SRR3548201_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548201_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384686
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	3556	0.9243902819442351	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3171	0.8243086569305875	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	3107	0.8076717114737734	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	2807	0.7296860296449572	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	2749	0.7146087978247194	No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	2351	0.6111477932651566	RNA PCR Primer, Index 38 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1486	0.3862890773254031	RNA PCR Primer, Index 38 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1368	0.35561470913940196	RNA PCR Primer, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1102	0.2864674045845183	RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	1033	0.26853069776389055	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT	955	0.24825442048839833	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC	837	0.2175800523023973	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	824	0.2142006727564819	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGACTATGT	704	0.1830064000249554	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT	692	0.17988697275180277	No Hit
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	566	0.14713298638369995	RNA PCR Primer, Index 38 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	562	0.1460931772926491	No Hit
ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	498	0.12945623183583493	RNA PCR Primer, Index 27 (95% over 22bp)
AGATTTGGGAAAAGATTTTTTTTACCAACCCTACATCTGATAGAGGGCTAA	469	0.12191761592571604	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA	459	0.11931809319808884	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	20	7.027427E-4	45.000004	36
CTTGTAG	40	6.7939254E-9	45.000004	1
TATCACG	20	7.027427E-4	45.000004	1
GCCCACA	20	7.027427E-4	45.000004	11
CAAACCG	20	7.027427E-4	45.000004	26
AATCAAT	20	7.027427E-4	45.000004	25
GAGTACT	20	7.027427E-4	45.000004	9
GTCTCAC	20	7.027427E-4	45.000004	22
CGTCTGG	20	7.027427E-4	45.000004	2
GTTAGCG	40	6.7939254E-9	45.000004	1
GTCTAAG	20	7.027427E-4	45.000004	1
AGTATCA	20	7.027427E-4	45.000004	37
ATCGTAG	20	7.027427E-4	45.000004	1
ATCAACT	20	7.027427E-4	45.000004	21
TATTGCG	20	7.027427E-4	45.000004	1
CCTCAAA	30	2.1617216E-6	45.000004	23
TACCTTA	20	7.027427E-4	45.000004	43
ATCCACT	20	7.027427E-4	45.000004	19
GTATACG	20	7.027427E-4	45.000004	1
TGTAGAC	20	7.027427E-4	45.000004	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content