SRR3548199_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306716
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC	7442	2.4263488047574953	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	7072	2.305716036985354	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG	6491	2.116289988132344	No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3655	1.1916561248842577	No Hit
GAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT	2344	0.7642248855618878	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2145	0.6993440185709255	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	2124	0.6924972939135878	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC	1852	0.6038159078756895	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATACGTT	1845	0.6015336663232437	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT	1621	0.5285019366449745	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTA	1272	0.41471589353017124	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	988	0.3221220934023657	No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	977	0.3185357138199507	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT	893	0.29114881519059976	No Hit
ACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	853	0.27810743489090883	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC	626	0.2040976016901629	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	560	0.18257932419567288	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGATACGTTCGT	533	0.17377639249338148	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG	466	0.1519320804913992	No Hit
AAAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	376	0.12258897481709465	No Hit
AGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG	357	0.11639431917474145	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATG	339	0.11052569803988053	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTAT	327	0.10661328394997326	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGTT	30	2.160279E-6	45.000004	42
AAGTATG	30	2.160279E-6	45.000004	1
ATAATGG	30	2.160279E-6	45.000004	2
CATCTTG	30	2.160279E-6	45.000004	41
AATGGGC	30	2.160279E-6	45.000004	4
ATACTAT	30	2.160279E-6	45.000004	45
GGGACGA	30	2.160279E-6	45.000004	7
CAAGGGT	30	2.160279E-6	45.000004	4
GCCCTAG	30	2.160279E-6	45.000004	20
GCTAGAG	30	2.160279E-6	45.000004	1
CTGGGTC	30	2.160279E-6	45.000004	5
TAGTGGT	30	2.160279E-6	45.000004	15
TATACTA	30	2.160279E-6	45.000004	44
CGCCGGT	30	2.160279E-6	45.000004	28
AATCGAG	30	2.160279E-6	45.000004	1
TCACGAC	25	3.884096E-5	45.0	25
TATTTGA	20	7.02519E-4	45.0	32
ACAACAT	20	7.02519E-4	45.0	39
TTCTAGG	35	1.2080272E-7	45.0	2
GAGTGTG	20	7.02519E-4	45.0	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content