##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548203_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	422730
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.160483050646985	28.0	25.0	31.0	16.0	33.0
2	27.38458827147352	30.0	25.0	33.0	16.0	33.0
3	27.294632507747263	30.0	25.0	33.0	16.0	34.0
4	31.616088283301398	33.0	28.0	35.0	19.0	37.0
5	32.95437749863979	35.0	32.0	35.0	28.0	37.0
6	31.59941097154212	35.0	31.0	35.0	19.0	37.0
7	33.629210134128165	35.0	33.0	35.0	30.0	37.0
8	33.49492110803586	35.0	35.0	37.0	28.0	37.0
9	35.966479786151915	38.0	35.0	39.0	31.0	39.0
10	35.296349916022045	37.0	34.0	39.0	30.0	39.0
11	35.73254796205616	37.0	35.0	39.0	31.0	39.0
12	35.720353417074726	37.0	35.0	39.0	31.0	39.0
13	35.77553521160078	37.0	35.0	39.0	31.0	39.0
14	36.326979395831856	38.0	35.0	40.0	31.0	41.0
15	36.86495162396802	38.0	35.0	40.0	32.0	41.0
16	36.36949589572541	38.0	34.0	40.0	31.0	41.0
17	36.18339602110094	38.0	34.0	40.0	31.0	41.0
18	36.10111182078395	38.0	34.0	39.0	31.0	40.0
19	35.69480756038133	37.0	34.0	39.0	30.0	40.0
20	36.03409031769687	37.0	34.0	39.0	31.0	40.0
21	35.91436614387434	37.0	34.0	39.0	30.0	40.0
22	36.44397842594564	38.0	35.0	40.0	31.0	41.0
23	36.58443924017695	38.0	35.0	40.0	32.0	41.0
24	36.60241525323492	38.0	35.0	40.0	32.0	41.0
25	35.75606652000095	38.0	34.0	40.0	29.0	41.0
26	36.06376410474771	38.0	34.0	40.0	30.0	41.0
27	36.32417855368675	38.0	35.0	40.0	31.0	41.0
28	36.24884678163367	38.0	35.0	40.0	31.0	41.0
29	36.29999053769546	38.0	35.0	40.0	31.0	41.0
30	35.61459323918341	38.0	34.0	40.0	29.0	41.0
31	35.505225557684575	38.0	34.0	40.0	29.0	41.0
32	35.212875830908615	38.0	34.0	40.0	27.0	41.0
33	35.01322593617675	38.0	34.0	40.0	25.0	41.0
34	34.81426678967662	38.0	34.0	40.0	24.0	41.0
35	34.68913017765477	38.0	34.0	40.0	23.0	41.0
36	34.302081706999736	38.0	34.0	40.0	21.0	41.0
37	34.39362950346557	38.0	34.0	40.0	21.0	41.0
38	34.23888534052468	38.0	33.0	40.0	21.0	41.0
39	34.00928252075793	38.0	33.0	40.0	19.0	41.0
40	33.91527215953445	37.0	33.0	40.0	19.0	41.0
41	33.67017718165259	37.0	33.0	40.0	18.0	41.0
42	33.905424266080004	37.0	33.0	40.0	18.0	41.0
43	33.942043384666334	37.0	33.0	40.0	18.0	41.0
44	33.9623991673172	37.0	33.0	40.0	18.0	41.0
45	33.94719087833842	37.0	33.0	40.0	20.0	41.0
46	33.54529605185343	37.0	33.0	40.0	18.0	41.0
47	33.55584652142029	37.0	33.0	40.0	18.0	41.0
48	33.59881248077969	37.0	33.0	40.0	18.0	41.0
49	33.73845480566792	37.0	33.0	40.0	18.0	41.0
50	33.634222789960496	37.0	33.0	40.0	18.0	41.0
51	31.70171267712251	35.0	30.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	1.0
11	12.0
12	17.0
13	16.0
14	30.0
15	47.0
16	124.0
17	289.0
18	582.0
19	1238.0
20	2084.0
21	3203.0
22	3959.0
23	4652.0
24	5260.0
25	6556.0
26	7716.0
27	8079.0
28	7543.0
29	8231.0
30	10040.0
31	13825.0
32	20291.0
33	30101.0
34	41126.0
35	49873.0
36	68310.0
37	74857.0
38	47132.0
39	7527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.0690038558891	26.776665956993828	26.22761573581246	15.926714451304615
2	33.82868497622596	26.23707804035673	25.206396517871926	14.727840465545384
3	26.91457904572659	25.205686845031106	33.075958649729145	14.803775459513163
4	23.50247202706219	28.545170676318214	32.35422137061481	15.598135926004778
5	20.866510538641688	31.03943415418825	31.932912260781116	16.161143046388947
6	19.010952617509993	36.394861968632455	30.737113523998772	13.857071889858775
7	71.68996759160694	7.582381188938565	16.04735883424408	4.680292385210418
8	66.9602346651527	7.071889858775105	15.995316159250587	9.972559316821613
9	60.361933148818395	8.966479786151918	21.00063870555674	9.67094835947295
10	32.899723227592084	26.931611193906274	24.23887587822014	15.929789700281505
11	24.808979727012513	25.504222553402883	31.46050670640835	18.22629101317626
12	23.35580630662598	19.256026305206632	37.16059896387765	20.227568424289736
13	21.622548671729	25.549878172828993	38.38715019042888	14.440422965013127
14	14.236510302084074	33.2798713126582	32.68161710784662	19.80200127741111
15	9.991247368296548	25.054763087549976	47.591133820642014	17.362855723511462
16	9.792775530480448	28.03799115274525	40.18285903531805	21.986374281456246
17	11.460506706408346	26.27232512478414	33.160882833013986	29.10628533579353
18	14.491282851938589	25.074633927092943	37.289286305679745	23.14479691528872
19	17.48468289451896	29.840560168429022	33.56350389137274	19.111253045679273
20	20.28103044496487	27.353393418967187	32.157168878480356	20.208407257587584
21	14.97598940221891	33.25432309038867	34.38530504104275	17.384382466349678
22	14.662787121803516	26.90629952925035	30.318406547914744	28.11250680103139
23	13.606320819435574	32.142738864050344	31.26984126984127	22.981099046672817
24	16.180540770704706	25.536394388853406	33.43079507013933	24.852269770302556
25	13.655997918293	29.64421734913538	31.016724623282	25.683060109289617
26	13.617439027275093	29.225510373051357	33.37709649185059	23.77995410782296
27	19.282047642703382	29.967828164549477	29.12402715681404	21.6260970359331
28	13.791545430889693	26.34187306318454	35.81718827620467	24.0493932297211
29	15.646393679180564	25.86047831949471	31.04936957395974	27.443758427364983
30	20.89868237409221	27.18212570671587	28.135926004778465	23.783265914413455
31	19.80673242968325	30.75698436354174	23.537245996262392	25.89903721051262
32	19.580583351075155	28.794975516286993	24.869538476095855	26.754902656542
33	20.798145388309322	29.386842665531187	25.02850519243962	24.78650675371987
34	15.711920138149646	27.70018688051475	28.354268682137533	28.23362429919807
35	16.916944621862655	27.606036950299245	28.24379627658316	27.23322215125494
36	19.57206727698531	28.398504955882004	29.27471435668157	22.754713410451117
37	19.836775246611314	28.38029001963428	30.95829489272112	20.824639841033285
38	17.00872897594209	32.71307927045632	27.996830127977667	22.281361625623923
39	18.9485014075178	27.905282331511838	32.17254512336479	20.973671137605564
40	21.921084380100776	29.688926738107067	30.133655051687843	18.25633383010432
41	17.086556430818725	28.43635417405909	27.900314621626098	26.576774773496087
42	22.708111560570575	24.138812007664466	30.154472121685238	22.99860431007972
43	23.03195893359828	20.257138125990583	33.96281314314101	22.748089797270126
44	20.26920256428453	23.61696591204788	27.836444065952264	28.277387457715324
45	19.302391597473566	22.82307856078348	30.464126037896527	27.410403803846428
46	23.682965486244175	26.643957135760417	27.87027180469803	21.802805573297377
47	16.569678045087883	25.656802214179265	33.72388995339815	24.049629787334705
48	17.801670096752066	23.506730064107114	32.45073687696639	26.24086296217444
49	21.009864452487403	18.29844108532633	36.081659688217066	24.6100347739692
50	18.034206230927545	19.652260307998013	35.71168358053604	26.601849880538403
51	15.928370354599863	19.494949494949495	29.445508953705673	35.131171196744965
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1048.0
1	904.5
2	761.0
3	3557.0
4	6353.0
5	4436.0
6	2519.0
7	2641.5
8	2764.0
9	3032.0
10	3300.0
11	3530.5
12	3761.0
13	3629.5
14	3498.0
15	3547.0
16	3596.0
17	3530.5
18	3465.0
19	3519.5
20	3574.0
21	3336.0
22	3098.0
23	3306.5
24	3515.0
25	3551.5
26	3824.5
27	4061.0
28	4772.0
29	5483.0
30	5944.0
31	6405.0
32	6446.5
33	6488.0
34	6824.0
35	7160.0
36	7438.0
37	7716.0
38	8994.0
39	10272.0
40	14670.5
41	19069.0
42	26444.5
43	33820.0
44	45274.5
45	56729.0
46	60786.5
47	64844.0
48	58803.0
49	52762.0
50	43311.5
51	33861.0
52	27527.5
53	21194.0
54	17694.5
55	14195.0
56	12209.0
57	10223.0
58	9036.0
59	7849.0
60	6789.5
61	5730.0
62	5003.0
63	4276.0
64	3348.5
65	2421.0
66	1827.5
67	1234.0
68	1099.0
69	964.0
70	758.0
71	552.0
72	384.5
73	217.0
74	202.5
75	127.5
76	67.0
77	64.0
78	61.0
79	43.0
80	25.0
81	14.0
82	3.0
83	2.0
84	1.0
85	9.5
86	18.0
87	9.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	422730.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.84830267975022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.77306658965718	27.92108892668556
2	9.847155436927164	7.257019281488803
3	4.083773319033443	4.514403460056974
4	2.187330873754197	3.223977203874287
5	1.3924244061255289	2.5654237987792463
6	0.9313020806778469	2.059014057305907
7	0.683126487713021	1.7620436111462845
8	0.5116457655150609	1.5082622426009178
9	0.44072901273189924	1.461610445480326
>10	3.709612420608125	29.97463834355414
>50	0.3544541423184772	8.387662257568582
>100	0.07567725301325151	4.7401064724518065
>500	0.003880884769910334	0.8932984241124986
>1k	0.005821327154865502	3.7314514748946843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4022	0.9514347219265253	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	2402	0.568211387883519	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1870	0.44236273744470467	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1516	0.3586213422278996	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	1225	0.2897830766683226	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1211	0.28647127007782747	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1158	0.2739337165566674	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	1148	0.27156814042059946	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	1104	0.26115960542190053	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	938	0.22189104156317271	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	667	0.15778392827573157	No Hit
AAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	563	0.13318193646062498	No Hit
CAGAGCGTCTTTTAAAAAGTTGCAATGGTTTGCTTTATAGAAACAATCTTT	539	0.12750455373406194	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	525	0.12419274714356682	No Hit
GAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	516	0.12206372862110568	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	453	0.10716059896387765	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3655761360679393E-4	0.0	0.0	0.6500603221914697	0.0
2	2.3655761360679393E-4	0.0	0.0	2.229318950630426	0.0
3	2.3655761360679393E-4	0.0	0.0	3.105055236202777	0.0
4	2.3655761360679393E-4	0.0	0.0	4.801409883377096	0.0
5	2.3655761360679393E-4	0.0	0.0	8.378397558725428	0.0
6	2.3655761360679393E-4	0.0	0.0	10.941735859768647	0.0
7	2.3655761360679393E-4	0.0	0.0	12.752347834315048	0.0
8	2.3655761360679393E-4	0.0	0.0	17.286684171930073	0.0
9	2.3655761360679393E-4	0.0	0.0	18.918222032976132	0.0
10	2.3655761360679393E-4	0.0	0.0	22.55742436070305	0.0
11	2.3655761360679393E-4	0.0	0.0	26.539398670546213	0.0
12	2.3655761360679393E-4	0.0	0.0	30.494878527665414	0.0
13	2.3655761360679393E-4	0.0	0.0	31.62183899888818	0.0
14	2.3655761360679393E-4	0.0	0.0	31.916589785442245	0.0
15	2.3655761360679393E-4	0.0	0.0	32.55198353559009	0.0
16	2.3655761360679393E-4	0.0	0.0	33.98268398268398	0.0
17	2.3655761360679393E-4	0.0	0.0	35.431599366025594	0.0
18	0.0023655761360679393	0.0	0.0	38.576159723700705	0.0
19	0.0023655761360679393	0.0	0.0	39.56733612471317	0.0
20	0.0023655761360679393	0.0	0.0	40.85042462091642	0.0
21	0.0023655761360679393	0.0	0.0	41.75691339625766	0.0
22	0.0023655761360679393	0.0	0.0	42.469898043668536	0.0
23	0.0023655761360679393	0.0	0.0	43.195893359827785	0.0
24	0.0023655761360679393	0.0	0.0	43.784685260095095	0.0
25	0.0023655761360679393	0.0	0.0	44.22042438435881	0.0
26	0.0023655761360679393	0.0	0.0	44.621389539422324	0.0
27	0.0023655761360679393	0.0	0.0	45.0001182788068	0.0
28	0.0023655761360679393	0.0	0.0	45.35235256546732	0.0
29	0.0023655761360679393	0.0	0.0	45.74196295507771	0.0
30	0.0023655761360679393	0.0	0.0	46.190003075248974	0.0
31	0.0023655761360679393	0.0	0.0	46.63094646701204	0.0
32	0.0023655761360679393	0.0	0.0	47.01511603150947	0.0
33	0.0026021337496747333	0.0	0.0	47.38225344782722	0.0
34	0.0026021337496747333	0.0	0.0	47.71272443403591	0.0
35	0.002838691363281527	0.0	0.0	48.122678778416486	0.0
36	0.002838691363281527	0.0	0.0	48.457644359283705	0.0
37	0.002838691363281527	0.0	0.0	48.78267452037944	0.0
38	0.002838691363281527	0.0	0.0	49.121898138291584	0.0
39	0.002838691363281527	0.0	0.0	49.455444373477164	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	125	0.0	45.000004	5
GCGACGG	35	1.2094824E-7	45.000004	2
TACATCA	45	3.8380676E-10	45.000004	24
GTGCTAG	45	3.8380676E-10	45.000004	1
AGTTGCA	70	0.0	45.000004	18
GCGTCTT	70	0.0	45.000004	5
TCACGAC	20	7.028221E-4	45.0	25
GTCATCC	20	7.028221E-4	45.0	10
CTATGCG	20	7.028221E-4	45.0	1
ACCGGGA	40	6.7975634E-9	45.0	4
CTCGTAG	20	7.028221E-4	45.0	1
GTCTGGC	25	3.886607E-5	45.0	14
CGACGGT	20	7.028221E-4	45.0	28
CGTGCAA	20	7.028221E-4	45.0	34
CAGCCCG	20	7.028221E-4	45.0	14
AGCCCAT	20	7.028221E-4	45.0	27
GAACTTC	20	7.028221E-4	45.0	8
TTAGAGC	20	7.028221E-4	45.0	2
GGCAACT	25	3.886607E-5	45.0	8
GGCAACG	40	6.7975634E-9	45.0	8
>>END_MODULE