653255	33.16272067880115	18.804570959354045
41	16.26659367729574	23.377811231239452	36.24515304958715	24.110442041877654
42	20.732858902422336	24.592856165320924	33.70694767574472	20.96733725651202
43	22.86141143196022	21.254960996304913	33.4339218101364	22.44970576159847
44	20.64618402445144	22.287304411295107	33.68436658911546	23.382144975137994
45	17.71588887368277	22.554399890515946	31.3268099083071	28.402901327494185
46	26.92007663883947	25.077779298389675	29.382327448565306	18.619816614205558
47	18.43871173760321	20.363350212125358	40.52027735960951	20.677660690661924
48	21.40299256420784	23.152228456730988	30.84439578486383	24.600383194197345
49	18.72017699922449	19.346745130240407	39.03973358879613	22.893344281738973
50	20.991514985630218	21.151863509876375	33.67113726563569	24.185484238857715
51	19.2431914602436	20.560193421832945	28.242096619679756	31.954518498243694
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	58.0
1	162.0
2	266.0
3	3732.0
4	7198.0
5	6033.0
6	4868.0
7	5110.5
8	5353.0
9	5575.0
10	5797.0
11	5861.0
12	5925.0
13	5714.5
14	5504.0
15	5189.0
16	4874.0
17	4658.0
18	4442.0
19	4278.5
20	4115.0
21	4001.5
22	3888.0
23	3483.5
24	3079.0
25	2995.5
26	3057.0
27	3202.0
28	3289.0
29	3376.0
30	3654.5
31	3933.0
32	5029.0
33	6125.0
34	6177.0
35	6229.0
36	7274.0
37	8319.0
38	9593.0
39	10867.0
40	15643.0
41	20419.0
42	27153.0
43	33887.0
44	63131.5
45	92376.0
46	67977.0
47	43578.0
48	40086.0
49	36594.0
50	31286.5
51	25979.0
52	23441.5
53	20904.0
54	17347.0
55	13790.0
56	13277.0
57	12764.0
58	12255.0
59	11746.0
60	10943.0
61	10140.0
62	8324.0
63	6508.0
64	5157.0
65	3806.0
66	2971.0
67	2136.0
68	1671.0
69	1206.0
70	1037.5
71	869.0
72	737.0
73	605.0
74	443.5
75	244.5
76	207.0
77	189.5
78	172.0
79	115.0
80	58.0
81	41.0
82	24.0
83	22.5
84	21.0
85	19.0
86	17.0
87	9.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	438420.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.49108816509241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.97406011062063	29.803307709668058
2	7.4937150992427135	5.319202065426146
3	2.454823796241033	2.61373103346472
4	1.1606395880502407	1.647694477895505
5	0.708180297716538	1.2567044691519518
6	0.44526904727188193	0.9481849808347838
7	0.29265315756802485	0.7270605311927614
8	0.2440812881753717	0.6930168414465155
9	0.18918948694851373	0.604308668507847
>10	2.248860145002354	19.650965053141423
>50	0.6933045873464974	16.8405030841819
>100	0.08413574013894465	4.381188171793364
>500	0.0039132902390206815	0.9849398040355453
>1k	0.0052177203186942415	5.104789071303379
>5k	0.0	0.0
>10k+	0.0019566451195103408	9.424404037956096
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGC	14827	3.3819168833538615	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG	13389	3.0539208977692622	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	12498	2.850691118105926	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	4025	0.9180694311390905	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	4013	0.9153323297294831	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTC	3309	0.7547557136991926	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2916	0.6651156425345559	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT	2520	0.5747912960175174	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTA	1994	0.4548150175630674	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTGTATT	1980	0.4516217325851923	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	1296	0.2956069522375804	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGT	843	0.19228137402490764	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT	807	0.18407006979608595	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	786	0.1792801423292733	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC	652	0.14871584325532594	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTAT	640	0.1459787418457187	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTT	527	0.120204370238584	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	491	0.11199306600976233	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36471876283016286	0.0
2	0.0	0.0	0.0	1.9045663975183613	0.0
3	0.0	0.0	0.0	2.2284567309885497	0.0
4	0.0	0.0	0.0	3.663610236759272	0.0
5	0.0	0.0	0.0	7.969526937639706	0.0
6	0.0	0.0	0.0	8.945531681948816	0.0
7	0.0	0.0	0.0	10.172209297021121	0.0
8	0.0	0.0	0.0	12.444459650563386	0.0
9	0.0	0.0	0.0	13.037270197527485	0.0
10	0.0	0.0	0.0	17.57675288536107	0.0
11	0.0	0.0	0.0	18.976095981022763	0.0
12	0.0	0.0	0.0	24.193467451302404	0.0
13	0.0	0.0	0.0	25.54582363943251	0.0
14	0.0	0.0	0.0	25.980338488207654	0.0
15	0.0	0.0	0.0	26.846174900780074	0.0
16	0.0	0.0	0.0	27.670954792208384	0.0
17	0.0	0.0	0.0	28.277222754436384	0.0
18	2.280917841339355E-4	0.0	0.0	29.47082706080927	0.0
19	2.280917841339355E-4	0.0	0.0	30.380000912367137	0.0
20	2.280917841339355E-4	0.0	0.0	30.90757720906893	0.0
21	2.280917841339355E-4	0.0	0.0	31.24560923315542	0.0
22	2.280917841339355E-4	0.0	0.0	31.626522512659093	0.0
23	2.280917841339355E-4	0.0	0.0	31.931937411614435	0.0
24	2.280917841339355E-4	0.0	0.0	32.19834861548287	0.0
25	2.280917841339355E-4	0.0	0.0	32.387208612745766	0.0
26	2.280917841339355E-4	0.0	0.0	32.59591259522832	0.0
27	2.280917841339355E-4	0.0	0.0	32.81898636011131	0.0
28	2.280917841339355E-4	0.0	0.0	33.005565439532866	0.0
29	2.280917841339355E-4	0.0	0.0	33.20218055745632	0.0
30	2.280917841339355E-4	0.0	0.0	33.445098307558965	0.0
31	2.280917841339355E-4	0.0	0.0	33.661785502486204	0.0
32	2.280917841339355E-4	0.0	0.0	33.85908489576205	0.0
33	2.280917841339355E-4	0.0	0.0	34.04520779161535	0.0
34	2.280917841339355E-4	0.0	0.0	34.26121071119018	0.0
35	2.280917841339355E-4	0.0	0.0	34.472423703298205	0.0
36	2.280917841339355E-4	0.0	0.0	34.66128370056111	0.0
37	4.56183568267871E-4	0.0	0.0	34.85037178960814	0.0
38	4.56183568267871E-4	0.0	0.0	35.03193284977875	0.0
39	4.56183568267871E-4	0.0	0.0	35.208475890698416	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	45	3.8380676E-10	45.000004	25
TATCATC	25	3.886844E-5	45.000004	15
CTTCGGA	25	3.886844E-5	45.000004	13
AAATCTC	25	3.886844E-5	45.000004	27
CGCATTG	25	3.886844E-5	45.000004	27
GACGTAG	25	3.886844E-5	45.000004	1
GGGTACG	25	3.886844E-5	45.000004	7
ATAGCGG	50	2.1827873E-11	45.000004	2
CGCGCAT	25	3.886844E-5	45.000004	25
GCCGGAT	25	3.886844E-5	45.000004	9
GTTAGTA	25	3.886844E-5	45.000004	42
TTCGTAG	50	2.1827873E-11	45.000004	1
ATAGACC	25	3.886844E-5	45.000004	15
CTAAGAG	25	3.886844E-5	45.000004	1
CACGACG	45	3.8380676E-10	45.000004	26
ACGGGTA	25	3.886844E-5	45.000004	5
AGAACCG	25	3.886844E-5	45.000004	1
AGTATGA	25	3.886844E-5	45.000004	33
ATACTCG	30	2.1624182E-6	45.000004	44
CGTTAGT	25	3.886844E-5	45.000004	41
>>END_MODULE