FastQCFastQC Report
Sat 18 Jun 2016
SRR3548200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341319
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC121883.570853072931773No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG103803.0411433292608967No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC100792.952956032333389No Hit
GAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT34511.0110776136107278No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC33500.9814865272662817No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC26580.7787436386488886No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT20300.5947515374180752No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.5399640805229127No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTA15400.4511908214895743No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATACGTT13280.38907883827152895No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT11430.334877343482197Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT8000.23438484233224638No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGATACGTTCGT6000.1757886317491848No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC5690.16670621910881023No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTAT5080.14883437488097645No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC3930.11514155379571604No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3720.10898895168449457No Hit
ACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC3600.10547317904951088No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATG3590.10518019799659556No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG253.8850223E-545.0000044
TCACGAC253.8850223E-545.00000425
AGCGTGC253.8850223E-545.00000413
CAGAAAG351.208573E-745.0000041
CTCACGT253.8850223E-545.00000445
TCGTTAG351.208573E-745.0000041
CTGTAGG650.045.0000042
CACGGGC253.8850223E-545.0000044
CGACGGT253.8850223E-545.00000428
GCGAACT253.8850223E-545.00000433
GGCACCG1300.045.0000048
GGGTATT502.1827873E-1145.0000047
TATGGGT253.8850223E-545.0000044
GGACTAA453.8380676E-1045.0000048
GCAAAAT253.8850223E-545.0000049
TAACGGG453.8380676E-1045.0000043
TATGCGT253.8850223E-545.00000442
GTTACCA253.8850223E-545.00000431
CCACTGC253.8850223E-545.00000424
TCTACGG351.208573E-745.0000041
Produced by FastQC (version 0.11.5)