FastQCFastQC Report
Sat 18 Jun 2016
SRR3548195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688009
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.7383624342123432No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC26130.37979154342457727No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT22900.3328444831390287TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG17520.2546478316417372No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC17060.24796187259178296No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC16510.23996779111901154No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC14400.20929958765074294No Hit
TCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC13800.20057877149862866No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11600.16860244560754292No Hit
ACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC10750.15624795605871433TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG253.8891667E-545.0000041
TCGCAAG302.1642263E-645.0000041
CGAAATC302.1642263E-645.00000413
CGAGTAG253.8891667E-545.0000042
CGAAAGG1150.045.0000042
CGACGAT253.8891667E-545.00000434
CCCTATC253.8891667E-545.00000428
CGTGCCG302.1642263E-645.00000441
TGACGCA253.8891667E-545.00000426
ATGTACG302.1642263E-645.0000041
TGCATCG302.1642263E-645.00000424
TAACCGC253.8891667E-545.00000425
AGTGATC253.8891667E-545.00000412
CGCTAGG253.8891667E-545.0000042
GCTTACG253.8891667E-545.00000420
AAACCGT253.8891667E-545.00000438
TTACGAA302.1642263E-645.00000412
ACGTAAG302.1642263E-645.0000041
ATCATAC253.8891667E-545.00000415
ATGCACG302.1642263E-645.0000041
Produced by FastQC (version 0.11.5)