##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1297665
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.53227682028875	28.0	25.0	31.0	16.0	33.0
2	27.422539715566035	30.0	25.0	31.0	16.0	33.0
3	27.460132622826386	30.0	25.0	31.0	16.0	33.0
4	30.87914908701398	33.0	28.0	35.0	19.0	37.0
5	31.899961854561848	33.0	32.0	35.0	26.0	37.0
6	31.036704388266617	35.0	30.0	35.0	17.0	37.0
7	31.727649277741172	33.0	30.0	35.0	25.0	35.0
8	33.023004396358075	35.0	32.0	35.0	28.0	37.0
9	34.17178393499093	35.0	32.0	37.0	27.0	39.0
10	33.57145796488308	35.0	32.0	37.0	27.0	39.0
11	33.75619131285809	35.0	32.0	39.0	26.0	39.0
12	33.27556495705748	35.0	31.0	37.0	25.0	39.0
13	33.33099991137929	35.0	32.0	37.0	25.0	39.0
14	34.1221732881753	36.0	32.0	39.0	24.0	40.0
15	34.95865805119195	37.0	33.0	39.0	27.0	40.0
16	35.135792365518064	37.0	33.0	39.0	27.0	41.0
17	34.61594016945822	36.0	33.0	39.0	27.0	40.0
18	34.52253547718402	36.0	33.0	39.0	27.0	40.0
19	34.001642950992746	36.0	32.0	39.0	25.0	40.0
20	33.86708202810433	35.0	32.0	38.0	25.0	40.0
21	34.409178023603936	36.0	33.0	39.0	27.0	40.0
22	34.7654248207357	36.0	33.0	39.0	27.0	40.0
23	35.10671783549684	36.0	33.0	39.0	29.0	40.0
24	34.85479996763417	36.0	33.0	39.0	27.0	40.0
25	34.14321261650734	35.0	33.0	39.0	25.0	40.0
26	34.098419083507686	36.0	32.0	39.0	25.0	40.0
27	34.53692748128369	36.0	33.0	39.0	26.0	40.0
28	34.39648137231104	36.0	33.0	39.0	25.0	40.0
29	34.43135169708669	36.0	33.0	39.0	25.0	40.0
30	34.039991831481935	36.0	33.0	39.0	24.0	40.0
31	34.183368588965564	36.0	33.0	39.0	24.0	40.0
32	33.7313112398038	36.0	33.0	39.0	23.0	40.0
33	33.68248507896876	36.0	33.0	39.0	22.0	40.0
34	33.64106067436511	36.0	33.0	39.0	21.0	40.0
35	33.60148651616557	37.0	33.0	40.0	19.0	40.0
36	33.38757306392636	37.0	33.0	40.0	18.0	40.0
37	33.32030840008785	37.0	33.0	40.0	18.0	40.0
38	33.287529524183824	36.0	32.0	40.0	18.0	40.0
39	33.00020421295172	36.0	32.0	39.0	16.0	40.0
40	32.717966501369766	36.0	31.0	39.0	15.0	40.0
41	32.4691511291435	36.0	31.0	39.0	15.0	40.0
42	32.8288633815353	36.0	32.0	39.0	15.0	40.0
43	32.725706557547596	36.0	31.0	39.0	15.0	40.0
44	32.63357106803374	36.0	31.0	39.0	15.0	40.0
45	32.72074148566849	36.0	32.0	39.0	15.0	40.0
46	32.31486477634829	35.0	31.0	39.0	15.0	40.0
47	32.3503531342835	35.0	31.0	39.0	15.0	40.0
48	32.20991935514944	35.0	31.0	39.0	13.0	40.0
49	32.36655531281186	35.0	31.0	39.0	14.0	40.0
50	32.2043439562599	35.0	31.0	39.0	13.0	40.0
51	30.105528006072447	34.0	27.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	39.0
9	88.0
10	82.0
11	70.0
12	57.0
13	106.0
14	150.0
15	389.0
16	803.0
17	1634.0
18	3164.0
19	5448.0
20	8066.0
21	11090.0
22	15086.0
23	19701.0
24	25175.0
25	30871.0
26	35103.0
27	38008.0
28	41280.0
29	48262.0
30	60356.0
31	75987.0
32	96548.0
33	118577.0
34	132989.0
35	133633.0
36	149782.0
37	146845.0
38	88708.0
39	9565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.58096658228433	18.72748359553506	21.669306022740848	19.022243799439764
2	30.488454262078424	32.91697009628833	23.86725387522974	12.727321766403502
3	24.660833111781546	31.94260460134164	31.596752628760115	11.799809658116695
4	22.459109246223026	22.53840552068523	40.997715126785415	14.004770106306328
5	28.396543021504012	25.447553875615046	30.327858114382373	15.828044988498572
6	17.1884114929508	41.07439131054625	31.197728227238926	10.539468969264023
7	69.92051107180976	3.640770152543222	24.06576427660452	2.372954499042511
8	67.26851691307078	12.372684783823251	15.520492577051858	4.838305726054105
9	63.89476482759417	4.315751754112194	16.152627989504225	15.636855428789403
10	39.79925481537993	23.628440313948516	23.0589558938555	13.51334897681605
11	35.19352066981848	21.18335625912697	28.451641987724102	15.171481083330443
12	29.26571958093961	18.783815545614623	32.73487379254276	19.215591080903007
13	20.014872867804865	29.036692829042938	38.033236621161855	12.915197681990346
14	17.418131798268426	34.65940747419403	29.758296632798142	18.164164094739395
15	13.70754393468268	21.71847125413724	49.98562803188805	14.588356779292036
16	14.255605260217392	22.34698477650241	39.66401189829424	23.733398064985956
17	14.364416085815677	22.07734661873442	36.720648241264115	26.83758905418579
18	17.355172559944208	22.868459887567287	38.45722894583733	21.31913860665118
19	20.49905021712075	25.29751515221571	32.726011721052814	21.477422909610723
20	23.78179268147018	23.06874270324005	37.11304535454066	16.036419260749117
21	19.805419734677283	30.65544651354548	32.49120535731487	17.04792839446236
22	17.873102842413104	21.82389137412198	34.74741169716375	25.555594086301163
23	17.828792484963376	31.276176825297746	31.921181506783334	18.97384918295554
24	20.37837192187506	22.439535627453928	34.50428269237438	22.677809758296632
25	14.916715793367317	30.704765867924312	33.77782401467251	20.600694324035864
26	15.55070068160889	26.190657835419774	33.26960348009694	24.989038002874395
27	19.16750471038365	31.017250214808907	30.13705386212929	19.678191212678158
28	14.059714949543988	26.34570555574821	39.34150955755145	20.253069937156354
29	18.01058054274408	23.085465046834123	33.32824727491301	25.57570713550878
30	16.655454219694604	30.574377824785287	33.9363395021057	18.8338284534144
31	23.594610319304287	23.24837303926668	28.181695583991246	24.97532105743778
32	23.913105462503808	27.081796919852195	28.904994740553224	20.100102877090777
33	20.32566186188269	24.698593242477834	29.584985339051293	25.390759556588176
34	20.1284615058586	23.55962440229181	32.327141442514055	23.984772649335536
35	16.104695741967305	25.944831678437808	32.01658363290988	25.93388894668501
36	21.13634874948465	21.885694690077948	28.397853066854694	28.580103493582705
37	16.461798692266495	28.652387172344174	33.56582785233477	21.31998628305456
38	19.02478682864992	26.402345751792645	28.22754717126531	26.345320248292126
39	20.739019700770232	23.870644580843283	31.607310053056835	23.78302566532965
40	19.763267098981633	22.37056559281479	36.61700053557737	21.249166772626218
41	15.692031456500716	24.61790986117372	32.32089946172548	27.369159220600075
42	19.740919266528724	22.805577710734283	34.29814320336913	23.155359819367867
43	21.47426338847083	23.04901496148852	26.959192087326084	28.517529562714568
44	19.66308716039964	25.086983158211098	31.535488743242666	23.714440938146595
45	17.137242662782768	26.450432122312	29.113291951312547	27.29903326359268
46	24.874062257978753	27.518966759525764	27.51056705698312	20.096403925512362
47	18.009501681867047	23.86324667768646	37.456970789841755	20.67028085060474
48	21.934012245070956	24.92222568998933	29.192588225774756	23.95117383916496
49	18.905726824719785	21.281378475954888	36.340658028073506	23.472236671251824
50	20.29815090951825	24.91197651165748	31.201273055834903	23.58859952298937
51	19.136371867932016	24.700673902740693	26.712210007975862	29.450744221351428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	316.0
2	562.0
3	9201.0
4	17840.0
5	14862.0
6	11884.0
7	12260.5
8	12637.0
9	12943.0
10	13249.0
11	13305.0
12	13361.0
13	12626.5
14	11892.0
15	11243.0
16	10594.0
17	10266.0
18	9938.0
19	9660.0
20	9382.0
21	8824.5
22	8267.0
23	8340.0
24	8413.0
25	8414.5
26	9298.0
27	10180.0
28	12049.5
29	13919.0
30	17184.0
31	20449.0
32	21894.5
33	23340.0
34	25760.0
35	28180.0
36	31966.0
37	35752.0
38	39060.5
39	42369.0
40	52427.0
41	62485.0
42	75086.5
43	87688.0
44	95376.0
45	103064.0
46	173276.0
47	243488.0
48	179547.0
49	115606.0
50	107118.0
51	98630.0
52	84396.0
53	70162.0
54	60960.0
55	51758.0
56	46145.0
57	40532.0
58	37341.0
59	34150.0
60	31115.5
61	28081.0
62	25243.0
63	22405.0
64	17683.0
65	12961.0
66	10099.5
67	7238.0
68	5336.0
69	3434.0
70	2718.0
71	2002.0
72	1513.0
73	1024.0
74	992.0
75	856.5
76	753.0
77	578.0
78	403.0
79	245.5
80	88.0
81	54.0
82	20.0
83	23.0
84	26.0
85	15.0
86	4.0
87	4.5
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1297665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.25228722487933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.86097404275547	21.752900903036874
2	8.518841221091588	4.472781331184763
3	2.7410302883072895	2.1587494326221024
4	1.2217331380056495	1.2829315700430979
5	0.7026659586815998	0.9223294285227274
6	0.47631081493222044	0.7502548993150195
7	0.31973139364324377	0.5875576266513374
8	0.22755119873071591	0.4778991541955481
9	0.17590289776928622	0.4156068056335099
>10	1.4204224169653434	8.328206963670889
>50	0.5269724059727567	10.160438788133602
>100	0.797360787851403	34.92276361500193
>500	0.0054017667223653365	0.8823654405522743
>1k	0.0027008833611826683	1.561944395034767
>5k	0.0015004907562125934	3.0308465056684413
>10k+	9.00294453727556E-4	8.292423140733101
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	39647	3.05525694227709	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	35031	2.699541098819803	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	30579	2.3564633399220907	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	9243	0.7122793633179596	TruSeq Adapter, Index 27 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	8747	0.674056863674369	TruSeq Adapter, Index 27 (100% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC	7402	0.5704091579876163	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6800	0.5240181402750325	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	6279	0.48386910335101896	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACCTTTCT	4672	0.36003128696543407	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA	4557	0.3511692154754887	No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2058	0.15859254892441424	TruSeq Adapter, Index 27 (100% over 25bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGT	1888	0.14549209541753844	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTAT	1569	0.12090947971934204	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1515	0.11674815919362855	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.706149121691653E-5	0.0	0.0	0.20814308777689158	0.0
2	7.706149121691653E-5	0.0	0.0	1.3773970940111662	0.0
3	7.706149121691653E-5	0.0	0.0	1.560572258633777	0.0
4	7.706149121691653E-5	0.0	0.0	2.5619863369976072	0.0
5	7.706149121691653E-5	0.0	0.0	5.902370796777288	0.0
6	7.706149121691653E-5	0.0	0.0	6.374911860919421	0.0
7	7.706149121691653E-5	0.0	0.0	7.228676122111639	0.0
8	1.5412298243383307E-4	0.0	0.0	8.537026119992447	0.0
9	1.5412298243383307E-4	0.0	0.0	8.849741651350696	0.0
10	1.5412298243383307E-4	0.0	0.0	12.432484501007579	0.0
11	1.5412298243383307E-4	0.0	0.0	13.017997711273711	0.0
12	1.5412298243383307E-4	0.0	0.0	17.096091826472936	0.0
13	1.5412298243383307E-4	0.0	0.0	17.763598463393866	0.0
14	1.5412298243383307E-4	0.0	0.0	18.02691757888207	0.0
15	1.5412298243383307E-4	0.0	0.0	18.60888596055222	0.0
16	1.5412298243383307E-4	0.0	0.0	19.078883995484198	0.0
17	1.5412298243383307E-4	0.0	0.0	19.40192576666551	0.0
18	3.0824596486766614E-4	0.0	0.0	19.931107026852075	0.0
19	3.0824596486766614E-4	0.0	0.0	20.55106672369217	0.0
20	3.0824596486766614E-4	0.0	0.0	20.82101312742503	0.0
21	3.0824596486766614E-4	0.0	0.0	21.01543926976531	0.0
22	3.0824596486766614E-4	0.0	0.0	21.27683184797309	0.0
23	3.0824596486766614E-4	0.0	0.0	21.47803940154046	0.0
24	3.0824596486766614E-4	0.0	0.0	21.638558487745296	0.0
25	3.0824596486766614E-4	0.0	0.0	21.770487760708658	0.0
26	3.0824596486766614E-4	0.0	0.0	21.91320564244239	0.0
27	3.0824596486766614E-4	0.0	0.0	22.08351153803177	0.0
28	3.0824596486766614E-4	0.0	0.0	22.212666597311326	0.0
29	3.0824596486766614E-4	0.0	0.0	22.356617462904524	0.0
30	3.0824596486766614E-4	0.0	0.0	22.527308665949995	0.0
31	3.853074560845827E-4	0.0	0.0	22.688598367067	0.0
32	3.853074560845827E-4	0.0	0.0	22.83740410660687	0.0
33	3.853074560845827E-4	0.0	0.0	22.988213444918372	0.0
34	3.853074560845827E-4	0.0	0.0	23.14827016217591	0.0
35	3.853074560845827E-4	0.0	0.0	23.30239314460974	0.0
36	4.6236894730149924E-4	0.0	0.0	23.459675648183467	0.0
37	4.6236894730149924E-4	0.0	0.0	23.611409724389578	0.0
38	4.6236894730149924E-4	0.0	0.0	23.75659357384225	0.0
39	4.6236894730149924E-4	0.0	0.0	23.904320452505075	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	70	0.0	45.000004	26
CGACAGT	35	1.2120836E-7	45.000004	37
GCCGATC	35	1.2120836E-7	45.000004	24
TTGACGT	35	1.2120836E-7	45.000004	29
GAACGTA	70	0.0	45.000004	9
ACTCGAA	35	1.2120836E-7	45.000004	7
TACCGAA	35	1.2120836E-7	45.000004	12
CGGACTA	35	1.2120836E-7	45.000004	15
CTTAACG	75	0.0	45.0	1
AACGTAC	25	3.8910854E-5	45.0	37
ATCTCGC	25	3.8910854E-5	45.0	12
CGAACGA	25	3.8910854E-5	45.0	14
GCGACTT	20	7.033623E-4	45.0	43
GTCGAGT	25	3.8910854E-5	45.0	13
GTCGACA	40	6.8157533E-9	45.0	41
CGACGTG	25	3.8910854E-5	45.0	20
GGTCGAC	20	7.033623E-4	45.0	36
CGACGAT	25	3.8910854E-5	45.0	32
CTATCGG	25	3.8910854E-5	45.0	2
CGTGCGG	40	6.8157533E-9	45.0	2
>>END_MODULE