##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548207_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 520615 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.286424709238112 30.0 25.0 31.0 16.0 33.0 2 27.773692651959703 30.0 25.0 33.0 16.0 34.0 3 27.646454673799255 30.0 25.0 33.0 16.0 34.0 4 32.01672445088981 35.0 32.0 35.0 19.0 37.0 5 33.071475082354524 35.0 32.0 35.0 28.0 37.0 6 31.85990223101524 35.0 32.0 35.0 25.0 37.0 7 33.78335430212345 35.0 33.0 35.0 31.0 37.0 8 33.51011015817831 35.0 33.0 37.0 28.0 37.0 9 36.17857918039242 39.0 35.0 39.0 31.0 39.0 10 35.2764346013849 37.0 34.0 39.0 30.0 39.0 11 35.46867838998108 37.0 34.0 39.0 30.0 39.0 12 35.42968796519501 37.0 34.0 39.0 30.0 39.0 13 35.19419148507054 37.0 34.0 39.0 30.0 39.0 14 35.83287266021917 38.0 34.0 40.0 27.0 41.0 15 36.4057297619162 38.0 35.0 40.0 31.0 41.0 16 36.23370436887143 37.0 34.0 40.0 32.0 41.0 17 35.742279803693705 37.0 34.0 39.0 30.0 41.0 18 35.87485954111964 37.0 34.0 39.0 30.0 40.0 19 35.35182428474016 37.0 34.0 39.0 29.0 40.0 20 35.45729953996715 37.0 34.0 39.0 29.0 40.0 21 35.838174082575414 37.0 34.0 39.0 30.0 40.0 22 36.263248273676325 38.0 35.0 40.0 31.0 41.0 23 36.44267068755222 38.0 35.0 40.0 31.0 41.0 24 36.42360093351133 38.0 35.0 40.0 31.0 41.0 25 35.73608520691874 37.0 34.0 40.0 30.0 41.0 26 35.846445069773246 37.0 34.0 40.0 30.0 41.0 27 35.97411330829884 38.0 35.0 40.0 30.0 41.0 28 35.82153414711447 38.0 35.0 40.0 30.0 41.0 29 35.84350431700969 38.0 35.0 40.0 30.0 41.0 30 35.3241858186952 37.0 34.0 40.0 28.0 41.0 31 35.50300894134821 37.0 34.0 40.0 30.0 41.0 32 35.09452858638341 37.0 34.0 40.0 27.0 41.0 33 35.04447240283127 38.0 34.0 40.0 26.0 41.0 34 34.90094791736696 38.0 34.0 40.0 25.0 41.0 35 34.811617029858915 38.0 34.0 40.0 25.0 41.0 36 34.57646629467072 38.0 34.0 40.0 23.0 41.0 37 34.592758564390195 38.0 34.0 40.0 23.0 41.0 38 34.54545489469186 38.0 34.0 40.0 23.0 41.0 39 34.40156737704446 37.0 34.0 40.0 23.0 41.0 40 34.237987764470866 37.0 33.0 40.0 22.0 41.0 41 34.00838431470473 37.0 33.0 40.0 21.0 41.0 42 34.22723317614744 37.0 34.0 40.0 22.0 41.0 43 34.14981128088895 37.0 33.0 40.0 22.0 41.0 44 34.2700728945574 37.0 34.0 40.0 22.0 41.0 45 34.30910941866831 37.0 34.0 40.0 23.0 41.0 46 33.927264869433266 37.0 33.0 40.0 21.0 41.0 47 33.707951173131775 36.0 33.0 40.0 21.0 41.0 48 33.81743130720398 36.0 33.0 40.0 22.0 41.0 49 33.95588678774142 36.0 34.0 40.0 22.0 41.0 50 33.73677477598609 36.0 33.0 40.0 20.0 41.0 51 31.47051275894855 34.0 29.0 38.0 15.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 13.0 10 21.0 11 17.0 12 19.0 13 18.0 14 25.0 15 49.0 16 126.0 17 250.0 18 632.0 19 1459.0 20 2507.0 21 3462.0 22 4181.0 23 4843.0 24 5622.0 25 7281.0 26 8968.0 27 9521.0 28 9867.0 29 11159.0 30 14564.0 31 19745.0 32 28651.0 33 43842.0 34 58484.0 35 54407.0 36 69262.0 37 82743.0 38 65301.0 39 13572.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.74741411599743 22.203355646687093 24.691950865802944 13.357279371512536 2 29.546978093216676 31.631435897928412 25.515784216743658 13.305801792111252 3 24.71538468926174 31.55940570287064 30.6416449775746 13.083564630293019 4 22.942673568760025 24.15086004052899 38.67426024989676 14.23220614081423 5 27.744494492091082 28.8032423191802 28.737166620247205 14.715096568481508 6 19.328486501541448 40.566829614974594 27.853019985978122 12.251663897505836 7 71.59705348482083 5.44894019573005 19.22860463106134 3.7254016883877723 8 68.4382893308875 11.301249483783602 12.94795578306426 7.312505402264629 9 64.20617923033336 6.320601596189122 14.926577221171113 14.546641952306407 10 42.53892031539621 21.664569787654987 20.505940090085765 15.290569806863036 11 35.62248494568923 22.091564783957434 26.056106719937 16.229843550416334 12 28.820145404953756 19.127186116420003 31.2678274732768 20.784841005349442 13 22.51126072049403 28.77673520739894 34.69108650346225 14.020917568644775 14 15.29786886662889 37.49834330551367 28.121164392113172 19.082623435744264 15 12.247438126062447 23.715221420819606 46.739337130124945 17.298003322993 16 13.787923897697915 23.186231668315358 38.21826109505104 24.807583338935682 17 13.450630504307407 24.91130681981887 32.18020994400853 29.4578527318652 18 16.599982712753185 23.934001133275068 35.44231341778474 24.023702736187012 19 19.149659537278026 27.291376545047687 29.770367738155834 23.788596179518454 20 24.080558570152608 23.896737512365185 33.24740931398442 18.775294603497787 21 19.19249349327238 31.74092179441622 30.811828318431083 18.254756393880314 22 16.83547343046205 24.713848045100505 31.396713502300166 27.053965022137277 23 17.50871565360199 30.921890456479357 28.85971399210549 22.70967989781316 24 18.923772845576867 25.04134533196316 33.06762194712024 22.967259875339742 25 15.232945650816824 32.025393044764364 30.337581514170743 22.404079790248073 26 15.106748749075614 29.67183043131681 32.30045234962496 22.92096846998262 27 20.18689434610989 31.503702352025968 27.878182534118302 20.431220767745838 28 14.792697098623744 29.997598993498077 36.093850542147266 19.115853365730914 29 16.956676238679254 28.251394984777615 32.44643354494204 22.345495231601088 30 18.576875426178656 32.37478751092458 30.60687840342672 18.44145865947005 31 26.688435792284125 28.48630946092602 22.80629639945065 22.018958347339204 32 25.8048653995755 33.26661736599983 23.919979255303822 17.008537979120845 33 22.781902173391085 27.993622926730886 25.248792293729533 23.975682606148496 34 25.670409035467667 28.445204229613054 26.362283069062553 19.522103665856726 35 22.506842868530487 28.65995025114528 24.74054723740192 24.092659642922314 36 25.94335545460657 28.18800841312678 26.067823631666393 19.80081250060025 37 23.281887767352075 31.315655522795154 27.35514727773883 18.04730943211394 38 24.607435436935162 27.18784514468465 25.62872756259424 22.575991855785947 39 24.902279035371627 27.347271976412515 26.50691970073855 21.243529287477312 40 25.434726237238653 23.55464210597082 29.894643834695504 21.115987822095022 41 22.895805921842435 24.00430260365145 24.71115891781835 28.388732556687764 42 25.80813076841812 23.56635901770022 27.576424036956293 23.049086176925368 43 23.759976182015503 23.711763971456833 26.271813144069995 26.25644670245767 44 22.656089432690184 25.875358950472037 27.362062176464374 24.106489440373405 45 19.89608443859666 26.54591204632982 26.640607742765766 26.91739577230775 46 26.718784514468464 27.080087972878232 26.30100938313341 19.900118129519896 47 19.305628919643112 24.48680887027842 34.406231092073796 21.80133111800467 48 23.427676882148994 23.342777292240907 28.415047587948866 24.81449823766123 49 21.813432190774375 19.176934971139904 34.19379003678342 24.815842801302306 50 21.504566714366664 22.101552971005447 31.176012984643165 25.217867329984728 51 19.447192262996648 23.15031261104655 26.387445617202733 31.01504950875407 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 573.0 1 582.5 2 592.0 3 3164.0 4 5736.0 5 4126.5 6 2517.0 7 2668.0 8 2819.0 9 2977.0 10 3135.0 11 3278.5 12 3422.0 13 3426.5 14 3431.0 15 3280.5 16 3130.0 17 3105.0 18 3080.0 19 3181.0 20 3282.0 21 3068.5 22 2855.0 23 3039.0 24 3223.0 25 3205.5 26 3403.0 27 3618.0 28 4368.5 29 5119.0 30 6254.0 31 7389.0 32 8181.0 33 8973.0 34 9452.0 35 9931.0 36 10466.0 37 11001.0 38 12679.5 39 14358.0 40 18448.0 41 22538.0 42 27400.0 43 32262.0 44 38185.5 45 44109.0 46 64847.5 47 85586.0 48 73408.5 49 61231.0 50 56311.0 51 51391.0 52 43943.5 53 36496.0 54 30193.5 55 23891.0 56 21393.5 57 18896.0 58 16345.5 59 13795.0 60 12239.5 61 10684.0 62 8679.0 63 6674.0 64 5706.5 65 4739.0 66 3959.0 67 3179.0 68 2362.0 69 1545.0 70 1363.0 71 1181.0 72 837.0 73 493.0 74 387.5 75 227.0 76 172.0 77 121.0 78 70.0 79 43.5 80 17.0 81 11.5 82 6.0 83 4.0 84 2.0 85 1.0 86 0.0 87 0.5 88 1.0 89 2.0 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 520615.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.79056130821402 #Duplication Level Percentage of deduplicated Percentage of total 1 77.50943170754614 22.31540045540935 2 9.65985873662278 5.562255103708501 3 3.755712029391525 3.243871723145808 4 1.9437167582163282 2.238427859729208 5 1.1502481359038803 1.655814473819978 6 0.7579360233942373 1.3092842129541433 7 0.5321890401049941 1.072541483069168 8 0.3665858398231781 0.8443369676921867 9 0.284266338324898 0.7365768697264036 >10 2.615710279332758 17.580284567429842 >50 1.1435167844227436 23.706713431561088 >100 0.2687062408625882 10.007234870189194 >500 0.004040695351317115 0.7932032211641251 >1k 0.006061043026975673 3.762237913338715 >5k 0.0013468984504390383 3.1830890446960263 >10k+ 6.734492252195192E-4 1.988727802366275 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC 10257 1.9701698952200764 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG 8683 1.667835156497604 No Hit GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC 7734 1.4855507428714116 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3422 0.6572995399671542 No Hit GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC 3215 0.6175388722952662 Illumina Single End Adapter 2 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGATGGCT 2392 0.4594566042084842 No Hit GAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT 2259 0.43390989502799576 No Hit GAATGATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTC 2049 0.3935729857956456 No Hit GAATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT 1800 0.3457449362772874 No Hit CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT 1732 0.3326834609068121 Illumina Single End Adapter 2 (95% over 22bp) GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTA 1498 0.28773661919076476 No Hit CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC 1037 0.19918749939974836 Illumina Single End Adapter 2 (95% over 21bp) TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC 826 0.15865850964724412 Illumina Single End Adapter 2 (95% over 21bp) GAATGATCCGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC 800 0.15366441612323886 No Hit GAATGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT 704 0.1352246861884502 No Hit ACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC 663 0.1273493848621342 Illumina Single End Adapter 2 (95% over 21bp) GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGT 560 0.1075650912862672 No Hit GAATGATCCCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG 538 0.10333931984287813 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.41258895729089634 0.0 2 0.0 0.0 0.0 1.7855805153520357 0.0 3 0.0 0.0 0.0 2.2917126859579535 0.0 4 0.0 0.0 0.0 3.48760600443706 0.0 5 0.0 0.0 0.0 6.815401016105952 0.0 6 0.0 0.0 0.0 8.197036197574024 0.0 7 0.0 0.0 0.0 9.447288303256725 0.0 8 0.0 0.0 0.0 11.896507015741 0.0 9 0.0 0.0 0.0 12.65003889630533 0.0 10 0.0 0.0 0.0 16.237910932262803 0.0 11 0.0 0.0 0.0 18.163133985766834 0.0 12 0.0 0.0 0.0 22.103473776206986 0.0 13 0.0 0.0 0.0 23.07232791986401 0.0 14 0.0 0.0 0.0 23.482227749872745 0.0 15 0.0 0.0 0.0 24.201377217329505 0.0 16 0.0 0.0 0.0 25.06554747750257 0.0 17 0.0 0.0 0.0 25.902442303813757 0.0 18 0.0 0.0 0.0 27.27697050603613 0.0 19 0.0 0.0 0.0 28.41754463471087 0.0 20 0.0 0.0 0.0 29.044879613533993 0.0 21 0.0 0.0 0.0 29.56983567511501 0.0 22 0.0 0.0 0.0 30.0865322743294 0.0 23 0.0 0.0 0.0 30.572111829278835 0.0 24 0.0 0.0 0.0 30.961074882590783 0.0 25 0.0 0.0 0.0 31.287035525292204 0.0 26 0.0 0.0 0.0 31.59628516274022 0.0 27 0.0 0.0 0.0 31.902845672906082 0.0 28 0.0 0.0 0.0 32.164075180315585 0.0 29 0.0 0.0 0.0 32.46756240215898 0.0 30 0.0 0.0 0.0 32.819645995601356 0.0 31 0.0 0.0 0.0 33.13388972657338 0.0 32 0.0 0.0 0.0 33.40222621322859 0.0 33 3.8416104030809713E-4 0.0 0.0 33.684200416814726 0.0 34 3.8416104030809713E-4 0.0 0.0 33.9982520672666 0.0 35 3.8416104030809713E-4 0.0 0.0 34.31211163719831 0.0 36 3.8416104030809713E-4 0.0 0.0 34.589860069341064 0.0 37 3.8416104030809713E-4 0.0 0.0 34.85646783131489 0.0 38 3.8416104030809713E-4 0.0 0.0 35.106556668555456 0.0 39 3.8416104030809713E-4 0.0 0.0 35.3956378513873 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATAAC 20 7.029725E-4 45.000004 43 AGGTACA 20 7.029725E-4 45.000004 15 ATCTCCG 20 7.029725E-4 45.000004 10 ACCGGTA 20 7.029725E-4 45.000004 22 GCGACTT 20 7.029725E-4 45.000004 9 CTACTTA 20 7.029725E-4 45.000004 25 TTGTGCG 40 6.8030204E-9 45.000004 1 TCCGAGT 20 7.029725E-4 45.000004 13 GCAACCG 20 7.029725E-4 45.000004 2 CGTTGAT 20 7.029725E-4 45.000004 25 CGTTGAA 20 7.029725E-4 45.000004 27 TGGAACG 20 7.029725E-4 45.000004 1 TAGCGAA 20 7.029725E-4 45.000004 43 CACGCTT 20 7.029725E-4 45.000004 18 CCAATGT 20 7.029725E-4 45.000004 38 GAATCGT 20 7.029725E-4 45.000004 25 GTGATAA 20 7.029725E-4 45.000004 9 CACGATC 20 7.029725E-4 45.000004 45 TAACGTG 20 7.029725E-4 45.000004 1 TCGATTG 40 6.8030204E-9 45.000004 1 >>END_MODULE