SRR3548207_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548207_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520615
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	10257	1.9701698952200764	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	8683	1.667835156497604	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	7734	1.4855507428714116	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3422	0.6572995399671542	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	3215	0.6175388722952662	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGATGGCT	2392	0.4594566042084842	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	2259	0.43390989502799576	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTC	2049	0.3935729857956456	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT	1800	0.3457449362772874	No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	1732	0.3326834609068121	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTA	1498	0.28773661919076476	No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1037	0.19918749939974836	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	826	0.15865850964724412	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	800	0.15366441612323886	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT	704	0.1352246861884502	No Hit
ACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	663	0.1273493848621342	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGT	560	0.1075650912862672	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	538	0.10333931984287813	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	20	7.029725E-4	45.000004	43
AGGTACA	20	7.029725E-4	45.000004	15
ATCTCCG	20	7.029725E-4	45.000004	10
ACCGGTA	20	7.029725E-4	45.000004	22
GCGACTT	20	7.029725E-4	45.000004	9
CTACTTA	20	7.029725E-4	45.000004	25
TTGTGCG	40	6.8030204E-9	45.000004	1
TCCGAGT	20	7.029725E-4	45.000004	13
GCAACCG	20	7.029725E-4	45.000004	2
CGTTGAT	20	7.029725E-4	45.000004	25
CGTTGAA	20	7.029725E-4	45.000004	27
TGGAACG	20	7.029725E-4	45.000004	1
TAGCGAA	20	7.029725E-4	45.000004	43
CACGCTT	20	7.029725E-4	45.000004	18
CCAATGT	20	7.029725E-4	45.000004	38
GAATCGT	20	7.029725E-4	45.000004	25
GTGATAA	20	7.029725E-4	45.000004	9
CACGATC	20	7.029725E-4	45.000004	45
TAACGTG	20	7.029725E-4	45.000004	1
TCGATTG	40	6.8030204E-9	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content