##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341319
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.71277309496395	28.0	25.0	31.0	16.0	33.0
2	27.44740257647538	30.0	25.0	31.0	16.0	33.0
3	27.429026804836532	30.0	25.0	31.0	16.0	33.0
4	30.728383125463278	33.0	28.0	35.0	19.0	37.0
5	31.94440977501985	33.0	32.0	35.0	26.0	37.0
6	31.128287613640026	35.0	30.0	35.0	17.0	36.0
7	32.07410076790334	35.0	32.0	35.0	26.0	35.0
8	32.84334010119566	35.0	32.0	35.0	28.0	37.0
9	34.05582168001195	35.0	32.0	37.0	27.0	39.0
10	33.659347999964844	35.0	32.0	37.0	27.0	39.0
11	33.85463452078554	35.0	32.0	39.0	27.0	39.0
12	33.33594965413587	35.0	31.0	37.0	25.0	39.0
13	33.299772353721885	35.0	32.0	38.0	24.0	39.0
14	34.066257665116794	36.0	32.0	39.0	24.0	40.0
15	34.83744825222153	37.0	33.0	39.0	27.0	40.0
16	35.01869512098653	36.0	33.0	39.0	27.0	41.0
17	34.55003383931161	36.0	32.0	39.0	27.0	40.0
18	34.37642498659611	36.0	32.0	39.0	27.0	40.0
19	33.73657780551332	36.0	32.0	38.0	25.0	40.0
20	33.503291642129504	35.0	32.0	38.0	25.0	40.0
21	34.18164825280749	35.0	32.0	38.0	27.0	40.0
22	34.63864009914479	36.0	33.0	39.0	27.0	40.0
23	34.92433764308462	36.0	33.0	39.0	29.0	40.0
24	34.69018425578418	36.0	33.0	39.0	27.0	40.0
25	33.93400015820977	35.0	33.0	39.0	24.0	40.0
26	33.88424611580369	35.0	32.0	39.0	24.0	40.0
27	34.27500080569789	35.0	33.0	39.0	25.0	40.0
28	33.987387165672	35.0	33.0	39.0	24.0	40.0
29	33.9669663862838	35.0	33.0	39.0	24.0	40.0
30	33.506048593837434	35.0	32.0	39.0	23.0	40.0
31	33.3191999273407	35.0	32.0	39.0	22.0	40.0
32	32.831556403247404	35.0	31.0	39.0	18.0	40.0
33	32.55998640567915	35.0	31.0	39.0	16.0	40.0
34	32.45571151913606	35.0	31.0	39.0	15.0	40.0
35	32.391299634652626	35.0	31.0	39.0	13.0	40.0
36	31.844198535680697	35.0	30.0	39.0	10.0	40.0
37	31.95506256610385	35.0	31.0	39.0	10.0	40.0
38	31.613235712046503	35.0	30.0	39.0	10.0	40.0
39	31.54177470342993	35.0	30.0	39.0	10.0	40.0
40	31.378197521966253	35.0	29.0	39.0	10.0	40.0
41	30.9855384552281	35.0	28.0	38.0	10.0	40.0
42	31.244709494636982	35.0	29.0	39.0	10.0	40.0
43	31.02947682373381	35.0	28.0	38.0	10.0	40.0
44	31.150820786419743	35.0	29.0	38.0	10.0	40.0
45	31.167339058183106	35.0	29.0	39.0	10.0	40.0
46	30.906588850899013	35.0	29.0	38.0	10.0	40.0
47	30.917065853351264	35.0	29.0	38.0	10.0	40.0
48	30.785467553813294	35.0	29.0	38.0	10.0	40.0
49	30.893612720065395	35.0	29.0	38.0	10.0	40.0
50	30.631986499433083	35.0	28.0	38.0	8.0	40.0
51	28.28754918419426	32.0	23.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	19.0
10	19.0
11	24.0
12	13.0
13	18.0
14	49.0
15	115.0
16	332.0
17	682.0
18	1333.0
19	2170.0
20	3209.0
21	4185.0
22	5568.0
23	6981.0
24	8519.0
25	10061.0
26	10845.0
27	11050.0
28	11660.0
29	13515.0
30	16335.0
31	20728.0
32	26047.0
33	31299.0
34	34788.0
35	33640.0
36	36353.0
37	33110.0
38	17038.0
39	1608.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.26526504530952	19.735789686480977	20.661316832640434	19.33762843556907
2	30.44629803790589	35.851798464193315	22.51471497338267	11.18718852451812
3	22.270075794198387	34.79355090106323	32.46288662512195	10.47348667961643
4	20.773235594854082	21.176963485771374	43.99081211418058	14.058988805193968
5	28.134677530404105	24.35112021305582	32.23318947963635	15.281012776903719
6	16.656851801394005	42.07061429337365	31.139784190156423	10.132749715075926
7	62.14919181176553	5.657757112847512	29.10005009976005	3.0930009756269063
8	58.58126854936291	15.397326254911095	19.083906843744415	6.937498351981578
9	55.275856310372404	5.8291510288029675	20.596275038893236	18.29871762193139
10	39.66787667841521	20.661316832640434	25.975700151471205	13.695106337473156
11	34.7929649389574	20.191668204817194	30.405573671550663	14.609793184674746
12	28.25948745894603	15.990319906011678	35.764197129371645	19.985995505670648
13	20.056603939423237	27.08551237991439	40.28899651059566	12.568887170066711
14	15.202200873669499	35.851798464193315	30.705293288683023	18.24070737345416
15	9.90715430433114	20.852633460194127	54.79741825096171	14.442793984513022
16	10.841470882078056	22.099560821401678	41.754487737278026	25.304480559242233
17	11.64365300496017	22.01283842973875	37.596207653251064	28.747300912050015
18	14.591628359393999	22.872737820045177	39.413569124484724	23.122064696076105
19	17.818814657256116	25.56494071528394	34.31745669007585	22.29878793738409
20	21.147372399426928	23.231346628813515	38.83229471550075	16.78898625625881
21	16.94104342272185	31.73922342442114	34.76483875787753	16.554894394979478
22	14.566724969896198	22.30230371001907	35.42521805114863	27.705753268936096
23	14.782359024841863	31.70758147070629	33.60228993990958	19.90776956454226
24	17.844596989912663	22.631028451390048	35.3748253100472	24.14954924865009
25	13.040000703154528	31.78697933604634	33.67670712735008	21.49631283344906
26	15.203079816828247	25.628517603766564	35.55237182811388	23.616030751291316
27	19.675435589580424	30.172360753430077	30.061906896481005	20.090296760508497
28	12.883548820897753	27.016075870373463	41.43191559801828	18.668459710710508
29	21.26778761217512	22.01723314553248	34.056703552981226	22.65827568931117
30	20.99765908138721	27.931348679680884	33.38196818811727	17.689024050814634
31	23.918973160005745	26.81948558386729	26.267802261227768	22.9937389948992
32	27.386696902311325	27.863377075404532	29.526044550698906	15.223881471585232
33	20.89863148550183	27.34274974437403	26.39437007608718	25.364248694036956
34	25.64287367535941	24.886396596732087	27.677334106803315	21.793395621105184
35	25.403215174074695	21.572780888259956	28.63538214983637	24.38862178782898
36	28.618682229820198	25.50136382680132	29.267049299921773	16.61290464345671
37	23.494443614331463	24.636190777542417	34.45603672810479	17.41332888002133
38	22.05326981504106	32.52001793044044	24.432275964713362	20.994436289805137
39	21.71751352840012	23.087199950779183	33.660007207333905	21.535279313486797
40	25.93526876616889	23.85598223362895	30.73224754555123	19.476501454650926
41	17.194472033493593	23.46778233851617	29.72204887509925	29.61569675289099
42	27.084633436755645	20.227997855378693	32.18953530275197	20.497833405113692
43	25.09558506851362	20.79022849592317	31.643125639064923	22.47106079649829
44	20.850875573876635	24.302778339324796	31.108435217494485	23.737910869304084
45	17.917256291035656	24.168007054983754	29.28374921993795	28.630987434042645
46	26.800441815427796	25.403215174074695	28.92015973327005	18.87618327722746
47	17.72359581505864	21.86224616854028	38.55777146891911	21.856386547481975
48	21.85726549064072	22.34420000058596	29.651147460293743	26.147387048479576
49	19.686275888538287	17.895575693119923	38.960327435624734	23.45782098271705
50	20.41491976713866	21.526196900846422	33.11887120259932	24.94001212941559
51	18.783015302400393	21.020804584567514	27.583580169870416	32.612599943161676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	102.0
2	155.0
3	2559.0
4	4963.0
5	4281.5
6	3600.0
7	3849.5
8	4099.0
9	4354.5
10	4610.0
11	4611.0
12	4612.0
13	4398.5
14	4185.0
15	4060.5
16	3936.0
17	3927.5
18	3919.0
19	3812.5
20	3706.0
21	3486.0
22	3266.0
23	3009.0
24	2752.0
25	2739.0
26	2643.5
27	2561.0
28	2933.5
29	3306.0
30	3426.0
31	3546.0
32	3813.5
33	4081.0
34	4274.0
35	4467.0
36	4984.0
37	5501.0
38	6612.0
39	7723.0
40	9757.0
41	11791.0
42	15825.0
43	19859.0
44	23378.5
45	26898.0
46	50068.5
47	73239.0
48	54414.5
49	35590.0
50	31961.5
51	28333.0
52	23554.0
53	18775.0
54	15569.5
55	12364.0
56	11026.5
57	9689.0
58	8879.0
59	8069.0
60	7316.5
61	6564.0
62	5827.0
63	5090.0
64	4108.5
65	3127.0
66	2441.0
67	1755.0
68	1416.5
69	1078.0
70	815.0
71	552.0
72	469.0
73	386.0
74	253.5
75	146.0
76	171.0
77	108.5
78	46.0
79	39.5
80	33.0
81	20.0
82	7.0
83	10.5
84	14.0
85	7.5
86	1.0
87	2.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	341319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.51144056162203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0142311533161	31.51494838237991
2	7.4018645375255305	5.553092032891337
3	2.4720988688809635	2.7819596934744393
4	1.1828692973925776	1.7748452536523716
5	0.6646868663334756	1.246668093927949
6	0.4483346957507094	1.0090608174819378
7	0.31433238197651997	0.8253742324173712
8	0.24420789496447434	0.7328471949310972
9	0.20087860820471168	0.6781721374595161
>10	2.5019150657971343	23.5299823178572
>50	0.49549888662009883	12.089153041808915
>100	0.04726539459015251	2.7599591652732824
>500	0.003151026306010167	0.7319506265933534
>1k	0.006302052612020334	5.124836381513334
>5k	0.0	0.0
>10k+	0.0023632697295076256	9.647150628337993
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC	12188	3.570853072931773	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG	10380	3.0411433292608967	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	10079	2.952956032333389	No Hit
GAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT	3451	1.0110776136107278	No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3350	0.9814865272662817	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC	2658	0.7787436386488886	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT	2030	0.5947515374180752	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1843	0.5399640805229127	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTA	1540	0.4511908214895743	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATACGTT	1328	0.38907883827152895	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	1143	0.334877343482197	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT	800	0.23438484233224638	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGATACGTTCGT	600	0.1757886317491848	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	569	0.16670621910881023	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTAT	508	0.14883437488097645	No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	393	0.11514155379571604	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	372	0.10898895168449457	No Hit
ACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	360	0.10547317904951088	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATG	359	0.10518019799659556	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.92981052915308E-4	0.0	0.0	0.39933317512356475	0.0
2	2.92981052915308E-4	0.0	0.0	2.052039294618817	0.0
3	2.92981052915308E-4	0.0	0.0	2.377248263354809	0.0
4	2.92981052915308E-4	0.0	0.0	3.979561641748628	0.0
5	2.92981052915308E-4	0.0	0.0	8.519010075618409	0.0
6	2.92981052915308E-4	0.0	0.0	9.576085714536841	0.0
7	2.92981052915308E-4	0.0	0.0	10.830044621014359	0.0
8	2.92981052915308E-4	0.0	0.0	13.255927739153108	0.0
9	2.92981052915308E-4	0.0	0.0	13.9045877903076	0.0
10	2.92981052915308E-4	0.0	0.0	18.39159261570554	0.0
11	2.92981052915308E-4	0.0	0.0	19.797022726540273	0.0
12	2.92981052915308E-4	0.0	0.0	25.148321658038373	0.0
13	2.92981052915308E-4	0.0	0.0	26.394370076087178	0.0
14	2.92981052915308E-4	0.0	0.0	26.864897647069164	0.0
15	2.92981052915308E-4	0.0	0.0	27.74120397633885	0.0
16	2.92981052915308E-4	0.0	0.0	28.604326158227348	0.0
17	2.92981052915308E-4	0.0	0.0	29.316270116811545	0.0
18	0.002050867370407156	0.0	0.0	30.758029878207775	0.0
19	0.002050867370407156	0.0	0.0	31.64576246854116	0.0
20	0.002050867370407156	0.0	0.0	32.173714325894544	0.0
21	0.002050867370407156	0.0	0.0	32.54550728204407	0.0
22	0.002050867370407156	0.0	0.0	32.93692996873892	0.0
23	0.002050867370407156	0.0	0.0	33.255986335363694	0.0
24	0.002050867370407156	0.0	0.0	33.55248316091399	0.0
25	0.002050867370407156	0.0	0.0	33.76958212112423	0.0
26	0.002050867370407156	0.0	0.0	34.00953360346186	0.0
27	0.002050867370407156	0.0	0.0	34.255344706857805	0.0
28	0.002050867370407156	0.0	0.0	34.44109469440611	0.0
29	0.002050867370407156	0.0	0.0	34.66463923778049	0.0
30	0.0026368294762377717	0.0	0.0	34.90839947380603	0.0
31	0.0026368294762377717	0.0	0.0	35.14747201298492	0.0
32	0.0026368294762377717	0.0	0.0	35.35109384476106	0.0
33	0.0026368294762377717	0.0	0.0	35.569364729182965	0.0
34	0.0029298105291530796	0.0	0.0	35.79935485572148	0.0
35	0.0029298105291530796	0.0	0.0	36.02436430436044	0.0
36	0.0029298105291530796	0.0	0.0	36.22857209824241	0.0
37	0.0029298105291530796	0.0	0.0	36.43951845634143	0.0
38	0.0029298105291530796	0.0	0.0	36.65046481444045	0.0
39	0.0029298105291530796	0.0	0.0	36.8696146420211	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	25	3.8850223E-5	45.000004	4
TCACGAC	25	3.8850223E-5	45.000004	25
AGCGTGC	25	3.8850223E-5	45.000004	13
CAGAAAG	35	1.208573E-7	45.000004	1
CTCACGT	25	3.8850223E-5	45.000004	45
TCGTTAG	35	1.208573E-7	45.000004	1
CTGTAGG	65	0.0	45.000004	2
CACGGGC	25	3.8850223E-5	45.000004	4
CGACGGT	25	3.8850223E-5	45.000004	28
GCGAACT	25	3.8850223E-5	45.000004	33
GGCACCG	130	0.0	45.000004	8
GGGTATT	50	2.1827873E-11	45.000004	7
TATGGGT	25	3.8850223E-5	45.000004	4
GGACTAA	45	3.8380676E-10	45.000004	8
GCAAAAT	25	3.8850223E-5	45.000004	9
TAACGGG	45	3.8380676E-10	45.000004	3
TATGCGT	25	3.8850223E-5	45.000004	42
GTTACCA	25	3.8850223E-5	45.000004	31
CCACTGC	25	3.8850223E-5	45.000004	24
TCTACGG	35	1.208573E-7	45.000004	1
>>END_MODULE