##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421242
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.435319839902004	28.0	25.0	31.0	16.0	33.0
2	27.311858741530997	30.0	25.0	31.0	16.0	33.0
3	27.201774751805374	30.0	25.0	31.0	16.0	33.0
4	30.282367380270724	33.0	28.0	35.0	19.0	37.0
5	31.69015910094435	33.0	30.0	35.0	25.0	37.0
6	30.803604578840666	33.0	29.0	35.0	17.0	37.0
7	31.80132085594504	33.0	30.0	35.0	25.0	35.0
8	33.10114850845832	35.0	33.0	35.0	28.0	37.0
9	34.16204936829661	37.0	32.0	38.0	27.0	39.0
10	33.811431433712684	35.0	32.0	38.0	27.0	39.0
11	34.03989868056841	37.0	32.0	39.0	27.0	39.0
12	33.49444025049734	35.0	31.0	38.0	25.0	39.0
13	33.674151675284044	35.0	32.0	39.0	25.0	39.0
14	34.30658148997488	37.0	32.0	39.0	25.0	40.0
15	35.39165847660015	37.0	33.0	40.0	27.0	41.0
16	35.35215624272983	37.0	33.0	40.0	27.0	41.0
17	34.954031174479276	36.0	33.0	39.0	27.0	40.0
18	34.735892907165	36.0	33.0	39.0	27.0	40.0
19	34.16881507541983	36.0	32.0	39.0	26.0	40.0
20	34.23624899701359	35.0	32.0	38.0	27.0	40.0
21	34.3464113265059	35.0	33.0	39.0	27.0	40.0
22	34.68472516985486	36.0	33.0	39.0	27.0	40.0
23	35.0919827557556	36.0	33.0	39.0	29.0	40.0
24	34.73077233514227	36.0	33.0	39.0	27.0	40.0
25	33.81285816703937	35.0	32.0	39.0	24.0	40.0
26	33.83610608628769	35.0	32.0	39.0	24.0	40.0
27	34.332490587358336	35.0	33.0	39.0	25.0	40.0
28	34.15798044829338	36.0	33.0	39.0	24.0	40.0
29	34.20847398882353	36.0	33.0	39.0	24.0	40.0
30	33.58431970221393	35.0	32.0	39.0	22.0	40.0
31	33.459766594973914	35.0	32.0	39.0	22.0	40.0
32	33.081724994183865	35.0	32.0	39.0	19.0	40.0
33	32.942389410362686	36.0	32.0	39.0	18.0	40.0
34	32.62242368994545	36.0	31.0	39.0	15.0	40.0
35	32.58717554279963	36.0	31.0	40.0	13.0	40.0
36	32.2093737091743	36.0	31.0	39.0	10.0	40.0
37	32.19451289282645	36.0	31.0	39.0	10.0	40.0
38	32.09377507466017	36.0	31.0	39.0	10.0	40.0
39	31.90298213378533	35.0	30.0	39.0	10.0	40.0
40	31.817807815934785	35.0	30.0	39.0	10.0	40.0
41	31.53867373148926	35.0	30.0	39.0	10.0	40.0
42	31.685874153099643	35.0	30.0	39.0	10.0	40.0
43	31.626654037346704	35.0	30.0	39.0	10.0	40.0
44	31.492322702864385	35.0	30.0	39.0	10.0	40.0
45	31.590375603572294	35.0	30.0	39.0	10.0	40.0
46	31.341105587761906	35.0	29.0	39.0	10.0	40.0
47	31.444737229431063	35.0	30.0	39.0	10.0	40.0
48	31.298574216246244	35.0	29.0	39.0	10.0	40.0
49	31.31702204433556	35.0	30.0	39.0	10.0	40.0
50	31.18623261688056	35.0	29.0	39.0	9.0	40.0
51	29.214843249248652	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	24.0
10	34.0
11	25.0
12	26.0
13	30.0
14	70.0
15	175.0
16	411.0
17	840.0
18	1702.0
19	2654.0
20	3848.0
21	5540.0
22	6893.0
23	8747.0
24	10393.0
25	12397.0
26	13341.0
27	13291.0
28	13256.0
29	14859.0
30	18104.0
31	22612.0
32	28821.0
33	35691.0
34	40365.0
35	41064.0
36	47206.0
37	47278.0
38	28422.0
39	3112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.38874566163868	21.456075130210188	24.17945029223107	21.975728915920065
2	36.019200364636006	25.59336438436813	26.048447210866915	12.338988040128953
3	26.153849806049728	24.92533982841217	36.98016816936583	11.940642196172272
4	23.035689698558073	25.302082888221022	37.32723707512547	14.33499033809544
5	22.360780738862694	28.649564858205025	34.61881768674539	14.370836716186894
6	18.210672250155493	37.753120534039816	33.95838971422603	10.077817501578664
7	70.3842446859525	5.793581836569003	21.02924209836626	2.792931379112244
8	68.23607332602163	8.546631152639101	18.191680791563993	5.025614729775284
9	63.18790623916893	6.943277261051842	20.813926436585145	9.054890063194078
10	36.01777600524164	25.800133889783073	25.595738316692067	12.58635178828322
11	30.827647765417503	23.76258777614768	31.587068715845053	13.822695742589769
12	27.91768152273515	18.906234421069122	36.84034355548592	16.335740500709804
13	20.608343897332173	28.70107918963446	36.29671305330427	14.393863859729086
14	14.899511444727734	34.111745742352376	34.053584400415915	16.935158412503977
15	11.939692623242697	23.78632709938705	49.769491171345685	14.504489106024565
16	13.410818484386647	23.48792380626813	43.632401327502954	19.46885638184227
17	14.116351171060815	22.7657735933264	36.2957634803747	26.822111755238083
18	17.07925610456697	23.863717293147406	39.4087009367537	19.648325665531928
19	20.619976165719468	25.11121872937646	35.73337891283395	18.53542619207012
20	22.384757455334466	22.681261602594233	36.73209224151438	18.201888700556925
21	18.360942166260724	27.763375921679224	37.185038528921616	16.690643383138433
22	17.554992142284007	24.368177911984084	35.305358914828055	22.77147103090385
23	15.643027048584898	28.321487411036887	35.24268710147611	20.792798438902103
24	17.827519573072014	23.255041045289882	38.33995660451712	20.577482777120988
25	15.170614516121375	26.95362760598421	37.1518034763865	20.72395440150792
26	15.097022614079316	26.640268539224483	35.452067932447385	22.81064091424882
27	17.70597423808642	29.994160126483116	33.393393821128946	18.90647181430152
28	14.369174963560138	27.741298351066607	39.785918783027334	18.10360790234592
29	16.39770013436457	23.858019855569957	36.07783649303726	23.666443517028217
30	18.65792109998528	27.024608182469933	34.79069038699845	19.526780330546337
31	20.85998072366953	29.515575369977356	29.65682434325162	19.967619563101497
32	21.472455263245354	27.164907582814628	30.99975785890296	20.362879295037057
33	21.879584656800606	25.818887955142173	30.909548430593343	21.39197895746388
34	18.834066878421428	27.935248621932285	31.50374369127485	21.726940808371435
35	19.64903784522911	24.49518329131473	31.103261308226628	24.752517555229538
36	25.248194624467647	24.774832519074547	30.360932670531426	19.61604018592638
37	21.321473167442942	27.975368078206824	31.302434230204966	19.400724524145264
38	19.35965549494115	28.589979156874197	30.234402077665568	21.815963270519084
39	22.50796454294681	25.31585169569986	31.099463016508327	21.076720744845005
40	22.04932081796212	23.917130770435996	33.704378955564735	20.329169456037146
41	18.76664720042161	26.248569705774827	30.55464554816471	24.43013754563885
42	21.833292976483825	24.861481048898256	30.99026212960721	22.31496384501071
43	24.605096357913027	24.332568927125024	30.402951272665117	20.65938344229683
44	19.696516491707854	27.28194244638474	29.843415423913093	23.178125637994313
45	17.522944055910855	27.566814325257216	29.633085020012253	25.277156598819683
46	23.226316464170242	25.895328575972954	30.469658770967754	20.408696188889046
47	19.034901553026526	25.268135655988722	34.082308981535554	21.614653809449198
48	20.125486062643326	23.28613955873346	32.83979280318677	23.748581575436447
49	21.467470005365087	21.961247928744047	34.109371810028435	22.461910255862424
50	19.355619809990458	23.715346522901328	33.74663495093082	23.1823987161774
51	18.361654345957906	24.925577221644566	29.05859339761942	27.65417503477811
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	185.0
2	304.0
3	4049.0
4	7794.0
5	6624.0
6	5454.0
7	5632.0
8	5810.0
9	6104.5
10	6399.0
11	6337.5
12	6276.0
13	6129.5
14	5983.0
15	5708.5
16	5434.0
17	5432.0
18	5430.0
19	4965.5
20	4501.0
21	4238.5
22	3976.0
23	3901.5
24	3827.0
25	3690.0
26	3697.5
27	3842.0
28	4184.5
29	4527.0
30	5471.0
31	6415.0
32	7222.0
33	8029.0
34	9233.5
35	10438.0
36	10677.0
37	10916.0
38	12200.5
39	13485.0
40	15918.0
41	18351.0
42	22730.0
43	27109.0
44	31773.0
45	36437.0
46	44806.0
47	53175.0
48	46755.0
49	40335.0
50	37157.5
51	33980.0
52	28197.0
53	22414.0
54	19627.0
55	16840.0
56	15073.5
57	13307.0
58	12457.5
59	11608.0
60	10339.5
61	9071.0
62	7903.0
63	6735.0
64	5242.0
65	3749.0
66	3085.5
67	2422.0
68	1840.5
69	1259.0
70	1090.5
71	922.0
72	665.5
73	409.0
74	369.0
75	272.5
76	216.0
77	128.0
78	40.0
79	43.0
80	46.0
81	28.0
82	10.0
83	7.0
84	4.0
85	2.5
86	1.0
87	2.0
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	4.0
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	421242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.575940999385914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.87793846267238	32.35670405286172
2	7.6138043804067195	5.8741933711887135
3	2.4674456686191335	2.855521155955261
4	1.1674991283373184	1.801495099662995
5	0.677628372766605	1.3070076063677223
6	0.434405973918128	1.0054571531787893
7	0.29819164176829754	0.8052116225554709
8	0.22080592468594723	0.6814237058399961
9	0.18003199536008688	0.6250413267911187
>10	2.1675185885437718	20.963266465443272
>50	0.792260800408269	20.8507516353595
>100	0.09372170351832834	4.793542198687171
>500	0.003748868140733134	0.9501278835088911
>1k	0.004998490854310845	5.130256722599378
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	4441	1.054263345060559	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	4144	0.9837575550396209	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	4085	0.9697513543283909	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3357	0.7969290811457548	No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1963	0.46600291518889375	RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	1177	0.2794118345274213	RNA PCR Primer, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1087	0.25804644361198553	RNA PCR Primer, Index 38 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC	1031	0.2447524225979366	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT	814	0.19323809116849697	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT	741	0.1759083852037546	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	687	0.16308915065449314	RNA PCR Primer, Index 38 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA	630	0.14955773640805048	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGACTATGT	555	0.13175324397852067	No Hit
AGATTTGGGAAAAGATTTTTTTTACCAACCCTACATCTGATAGAGGGCTAA	515	0.12225751468277143	No Hit
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	495	0.1175096500348968	RNA PCR Primer, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.373932323937309E-4	0.0	0.0	0.3045755171611568	0.0
2	2.373932323937309E-4	0.0	0.0	1.3056627781655201	0.0
3	2.373932323937309E-4	0.0	0.0	1.601217352495715	0.0
4	2.373932323937309E-4	0.0	0.0	2.350905180395117	0.0
5	4.747864647874618E-4	0.0	0.0	4.506198337297801	0.0
6	4.747864647874618E-4	0.0	0.0	5.449361649598093	0.0
7	4.747864647874618E-4	0.0	0.0	6.230147990941075	0.0
8	4.747864647874618E-4	0.0	0.0	7.899971987598578	0.0
9	4.747864647874618E-4	0.0	0.0	8.417726627449305	0.0
10	4.747864647874618E-4	0.0	0.0	10.418951576528457	0.0
11	4.747864647874618E-4	0.0	0.0	11.63321796022239	0.0
12	4.747864647874618E-4	0.0	0.0	13.903884228068426	0.0
13	4.747864647874618E-4	0.0	0.0	14.63909106879181	0.0
14	4.747864647874618E-4	0.0	0.0	14.9310847446361	0.0
15	4.747864647874618E-4	0.0	0.0	15.392339795177119	0.0
16	4.747864647874618E-4	0.0	0.0	16.104044705893525	0.0
17	4.747864647874618E-4	0.0	0.0	16.765422251342457	0.0
18	0.0011869661619686547	0.0	0.0	18.086278196381176	0.0
19	0.0011869661619686547	0.0	0.0	18.608543307647384	0.0
20	0.0011869661619686547	0.0	0.0	19.12558576780093	0.0
21	0.0011869661619686547	0.0	0.0	19.537700419236447	0.0
22	0.0011869661619686547	0.0	0.0	19.886905864087627	0.0
23	0.0011869661619686547	0.0	0.0	20.26887157500914	0.0
24	0.0011869661619686547	0.0	0.0	20.545909477212625	0.0
25	0.0011869661619686547	0.0	0.0	20.773806980310606	0.0
26	0.0011869661619686547	0.0	0.0	21.012149785633913	0.0
27	0.0011869661619686547	0.0	0.0	21.228652413576995	0.0
28	0.0011869661619686547	0.0	0.0	21.439932390407414	0.0
29	0.0011869661619686547	0.0	0.0	21.686346565632107	0.0
30	0.0014243593943623855	0.0	0.0	21.962909681370803	0.0
31	0.0014243593943623855	0.0	0.0	22.23448753922923	0.0
32	0.0014243593943623855	0.0	0.0	22.477578209200413	0.0
33	0.0018991458591498473	0.0	0.0	22.730876788164522	0.0
34	0.0018991458591498473	0.0	0.0	22.993196309959597	0.0
35	0.0018991458591498473	0.0	0.0	23.229402576191358	0.0
36	0.0018991458591498473	0.0	0.0	23.457062686056947	0.0
37	0.0018991458591498473	0.0	0.0	23.7124978041126	0.0
38	0.0018991458591498473	0.0	0.0	23.96508420337953	0.0
39	0.0018991458591498473	0.0	0.0	24.245208217604134	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	35	1.2094824E-7	45.000004	2
GTTGACG	20	7.0281903E-4	45.000004	1
GTCATGC	20	7.0281903E-4	45.000004	24
CTATGCA	35	1.2094824E-7	45.000004	29
ACCGGAC	40	6.7975634E-9	45.000004	43
ATTAGCG	20	7.0281903E-4	45.000004	1
CTCCGTC	20	7.0281903E-4	45.000004	21
CTCAAAC	20	7.0281903E-4	45.000004	34
CGACGGT	20	7.0281903E-4	45.000004	28
AGCCCTT	20	7.0281903E-4	45.000004	25
CGTGACC	35	1.2094824E-7	45.000004	11
GACCGTA	20	7.0281903E-4	45.000004	41
AACCACG	20	7.0281903E-4	45.000004	12
TAGCGCG	20	7.0281903E-4	45.000004	2
ACCGAAC	20	7.0281903E-4	45.000004	11
GTGATGA	20	7.0281903E-4	45.000004	45
GTGATCG	20	7.0281903E-4	45.000004	1
CACGACG	20	7.0281903E-4	45.000004	26
TAACGGG	80	0.0	45.000004	3
CCTCGGA	20	7.0281903E-4	45.000004	23
>>END_MODULE