##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	582328
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.639165556181396	28.0	25.0	31.0	16.0	33.0
2	27.416574507837506	30.0	25.0	31.0	16.0	33.0
3	27.36489057713179	30.0	25.0	31.0	16.0	33.0
4	30.39156626506024	33.0	28.0	35.0	19.0	37.0
5	31.797663859543075	33.0	32.0	35.0	26.0	37.0
6	31.015408841752414	33.0	30.0	35.0	17.0	37.0
7	31.85228084515943	33.0	30.0	35.0	26.0	35.0
8	33.17921343297935	35.0	33.0	35.0	28.0	37.0
9	34.32840598425629	37.0	33.0	38.0	28.0	39.0
10	33.77445700704757	35.0	32.0	38.0	27.0	39.0
11	33.83674836174802	37.0	32.0	39.0	26.0	39.0
12	33.137369317635425	35.0	31.0	37.0	23.0	39.0
13	33.31845969968815	35.0	32.0	38.0	23.0	39.0
14	33.9207869104697	36.0	32.0	39.0	22.0	40.0
15	35.139500419007845	37.0	33.0	39.0	27.0	41.0
16	35.157042079377945	36.0	33.0	39.0	27.0	41.0
17	34.877915882458	36.0	33.0	39.0	27.0	40.0
18	34.5785244741795	36.0	33.0	39.0	27.0	40.0
19	33.90601688395543	36.0	32.0	38.0	25.0	40.0
20	34.07018037944251	35.0	32.0	38.0	27.0	40.0
21	34.29514466074102	35.0	33.0	39.0	27.0	40.0
22	34.654795922572845	35.0	33.0	39.0	27.0	40.0
23	35.07517069417923	35.0	33.0	39.0	29.0	40.0
24	34.746984517316704	35.0	33.0	39.0	27.0	40.0
25	33.920233957494744	35.0	33.0	39.0	24.0	40.0
26	33.89291430259235	35.0	32.0	39.0	24.0	40.0
27	34.39481701034469	35.0	33.0	39.0	26.0	40.0
28	34.294505845502876	35.0	33.0	39.0	25.0	40.0
29	34.45991949554203	36.0	33.0	39.0	25.0	40.0
30	33.911086191974285	35.0	33.0	39.0	23.0	40.0
31	33.79606338695718	35.0	33.0	39.0	23.0	40.0
32	33.23888083691665	35.0	32.0	39.0	21.0	40.0
33	33.17153734665	35.0	32.0	39.0	19.0	40.0
34	32.880855806349686	35.0	32.0	39.0	16.0	40.0
35	32.803296424008465	35.0	32.0	39.0	15.0	40.0
36	32.53099627701227	35.0	32.0	39.0	14.0	40.0
37	32.52926529378632	35.0	32.0	39.0	12.0	40.0
38	32.442777953318405	35.0	31.0	39.0	12.0	40.0
39	32.27148445549587	35.0	31.0	39.0	11.0	40.0
40	32.10596605349563	35.0	31.0	39.0	10.0	40.0
41	31.899099820032696	35.0	31.0	39.0	10.0	40.0
42	31.94580545671855	35.0	31.0	39.0	10.0	40.0
43	31.942619966754133	35.0	31.0	39.0	10.0	40.0
44	31.912259070489483	35.0	31.0	39.0	10.0	40.0
45	31.94359536206399	35.0	31.0	39.0	10.0	40.0
46	31.675043961478753	35.0	31.0	39.0	10.0	40.0
47	31.77783826297207	35.0	31.0	39.0	10.0	40.0
48	31.5994851698699	35.0	31.0	38.0	10.0	40.0
49	31.698778008270253	35.0	31.0	38.0	10.0	40.0
50	31.560201467214352	35.0	31.0	39.0	10.0	40.0
51	29.547287782830296	34.0	26.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	18.0
9	29.0
10	28.0
11	38.0
12	26.0
13	36.0
14	65.0
15	156.0
16	477.0
17	988.0
18	1926.0
19	3094.0
20	4695.0
21	6385.0
22	8425.0
23	10804.0
24	13288.0
25	15796.0
26	17272.0
27	17480.0
28	18062.0
29	20962.0
30	26093.0
31	33221.0
32	43282.0
33	54237.0
34	60989.0
35	56563.0
36	63743.0
37	63501.0
38	36874.0
39	3773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.36744927257491	20.943179788710143	24.147044277451883	21.54232666126307
2	33.56579110054814	27.783482848154307	26.65319201549642	11.997534035801129
3	25.233201906829137	26.817017213666528	35.99449107719361	11.955289802310725
4	22.343765025896058	24.624953634377878	39.6122116745202	13.419069665205862
5	23.66741767526205	28.437581569150034	33.194179225453695	14.70082153013422
6	19.303382286271653	37.70761495239796	32.19251006305724	10.796492698273138
7	70.24683683422401	5.461011663529832	21.75406300229424	2.5380884999519173
8	68.36988776084955	9.593390666428542	17.15510846121086	4.881613111511038
9	64.43602231045047	6.2847742165927105	18.955124946765398	10.324078526191425
10	41.9940308554629	21.938323419104012	23.94475278537182	12.122892940061272
11	36.3983871632482	21.062700059073237	29.173249440178044	13.365663337500516
12	31.347281944196396	18.928507645175916	33.35611545383358	16.36809495679411
13	19.398517673888254	31.85730378755615	34.899403772444394	13.844774766111195
14	13.97082056847687	37.28190985149263	32.75250374359467	15.994765836435823
15	11.230612300971273	21.8528046049649	52.946449423692485	13.970133670371338
16	12.244473904740971	19.933954747152807	46.731567089337965	21.090004258768253
17	13.347975711282988	19.268178758362982	36.61561868912366	30.768226841230373
18	16.965352859556813	22.11983624349164	39.66166833811872	21.25314255883282
19	20.80133532991716	24.904177714277864	33.719999725240754	20.574487230564216
20	25.099256776249813	21.194756219862345	36.14990177357091	17.556085230316935
21	17.55814592463354	28.39001387534173	35.60605019851355	18.445790001511174
22	17.143087744364003	23.22797461224602	33.62555123572969	26.00338640766029
23	16.179541426824745	30.47354755395585	32.59571925100631	20.751191768213104
24	17.00450605157231	22.46551771510214	39.811068676072594	20.718907557252958
25	14.603453723674631	27.35039359261447	37.049394842769026	20.996757840941875
26	13.481405668283166	29.362833317305707	35.945549587174234	21.21021142723688
27	16.86128779656826	30.65437348023794	33.67122995974777	18.813108763446028
28	12.15981371323378	27.0625832863953	43.47069005783682	17.306912942534105
29	14.855201879353217	21.64398758088225	41.43729994092676	22.06351059883777
30	16.59666030141089	24.995535162314024	38.89835281834293	19.509451717932162
31	23.450529598439367	24.679046860188762	31.677679932958746	20.192743608413128
32	22.75624733826984	26.218213790166367	31.830514761440288	19.195024110123505
33	20.818507782555535	24.722664889890233	30.044751411575604	24.41407591597862
34	19.531432457309283	24.032332293827533	34.57776373452762	21.858471514335566
35	16.67496668544188	25.464343119341677	33.2809001112775	24.57979008393895
36	23.3634652635628	23.197235922023328	34.085601241911775	19.353697572502096
37	19.75810883213584	25.337438694344083	35.86947562198623	19.034976851533845
38	18.732913409624814	28.13070984050226	29.18784602492066	23.94853072495226
39	22.003922188182607	25.217059801349066	29.261172397686526	23.517845612781798
40	21.9881235317553	24.413045568820323	30.06312593589867	23.53570496352571
41	17.87446250223242	25.216372903243535	29.66730090258411	27.241863691939937
42	21.218969378082456	26.13406877223833	30.577097443365254	22.069864406313968
43	21.70838427827616	25.413340935005703	30.093864626121363	22.784410160596778
44	19.105383907351182	26.817017213666528	31.37407097031226	22.70352790867003
45	17.48361748018299	27.548048522482176	28.639014438598174	26.329319558736657
46	23.66638732810375	27.390061958209117	28.445824346416455	20.49772636727068
47	19.669155527469055	24.02975642593178	34.132482037614544	22.168606008984625
48	20.862297536783394	24.17812641672734	31.273612122377763	23.685963924111498
49	19.692510063057245	21.439807119011967	35.70015523897185	23.16752757895894
50	18.80125977112555	26.013002981137777	31.368575785467982	23.817161462268686
51	17.20250443049278	25.42896786690662	27.904548639254852	29.46397906334574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	180.0
2	294.0
3	4414.5
4	8535.0
5	7445.0
6	6355.0
7	6831.0
8	7307.0
9	7693.0
10	8079.0
11	8151.0
12	8223.0
13	7922.0
14	7621.0
15	7520.0
16	7419.0
17	6982.5
18	6546.0
19	6124.0
20	5702.0
21	5437.0
22	5172.0
23	4913.5
24	4655.0
25	4514.0
26	4360.5
27	4348.0
28	4885.5
29	5423.0
30	6269.5
31	7116.0
32	8354.0
33	9592.0
34	10141.5
35	10691.0
36	11742.0
37	12793.0
38	14638.5
39	16484.0
40	23345.5
41	30207.0
42	39472.5
43	48738.0
44	70527.5
45	92317.0
46	77594.0
47	62871.0
48	56554.0
49	50237.0
50	43129.0
51	36021.0
52	31659.5
53	27298.0
54	24279.0
55	21260.0
56	19410.0
57	17560.0
58	16762.0
59	15964.0
60	14248.5
61	12533.0
62	10504.5
63	8476.0
64	6718.0
65	4960.0
66	4154.0
67	3348.0
68	2390.5
69	1433.0
70	1192.0
71	951.0
72	776.5
73	602.0
74	475.0
75	278.5
76	209.0
77	156.5
78	104.0
79	80.0
80	56.0
81	38.0
82	20.0
83	16.0
84	12.0
85	9.5
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	582328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.51252476884152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.74290315335277	26.901806882192368
2	8.126627648637614	5.284343654269655
3	2.8741643346922374	2.8033901736420686
4	1.4246865250433827	1.8528062373323095
5	0.7859178926423661	1.2776087475405329
6	0.5350872196298406	1.043822188902344
7	0.3692888452178196	0.8404558908900871
8	0.25320252899925494	0.6585802796497273
9	0.2120443140646305	0.6204686411806479
>10	1.4899377695653466	10.99427969507467
>50	0.655738946657912	15.678657941624923
>100	0.5223318434822251	23.97638731474199
>500	0.002151727470575593	0.4814865728899606
>1k	0.004303454941151186	2.9810892244494656
>5k	0.0010758637352877964	2.7843320887788496
>10k+	5.379318676438982E-4	1.8204844668403923
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	10409	1.7874805951285186	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	7960	1.3669272300146995	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	7960	1.3669272300146995	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3700	0.635380747619898	No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	3109	0.5338915525270982	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2634	0.4523224024948139	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC	2041	0.35048975834924645	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT	1568	0.26926405736972975	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	1492	0.25621299336456427	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTA	1352	0.23217155967083844	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCCGAAT	1149	0.19731148081493594	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	899	0.15438034921899685	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	740	0.1270761495239796	No Hit
TGTTAGGGGAAACTATCAATCATCAAAAGCTTACTATTCACCTACAGCATT	589	0.10114574604003243	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29811377780220083	0.0
2	0.0	0.0	0.0	1.17631300572873	0.0
3	0.0	0.0	0.0	1.414494923823	0.0
4	0.0	0.0	0.0	2.405001992004506	0.0
5	0.0	0.0	0.0	4.819448832960119	0.0
6	0.0	0.0	0.0	5.547560824827245	0.0
7	0.0	0.0	0.0	6.354150925251748	0.0
8	0.0	0.0	0.0	8.222513772307016	0.0
9	0.0	0.0	0.0	8.777870890632084	0.0
10	0.0	0.0	0.0	11.314413869846547	0.0
11	0.0	0.0	0.0	12.591357448036158	0.0
12	0.0	0.0	0.0	15.712278990534545	0.0
13	0.0	0.0	0.0	16.564032641397976	0.0
14	0.0	0.0	0.0	16.80152766138671	0.0
15	0.0	0.0	0.0	17.295063950213624	0.0
16	0.0	0.0	0.0	17.944354384470607	0.0
17	0.0	0.0	0.0	18.51190394416892	0.0
18	0.0010303471583025373	0.0	0.0	19.74660328886813	0.0
19	0.0010303471583025373	0.0	0.0	20.30985973540685	0.0
20	0.0010303471583025373	0.0	0.0	20.87723757057878	0.0
21	0.0010303471583025373	0.0	0.0	21.30156887527304	0.0
22	0.0010303471583025373	0.0	0.0	21.645533101619705	0.0
23	0.0010303471583025373	0.0	0.0	21.975415916802902	0.0
24	0.0010303471583025373	0.0	0.0	22.23008338943001	0.0
25	0.0010303471583025373	0.0	0.0	22.450405956780372	0.0
26	0.0010303471583025373	0.0	0.0	22.654929867703427	0.0
27	0.0010303471583025373	0.0	0.0	22.874222087895483	0.0
28	0.0010303471583025373	0.0	0.0	23.043542470909866	0.0
29	0.0010303471583025373	0.0	0.0	23.230722204668158	0.0
30	0.0010303471583025373	0.0	0.0	23.49397590361446	0.0
31	0.0010303471583025373	0.0	0.0	23.71773296149249	0.0
32	0.0010303471583025373	0.0	0.0	23.939601049580304	0.0
33	0.0010303471583025373	0.0	0.0	24.14275116429229	0.0
34	0.0010303471583025373	0.0	0.0	24.354315780797076	0.0
35	0.0010303471583025373	0.0	0.0	24.58356802351939	0.0
36	0.0010303471583025373	0.0	0.0	24.774010523278978	0.0
37	0.00137379621107005	0.0	0.0	24.9598164608262	0.0
38	0.00137379621107005	0.0	0.0	25.13583410036955	0.0
39	0.00137379621107005	0.0	0.0	25.341388358450907	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	40	6.8048394E-9	45.000004	1
CTGCTAT	20	7.0304144E-4	45.000004	36
CGGAACG	20	7.0304144E-4	45.000004	33
ATCTCGA	20	7.0304144E-4	45.000004	22
AGTCCGA	20	7.0304144E-4	45.000004	10
AGTCCAT	20	7.0304144E-4	45.000004	28
ATTAGTA	20	7.0304144E-4	45.000004	12
GTATTAC	20	7.0304144E-4	45.000004	20
AGTCAGG	65	0.0	45.000004	2
CAAGTGC	30	2.1636497E-6	45.000004	35
ATCTACG	20	7.0304144E-4	45.000004	13
GTACACT	30	2.1636497E-6	45.000004	18
TACCACG	20	7.0304144E-4	45.000004	29
ACGTTAG	30	2.1636497E-6	45.000004	1
GCGGTAT	30	2.1636497E-6	45.000004	22
TTAGCCA	20	7.0304144E-4	45.000004	30
CCTAGAT	20	7.0304144E-4	45.000004	35
GCTGGAA	30	2.1636497E-6	45.000004	25
TAGACGA	30	2.1636497E-6	45.000004	34
GAGTCCG	20	7.0304144E-4	45.000004	9
>>END_MODULE