SRR3548202_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421242
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	4441	1.054263345060559	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	4144	0.9837575550396209	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	4085	0.9697513543283909	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3357	0.7969290811457548	No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1963	0.46600291518889375	RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	1177	0.2794118345274213	RNA PCR Primer, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1087	0.25804644361198553	RNA PCR Primer, Index 38 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC	1031	0.2447524225979366	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT	814	0.19323809116849697	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT	741	0.1759083852037546	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	687	0.16308915065449314	RNA PCR Primer, Index 38 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA	630	0.14955773640805048	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGACTATGT	555	0.13175324397852067	No Hit
AGATTTGGGAAAAGATTTTTTTTACCAACCCTACATCTGATAGAGGGCTAA	515	0.12225751468277143	No Hit
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	495	0.1175096500348968	RNA PCR Primer, Index 27 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	35	1.2094824E-7	45.000004	2
GTTGACG	20	7.0281903E-4	45.000004	1
GTCATGC	20	7.0281903E-4	45.000004	24
CTATGCA	35	1.2094824E-7	45.000004	29
ACCGGAC	40	6.7975634E-9	45.000004	43
ATTAGCG	20	7.0281903E-4	45.000004	1
CTCCGTC	20	7.0281903E-4	45.000004	21
CTCAAAC	20	7.0281903E-4	45.000004	34
CGACGGT	20	7.0281903E-4	45.000004	28
AGCCCTT	20	7.0281903E-4	45.000004	25
CGTGACC	35	1.2094824E-7	45.000004	11
GACCGTA	20	7.0281903E-4	45.000004	41
AACCACG	20	7.0281903E-4	45.000004	12
TAGCGCG	20	7.0281903E-4	45.000004	2
ACCGAAC	20	7.0281903E-4	45.000004	11
GTGATGA	20	7.0281903E-4	45.000004	45
GTGATCG	20	7.0281903E-4	45.000004	1
CACGACG	20	7.0281903E-4	45.000004	26
TAACGGG	80	0.0	45.000004	3
CCTCGGA	20	7.0281903E-4	45.000004	23