SRR3548210_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1297665
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	39647	3.05525694227709	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	35031	2.699541098819803	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	30579	2.3564633399220907	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	9243	0.7122793633179596	TruSeq Adapter, Index 27 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	8747	0.674056863674369	TruSeq Adapter, Index 27 (100% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC	7402	0.5704091579876163	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6800	0.5240181402750325	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	6279	0.48386910335101896	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACCTTTCT	4672	0.36003128696543407	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA	4557	0.3511692154754887	No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2058	0.15859254892441424	TruSeq Adapter, Index 27 (100% over 25bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGT	1888	0.14549209541753844	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTAT	1569	0.12090947971934204	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1515	0.11674815919362855	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	70	0.0	45.000004	26
CGACAGT	35	1.2120836E-7	45.000004	37
GCCGATC	35	1.2120836E-7	45.000004	24
TTGACGT	35	1.2120836E-7	45.000004	29
GAACGTA	70	0.0	45.000004	9
ACTCGAA	35	1.2120836E-7	45.000004	7
TACCGAA	35	1.2120836E-7	45.000004	12
CGGACTA	35	1.2120836E-7	45.000004	15
CTTAACG	75	0.0	45.0	1
AACGTAC	25	3.8910854E-5	45.0	37
ATCTCGC	25	3.8910854E-5	45.0	12
CGAACGA	25	3.8910854E-5	45.0	14
GCGACTT	20	7.033623E-4	45.0	43
GTCGAGT	25	3.8910854E-5	45.0	13
GTCGACA	40	6.8157533E-9	45.0	41
CGACGTG	25	3.8910854E-5	45.0	20
GGTCGAC	20	7.033623E-4	45.0	36
CGACGAT	25	3.8910854E-5	45.0	32
CTATCGG	25	3.8910854E-5	45.0	2
CGTGCGG	40	6.8157533E-9	45.0	2