##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548201_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 384686 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.005607170523493 28.0 25.0 31.0 16.0 33.0 2 27.447089314401875 30.0 25.0 33.0 16.0 34.0 3 27.2951316138357 30.0 25.0 33.0 16.0 34.0 4 31.70309291214133 33.0 28.0 35.0 19.0 37.0 5 32.94684235974275 35.0 32.0 35.0 28.0 37.0 6 31.615691758993048 35.0 31.0 35.0 19.0 37.0 7 33.58823300042112 35.0 33.0 35.0 30.0 37.0 8 33.3203391857255 35.0 33.0 37.0 28.0 37.0 9 35.85099535725241 37.0 35.0 39.0 30.0 39.0 10 35.2156512064385 37.0 34.0 39.0 30.0 39.0 11 35.664887726613394 37.0 35.0 39.0 31.0 39.0 12 35.71686258402957 37.0 35.0 39.0 31.0 39.0 13 35.70243783241397 37.0 35.0 39.0 31.0 39.0 14 36.28438778640242 38.0 35.0 40.0 31.0 41.0 15 36.806598108587266 38.0 35.0 40.0 32.0 41.0 16 36.35834940704886 38.0 34.0 40.0 31.0 41.0 17 36.05717910191689 38.0 34.0 40.0 31.0 41.0 18 36.08058780407917 38.0 34.0 39.0 31.0 40.0 19 35.59299272653541 37.0 34.0 39.0 29.0 40.0 20 35.9096145947604 37.0 34.0 39.0 30.0 40.0 21 35.92034542458005 37.0 34.0 39.0 30.0 40.0 22 36.39233816671259 38.0 35.0 40.0 31.0 41.0 23 36.598059196331555 38.0 35.0 40.0 31.0 41.0 24 36.58871391212573 38.0 35.0 40.0 31.0 41.0 25 35.73466931471382 38.0 34.0 40.0 29.0 41.0 26 36.01710226002506 38.0 34.0 40.0 30.0 41.0 27 36.2276298071674 38.0 35.0 40.0 31.0 41.0 28 35.97432711354195 38.0 35.0 40.0 30.0 41.0 29 36.04692658427913 38.0 35.0 40.0 30.0 41.0 30 35.38807754896201 38.0 34.0 40.0 27.0 41.0 31 35.31977248977088 38.0 34.0 40.0 27.0 41.0 32 35.18571770222987 38.0 34.0 40.0 27.0 41.0 33 35.1251436236307 38.0 34.0 40.0 25.0 41.0 34 34.87936654830173 38.0 34.0 40.0 24.0 41.0 35 34.908023686851095 38.0 34.0 40.0 24.0 41.0 36 34.570249502191395 38.0 34.0 40.0 22.0 41.0 37 34.55233619107532 38.0 34.0 40.0 22.0 41.0 38 34.436202513218575 38.0 34.0 40.0 21.0 41.0 39 34.32880583124938 38.0 34.0 40.0 21.0 41.0 40 34.25211991078438 38.0 33.0 40.0 21.0 41.0 41 33.88984262489407 37.0 33.0 40.0 18.0 41.0 42 34.0934528420582 38.0 33.0 40.0 20.0 41.0 43 34.02946299059493 38.0 33.0 40.0 18.0 41.0 44 33.99415367338557 38.0 33.0 40.0 18.0 41.0 45 34.1244755462897 38.0 34.0 40.0 20.0 41.0 46 33.83584793831852 37.0 33.0 40.0 19.0 41.0 47 33.77455899096926 37.0 33.0 40.0 20.0 41.0 48 33.86175738134479 37.0 33.0 40.0 20.0 41.0 49 33.8627841928222 37.0 33.0 40.0 19.0 41.0 50 33.67677014500138 37.0 33.0 40.0 18.0 41.0 51 31.468288422245674 35.0 29.0 38.0 13.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 7.0 10 24.0 11 15.0 12 13.0 13 25.0 14 19.0 15 52.0 16 113.0 17 256.0 18 571.0 19 1216.0 20 2144.0 21 2959.0 22 3759.0 23 4309.0 24 4845.0 25 5728.0 26 6794.0 27 7127.0 28 7063.0 29 7649.0 30 9435.0 31 12934.0 32 18295.0 33 27199.0 34 36568.0 35 42687.0 36 59291.0 37 69381.0 38 45777.0 39 8431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.83000161170409 24.526496935162704 25.350545639820528 14.292955813312677 2 32.2735945680373 27.64098511513286 25.913862214897343 14.171558101932485 3 26.426488096785427 27.647743874224695 32.28347275440229 13.642295274587585 4 23.91378942826097 27.207904628710168 33.73946543414629 15.13884050888257 5 22.586213171261758 30.14380559729234 30.562328756440316 16.707652475005588 6 18.696807266185928 37.909359841533096 29.920246642716396 13.473586249564578 7 70.08781187773924 7.3530099873663195 17.71184810468798 4.847330030206455 8 65.28259411572034 8.87503054439205 15.714114888506469 10.128260451381127 9 59.123284964880455 8.79574510119942 20.68232272554759 11.398647208372543 10 33.0929641317854 27.448360481015687 23.772895296423577 15.685780090775333 11 25.677825551228793 26.484197501338752 30.022927790457672 17.81504915697478 12 23.293283353176356 19.215152098074796 37.19215152098075 20.2994130277681 13 22.02939540300401 27.003062237773147 36.07669631855591 14.890846040666933 14 14.626994483812773 34.39220559105348 30.98865048377118 19.992149441362567 15 10.910976744669679 25.946876153538213 45.85532096307118 17.286826138720933 16 11.346916706092761 28.030653572004184 37.88752385062102 22.73490587128203 17 12.224255626666944 27.607971176491997 31.35856256791253 28.80921062892853 18 15.613253406674534 25.710059633051372 35.993771543544604 22.682915416729486 19 17.817648679702405 28.657658453907864 32.638567558996165 20.886125307393563 20 19.98383096863416 26.383076067234057 32.87720374539235 20.75588921873944 21 17.04585038186989 31.90030310435004 33.68175602959297 17.3720904841871 22 14.910342461124138 27.50632983784177 30.608340308719317 26.97498739231477 23 14.152321633748045 30.86543310648165 31.106668815605452 23.87557644416485 24 17.975179756996614 25.585542494398027 32.177932131660626 24.26134561694473 25 14.314011947406458 30.195016195026593 29.817045590429597 25.673926267137354 26 14.202232470118487 28.367811669777428 31.51401402702464 25.915941833079447 27 19.765471059513473 30.307055624587324 27.81697280379322 22.110500512105975 28 14.690942742912402 29.04810676759747 33.325361463635275 22.935589025854853 29 16.28730964994827 25.01546716022938 29.42113827901197 29.276084910810376 30 21.363917584731443 27.69063599923054 28.168948181113944 22.776498234924066 31 19.599361557218096 33.50420862729603 23.24961137135222 23.646818444133658 32 19.4758842276558 28.985458269861653 24.901868017032072 26.636789485450475 33 22.16041134847642 26.267397305854644 26.912078942306195 24.660112403362746 34 17.6754547865012 28.397966133417906 29.02965015623132 24.896928923849583 35 19.25492479580749 23.956941505539582 27.34463952418336 29.44349417446957 36 24.9177251056706 24.273563373764574 29.93896320635531 20.86974831420951 37 22.353035982593596 27.980222831088213 28.86536031984528 20.801380866472915 38 19.069318873054907 27.770701299241455 29.50302324493223 23.6569565827714 39 24.422775978330378 23.222316382712137 29.153387438066368 23.201520200891114 40 23.44821490774294 22.62598586899445 33.67031812959141 20.2554810936712 41 20.073514502737297 24.926823435217294 28.643880983451435 26.35578107859397 42 24.956198042039482 22.337958750773357 29.770254181332305 22.935589025854853 43 27.260674940081003 20.88014640512002 29.719043583598054 22.140135071200927 44 20.54090868916467 25.033663819322772 27.80475504697338 26.620672444539185 45 18.71240440255169 22.136235787109488 28.976099988042197 30.175259822296624 46 24.61670037381137 22.4889910212485 30.535553672345756 22.358754932594376 47 18.221874463848437 23.320578341816443 34.40676291832819 24.050784276006926 48 18.84601987075173 20.330867252772393 33.399187909099886 27.42392496737599 49 23.154728791793826 17.867819468345612 34.64409934336056 24.3333523965 50 19.751693589057048 18.336253463864036 35.695346334413 26.216706612665913 51 18.340412700228235 19.155103123066606 28.732524708463526 33.77195946824163 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 964.0 1 880.5 2 797.0 3 2956.0 4 5115.0 5 3635.5 6 2156.0 7 2253.0 8 2350.0 9 2566.0 10 2782.0 11 2852.5 12 2923.0 13 3024.5 14 3126.0 15 3091.5 16 3057.0 17 3095.5 18 3134.0 19 3034.5 20 2935.0 21 2804.5 22 2674.0 23 2641.5 24 2609.0 25 2661.5 26 2999.5 27 3285.0 28 3692.5 29 4100.0 30 4616.0 31 5132.0 32 5684.5 33 6237.0 34 6856.0 35 7475.0 36 7560.0 37 7645.0 38 8424.0 39 9203.0 40 11136.0 41 13069.0 42 17394.0 43 21719.0 44 27593.5 45 33468.0 46 44426.0 47 55384.0 48 55606.5 49 55829.0 50 50576.0 51 45323.0 52 35690.5 53 26058.0 54 21218.5 55 16379.0 56 13870.5 57 11362.0 58 9989.5 59 8617.0 60 7531.0 61 6445.0 62 5554.0 63 4663.0 64 3564.0 65 2465.0 66 2045.0 67 1625.0 68 1212.5 69 800.0 70 660.5 71 521.0 72 361.0 73 201.0 74 193.0 75 149.0 76 113.0 77 67.0 78 21.0 79 19.0 80 17.0 81 10.0 82 3.0 83 2.5 84 2.0 85 1.5 86 1.0 87 1.0 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 384686.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.775615318232646 #Duplication Level Percentage of deduplicated Percentage of total 1 76.82108846568647 28.251427977420047 2 9.778681175141243 7.19234034433275 3 3.7678883391844655 4.156992363717072 4 2.067094004408667 3.0407461573103296 5 1.2732812889607692 2.341285143736234 6 0.8317034558173323 1.835184380998975 7 0.6309872178505102 1.6243460236074554 8 0.4610848180545156 1.3565342318280125 9 0.3455786861558635 1.1437981941823459 >10 3.6286172748450705 30.348178143576792 >50 0.32274838892307267 7.46087152781713 >100 0.05913491452674399 3.9459429397340116 >500 0.004987281948038651 1.3467560896568938 >1k 0.007124688497198071 5.955596482081944 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC 3556 0.9243902819442351 RNA PCR Primer, Index 27 (95% over 23bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3171 0.8243086569305875 No Hit GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC 3107 0.8076717114737734 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC 2807 0.7296860296449572 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG 2749 0.7146087978247194 No Hit CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT 2351 0.6111477932651566 RNA PCR Primer, Index 38 (95% over 24bp) CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC 1486 0.3862890773254031 RNA PCR Primer, Index 38 (95% over 23bp) TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC 1368 0.35561470913940196 RNA PCR Primer, Index 27 (95% over 23bp) ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC 1102 0.2864674045845183 RNA PCR Primer, Index 27 (95% over 23bp) GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT 1033 0.26853069776389055 RNA PCR Primer, Index 27 (95% over 21bp) GAATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT 955 0.24825442048839833 No Hit GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC 837 0.2175800523023973 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 824 0.2142006727564819 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGACTATGT 704 0.1830064000249554 No Hit GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT 692 0.17988697275180277 No Hit AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG 566 0.14713298638369995 RNA PCR Primer, Index 38 (95% over 22bp) AAAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC 562 0.1460931772926491 No Hit ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG 498 0.12945623183583493 RNA PCR Primer, Index 27 (95% over 22bp) AGATTTGGGAAAAGATTTTTTTTACCAACCCTACATCTGATAGAGGGCTAA 469 0.12191761592571604 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA 459 0.11931809319808884 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.6917329978215999 0.0 2 0.0 0.0 0.0 2.8563555731167756 0.0 3 0.0 0.0 0.0 3.786984709607316 0.0 4 0.0 0.0 0.0 5.385951139370811 0.0 5 0.0 0.0 0.0 9.326567642180896 0.0 6 0.0 0.0 0.0 11.796894090245031 0.0 7 0.0 0.0 0.0 13.491263004112446 0.0 8 0.0 0.0 0.0 17.222617927348537 0.0 9 0.0 0.0 0.0 18.457911127516986 0.0 10 0.0 0.0 0.0 21.711213821142437 0.0 11 0.0 0.0 0.0 24.90836682385114 0.0 12 0.0 0.0 0.0 28.45879496524438 0.0 13 0.0 0.0 0.0 29.709425349505832 0.0 14 0.0 0.0 0.0 30.17006077684137 0.0 15 0.0 0.0 0.0 30.941339170128366 0.0 16 0.0 0.0 0.0 32.3183063589525 0.0 17 0.0 0.0 0.0 33.67421741368285 0.0 18 2.5995227276272076E-4 0.0 0.0 36.34392725495599 0.0 19 2.5995227276272076E-4 0.0 0.0 37.360080689185466 0.0 20 2.5995227276272076E-4 0.0 0.0 38.380653312051905 0.0 21 2.5995227276272076E-4 0.0 0.0 39.231737053077055 0.0 22 2.5995227276272076E-4 0.0 0.0 39.94010699635547 0.0 23 2.5995227276272076E-4 0.0 0.0 40.669013169182136 0.0 24 2.5995227276272076E-4 0.0 0.0 41.17488029197839 0.0 25 2.5995227276272076E-4 0.0 0.0 41.60276173294583 0.0 26 2.5995227276272076E-4 0.0 0.0 42.01062684891054 0.0 27 2.5995227276272076E-4 0.0 0.0 42.4041945898733 0.0 28 2.5995227276272076E-4 0.0 0.0 42.770727294468735 0.0 29 2.5995227276272076E-4 0.0 0.0 43.16351517861321 0.0 30 2.5995227276272076E-4 0.0 0.0 43.616352037765864 0.0 31 2.5995227276272076E-4 0.0 0.0 44.05073228555237 0.0 32 2.5995227276272076E-4 0.0 0.0 44.44092064696922 0.0 33 2.5995227276272076E-4 0.0 0.0 44.855284569752996 0.0 34 2.5995227276272076E-4 0.0 0.0 45.2673089220819 0.0 35 2.5995227276272076E-4 0.0 0.0 45.65359799940731 0.0 36 2.5995227276272076E-4 0.0 0.0 46.01883094263893 0.0 37 2.5995227276272076E-4 0.0 0.0 46.38406388587055 0.0 38 2.5995227276272076E-4 0.0 0.0 46.74201816546482 0.0 39 2.5995227276272076E-4 0.0 0.0 47.13506600188205 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGAT 20 7.027427E-4 45.000004 36 CTTGTAG 40 6.7939254E-9 45.000004 1 TATCACG 20 7.027427E-4 45.000004 1 GCCCACA 20 7.027427E-4 45.000004 11 CAAACCG 20 7.027427E-4 45.000004 26 AATCAAT 20 7.027427E-4 45.000004 25 GAGTACT 20 7.027427E-4 45.000004 9 GTCTCAC 20 7.027427E-4 45.000004 22 CGTCTGG 20 7.027427E-4 45.000004 2 GTTAGCG 40 6.7939254E-9 45.000004 1 GTCTAAG 20 7.027427E-4 45.000004 1 AGTATCA 20 7.027427E-4 45.000004 37 ATCGTAG 20 7.027427E-4 45.000004 1 ATCAACT 20 7.027427E-4 45.000004 21 TATTGCG 20 7.027427E-4 45.000004 1 CCTCAAA 30 2.1617216E-6 45.000004 23 TACCTTA 20 7.027427E-4 45.000004 43 ATCCACT 20 7.027427E-4 45.000004 19 GTATACG 20 7.027427E-4 45.000004 1 TGTAGAC 20 7.027427E-4 45.000004 18 >>END_MODULE