FastQCFastQC Report
Sat 18 Jun 2016
SRR3548203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422730
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40220.9514347219265253No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT24020.568211387883519No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC18700.44236273744470467No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC15160.3586213422278996No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC12250.2897830766683226No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC12110.28647127007782747No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC11580.2739337165566674No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG11480.27156814042059946No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC11040.26115960542190053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9380.22189104156317271No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG6670.15778392827573157No Hit
AAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC5630.13318193646062498No Hit
CAGAGCGTCTTTTAAAAAGTTGCAATGGTTTGCTTTATAGAAACAATCTTT5390.12750455373406194No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG5250.12419274714356682No Hit
GAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT5160.12206372862110568No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4530.10716059896387765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG1250.045.0000045
GCGACGG351.2094824E-745.0000042
TACATCA453.8380676E-1045.00000424
GTGCTAG453.8380676E-1045.0000041
AGTTGCA700.045.00000418
GCGTCTT700.045.0000045
TCACGAC207.028221E-445.025
GTCATCC207.028221E-445.010
CTATGCG207.028221E-445.01
ACCGGGA406.7975634E-945.04
CTCGTAG207.028221E-445.01
GTCTGGC253.886607E-545.014
CGACGGT207.028221E-445.028
CGTGCAA207.028221E-445.034
CAGCCCG207.028221E-445.014
AGCCCAT207.028221E-445.027
GAACTTC207.028221E-445.08
TTAGAGC207.028221E-445.02
GGCAACT253.886607E-545.08
GGCAACG406.7975634E-945.08