##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548204_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	436290
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.427722386486053	28.0	25.0	31.0	16.0	33.0
2	27.141719956909395	30.0	25.0	31.0	16.0	33.0
3	27.140518920901236	30.0	25.0	31.0	16.0	33.0
4	29.989667423044306	33.0	28.0	35.0	19.0	37.0
5	31.57232345458296	33.0	30.0	35.0	25.0	37.0
6	30.74259552132756	33.0	28.0	35.0	17.0	36.0
7	31.781223498131975	33.0	30.0	35.0	25.0	35.0
8	33.16012056201151	35.0	33.0	35.0	28.0	37.0
9	34.244651493272826	37.0	32.0	38.0	27.0	39.0
10	33.83280157693277	35.0	32.0	39.0	27.0	39.0
11	34.00071512067662	37.0	32.0	39.0	26.0	39.0
12	33.45986614407848	35.0	31.0	38.0	25.0	39.0
13	33.66918104930207	35.0	32.0	39.0	25.0	39.0
14	34.24949460221412	37.0	32.0	39.0	24.0	40.0
15	35.430807490430674	37.0	33.0	40.0	27.0	41.0
16	35.38670838203947	37.0	33.0	40.0	27.0	41.0
17	35.034440395150014	36.0	33.0	39.0	27.0	40.0
18	34.72550367874579	36.0	33.0	39.0	27.0	40.0
19	34.207854867175506	36.0	32.0	39.0	26.0	40.0
20	34.315400307135164	35.0	32.0	38.0	27.0	40.0
21	34.219537463613655	35.0	33.0	39.0	27.0	40.0
22	34.56273808705219	35.0	33.0	39.0	27.0	40.0
23	35.01048843659034	36.0	33.0	39.0	29.0	40.0
24	34.60020857686401	35.0	33.0	39.0	27.0	40.0
25	33.653191684430084	35.0	32.0	39.0	23.0	40.0
26	33.70804969171881	35.0	32.0	39.0	24.0	40.0
27	34.262004629947974	35.0	33.0	39.0	25.0	40.0
28	34.23966169291068	35.0	33.0	39.0	25.0	40.0
29	34.28390061656238	36.0	33.0	39.0	25.0	40.0
30	33.61660134314332	35.0	33.0	39.0	22.0	40.0
31	33.49555800041257	35.0	33.0	39.0	22.0	40.0
32	32.920250292236815	35.0	32.0	39.0	18.0	40.0
33	32.78990350454973	35.0	32.0	39.0	16.0	40.0
34	32.42958582594146	35.0	31.0	39.0	15.0	40.0
35	32.30140732081872	36.0	31.0	39.0	10.0	40.0
36	31.949203511425885	35.0	31.0	39.0	10.0	40.0
37	31.970359164775722	35.0	31.0	39.0	10.0	40.0
38	31.900288798734785	35.0	30.0	39.0	10.0	40.0
39	31.65588484723464	35.0	30.0	39.0	10.0	40.0
40	31.552735565793395	35.0	30.0	39.0	10.0	40.0
41	31.394235485571524	35.0	30.0	39.0	10.0	40.0
42	31.42190744688166	35.0	30.0	39.0	9.0	40.0
43	31.37072360127438	35.0	30.0	39.0	9.0	40.0
44	31.242962249879668	35.0	30.0	39.0	8.0	40.0
45	31.236814962524925	35.0	29.0	39.0	9.0	40.0
46	30.947690756148432	35.0	29.0	39.0	8.0	40.0
47	31.0793027573403	35.0	29.0	39.0	8.0	40.0
48	30.91906988470971	35.0	29.0	39.0	8.0	40.0
49	30.99121685117697	35.0	29.0	38.0	8.0	40.0
50	30.943984505718674	35.0	29.0	39.0	8.0	40.0
51	29.147092530197803	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	29.0
10	48.0
11	28.0
12	24.0
13	34.0
14	71.0
15	200.0
16	475.0
17	967.0
18	1831.0
19	2937.0
20	4161.0
21	5627.0
22	7392.0
23	9164.0
24	11481.0
25	13460.0
26	14335.0
27	14269.0
28	14073.0
29	15718.0
30	18877.0
31	23669.0
32	29520.0
33	37292.0
34	42200.0
35	42127.0
36	47876.0
37	47819.0
38	27844.0
39	2728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.394874968484263	22.562057347177337	24.476838799880813	24.566228884457587
2	37.20323637947237	24.239382062389694	25.4938229159504	13.063558642187537
3	26.369845744802767	22.128171628962388	38.313048660294754	13.188933965940086
4	22.431639505833274	26.630223016800752	36.309793944394784	14.628343532971188
5	20.739187237846387	29.775149556487655	35.90960141190492	13.57606179376103
6	18.128767562859565	35.38678401980334	35.02693162804557	11.457516789291526
7	71.08597492493524	6.472300534048454	19.91565243301474	2.5260721080015585
8	69.27410667216759	6.812212060785258	19.243851566618535	4.669829700428614
9	64.15640972747485	7.233720690366499	21.272777281166196	7.33709230099246
10	35.794081917990326	25.089046276559166	27.11957642852231	11.99729537692819
11	30.77379724495175	23.076623346856447	32.40986499805176	13.739714410140044
12	28.049004102775676	20.551926470925302	36.48994934561874	14.909120080680282
13	20.214536202984252	28.54500446950423	37.63483004423664	13.605629283274887
14	14.04203626028559	34.239382062389694	35.4433977400353	16.275183937289416
15	11.086204130280318	23.62579935364093	51.10774943271677	14.180247083361985
16	11.885901579224829	23.913910472392217	46.54747988723097	17.652708061151987
17	13.791973228815696	21.441014004446586	37.46773934768159	27.29927341905613
18	16.46427834697105	23.795869719681864	40.353205436750784	19.3866464965963
19	21.42840771046781	25.533933851337416	35.55983405533017	17.47782438286461
20	23.937747828279356	23.402324142198996	35.627220426780355	17.032707602741297
21	17.58142519883564	28.26674918059089	37.90002062848106	16.251804992092417
22	17.90758440486832	23.793348460886108	34.831877879392145	23.467189254853423
23	15.577712072245525	28.999518668775355	35.53072497650645	19.892044282472668
24	16.694171308074903	23.180224162827475	40.3933163721378	19.73228815695982
25	14.549726099612641	26.11565701712164	38.70338536294666	20.631231520319055
26	14.564166036351967	28.270874876802125	37.349469389626165	19.81548969721974
27	17.608700634898806	29.875083086937586	34.757844552934976	17.758371725228635
28	13.773636801210204	25.891494189644504	42.22764674872218	18.107222260423114
29	15.044580439615851	25.323523344564396	39.02656489949345	20.605331316326296
30	17.487221802012424	26.846363657200484	36.11038529418506	19.55602924660203
31	20.922322308556236	27.566526851406177	31.203098856265328	20.308051983772263
32	22.639757959155606	26.885557771207225	31.12173095876596	19.35295331087121
33	21.524903160741708	27.92317036833299	29.503312017236244	21.04861445368906
34	16.7709550986729	27.190859290838667	32.97966948589241	23.058516124596025
35	17.88260102225584	26.759953242109606	32.433473148593826	22.92397258704073
36	21.51115084003759	26.913750028650668	31.525361571431848	20.049737559879897
37	19.426986637328383	29.248435673519907	33.01313346627243	18.311444222879278
38	18.18767333654221	31.442847647207135	29.476494991863213	20.89298402438745
39	19.072405968507187	28.10080451076119	32.714249696302915	20.112539824428705
40	21.669531733480024	27.31073368630956	31.896445025097986	19.123289555112425
41	17.20415320085264	26.944692750234932	30.653464438790714	25.19768961012171
42	21.505191501065806	25.94787870453139	30.07105365697128	22.475876137431523
43	21.679387563317974	24.483714960232874	32.3912993651012	21.44559811134796
44	19.992665428957803	26.721217538793006	29.498040294299667	23.788076737949527
45	17.779687822320017	28.585344610236312	30.37589676591258	23.25907080153109
46	21.16871805450503	29.665589401544846	29.63418826927044	19.531504274679687
47	18.10263815352174	27.52274863049806	33.1595956817713	21.215017534208897
48	19.733204978340094	24.885970340828347	32.86185793852713	22.518966742304432
49	20.00320887483096	22.488253226065233	35.63455499782255	21.87398290128126
50	17.625661830433888	25.861009878750373	34.18070549405212	22.33262279676362
51	16.04437415480529	25.943065392284947	30.253042700955785	27.75951775195398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82.0
1	174.5
2	267.0
3	4702.5
4	9138.0
5	7630.0
6	6122.0
7	6362.5
8	6603.0
9	7021.0
10	7439.0
11	7373.5
12	7308.0
13	6962.0
14	6616.0
15	6435.5
16	6255.0
17	6082.0
18	5909.0
19	5641.0
20	5373.0
21	5077.0
22	4781.0
23	4926.0
24	5071.0
25	4897.5
26	4589.0
27	4454.0
28	5304.0
29	6154.0
30	7042.0
31	7930.0
32	7839.0
33	7748.0
34	8801.5
35	9855.0
36	10330.5
37	10806.0
38	12499.5
39	14193.0
40	18169.0
41	22145.0
42	28255.5
43	34366.0
44	42401.0
45	50436.0
46	48041.0
47	45646.0
48	40949.0
49	36252.0
50	31719.5
51	27187.0
52	23491.0
53	19795.0
54	17850.0
55	15905.0
56	14575.5
57	13246.0
58	12106.0
59	10966.0
60	9629.5
61	8293.0
62	7323.5
63	6354.0
64	5013.0
65	3672.0
66	2733.0
67	1794.0
68	1649.0
69	1504.0
70	1191.0
71	878.0
72	635.5
73	393.0
74	357.5
75	230.5
76	139.0
77	117.0
78	95.0
79	63.5
80	32.0
81	21.5
82	11.0
83	9.5
84	8.0
85	11.5
86	15.0
87	11.0
88	7.0
89	3.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	436290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.33340575851489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.19415404122468	32.72309417639882
2	7.797029361817589	6.133674391988513
3	2.61678812544738	3.0878156736685614
4	1.2666427745869047	1.9928549681567134
5	0.7706077687586201	1.5155314024623312
6	0.48411737791827286	1.1425191156244634
7	0.3625702456489328	0.9982785811651724
8	0.26796890678968377	0.843210379313943
9	0.2025127632182089	0.7168965018245875
>10	2.1757577157974257	21.097133407411075
>50	0.7323070950342135	19.744790493948265
>100	0.12303705635469822	6.417179060527994
>500	0.002957621546987938	0.8234072983288703
>1k	0.003549145856385526	2.7636145491806503
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3779	0.866166999014417	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	2130	0.48820738499621813	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	1892	0.4336565128698801	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	1876	0.4299892273487818	No Hit
CAGAGCGTCTTTTAAAAAGTTGCAATGGTTTGCTTTATAGAAACAATCTTT	1155	0.2647321735542873	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1046	0.23974879094180476	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	975	0.22347521144193083	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	751	0.17213321414655391	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	685	0.1570056613720232	No Hit
GAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	627	0.14371175135804165	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	501	0.11483187787939214	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27275436063168995	0.0
2	0.0	0.0	0.0	0.9768731806825736	0.0
3	0.0	0.0	0.0	1.2537532375254992	0.0
4	0.0	0.0	0.0	1.9347223177244492	0.0
5	0.0	0.0	0.0	3.5591005982259505	0.0
6	0.0	0.0	0.0	4.449104953127507	0.0
7	0.0	0.0	0.0	5.212817162896239	0.0
8	0.0	0.0	0.0	7.112929473515322	0.0
9	0.0	0.0	0.0	7.798024249925509	0.0
10	0.0	0.0	0.0	9.589722432327122	0.0
11	0.0	0.0	0.0	11.113708771688556	0.0
12	0.0	0.0	0.0	13.092209310321117	0.0
13	0.0	0.0	0.0	13.66591028902794	0.0
14	0.0	0.0	0.0	13.83185495885764	0.0
15	0.0	0.0	0.0	14.16741158403814	0.0
16	0.0	0.0	0.0	14.857548878039836	0.0
17	0.0	0.0	0.0	15.569002269132916	0.0
18	0.001375232070411882	0.0	0.0	17.214238236035666	0.0
19	0.001375232070411882	0.0	0.0	17.72513695019368	0.0
20	0.001375232070411882	0.0	0.0	18.398542254005363	0.0
21	0.001375232070411882	0.0	0.0	18.883082353480482	0.0
22	0.001375232070411882	0.0	0.0	19.271127002681702	0.0
23	0.001375232070411882	0.0	0.0	19.66627701758005	0.0
24	0.001375232070411882	0.0	0.0	19.99610350913383	0.0
25	0.001375232070411882	0.0	0.0	20.246166540603728	0.0
26	0.001375232070411882	0.0	0.0	20.47583029636251	0.0
27	0.001375232070411882	0.0	0.0	20.719704783515553	0.0
28	0.001375232070411882	0.0	0.0	20.93676224529556	0.0
29	0.001375232070411882	0.0	0.0	21.171697723990924	0.0
30	0.001375232070411882	0.0	0.0	21.453161887735224	0.0
31	0.0016044374154805291	0.0	0.0	21.74700314011323	0.0
32	0.0016044374154805291	0.0	0.0	22.003483921245042	0.0
33	0.002062848105617823	0.0	0.0	22.22902198079259	0.0
34	0.002062848105617823	0.0	0.0	22.467624745009054	0.0
35	0.002062848105617823	0.0	0.0	22.738087052190057	0.0
36	0.002062848105617823	0.0	0.0	22.972564120195283	0.0
37	0.002062848105617823	0.0	0.0	23.204519929404753	0.0
38	0.002062848105617823	0.0	0.0	23.455041371564786	0.0
39	0.002062848105617823	0.0	0.0	23.695936189231933	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAGCG	45	3.8380676E-10	45.000004	1
ATAGCGG	45	3.8380676E-10	45.000004	2
CGTCTGG	45	3.8380676E-10	45.000004	2
CGTTAGG	45	3.8380676E-10	45.000004	2
TTACGGG	90	0.0	45.000004	3
TCACGAC	35	1.2096098E-7	45.0	25
CTTAACG	20	7.02847E-4	45.0	1
ATCCTCC	25	3.8868126E-5	45.0	29
TGTCACA	20	7.02847E-4	45.0	42
CGGGTAC	35	1.2096098E-7	45.0	6
CTATGCG	20	7.02847E-4	45.0	1
AACCGCT	25	3.8868126E-5	45.0	15
AGCCGGA	20	7.02847E-4	45.0	21
TTGGCCG	20	7.02847E-4	45.0	36
ACGCCTT	20	7.02847E-4	45.0	17
TCACCTA	20	7.02847E-4	45.0	36
GCGACGA	25	3.8868126E-5	45.0	15
ACGCCAG	20	7.02847E-4	45.0	38
GTCGATA	25	3.8868126E-5	45.0	35
ATGATCG	25	3.8868126E-5	45.0	27
>>END_MODULE