SRR3548193_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556625
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	13719	2.4646754996631484	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	12798	2.299214013024927	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	10649	1.913137210869077	No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	5031	0.9038401077924995	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3982	0.7153828879407141	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC	3927	0.7055019088255109	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	3816	0.6855602964293734	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT	3245	0.5829777677969908	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	2991	0.5373456097013249	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	2795	0.5021333932180553	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	2508	0.45057264765326743	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA	2355	0.42308556029642935	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT	1501	0.2696609027621828	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1470	0.26409162362452276	No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1298	0.2331911071187963	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	1190	0.2137884572198518	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	964	0.17318661576465305	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGT	935	0.167976644958455	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	784	0.14084886593307883	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATG	707	0.12701549517179428	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	691	0.12414102852009881	No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	678	0.12180552436559622	No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	654	0.117493824388053	No Hit
AAAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	645	0.1158769368964743	No Hit
AGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	615	0.11048731192454524	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	578	0.10384010779249944	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	20	7.030146E-4	45.000004	21
TCGCCAA	20	7.030146E-4	45.000004	40
CAGGTCT	40	6.8048394E-9	45.000004	5
ACTATTA	30	2.163477E-6	45.000004	17
TCGTTTG	20	7.030146E-4	45.000004	12
CGAACTC	20	7.030146E-4	45.000004	13
GTACAAT	20	7.030146E-4	45.000004	24
CATGCTT	20	7.030146E-4	45.000004	10
CGACGGT	20	7.030146E-4	45.000004	28
TGGACAG	20	7.030146E-4	45.000004	1
ACCGCAA	20	7.030146E-4	45.000004	29
CGCATTG	20	7.030146E-4	45.000004	28
CGTTGGT	20	7.030146E-4	45.000004	14
CGACATG	20	7.030146E-4	45.000004	18
CACGACG	20	7.030146E-4	45.000004	26
TAACGAT	20	7.030146E-4	45.000004	13
GCGTTGC	20	7.030146E-4	45.000004	20
GCCGATT	20	7.030146E-4	45.000004	9
ACGATAT	20	7.030146E-4	45.000004	15
AAGCCGT	20	7.030146E-4	45.000004	12

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content