SRR3548208_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548208_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800555
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	24908	3.111341506829637	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	20690	2.5844570329334027	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	17761	2.2185858560623566	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	5672	0.7085084722473785	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	4946	0.6178213864131759	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4269	0.5332550543060752	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTC	4223	0.5275090406030816	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT	3645	0.4553091292915539	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGATGGCT	2768	0.3457601289105683	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTA	2532	0.316280580347384	No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	1149	0.14352542923346928	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGT	1044	0.13040952838967967	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTT	918	0.11467044737713211	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT	893	0.11154761384289649	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	850	0.1061763401640112	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	35	1.2113014E-7	45.000004	15
ATATCGT	35	1.2113014E-7	45.000004	12
GCACAAT	35	1.2113014E-7	45.000004	41
TCGTCGG	35	1.2113014E-7	45.000004	2
GATATCG	35	1.2113014E-7	45.000004	11
TATTACC	35	1.2113014E-7	45.000004	13
CGTACAG	65	0.0	45.000004	25
CGCGTTA	35	1.2113014E-7	45.000004	34
ATCGGGC	35	1.2113014E-7	45.000004	4
CCGTTTA	35	1.2113014E-7	45.000004	15
TAGTCCG	35	1.2113014E-7	45.000004	11
TCAGTAG	35	1.2113014E-7	45.000004	1
CAGACGT	115	0.0	45.000004	23
TGATCGG	25	3.8897408E-5	45.0	2
TGATCGC	20	7.0320035E-4	45.0	41
TTGATCG	25	3.8897408E-5	45.0	1
AATCCGA	20	7.0320035E-4	45.0	10
CTCACCG	20	7.0320035E-4	45.0	26
AGATCTC	20	7.0320035E-4	45.0	18
GTTCTCG	20	7.0320035E-4	45.0	11